Restriction map for
pFN21AB3954
(Circular) (Six-base) MAPSORT of: pFN21AB3954.seq Check: 3478 from: 1 to: 9103
pFN21AB3954 9103 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2945 3287 2945
Size: 342 8761
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6913 278
Size: 53 83 186 936 5377 2468
Fragments arranged by size:
5377 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2707 3688 6446 1937
Size: 770 981 2758 4594
Fragments arranged by size:
4594 2758 981 770
Acc65I G'GTAC_C
Cuts at: 6424 6424
Size: 9103
AclI AA'CG_TT
Cuts at: 1563 7230 7603 1563
Size: 5667 373 3063
Fragments arranged by size:
5667 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2622 6063 6194 6231 7166 8550 8818 1632
Size: 990 3441 131 37 935 1384 268 1917
Fragments arranged by size:
3441 1917 1384 990 935 268 131 37
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8906
AflIII A'CryG_T
Cuts at: 1203 5977 8002 8979 1203
Size: 4774 2025 977 1327
Fragments arranged by size:
4774 2025 1327 977
AgeI A'CCGG_T
Cuts at: 7934 7934
Size: 9103
AhdI GACnn_n'nnGTC
Cuts at: 7832 7832
Size: 9103
AleI CACnn'nnGTG
Cuts at: 1166 3135 1166
Size: 1969 7134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9103
AlwNI CAG_nnn'CTG
Cuts at: 3446 3545 4256 4427 4724 5372 5408 6846 8418
Size: 99 711 171 297 648 36 1438 1572
Cuts at: 8418 3446
Size: 4131
Fragments arranged by size:
4131 1572 1438 711 648 297 171 99
36
ApaI G_GGCC'C
Cuts at: 3555 4078 4716 4823 3555
Size: 523 638 107 7835
Fragments arranged by size:
7835 638 523 107
ApaLI G'TGCA_C
Cuts at: 4002 7160 8316 4002
Size: 3158 1156 4789
Fragments arranged by size:
4789 3158 1156
ApoI r'AATT_y
Cuts at: 1493 2238 5801 6200 6224 6368 6412 6638 6790
Size: 745 3563 399 24 144 44 226 152
Cuts at: 6790 8899 1493
Size: 2109 1697
Fragments arranged by size:
3563 2109 1697 745 399 226 152 144
44 24
AseI AT'TA_AT
Cuts at: 160 1032 3168 7657 160
Size: 872 2136 4489 1606
Fragments arranged by size:
4489 2136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9103
AvaI C'yCGr_G
Cuts at: 1870 1943 2961 2979 3240 3717 5247 5499 6428
Size: 73 1018 18 261 477 1530 252 929
Cuts at: 6428 1870
Size: 4545
Fragments arranged by size:
4545 1530 1018 929 477 261 252 73
18
AvrII C'CTAG_G
Cuts at: 4904 4904
Size: 9103
BamHI G'GATC_C
Cuts at: 2177 3038 6433 6800 2177
Size: 861 3395 367 4480
Fragments arranged by size:
4480 3395 861 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3092 3317 3461 4037 4352
Size: 325 217 627 1305 225 144 576 315
Cuts at: 4352 4811 5126 5351 5576 6424 7879 618
Size: 459 315 225 225 848 1455 1842
Fragments arranged by size:
1842 1455 1305 848 627 576 459 325
315 315 225 225 225 217 144
BanII G_rGCy'C
Cuts at: 1391 2890 2917 3223 3241 3333 3555 4015 4078
Size: 1499 27 306 18 92 222 460 63
Cuts at: 4078 4492 4716 4823 4834 5534 6422 1391
Size: 414 224 107 11 700 888 4072
Fragments arranged by size:
4072 1499 888 700 460 414 306 224
222 107 92 63 27 18 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2861 4634 4994 5057 928
Size: 971 962 1773 360 63 4974
Fragments arranged by size:
4974 1773 971 962 360 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7294 7328 1903
Size: 34 5357 34 3678
Fragments arranged by size:
5357 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2591 2763 6995 8205 2591
Size: 172 4232 1210 3489
Fragments arranged by size:
