Restriction map for
pFN21AB3951
(Circular) (Six-base) MAPSORT of: pFN21AB3951.seq Check: 4098 from: 1 to: 5620
pFN21AB3951 5620 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2253 2353 2963 1937
Size: 316 100 610 4594
Fragments arranged by size:
4594 610 316 100
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2622 3683 5067 5335 1632
Size: 464 526 1061 1384 268 1917
Fragments arranged by size:
1917 1384 1061 526 464 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 2881 4519 5496 1203
Size: 1678 1638 977 1327
Fragments arranged by size:
1678 1638 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2075 1184
Size: 891 4729
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2199 2231 2199
Size: 32 5588
AlwNI CAG_nnn'CTG
Cuts at: 2600 3363 4935 2600
Size: 763 1572 3285
Fragments arranged by size:
3285 1572 763
ApaLI G'TGCA_C
Cuts at: 2723 3677 4833 2723
Size: 954 1156 3510
Fragments arranged by size:
3510 1156 954
ApoI r'AATT_y
Cuts at: 1493 2529 2556 2738 2929 3155 3307 5416 1493
Size: 1036 27 182 191 226 152 2109 1697
Fragments arranged by size:
2109 1697 1036 226 191 182 152 27
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2631 2945 1870
Size: 73 688 314 4545
Fragments arranged by size:
4545 688 314 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2941 4396 618
Size: 325 217 627 221 933 1455 1842
Fragments arranged by size:
1842 1455 933 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2939 1391
Size: 1548 4072
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 5620
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2815 928
Size: 971 916 3733
Fragments arranged by size:
3733 971 916
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2605 3512 4722 2605
Size: 907 1210 3503
Fragments arranged by size:
3503 1210 907
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 2727 3681 4837 1790
Size: 937 954 1156 2573
Fragments arranged by size:
2573 1156 954 937
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2226 2440 4280 1239
Size: 987 214 1840 2579
Fragments arranged by size:
2579 1840 987 214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3745 4610 4908 3745
Size: 865 298 4457
Fragments arranged by size:
4457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2270 4283 882
Size: 617 771 2013 2219
Fragments arranged by size:
2219 2013 771 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3427 3809 4444
Size: 53 83 186 936 476 1418 382 635
Cuts at: 4444 275
Size: 1451
Fragments arranged by size:
1451 1418 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2199 2229 2413 2443 2646
Size: 30 165 30 796 30 184 30 203
Cuts at: 2646 2676 1178
Size: 30 4122
Fragments arranged by size:
4122 796 203 184 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2649 1186
Size: 910 553 4157
Fragments arranged by size:
4157 910 553
BseYI C'CCAG_C
Cuts at: 4823 5319 4823
Size: 496 5124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2713 2727 3681 3766 4837 2713
Size: 14 954 85 1071 3496
Fragments arranged by size:
3496 1071 954 85 14
BsmI GAATG_Cn'
Cuts at: 3131 3224 3131
Size: 93 5527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2713 2727 2939 3681 3766 4837 1391
Size: 399 923 14 212 742 85 1071 2174
Fragments arranged by size:
2174 1071 923 742 399 212 85 14
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 2344 3509 5239 2344
Size: 1165 1730 2725
Fragments arranged by size:
2725 1730 1165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 2630 2975 844
Size: 1223 563 345 3489
Fragments arranged by size:
3489 1223 563 345
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4115 4289 65
Size: 1183 2867 174 1396
Fragments arranged by size:
2867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 2533 4424 2533
Size: 1891 3729
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2596 2950 3317 3702 3719 5171 5257 5269
Size: 624 354 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 624 385 367 354 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2167 2491 513
Size: 552 939 163 324 3642
Fragments arranged by size:
3642 939 552 324 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2770 4627 809
Size: 507 1454 1857 1802
Fragments arranged by size:
1857 1802 1454 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3067 3956 5585
Size: 54 1425 50 275 18 1237 889 1629
Cuts at: 5585 8
Size: 43
Fragments arranged by size:
1629 1425 1237 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 3550 1353
Size: 756 1441 3423
Fragments arranged by size:
3423 1441 756
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2226 2440 2622 3683 4280 5067
Size: 393 464 130 214 182 1061 597 787
Cuts at: 5067 5335 1239
Size: 268 1524
Fragments arranged by size:
1524 1061 787 597 464 393 268 214
182 130
EcoNI CCTnn'n_nnAGG
Cuts at: 2565 2565
Size: 5620
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
EcoRV GAT'ATC
Cuts at: 2164 2512 2164
Size: 348 5272
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 2012 4767 1678
Size: 334 2755 2531
Fragments arranged by size:
2755 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2646 2678 4261 4293 4335
Size: 32 135 32 1074 32 1583 32 42
Cuts at: 4335 4367 1373
Size: 32 2626
Fragments arranged by size:
2626 1583 1074 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4734 4918 1305
Size: 3429 184 2007
Fragments arranged by size:
3429 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3798
Fragments arranged by size:
3798 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 3578 3937 4096 518
Size: 648 1071 1341 359 159 2042
Fragments arranged by size:
2042 1341 1071 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3036 3713 4861 5106 1606
Size: 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 245
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 5620
NcoI C'CATG_G
Cuts at: 513 1065 2004 2167 2491 513
Size: 552 939 163 324 3642
Fragments arranged by size:
3642 939 552 324 163
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2449 2978 4523 1132
Size: 97 1220 529 1545 2229
Fragments arranged by size:
2229 1545 1220 529 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5620
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2199 2231 3689 3721 5202 5234 2199
Size: 32 1458 32 1481 32 2585
Fragments arranged by size:
2585 1481 1458 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2708 2972 830
Size: 1878 264 3478
Fragments arranged by size:
3478 1878 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2266 2704 2968 4103 4784 4975 826
Size: 219 1221 438 264 1135 681 191 1471
Fragments arranged by size:
1471 1221 1135 681 438 264 219 191
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 5620
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3724 4625 4887 820
Size: 197 926 1781 901 262 1553
Fragments arranged by size:
1781 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 2719 152
Size: 2567 3053
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 2806 3544 5
Size: 47 2754 738 2081
Fragments arranged by size:
2754 2081 738 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2004 2167 2491 3059 513
Size: 552 939 163 324 568 3074
Fragments arranged by size:
3074 939 568 552 324 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 3647 3664 3817 4002 4447 325
Size: 1255 651 1416 17 153 185 445 1498
Fragments arranged by size:
1498 1416 1255 651 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3866 5578 96
Size: 274 80 33 51 1443 1889 1712 138
Fragments arranged by size:
1889 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2399 2399
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI