Restriction map for
pFN21AB3929
(Circular) (Six-base) MAPSORT of: pFN21AB3929.seq Check: 2039 from: 1 to: 5479
pFN21AB3929 5479 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2672 3542 4926 5194 1632
Size: 1040 870 1384 268 1917
Fragments arranged by size:
1917 1384 1040 870 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 4378 5355 1203
Size: 3175 977 1327
Fragments arranged by size:
3175 1327 977
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5479
AlwNI CAG_nnn'CTG
Cuts at: 2528 3222 4794 2528
Size: 694 1572 3213
Fragments arranged by size:
3213 1572 694
ApaI G_GGCC'C
Cuts at: 2109 2109
Size: 5479
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2788 3014 3166 5275 1493
Size: 1295 226 152 2109 1697
Fragments arranged by size:
2109 1697 1295 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 2804 1870
Size: 73 145 716 4545
Fragments arranged by size:
4545 716 145 73
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2411 2800 4255 618
Size: 325 217 627 273 351 389 1455 1842
Fragments arranged by size:
1842 1455 627 389 351 325 273 217
BanII G_rGCy'C
Cuts at: 1391 2109 2247 2798 1391
Size: 718 138 551 4072
Fragments arranged by size:
4072 718 551 138
BbeI G_GCGC'C
Cuts at: 2064 2064
Size: 5479
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4508
BbvCI CC'TCA_GC
Cuts at: 2163 2163
Size: 5479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3670 3704 1903
Size: 34 1733 34 3678
Fragments arranged by size:
3678 1733 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 4090 136
Size: 107 122 71 631 605 397 2021 1525
Fragments arranged by size:
2021 1525 631 605 397 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2896 2896
Size: 5479
Bme1580I G_kGCm'C
Cuts at: 1790 2109 3540 4696 1790
Size: 319 1431 1156 2573
Fragments arranged by size:
2573 1431 1156 319
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2651 3210 4168
Size: 416 53 83 399 24 1234 559 958
Cuts at: 4168 442
Size: 1753
Fragments arranged by size:
1753 1234 958 559 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 4139 1239
Size: 1049 1851 2579
Fragments arranged by size:
2579 1851 1049
Bpu10I CC'TnA_GC
Cuts at: 2163 2163
Size: 5479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2085 2384 4142 882
Size: 617 586 299 1758 2219
Fragments arranged by size:
2219 1758 617 586 299
BsaAI yAC'GTr
Cuts at: 493 1204 3270 493
Size: 711 2066 2702
Fragments arranged by size:
2702 2066 711
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2085 3286 3668
Size: 53 83 186 936 528 24 1201 382
Cuts at: 3668 4303 275
Size: 635 1451
Fragments arranged by size:
1451 1201 936 635 528 382 186 83
53 24
BsaWI w'CCGG_w
Cuts at: 3912 4310 4584 4731 5142 3912
Size: 398 274 147 411 4249
Fragments arranged by size:
4249 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5254
Fragments arranged by size:
5254 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2274 2702 1186
Size: 1069 19 428 3963
Fragments arranged by size:
3963 1069 428 19
BseYI C'CCAG_C
Cuts at: 2424 4682 5178 2424
Size: 2258 496 2725
Fragments arranged by size:
2725 2258 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4906
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2069 2175 2929 3690 4718 1540
Size: 275 186 68 106 754 761 1028 2301
Fragments arranged by size:
2301 1028 761 754 275 186 106 68
BsiHKAI G_wGCw'C
Cuts at: 2247 3540 3625 4696 2247
Size: 1293 85 1071 3030
Fragments arranged by size:
3030 1293 1071 85
BsmI GAATG_Cn'
Cuts at: 2990 3083 2990
Size: 93 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2247 2798 3540 3625 4696 1391
Size: 399 319 138 551 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 551 399 319 138 85
BspEI T'CCGG_A
Cuts at: 5142 5142
Size: 5479
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 2834 844
Size: 1334 656 3489
Fragments arranged by size:
3489 1334 656
BsrBI CCG'CTC
Cuts at: 1394 1823 3366 1394
Size: 429 1543 3507
Fragments arranged by size:
3507 1543 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3974 4148 65
Size: 1183 2726 174 1396
Fragments arranged by size:
2726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2068 2709 4123 4310 1700
Size: 110 258 641 1414 187 2869
Fragments arranged by size:
2869 1414 641 258 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5479
BssSI C'ACGA_G
Cuts at: 3539 4551 3539
Size: 1012 4467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2137 794
Size: 1343 4136
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5479
BstXI CCAn_nnnn'nTGG
Cuts at: 2641 2641
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474
Size: 837 367 385 17 1452 86 12 346
Cuts at: 5474 1972
Size: 1977
Fragments arranged by size:
1977 1452 837 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2118 2169 2634 513
Size: 552 1053 51 465 3358
Fragments arranged by size:
3358 1053 552 465 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2995 3789 3809 2995
Size: 794 20 4665
Fragments arranged by size:
4665 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2706 2741 529
Size: 35 2142 35 3267
Fragments arranged by size:
3267 2142 35 35
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2646 4486 809
Size: 507 1330 1840 1802
Fragments arranged by size:
1840 1802 1330 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2066 2172 2926
Size: 54 1425 50 275 18 236 106 754
Cuts at: 2926 3815 5444 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 754 275 236 106 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2066 2172 2926 1537
Size: 275 254 106 754 4090
Fragments arranged by size:
4090 754 275 254 106
EarI CTCTTCn'nnn_
Cuts at: 1353 2239 3409 1353
Size: 886 1170 3423
Fragments arranged by size:
3423 1170 886
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 4440 4586 387
Size: 1064 138 2477 374 146 1280
Fragments arranged by size:
2477 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2245 2245
Size: 5479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2672 3542 4139 4926 5194 1239
Size: 393 656 384 870 597 787 268 1524
Fragments arranged by size:
1524 870 787 656 597 393 384 268
EcoO109I rG'GnC_Cy
Cuts at: 2149 2190 2149
Size: 41 5438
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FseI GG_CCGG'CC
Cuts at: 2072 2072
Size: 5479
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 2064 4626 1678
Size: 386 2562 2531
Fragments arranged by size:
2562 2531 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2615 2647 4120 4152 4194
Size: 32 135 32 1043 32 1473 32 42
Cuts at: 4194 4226 1373
Size: 32 2626
Fragments arranged by size:
2626 1473 1043 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2743 2823 3069 669
Size: 988 281 805 80 246 3079
Fragments arranged by size:
3079 988 805 281 246 80
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KasI G'GCGC_C
Cuts at: 2060 2060
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 5355 5355
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4593 4777 1305
Size: 3288 184 2007
Fragments arranged by size:
3288 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3437 3796 3955 518
Size: 648 2271 359 159 2042
Fragments arranged by size:
2271 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2120 2895 3572 4720 4965 1606
Size: 514 775 677 1148 245 2120
Fragments arranged by size:
2120 1148 775 677 514 245
NaeI GCC'GGC
Cuts at: 2070 2070
Size: 5479
NarI GG'CG_CC
Cuts at: 2061 2061
Size: 5479
NcoI C'CATG_G
Cuts at: 513 1065 2634 513
Size: 552 1569 3358
Fragments arranged by size:
3358 1569 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NgoMIV G'CCGG_C
Cuts at: 2068 2068
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5479
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2837 4382 1132
Size: 97 1608 1545 2229
Fragments arranged by size:
2229 1608 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2665 1348
Size: 1317 4162
PciI A'CATG_T
Cuts at: 4378 4378
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5479
PfoI T'CCnGG_A
Cuts at: 2265 2265
Size: 5479
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2149 2190 2149
Size: 41 5438
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PspOMI G'GGCC_C
Cuts at: 2105 2105
Size: 5479
PstI C_TGCA'G
Cuts at: 830 2831 830
Size: 2001 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
SacI G_AGCT'C
Cuts at: 2247 2247
Size: 5479
SacII CC_GC'GG
Cuts at: 2121 2121
Size: 5479
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 2135 2560 3727 5439 2135
Size: 425 1167 1712 2175
Fragments arranged by size:
2175 1712 1167 425
SfcI C'TryA_G
Cuts at: 826 1045 2827 3962 4643 4834 826
Size: 219 1782 1135 681 191 1471
Fragments arranged by size:
1782 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2062 2062
Size: 5479
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2806 1872
Size: 934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 4484 4746 820
Size: 197 926 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5479
StyI C'CwwG_G
Cuts at: 513 1065 2075 2634 2918 513
Size: 552 1010 559 284 3074
Fragments arranged by size:
3074 1010 559 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2511 3506 3523 3676 3861 4306 325
Size: 1255 931 995 17 153 185 445 1498
Fragments arranged by size:
1498 1255 995 931 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2133 2456 2558
Size: 274 80 33 51 1443 156 323 102
Cuts at: 2558 3725 5437 96
Size: 1167 1712 138
Fragments arranged by size:
1712 1443 1167 323 274 156 138 102
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5439 5471 5439
Size: 32 5447
Tth111I GACn'n_nGTC
Cuts at: 1316 2528 2643 1316
Size: 1212 115 4152
Fragments arranged by size:
4152 1212 115
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2191 2191
Size: 5479
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2804 1870
Size: 934 4545
XmnI GAAnn'nnTTC
Cuts at: 3608 3608
Size: 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SanDI SapI SexAI SfiI SrfI SwaI