4232 3489 1210 172
BclI T'GATC_A
Cuts at: 1548 6361 1548
Size: 4813 4290
BfrBI ATG'CAT
Cuts at: 5910 5910
Size: 9103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2541 3874 4323
Size: 107 122 71 631 605 869 1333 449
Cuts at: 4323 5214 7714 136
Size: 891 2500 1525
Fragments arranged by size:
2500 1525 1333 891 869 631 605 449
122 107 71
BglII A'GATC_T
Cuts at: 9098 9098
Size: 9103
BlpI GC'TnA_GC
Cuts at: 6520 6520
Size: 9103
Bme1580I G_kGCm'C
Cuts at: 1790 3097 3466 3555 4006 4042 4078 4357 4716
Size: 1307 369 89 451 36 36 279 359
Cuts at: 4716 4816 4823 5131 5356 5581 7164 8320 1790
Size: 100 7 308 225 225 1583 1156 2573
Fragments arranged by size:
2573 1583 1307 1156 451 369 359 308
279 225 225 100 89 36 36 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2458 3882 5430
Size: 416 53 83 399 24 1041 1424 1548
Cuts at: 5430 5845 5907 6276 6834 7792 442
Size: 415 62 369 558 958 1753
Fragments arranged by size:
1753 1548 1424 1041 958 558 416 415
399 369 83 62 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2511 2577 2631 2730 2742 3234 3237 3435
Size: 1272 66 54 99 12 492 3 198
Cuts at: 3435 3522 3543 3588 3615 3822 3936 3999 4029
Size: 87 21 45 27 207 114 63 30
Cuts at: 4029 4209 4353 4404 4542 4560 4683 4776 4818
Size: 180 144 51 138 18 123 93 42
Cuts at: 4818 4884 5349 6070 6263 7763 1239
Size: 66 465 721 193 1500 2579
Fragments arranged by size:
2579 1500 1272 721 492 465 207 198
193 180 144 138 123 114 99 93
87 66 66 63 54 51 45 42
30 27 21 18 12 3
Bpu10I CC'TnA_GC
Cuts at: 3051 3051
Size: 9103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2301 3126 5250 5702 5804 7228 8093 8391 2301
Size: 825 2124 452 102 1424 865 298 3013
Fragments arranged by size:
3013 2124 1424 865 825 452 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3081 4970 5787 7766 882
Size: 617 1582 1889 817 1979 2219
Fragments arranged by size:
2219 1979 1889 1582 817 617
BsaAI yAC'GTr
Cuts at: 493 1204 3341 5276 6894 493
Size: 711 2137 1935 1618 2702
Fragments arranged by size:
2702 2137 1935 1618 711
BsaBI GATnn'nnATC
Cuts at: 9097 9097
Size: 9103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3588 6910 7292 7927
Size: 53 83 186 936 2055 3322 382 635
Cuts at: 7927 275
Size: 1451
Fragments arranged by size:
3322 2055 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7536 7934 8208 8355 8766 7536
Size: 398 274 147 411 7873
Fragments arranged by size:
7873 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2281 2311 2350 2380 2484
Size: 30 165 30 878 30 39 30 104
Cuts at: 2484 2514 2827 2857 4402 4432 4726 4756 1178
Size: 30 313 30 1545 30 294 30 5525
Fragments arranged by size:
5525 1545 878 313 294 165 104 39
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3123 3573 4212 5211 1186
Size: 1937 450 639 999 5078
Fragments arranged by size:
5078 1937 999 639 450
BseYI C'CCAG_C
Cuts at: 2765 4318 4688 4853 5621 8306 8802 2765
Size: 1553 370 165 768 2685 496 3066
Fragments arranged by size:
3066 2685 1553 768 496 370 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2280 3315 6080 1163
Size: 573 544 1035 2765 4186
Fragments arranged by size:
4186 2765 1035 573 544
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6553 7314 8342 1540
Size: 275 186 4552 761 1028 2301
Fragments arranged by size:
4552 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2845 2890 2962 2980 3142 3223 3277 3538 3772
