Restriction map for
pFN21AB3955
(Circular) (Six-base) MAPSORT of: pFN21AB3955.seq Check: 1590 from: 1 to: 6067
pFN21AB3955 6067 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2629 3877 278
Size: 53 83 186 936 1093 1248 2468
Fragments arranged by size:
2468 1248 1093 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3168 3410 1937
Size: 1231 242 4594
Fragments arranged by size:
4594 1231 242
Acc65I G'GTAC_C
Cuts at: 2191 3388 2191
Size: 1197 4870
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2924 3060 3257 4130 5514 5782 1632
Size: 1292 136 197 873 1384 268 1917
Fragments arranged by size:
1917 1384 1292 873 268 197 136
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 3360 4966 5943 1203
Size: 2157 1606 977 1327
Fragments arranged by size:
2157 1606 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 2435 1166
Size: 1269 4798
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 3810 5382 3810
Size: 1572 4495
ApaI G_GGCC'C
Cuts at: 2828 2828
Size: 6067
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 2102 2333 2867 2983 3020 3376 3602 3754
Size: 609 231 534 116 37 356 226 152
Cuts at: 3754 5863 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 609 534 356 231 226 152
116 37
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 3392 1870
Size: 73 1449 4545
Fragments arranged by size:
4545 1449 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 3388 4843 618
Size: 325 217 627 404 1197 1455 1842
Fragments arranged by size:
1842 1455 1197 627 404 325 217
BanII G_rGCy'C
Cuts at: 1391 2828 3386 1391
Size: 1437 558 4072
Fragments arranged by size:
4072 1437 558
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3245 928
Size: 971 1346 3750
Fragments arranged by size:
3750 1346 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4258 4292 1903
Size: 34 2321 34 3678
Fragments arranged by size:
3678 2321 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 2377 1548
Size: 829 5238
BfrBI ATG'CAT
Cuts at: 2456 2456
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4678 136
Size: 107 122 71 631 605 3006 1525
Fragments arranged by size:
3006 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2116 2149 6062 2116
Size: 33 3913 2121
Fragments arranged by size:
3913 2121 33
BlpI GC'TnA_GC
Cuts at: 3484 3484
Size: 6067
Bme1580I G_kGCm'C
Cuts at: 1790 2828 4128 5284 1790
Size: 1038 1300 1156 2573
Fragments arranged by size:
2573 1300 1156 1038
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2699 3798 4756
Size: 416 53 83 399 24 1282 1099 958
Cuts at: 4756 442
Size: 1753
Fragments arranged by size:
1753 1282 1099 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3174 4727 1239
Size: 1935 1553 2579
Fragments arranged by size:
2579 1935 1553
Bpu10I CC'TnA_GC
Cuts at: 2187 2187
Size: 6067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4192 5057 5355 4192
Size: 865 298 4904
Fragments arranged by size:
4904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4730 882
Size: 617 3231 2219
Fragments arranged by size:
3231 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3361 3858 493
Size: 711 2157 497 2702
Fragments arranged by size:
2702 2157 711 497
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2626 3874 4256 4891
Size: 53 83 186 936 1093 1248 382 635
Cuts at: 4891 275
Size: 1451
Fragments arranged by size:
1451 1248 1093 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4500 4898 5172 5319 5730 4500
Size: 398 274 147 411 4837
Fragments arranged by size:
4837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2053 2083 1178
Size: 30 165 30 650 30 5162
Fragments arranged by size:
5162 650 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2751 3281 1186
Size: 1565 530 3972
Fragments arranged by size:
3972 1565 530
BseYI C'CCAG_C
Cuts at: 2500 5270 5766 2500
Size: 2770 496 2801
Fragments arranged by size:
2801 2770 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5494
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4128 4213 5284 4128
Size: 85 1071 4911
Fragments arranged by size:
4911 1071 85
BsmI GAATG_Cn'
Cuts at: 3063 3578 3671 3063
Size: 515 93 5459
Fragments arranged by size:
5459 515 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2828 3386 4128 4213 5284 1391
Size: 399 1038 558 742 85 1071 2174
Fragments arranged by size:
2174 1071 1038 742 558 399 85
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 2125 3956 5686 2125
Size: 1831 1730 2506
Fragments arranged by size:
2506 1831 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3422 844
Size: 2578 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3954 1394
Size: 429 2131 3507
Fragments arranged by size:
3507 2131 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4711 4898 1700
Size: 110 2901 187 2869
Fragments arranged by size:
2901 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2358 96
