Restriction map for
pFN21AB3963
(Circular) (Six-base) MAPSORT of: pFN21AB3963.seq Check: 7880 from: 1 to: 6844
pFN21AB3963 6844 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2953 2953
Size: 6844
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3366 4654 278
Size: 53 83 186 936 1830 1288 2468
Fragments arranged by size:
2468 1830 1288 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3642 4187 1937
Size: 1705 545 4594
Fragments arranged by size:
4594 1705 545
Acc65I G'GTAC_C
Cuts at: 4165 4165
Size: 6844
AclI AA'CG_TT
Cuts at: 1563 4971 5344 1563
Size: 3408 373 3063
Fragments arranged by size:
3408 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2152 3795 4078 4111 4907 6291 6559 1632
Size: 520 1643 283 33 796 1384 268 1917
Fragments arranged by size:
1917 1643 1384 796 520 283 268 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6647
AflIII A'CryG_T
Cuts at: 1203 2895 5743 6720 1203
Size: 1692 2848 977 1327
Fragments arranged by size:
2848 1692 1327 977
AgeI A'CCGG_T
Cuts at: 5675 5675
Size: 6844
AhdI GACnn_n'nnGTC
Cuts at: 3628 5573 3628
Size: 1945 4899
AleI CACnn'nnGTG
Cuts at: 1166 2792 2936 3614 1166
Size: 1626 144 678 4396
Fragments arranged by size:
4396 1626 678 144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3506 1184
Size: 2322 4522
AlwNI CAG_nnn'CTG
Cuts at: 2053 2175 3773 4587 6159 2053
Size: 122 1598 814 1572 2738
Fragments arranged by size:
2738 1598 1572 814 122
ApaI G_GGCC'C
Cuts at: 3428 4087 3428
Size: 659 6185
ApaLI G'TGCA_C
Cuts at: 4901 6057 4901
Size: 1156 5688
ApoI r'AATT_y
Cuts at: 1493 2132 2381 2562 3089 3303 3860 4054 4153
Size: 639 249 181 527 214 557 194 99
Cuts at: 4153 4379 4531 6640 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 639 557 527 249 226 214
194 181 152 99
AseI AT'TA_AT
Cuts at: 160 1032 5398 160
Size: 872 4366 1606
Fragments arranged by size:
4366 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6844
AvaI C'yCGr_G
Cuts at: 1870 1943 2209 2678 3224 4169 1870
Size: 73 266 469 546 945 4545
Fragments arranged by size:
4545 945 546 469 266 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4025 4058 4025
Size: 33 6811
BamHI G'GATC_C
Cuts at: 4174 4541 4174
Size: 367 6477
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2689 2786 4165 5620 618
Size: 325 217 627 902 97 1379 1455 1842
Fragments arranged by size:
1842 1455 1379 902 627 325 217 97
BanII G_rGCy'C
Cuts at: 1391 2216 2363 3428 4087 4163 1391
Size: 825 147 1065 659 76 4072
Fragments arranged by size:
4072 1065 825 659 147 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3351 3424 928
Size: 971 1452 73 4348
Fragments arranged by size:
4348 1452 971 73
BbvCI CC'TCA_GC
Cuts at: 4118 4118
Size: 6844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2473 2507 2659 2693 5035
Size: 34 50 34 452 34 152 34 2342
Cuts at: 5035 5069 1903
Size: 34 3678
Fragments arranged by size:
3678 2342 452 152 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4736 5946 4736
Size: 1210 5634
BclI T'GATC_A
Cuts at: 1548 2713 2991 3076 3327 1548
Size: 1165 278 85 251 5065
Fragments arranged by size:
5065 1165 278 251 85
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5455 136
Size: 107 122 71 631 605 3783 1525
Fragments arranged by size:
3783 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3903 4024 6839 3903
Size: 121 2815 3908
Fragments arranged by size:
3908 2815 121
BlpI GC'TnA_GC
Cuts at: 4132 4261 4132
Size: 129 6715
Bme1580I G_kGCm'C
Cuts at: 1790 3428 3707 4087 4905 6061 1790
Size: 1638 279 380 818 1156 2573
Fragments arranged by size:
2573 1638 1156 818 380 279
BmgBI CAC'GTC
Cuts at: 1318 2764 3736 1318
Size: 1446 972 4426
Fragments arranged by size:
4426 1446 972
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3422 3717 4575
Size: 416 53 83 399 24 2005 295 858
Cuts at: 4575 5533 442
Size: 958 1753
Fragments arranged by size:
2005 1753 958 858 416 399 295 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6844
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3490 3490
Size: 6844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3749 5504 1239
Size: 2510 1755 2579
Fragments arranged by size:
2579 2510 1755
Bpu10I CC'TnA_GC
Cuts at: 4118 4118
Size: 6844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2988 4969 5834 6132 2988
Size: 1981 865 298 3700
Fragments arranged by size:
3700 1981 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2212 2645 5507 882
Size: 617 713 433 2862 2219
Fragments arranged by size:
2862 2219 713 617 433
BsaAI yAC'GTr
Cuts at: 493 1204 4635 493
Size: 711 3431 2702
Fragments arranged by size:
3431 2702 711
BsaBI GATnn'nnATC
Cuts at: 3059 3831 6838 3059
Size: 772 3007 3065
Fragments arranged by size:
3065 3007 772
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2873 3363 4651 5033
Size: 53 83 186 936 1340 490 1288 382
Cuts at: 5033 5668 275
Size: 635 1451
Fragments arranged by size:
1451 1340 1288 936 635 490 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5277 5675 5949 6096 6507 5277
Size: 398 274 147 411 5614
Fragments arranged by size:
5614 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2913 2943 3129 3159 3503
Size: 30 165 30 1510 30 186 30 344
Cuts at: 3503 3533 1178
Size: 30 4489
Fragments arranged by size:
4489 1510 344 186 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4106 1186
Size: 2920 3924
BseYI C'CCAG_C
Cuts at: 2827 3377 3562 6047 6543 2827
Size: 550 185 2485 496 3128
Fragments arranged by size:
3128 2485 550 496 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2068 1163
Size: 573 332 5939
Fragments arranged by size:
5939 573 332
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2732 4294 5055 6083 1540
Size: 275 186 731 1562 761 1028 2301
Fragments arranged by size:
2301 1562 1028 761 731 275 186
BsiHKAI G_wGCw'C
Cuts at: 4046 4905 4990 6061 4046
Size: 859 85 1071 4829
Fragments arranged by size:
4829 1071 859 85
BsmI GAATG_Cn'
Cuts at: 3508 4355 4448 3508
Size: 847 93 5904
Fragments arranged by size:
5904 847 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2599 3370 2599
Size: 771 6073
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2363 3428 3707 4046 4087 4163
Size: 399 426 147 1065 279 339 41 76
Cuts at: 4163 4905 4990 6061 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1065 742 426 399 339 279
147 85 76 41
BspEI T'CCGG_A
Cuts at: 6507 6507
Size: 6844
BspHI T'CATG_A
Cuts at: 3057 4733 6463 3057
Size: 1676 1730 3438
Fragments arranged by size:
3438 1730 1676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2953 3557 3981 4199 844
Size: 2109 604 424 218 3489
Fragments arranged by size:
3489 2109 604 424 218
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 4731 1394
Size: 429 200 2708 3507
Fragments arranged by size:
3507 2708 429 200
BsrDI GCAATG_nn'
Cuts at: 65 1248 5339 5513 65
Size: 1183 4091 174 1396
Fragments arranged by size:
4091 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2686 5488 5675 1700
Size: 110 876 2802 187 2869
Fragments arranged by size:
2869 2802 876 187 110
BsrGI T'GTAC_A
Cuts at: 96 3654 96
Size: 3558 3286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6844
BssSI C'ACGA_G
Cuts at: 2330 4904 5916 2330
Size: 2574 1012 3258
Fragments arranged by size:
3258 2574 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6844
BstBI TT'CG_AA
Cuts at: 5648 5648
Size: 6844
BstEII G'GTnAC_C
Cuts at: 1184 2775 3454 1184
Size: 1591 679 4574
Fragments arranged by size:
4574 1591 679
BstXI CCAn_nnnn'nTGG
Cuts at: 3570 3570
Size: 6844
BstYI r'GATC_y
Cuts at: 1972 3832 3850 3903 4024 4174 4541 4926 4943
Size: 1860 18 53 121 150 367 385 17
Cuts at: 4943 6395 6481 6493 6839 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1860 1452 385 367 346 150 121
86 53 18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3507 513
Size: 552 2442 3850
Fragments arranged by size:
3850 2442 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2250 487
Size: 683 56 1024 5081
Fragments arranged by size:
5081 1024 683 56
BtsI GCAGTG_nn'
Cuts at: 2277 3657 4360 5154 5174 2277
Size: 1380 703 794 20 3947
Fragments arranged by size:
3947 1380 794 703 20
ClaI AT'CG_AT
Cuts at: 1554 2907 3063 1554
Size: 1353 156 5335
Fragments arranged by size:
5335 1353 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6809
DraI TTT'AAA
Cuts at: 2380 4148 2380
Size: 1768 5076
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5851 809
Size: 507 4535 1802
Fragments arranged by size:
4535 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2758 3042 4291
Size: 54 1425 50 275 18 928 284 1249
Cuts at: 4291 5180 6809 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1249 928 889 284 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4291 1537
Size: 275 2479 4090
Fragments arranged by size:
4090 2479 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3671 4130 4774 1353
Size: 2318 459 644 3423
Fragments arranged by size:
3423 2318 644 459
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5431 5805 5951 387
Size: 1064 138 3842 374 146 1280
Fragments arranged by size:
3842 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 3749 3795 4078 4111 4907 5504
Size: 393 520 1597 46 283 33 796 597
Cuts at: 5504 6291 6559 1239
Size: 787 268 1524
Fragments arranged by size:
1597 1524 796 787 597 520 393 283
268 46 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 2916 2045
Size: 871 5973
EcoO109I rG'GnC_Cy
Cuts at: 2747 3424 3469 3559 4083 4084 2747
Size: 677 45 90 524 1 5507
Fragments arranged by size:
5507 677 524 90 45 1
EcoRI G'AATT_C
Cuts at: 2132 4054 4153 4531 2132
Size: 1922 99 378 4445
Fragments arranged by size:
4445 1922 378 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3290 3290
Size: 6844
FspI TGC'GCA
Cuts at: 5350 5350
Size: 6844
HaeII r_GCGC'y
Cuts at: 1678 5991 1678
Size: 4313 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 2610 2642 2870
Size: 32 135 32 560 32 446 32 228
Cuts at: 2870 2902 2913 2945 3129 3161 3503 3535 3614
Size: 32 11 32 184 32 342 32 79
Cuts at: 3614 3646 5485 5517 5559 5591 1373
Size: 32 1839 32 42 32 2626
Fragments arranged by size:
2626 1839 560 446 342 228 184 135
79 42 32 32 32 32 32 32
32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3418 3604 3643 4188 4434 669
Size: 988 281 1480 186 39 545 246 3079
Fragments arranged by size:
3079 1480 988 545 281 246 186 39
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6844
HpaI GTT'AAC
Cuts at: 4434 4434
Size: 6844
KpnI G_GTAC'C
Cuts at: 4169 4169
Size: 6844
MfeI C'AATT_G
Cuts at: 3675 4443 3675
Size: 768 6076
MluI A'CGCG_T
Cuts at: 6720 6720
Size: 6844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3007 5958 6142 1305
Size: 1702 2951 184 2007
Fragments arranged by size:
2951 2007 1702 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5022
Fragments arranged by size:
5022 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2792 2795 2936 3056 3452 3614 3983
Size: 648 1626 3 141 120 396 162 369
Cuts at: 3983 4802 5161 5320 518
Size: 819 359 159 2042
Fragments arranged by size:
2042 1626 819 648 396 369 359 162
159 141 120 3
MspA1I CmG'CkG
Cuts at: 1606 2129 3614 4260 4937 6085 6330 1606
Size: 523 1485 646 677 1148 245 2120
Fragments arranged by size:
2120 1485 1148 677 646 523 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6292
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6844