Size: 45 72 18 162 81 54 261 234
Cuts at: 3772 4006 4015 4447 4636 4744 4834 4870 5230
Size: 234 9 432 189 108 90 36 360
Cuts at: 5230 5257 7164 7249 8320 2845
Size: 27 1907 85 1071 3628
Fragments arranged by size:
3628 1907 1071 432 360 261 234 234
189 162 108 90 85 81 72 54
45 36 27 18 9
BsmI GAATG_Cn'
Cuts at: 3210 4188 6068 6614 6707 3210
Size: 978 1880 546 93 5606
Fragments arranged by size:
5606 1880 978 546 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2845 2890 2917 2962 2980 3097 3142
Size: 399 1055 45 27 45 18 117 45
Cuts at: 3142 3223 3241 3277 3333 3466 3538 3555 3772
Size: 81 18 36 56 133 72 17 217
Cuts at: 3772 4006 4015 4042 4078 4357 4447 4492 4636
Size: 234 9 27 36 279 90 45 144
Cuts at: 4636 4716 4744 4816 4823 4834 4870 5131 5230
Size: 80 28 72 7 11 36 261 99
Cuts at: 5230 5257 5356 5534 5581 6422 7164 7249 8320
Size: 27 99 178 47 841 742 85 1071
Cuts at: 8320 1391
Size: 2174
Fragments arranged by size:
2174 1071 1055 841 742 399 279 261
234 217 178 144 133 117 99 99
90 85 81 80 72 72 56 47
45 45 45 45 36 36 36 28
27 27 27 18 18 17 11 9
7
BspEI T'CCGG_A
Cuts at: 8766 8766
Size: 9103
BspHI T'CATG_A
Cuts at: 6992 8722 6992
Size: 1730 7373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2945 3076 3287 3490 4435 4561 5416 6458
Size: 2101 131 211 203 945 126 855 1042
Cuts at: 6458 844
Size: 3489
Fragments arranged by size:
3489 2101 1042 945 855 211 203 131
126
BsrBI CCG'CTC
Cuts at: 1394 1823 6990 1394
Size: 429 5167 3507
Fragments arranged by size:
5167 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5616 6129 7598 7772 65
Size: 1183 4368 513 1469 174 1396
Fragments arranged by size:
4368 1469 1396 1183 513 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3260 3998 7747 7934 1700
Size: 110 1450 738 3749 187 2869
Fragments arranged by size:
3749 2869 1450 738 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9103
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9103
BssSI C'ACGA_G
Cuts at: 4383 4635 7163 8175 4383
Size: 252 2528 1012 5311
Fragments arranged by size:
5311 2528 1012 252
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4183 794
Size: 3389 5714
BstBI TT'CG_AA
Cuts at: 7907 7907
Size: 9103
BstEII G'GTnAC_C
Cuts at: 1184 2366 4667 1184
Size: 1182 2301 5620
Fragments arranged by size:
5620 2301 1182
BstYI r'GATC_y
Cuts at: 1972 2177 2332 2588 3038 5081 5522 6433 6800
Size: 205 155 256 450 2043 441 911 367
Cuts at: 6800 7185 7202 8654 8740 8752 9098 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2043 1977 1452 911 450 441 385 367
346 256 205 155 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3123 3816 3852 4923 3123
Size: 693 36 1071 7303
Fragments arranged by size:
7303 1071 693 36
BtgI C'CryG_G
Cuts at: 513 1065 2898 5936 5997 513
Size: 552 1833 3038 61 3619
Fragments arranged by size:
3619 3038 1833 552 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2205 487
Size: 683 56 979 7385
Fragments arranged by size:
7385 979 683 56
BtsI GCAGTG_nn'
Cuts at: 2992 6115 6619 7413 7433 2992
Size: 3123 504 794 20 4662
Fragments arranged by size:
4662 3123 794 504 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9068
DraI TTT'AAA
Cuts at: 6407 6407
Size: 9103
DraIII CAC_nnn'GTG
Cuts at: 3344 4154 5279 3344
Size: 810 1125 7168
Fragments arranged by size:
7168 1125 810