Size: 2262 3805
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 2041 1184
Size: 857 5210
BstYI r'GATC_y
Cuts at: 1972 2116 2149 3397 3764 4149 4166 5618 5704
Size: 144 33 1248 367 385 17 1452 86
Cuts at: 5704 5716 6062 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1248 385 367 346 144 86
33 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2547 2724 3204 513
Size: 552 939 543 177 480 3376
Fragments arranged by size:
3376 939 552 543 480 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2502 487
Size: 683 56 1276 4052
Fragments arranged by size:
4052 1276 683 56
BtsI GCAGTG_nn'
Cuts at: 2444 2740 3230 3583 4377 4397 2444
Size: 296 490 353 794 20 4114
Fragments arranged by size:
4114 794 490 353 296 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 2332 3025 3371 2332
Size: 693 346 5028
Fragments arranged by size:
5028 693 346
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2739 3514 4403
Size: 54 1425 50 275 18 909 775 889
Cuts at: 4403 6032 8
Size: 1629 43
Fragments arranged by size:
1629 1425 909 889 775 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2970 3036 3997 1353
Size: 1617 66 961 3423
Fragments arranged by size:
3423 1617 961 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2924 3060 3174 3257 4130 4727 5514
Size: 393 1292 136 114 83 873 597 787
Cuts at: 5514 5782 1239
Size: 268 1524
Fragments arranged by size:
1524 1292 873 787 597 393 268 136
114 83
EcoO109I rG'GnC_Cy
Cuts at: 2403 2824 2825 2912 2403
Size: 421 1 87 5558
Fragments arranged by size:
5558 421 87 1
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2259 2259
Size: 6067
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 5214 1678
Size: 3536 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4708 4740 4782 4814 1373
Size: 32 135 32 3136 32 42 32 2626
Fragments arranged by size:
3136 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2749 748
Size: 2001 4066
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KpnI G_GTAC'C
Cuts at: 2195 3392 2195
Size: 1197 4870
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2433 5181 5365 1305
Size: 1128 2748 184 2007
Fragments arranged by size:
2748 2007 1128 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4245
Fragments arranged by size:
4245 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 3294 4025 4384 4543 518
Size: 648 1269 859 731 359 159 2042
Fragments arranged by size:
2042 1269 859 731 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3483 4160 5308 5553 1606
Size: 1877 677 1148 245 2120
Fragments arranged by size:
2120 1877 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2547 2724 3204 513
Size: 552 939 543 177 480 3376
Fragments arranged by size:
3376 939 552 543 480 177
NdeI CA'TA_TG
Cuts at: 387 2797 387
Size: 2410 3657
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NsiI A_TGCA'T
Cuts at: 2458 2458
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 3316 3425 4970 1132
Size: 97 2087 109 1545 2229
Fragments arranged by size:
2229 2087 1545 109 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6067
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6067
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2912 2912
Size: 6067
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PspOMI G'GGCC_C
Cuts at: 2824 2824
Size: 6067
PstI C_TGCA'G
Cuts at: 830 2315 3419 830
Size: 1485 1104 3478
Fragments arranged by size:
3478 1485 1104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SapI GCTCTTCn'nnn_
Cuts at: 2970 2970
Size: 6067
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SfcI C'TryA_G
Cuts at: 826 1045 2311 3415 4550 5231 5422 826
Size: 219 1266 1104 1135 681 191 1471
Fragments arranged by size:
1471 1266 1135 1104 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4171 5072 5334 820
Size: 197 926 2228 901 262 1553
Fragments arranged by size:
2228 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6067
StyI C'CwwG_G
Cuts at: 513 1065 2004 2547 2615 2724 3204 3506 513
Size: 552 939 543 68 109 480 302 3074
Fragments arranged by size:
3074 939 552 543 480 302 109 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4094 4111 4264 4449 4894 325
Size: 1255 2514 17 153 185 445 1498
Fragments arranged by size:
2514 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2358 4313 6025
Size: 274 80 33 51 1443 381 1955 1712
Cuts at: 6025 96
Size: 138
Fragments arranged by size:
1955 1712 1443 381 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6027 6059 6027
Size: 32 6035
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6067
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 2142 4196 2142
Size: 2054 4013
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2627 3875 276
Size: 53 83 186 936 1093 1248 2468
Fragments arranged by size:
2468 1248 1093 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI