Restriction map for
pFN21AB3919
(Circular) (Six-base) MAPSORT of: pFN21AB3919.seq Check: 7940 from: 1 to: 6442
pFN21AB3919 6442 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2875 4252 278
Size: 53 83 186 936 1339 1377 2468
Fragments arranged by size:
2468 1377 1339 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3785 1937
Size: 1848 4594
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 2775 3126 3159 3527 4505 5889 6157
Size: 605 538 351 33 368 978 1384 268
Cuts at: 6157 1632
Size: 1917
Fragments arranged by size:
1917 1384 978 605 538 368 351 268
33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6245
AflIII A'CryG_T
Cuts at: 1203 2121 3008 5341 6318 1203
Size: 918 887 2333 977 1327
Fragments arranged by size:
2333 1327 977 918 887
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 5171 5171
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6442
AlwNI CAG_nnn'CTG
Cuts at: 4185 5757 4185
Size: 1572 4870
ApaI G_GGCC'C
Cuts at: 2155 3171 2155
Size: 1016 5426
ApaLI G'TGCA_C
Cuts at: 4499 5655 4499
Size: 1156 5286
ApoI r'AATT_y
Cuts at: 1493 2440 2819 3411 3547 3660 3669 3751 3977
Size: 947 379 592 136 113 9 82 226
Cuts at: 3977 4129 6238 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 947 592 379 226 152 136
113 82 9
AseI AT'TA_AT
Cuts at: 160 1032 4996 160
Size: 872 3964 1606
Fragments arranged by size:
3964 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 2508 3767 1870
Size: 73 565 1259 4545
Fragments arranged by size:
4545 1259 565 73
BamHI G'GATC_C
Cuts at: 2561 3772 4139 2561
Size: 1211 367 4864
Fragments arranged by size:
4864 1211 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2648 2759 3257 3763 5218
Size: 325 217 627 861 111 498 506 1455
Cuts at: 5218 618
Size: 1842
Fragments arranged by size:
1842 1455 861 627 506 498 325 217
111
BanII G_rGCy'C
Cuts at: 1391 2155 2540 3171 3761 1391
Size: 764 385 631 590 4072
Fragments arranged by size:
4072 764 631 590 385
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2117 928
Size: 971 218 5253
Fragments arranged by size:
5253 971 218
BbvCI CC'TCA_GC
Cuts at: 2030 3021 2030
Size: 991 5451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2225 2259 3281 3315 4633 4667 1903
Size: 34 288 34 1022 34 1318 34 3678
Fragments arranged by size:
3678 1318 1022 288 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2578 4334 5544 2578
Size: 1756 1210 3476
Fragments arranged by size:
3476 1756 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6442
BfrBI ATG'CAT
Cuts at: 2797 2797
Size: 6442
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2159 2168 5053
Size: 107 122 71 631 605 487 9 2885
Cuts at: 5053 136
Size: 1525
Fragments arranged by size:
2885 1525 631 605 487 122 107 71
9
BglII A'GATC_T
Cuts at: 6437 6437
Size: 6442
BlpI GC'TnA_GC
Cuts at: 3859 3859
Size: 6442
Bme1580I G_kGCm'C
Cuts at: 1790 2155 3171 4503 5659 1790
Size: 365 1016 1332 1156 2573
Fragments arranged by size:
2573 1332 1156 1016 365
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6442
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2674 4173 5131
Size: 416 53 83 399 24 1257 1499 958
Cuts at: 5131 442
Size: 1753
Fragments arranged by size:
1753 1499 1257 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2541 2807 3315 3330 5102 1239
Size: 1302 266 508 15 1772 2579
Fragments arranged by size:
2579 1772 1302 508 266 15
Bpu10I CC'TnA_GC
Cuts at: 2030 3021 3402 2030
Size: 991 381 5070
Fragments arranged by size:
5070 991 381
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 2527 2793 4567 5432 5730 2141
Size: 386 266 1774 865 298 2853
Fragments arranged by size:
2853 1774 865 386 298 266
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2559 5105 882
Size: 617 1060 2546 2219
Fragments arranged by size:
2546 2219 1060 617
BsaAI yAC'GTr
Cuts at: 493 1204 4233 493
Size: 711 3029 2702
Fragments arranged by size:
3029 2702 711
BsaBI GATnn'nnATC
Cuts at: 2566 2738 6436 2566
Size: 172 3698 2572
Fragments arranged by size:
3698 2572 172
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 2872 4249 4631
Size: 53 83 186 936 543 796 1377 382
Cuts at: 4631 5266 275
Size: 635 1451
Fragments arranged by size:
1451 1377 936 796 635 543 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4875 5273 5547 5694 6105 4875
Size: 398 274 147 411 5212
Fragments arranged by size:
5212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2926 2956 1178
Size: 30 165 30 1523 30 4664
Fragments arranged by size:
4664 1523 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2530 3038 3041 3177 1186
Size: 1344 508 3 136 4451
Fragments arranged by size:
4451 1344 508 136 3
BseYI C'CCAG_C
Cuts at: 2148 2484 3241 5645 6141 2148
Size: 336 757 2404 496 2449
Fragments arranged by size:
2449 2404 757 496 336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 3579 1163
Size: 573 441 1402 4026
Fragments arranged by size:
4026 1402 573 441
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3892 4653 5681 1540
Size: 275 186 1891 761 1028 2301
Fragments arranged by size:
2301 1891 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 4503 4588 5659 2220
Size: 2283 85 1071 3003
Fragments arranged by size:
3003 2283 1071 85
BsmI GAATG_Cn'
Cuts at: 3953 4046 3953
Size: 93 6349
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2155 2220 2540 3171 3761 4503 4588
Size: 399 365 65 320 631 590 742 85
Cuts at: 4588 5659 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 631 590 399 365 320
85 65
BspEI T'CCGG_A
Cuts at: 6105 6105
Size: 6442
BspHI T'CATG_A
Cuts at: 3033 4331 6061 3033
Size: 1298 1730 3414
Fragments arranged by size:
3414 1730 1298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3797 844
Size: 2953 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4329 1394
Size: 429 2506 3507
Fragments arranged by size:
3507 2506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2945 3004 4937 5111 65
Size: 1183 1697 59 1933 174 1396
Fragments arranged by size:
1933 1697 1396 1183 174 59
BsrFI r'CCGG_y
Cuts at: 1700 1810 5086 5273 1700
Size: 110 3276 187 2869
Fragments arranged by size:
3276 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6442
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 3576 4502 5514 3576
Size: 926 1012 4504
Fragments arranged by size:
4504 1012 926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3347 794
Size: 2553 3889
BstBI TT'CG_AA
Cuts at: 5246 5246
Size: 6442
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6442
BstXI CCAn_nnnn'nTGG
Cuts at: 2624 2624
Size: 6442
BstYI r'GATC_y
Cuts at: 1972 2082 2561 2832 3772 4139 4524 4541 5993
Size: 110 479 271 940 367 385 17 1452
Cuts at: 5993 6079 6091 6437 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 940 479 385 367 346 271
110 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3163 3163
Size: 6442
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5890
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5703
Fragments arranged by size:
5703 683 56
BtsI GCAGTG_nn'
Cuts at: 3958 4752 4772 3958
Size: 794 20 5628
Fragments arranged by size:
5628 794 20
ClaI AT'CG_AT
Cuts at: 1554 2691 1554
Size: 1137 5305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2602 2637 529
Size: 35 2038 35 4334
Fragments arranged by size:
4334 2038 35 35
DraI TTT'AAA
Cuts at: 3746 3746
Size: 6442
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5449 809
Size: 507 4133 1802
Fragments arranged by size:
4133 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2478 3889 4778
Size: 54 1425 50 275 18 648 1411 889
Cuts at: 4778 6407 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1411 889 648 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3889 1537
Size: 275 2077 4090
Fragments arranged by size:
4090 2077 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2823 3450 4372 1353
Size: 1470 627 922 3423
Fragments arranged by size:
3423 1470 922 627
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5029 5403 5549 387
Size: 1064 138 3440 374 146 1280
Fragments arranged by size:
3440 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2541 2775 2807 3126 3159 3315
Size: 393 605 304 234 32 319 33 156
Cuts at: 3315 3330 3527 4505 5102 5889 6157 1239
Size: 15 197 978 597 787 268 1524
Fragments arranged by size:
1524 978 787 605 597 393 319 304
268 234 197 156 33 32 15
EcoO109I rG'GnC_Cy
Cuts at: 2096 2152 3167 3168 2096
Size: 56 1015 1 5370
Fragments arranged by size:
5370 1015 56 1
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
EcoRV GAT'ATC
Cuts at: 2940 2940
Size: 6442
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 2174 5589 1678
Size: 496 3415 2531
Fragments arranged by size:
3415 2531 496
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2836 2868 3139 3171 3214
Size: 32 135 32 1264 32 271 32 43
Cuts at: 3214 3246 5083 5115 5157 5189 1373
Size: 32 1837 32 42 32 2626
Fragments arranged by size:
2626 1837 1264 271 135 43 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3786 4032 669
Size: 988 281 1848 246 3079
Fragments arranged by size:
3079 1848 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6442
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 2121 6318 2121
Size: 4197 2245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2605 2848 5556 5740 1305
Size: 1300 243 2708 184 2007
Fragments arranged by size:
2708 2007 1300 243 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4620
Fragments arranged by size:
4620 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3633 4400 4759 4918 518
Size: 648 2467 767 359 159 2042
Fragments arranged by size:
2467 2042 767 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3335 3858 4535 5683 5928 1606
Size: 1729 523 677 1148 245 2120
Fragments arranged by size:
2120 1729 1148 677 523 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5890
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6442
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NsiI A_TGCA'T
Cuts at: 2799 2799
Size: 6442
NspI r_CATG'y
Cuts at: 1132 1229 3012 3800 5345 1132
Size: 97 1783 788 1545 2229
Fragments arranged by size:
2229 1783 1545 788 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6442
PciI A'CATG_T
Cuts at: 3008 5341 3008
Size: 2333 4109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6442
PfoI T'CCnGG_A
Cuts at: 2557 3100 2557
Size: 543 5899
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2336 2368 3377 3409 4511 4543 6024 6056 2336
Size: 32 1009 32 1102 32 1481 32 2722
Fragments arranged by size:
2722 1481 1102 1009 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2096 2096
Size: 6442
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6442
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PspOMI G'GGCC_C
Cuts at: 2151 3167 2151
Size: 1016 5426
PstI C_TGCA'G
Cuts at: 830 2365 3794 830
Size: 1535 1429 3478
Fragments arranged by size:
3478 1535 1429
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SanDI GG'GwC_CC
Cuts at: 2096 2096
Size: 6442
SbfI CC_TGCA'GG
Cuts at: 3794 3794
Size: 6442
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SfcI C'TryA_G
Cuts at: 826 1045 2361 3790 4925 5606 5797 826
Size: 219 1316 1429 1135 681 191 1471
Fragments arranged by size:
1471 1429 1316 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2159 2159
Size: 6442
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 3769 1872
Size: 1897 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2156 2542 2808 4546 5447 5709
Size: 197 926 213 386 266 1738 901 262
Cuts at: 5709 820
Size: 1553
Fragments arranged by size:
1738 1553 926 901 386 266 262 213
197
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 2305 152
Size: 2153 4289
SphI G_CATG'C
Cuts at: 1132 3800 1132
Size: 2668 3774
SspI AAT'ATT
Cuts at: 5 52 4366 5
Size: 47 4314 2081
Fragments arranged by size:
4314 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6442
StyI C'CwwG_G
Cuts at: 513 1065 3120 3881 513
Size: 552 2055 761 3074
Fragments arranged by size:
3074 2055 761 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2212 2630 3151 4469 4486 4639 4824
Size: 1255 632 418 521 1318 17 153 185
Cuts at: 4824 5269 325
Size: 445 1498
Fragments arranged by size:
1498 1318 1255 632 521 445 418 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4688 6400 96
Size: 274 80 33 51 1443 2711 1712 138
Fragments arranged by size:
2711 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2926 2958 6402 6434 2926
Size: 32 3444 32 2934
Fragments arranged by size:
3444 2934 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2680 1316
Size: 1364 5078
XbaI T'CTAG_A
Cuts at: 3778 3778
Size: 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 3767 1870
Size: 1897 4545
XmnI GAAnn'nnTTC
Cuts at: 4571 4571
Size: 6442
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2873 4250 276
Size: 53 83 186 936 1339 1377 2468
Fragments arranged by size:
2468 1377 1339 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SfoI SrfI SwaI XcmI