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2901 3909 4557 4683 4998 5475 8110
Size: 507 1585 1008 648 126 315 477 2635
Cuts at: 8110 809
Size: 1802
Fragments arranged by size:
2635 1802 1585 1008 648 507 477 315
126
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2933 4324 4783
Size: 54 1425 50 275 18 1103 1391 459
Cuts at: 4783 6550 7439 9068 8
Size: 1767 889 1629 43
Fragments arranged by size:
1767 1629 1425 1391 1103 889 459 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6550 1537
Size: 275 4738 4090
Fragments arranged by size:
4738 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5032 7033 1353
Size: 3679 2001 3423
Fragments arranged by size:
3679 3423 2001
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7690 8064 8210 387
Size: 1064 138 6101 374 146 1280
Fragments arranged by size:
6101 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2888 3221 4013 4832 2888
Size: 333 792 819 7159
Fragments arranged by size:
7159 819 792 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2511 2577 2622 2631 2730 2742 3234
Size: 393 879 66 45 9 99 12 492
Cuts at: 3234 3237 3435 3522 3543 3588 3615 3822 3936
Size: 3 198 87 21 45 27 207 114
Cuts at: 3936 3999 4029 4209 4353 4404 4542 4560 4683
Size: 63 30 180 144 51 138 18 123
Cuts at: 4683 4776 4818 4884 5349 6063 6070 6194 6231
Size: 93 42 66 465 714 7 124 37
Cuts at: 6231 6263 7166 7763 8550 8818 1239
Size: 32 903 597 787 268 1524
Fragments arranged by size:
1524 903 879 787 714 597 492 465
393 268 207 198 180 144 138 124
123 114 99 93 87 66 66 63
51 45 45 42 37 32 30 27
21 18 12 9 7 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3939 5850 3939
Size: 1911 7192
EcoO109I rG'GnC_Cy
Cuts at: 2312 2321 2339 2394 2543 2606 2642 2651 3002
Size: 9 18 55 149 63 36 9 351
Cuts at: 3002 3146 3200 3245 3479 3515 3542 3551 3659
Size: 144 54 45 234 36 27 9 108
Cuts at: 3659 3695 3806 3821 3857 3866 3902 4074 4075
Size: 36 111 15 36 9 36 172 1
Cuts at: 4075 4289 4424 4514 4532 4676 4685 4713 4721
Size: 214 135 90 18 144 9 28 8
Cuts at: 4721 4819 4901 5369 5405 5414 5423 5477 5495
Size: 98 82 468 36 9 9 54 18
Cuts at: 5495 5558 6169 2312
Size: 63 611 5246
Fragments arranged by size:
5246 611 468 351 234 214 172 149
144 144 135 111 108 98 90 82
63 63 55 54 54 45 36 36
36 36 36 36 28 27 18 18
18 15 9 9 9 9 9 9
9 8 1
EcoRI G'AATT_C
Cuts at: 6200 6412 6790 6200
Size: 212 378 8513
Fragments arranged by size:
8513 378 212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6068 6068
Size: 9103
FspI TGC'GCA
Cuts at: 7609 7609
Size: 9103
HaeII r_GCGC'y
Cuts at: 1678 5139 8250 1678
Size: 3461 3111 2531
Fragments arranged by size:
3461 3111 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2162 2169 2194 2201 2471
Size: 32 135 32 590 7 25 7 270
Cuts at: 2471 2503 2684 2716 3573 3605 4787 4819 7744
Size: 32 181 32 857 32 1182 32 2925
Cuts at: 7744 7776 7818 7850 1373
Size: 32 42 32 2626
Fragments arranged by size:
2925 2626 1182 857 590 270 181 135
42 32 32 32 32 32 32 32
32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2996 3689 5842 6447 6693 669
Size: 988 281 1058 693 2153 605 246 3079
Fragments arranged by size:
3079 2153 1058 988 693 605 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9103
HpaI GTT'AAC
Cuts at: 6693 6693
Size: 9103
KpnI G_GTAC'C
Cuts at: 6428 6428
Size: 9103
MfeI C'AATT_G
Cuts at: 6702 6702
Size: 9103
MluI A'CGCG_T
Cuts at: 8979 8979
Size: 9103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2303 3043 5410 5584 8217 8401 1305
Size: 998 740 2367 174 2633 184 2007
Fragments arranged by size:
2633 2367 2007 998 740 184 174
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4326 4785 10
Size: 54 1425 343 2494 459 4328
Fragments arranged by size:
4328 2494 1425 459 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3135 7061 7420 7579 518
Size: 648 1969 3926 359 159 2042
Fragments arranged by size:
3926 2042 1969 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2531 2765 3206 4322 4692 5024 5177 5612
Size: 925 234 441 1116 370 332 153 435
Cuts at: 5612 6064 6519 7196 8344 8589 1606
Size: 452 455 677 1148 245 2120
Fragments arranged by size:
2120 1148 1116 925 677 455 452 441
435 370 332 245 234 153
NcoI C'CATG_G
Cuts at: 513 1065 2898 5997 513
Size: 552 1833 3099 3619
Fragments arranged by size:
3619 3099 1833 552
NdeI CA'TA_TG
Cuts at: 387 2476 387
Size: 2089 7014
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9103
NotI GC'GGCC_GC
Cuts at: 6550 6550
Size: 9103
NsiI A_TGCA'T
Cuts at: 5912 5912
Size: 9103
NspI r_CATG'y
Cuts at: 1132 1229 3619 4897 5910 6461 8006 1132
Size: 97 2390 1278 1013 551 1545 2229
Fragments arranged by size:
2390 2229 1545 1278 1013 551 97
PasI CC'CwG_GG
Cuts at: 1348 3467 3863 4268 4816 4996 5546 1348
Size: 2119 396 405 548 180 550 4905
Fragments arranged by size:
4905 2119 550 548 405 396 180
PciI A'CATG_T
Cuts at: 8002 8002
Size: 9103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9103
PfoI T'CCnGG_A
Cuts at: 2380 2521 2593 2647 2845 2890 3016 3214 3223
Size: 141 72 54 198 45 126 198 9
Cuts at: 3223 3250 3277 3376 3439 3601 3628 3664 3826
Size: 27 27 99 63 162 27 36 162
Cuts at: 3826 3952 4015 4060 4420 4447 4519 5401 5563
Size: 126 63 45 360 27 72 882 162
Cuts at: 5563 2380
Size: 5920
Fragments arranged by size:
5920 882 360 198 198 162 162 162
141 126 126 99 72 72 63 63
54 45 45 36 27 27 27 27
9
PmeI GTTT'AAAC
Cuts at: 6407 6407
Size: 9103
PmlI CAC'GTG
Cuts at: 3341 5276 3341
Size: 1935 7168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7172 7204 8685 8717 7172
Size: 32 1481 32 7558
Fragments arranged by size:
7558 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2312 2394 2606 2651 3146 3200 3245 3479 3515
Size: 82 212 45 495 54 45 234 36
Cuts at: 3515 3695 3806 3821 3857 3902 4289 4424 4532
Size: 180 111 15 36 45 387 135 108
Cuts at: 4532 4676 4685 4721 4901 5405 5423 5477 2312
Size: 144 9 36 180 504 18 54 5938
Fragments arranged by size:
5938 504 495 387 234 212 180 180
144 135 111 108 82 54 54 45
45 45 36 36 36 18 15 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9103
PsiI TTA'TAA
Cuts at: 6673 6673
Size: 9103
PspOMI G'GGCC_C
Cuts at: 3551 4074 4712 4819 3551
Size: 523 638 107 7835
Fragments arranged by size:
7835 638 523 107
PspXI vC'TCGA_Gb
Cuts at: 2979 5499 2979
Size: 2520 6583
PstI C_TGCA'G
Cuts at: 830 2956 3073 3451 3550 5413 5792 6455 830
Size: 2126 117 378 99 1863 379 663 3478
Fragments arranged by size:
3478 2126 1863 663 379 378 117 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9103
PvuII CAG'CTG
Cuts at: 2531 2765 4322 5024 5177 6064 2531
Size: 234 1557 702 153 887 5570
Fragments arranged by size:
5570 1557 887 702 234 153
SacI G_AGCT'C
Cuts at: 2890 3223 4015 4834 2890
Size: 333 792 819 7159
Fragments arranged by size:
7159 819 792 333
SalI G'TCGA_C
Cuts at: 1936 3687 6445 1936
Size: 1751 2758 4594
Fragments arranged by size:
4594 2758 1751
SanDI GG'GwC_CC
Cuts at: 2394 3200 3857 2394
Size: 806 657 7640
Fragments arranged by size:
7640 806 657
SbfI CC_TGCA'GG
Cuts at: 3073 3550 5413 6455 3073
Size: 477 1863 1042 5721
Fragments arranged by size:
5721 1863 1042 477
ScaI AGT'ACT
Cuts at: 7351 9063 7351
Size: 1712 7391
SexAI A'CCwGG_T
Cuts at: 3421 3691 3979 4150 4465 4609 5158 3421
Size: 270 288 171 315 144 549 7366
Fragments arranged by size:
7366 549 315 288 270 171 144
SfcI C'TryA_G
Cuts at: 826 1045 2664 2952 3069 3447 3546 5409 5788
Size: 219 1619 288 117 378 99 1863 379
Cuts at: 5788 6451 7586 8267 8458 826
Size: 663 1135 681 191 1471
Fragments arranged by size:
1863 1619 1471 1135 681 663 379 378
288 219 191 117 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 2541 3874 4323 5214 2541
Size: 1333 449 891 6430
Fragments arranged by size:
6430 1333 891 449
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9103
SmaI CCC'GGG
Cuts at: 1872 3242 6430 1872
Size: 1370 3188 4545
Fragments arranged by size:
4545 3188 1370
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2316 2979 3141 5265 5499 5717
Size: 197 926 373 663 162 2124 234 218
Cuts at: 5717 5819 7207 8108 8370 820
Size: 102 1388 901 262 1553
Fragments arranged by size:
2124 1553 1388 926 901 663 373 262
234 218 197 162 102
SnaBI TAC'GTA
Cuts at: 493 6894 493
Size: 6401 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9103
SphI G_CATG'C
Cuts at: 1132 3619 4897 6461 1132
Size: 2487 1278 1564 3774
Fragments arranged by size:
3774 2487 1564 1278
SspI AAT'ATT
Cuts at: 5 52 7027 5
Size: 47 6975 2081
Fragments arranged by size:
6975 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9103
StyI C'CwwG_G
Cuts at: 513 1065 2325 2602 2898 4283 4573 4904 5851
Size: 552 1260 277 296 1385 290 331 947
Cuts at: 5851 5997 6542 513
Size: 146 545 3074
Fragments arranged by size:
3074 1385 1260 947 552 545 331 296
290 277 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5159 5559 7130 7147 7300 7485 7930
Size: 1255 3579 400 1571 17 153 185 445
Cuts at: 7930 325
Size: 1498
Fragments arranged by size:
3579 1571 1498 1255 445 400 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7349 9061 96
Size: 274 80 33 51 1443 5372 1712 138
Fragments arranged by size:
5372 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2350 2382 4726 4758 5617 5649 9063 9095 2350
Size: 32 2344 32 859 32 3414 32 2358
Fragments arranged by size:
3414 2358 2344 859 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3693 1316
Size: 2377 6726
XbaI T'CTAG_A
Cuts at: 6439 6439
Size: 9103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 2609 5003 5943 2065
Size: 544 2394 940 5225
Fragments arranged by size:
5225 2394 940 544
XhoI C'TCGA_G
Cuts at: 1943 2979 5499 1943
Size: 1036 2520 5547
Fragments arranged by size:
5547 2520 1036
XmaI C'CCGG_G
Cuts at: 1870 3240 6428 1870
Size: 1370 3188 4545
Fragments arranged by size:
4545 3188 1370
XmnI GAAnn'nnTTC
Cuts at: 7232 7232
Size: 9103
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6911 276
Size: 53 83 186 936 5377 2468
Fragments arranged by size:
5377 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BplI BsiWI
BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PsrI RsrII SacII SapI
SfoI SrfI SwaI