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6844
NotI GC'GGCC_GC
Cuts at: 4291 4291
Size: 6844
NspI r_CATG'y
Cuts at: 1132 1229 2899 4202 5747 1132
Size: 97 1670 1303 1545 2229
Fragments arranged by size:
2229 1670 1545 1303 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6844
PciI A'CATG_T
Cuts at: 2895 5743 2895
Size: 2848 3996
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2767 1108
Size: 1659 5185
PfoI T'CCnGG_A
Cuts at: 2089 3408 2089
Size: 1319 5525
PmeI GTTT'AAAC
Cuts at: 4148 4148
Size: 6844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4913 4945 6426 6458 4913
Size: 32 1481 32 5299
Fragments arranged by size:
5299 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3469 3559 3469
Size: 90 6754
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6844
PsiI TTA'TAA
Cuts at: 4414 4414
Size: 6844
PspOMI G'GGCC_C
Cuts at: 3424 4083 3424
Size: 659 6185
PstI C_TGCA'G
Cuts at: 830 2071 2272 3528 4052 4196 830
Size: 1241 201 1256 524 144 3478
Fragments arranged by size:
3478 1256 1241 524 201 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6844
PvuII CAG'CTG
Cuts at: 2129 3614 2129
Size: 1485 5359
SalI G'TCGA_C
Cuts at: 1936 3641 4186 1936
Size: 1705 545 4594
Fragments arranged by size:
4594 1705 545
SbfI CC_TGCA'GG
Cuts at: 4052 4196 4052
Size: 144 6700
ScaI AGT'ACT
Cuts at: 5092 6804 5092
Size: 1712 5132
SfcI C'TryA_G
Cuts at: 826 1045 2067 2268 3524 4048 4192 5327 6008
Size: 219 1022 201 1256 524 144 1135 681
Cuts at: 6008 6199 826
Size: 191 1471
Fragments arranged by size:
1471 1256 1135 1022 681 524 219 201
191 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6844
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6844
SmaI CCC'GGG
Cuts at: 1872 3226 4171 1872
Size: 1354 945 4545
Fragments arranged by size:
4545 1354 945
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2209 2678 3003 4948 5849 6111
Size: 197 926 266 469 325 1945 901 262
Cuts at: 6111 820
Size: 1553
Fragments arranged by size:
1945 1553 926 901 469 325 266 262
197
SnaBI TAC'GTA
Cuts at: 493 4635 493
Size: 4142 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6844
SphI G_CATG'C
Cuts at: 1132 4202 1132
Size: 3070 3774
SspI AAT'ATT
Cuts at: 5 52 3580 4768 5
Size: 47 3528 1188 2081
Fragments arranged by size:
3528 2081 1188 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6844
StyI C'CwwG_G
Cuts at: 513 1065 2610 4283 513
Size: 552 1545 1673 3074
Fragments arranged by size:
3074 1673 1545 552
SwaI ATTT'AAAT
Cuts at: 2380 2380
Size: 6844
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2746 2794 2973 4021 4871 4888 5041
Size: 1255 1166 48 179 1048 850 17 153
Cuts at: 5041 5226 5671 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1166 1048 850 445 185 179
153 48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3654 5090 6802
Size: 274 80 33 51 1443 1677 1436 1712
Cuts at: 6802 96
Size: 138
Fragments arranged by size:
1712 1677 1443 1436 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2526 2558 6804 6836 2526
Size: 32 4246 32 2534
Fragments arranged by size:
4246 2534 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2927 1316
Size: 1611 5233
XbaI T'CTAG_A
Cuts at: 4180 4180
Size: 6844
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3616 3616
Size: 6844
XhoI C'TCGA_G
Cuts at: 1943 2209 2678 1943
Size: 266 469 6109
Fragments arranged by size:
6109 469 266
XmaI C'CCGG_G
Cuts at: 1870 3224 4169 1870
Size: 1354 945 4545
Fragments arranged by size:
4545 1354 945
XmnI GAAnn'nnTTC
Cuts at: 2108 4973 2108
Size: 2865 3979
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3364 4652 276
Size: 53 83 186 936 1830 1288 2468
Fragments arranged by size:
2468 1830 1288 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI