Restriction map for
pFN21AB3906
(Circular) (Six-base) MAPSORT of: pFN21AB3906.seq Check: 6668 from: 1 to: 5959
pFN21AB3906 5959 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2086 4022 5406 5674 1632
Size: 454 1936 1384 268 1917
Fragments arranged by size:
1936 1917 1384 454 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2377 2774 1184
Size: 1193 397 4369
Fragments arranged by size:
4369 1193 397
AlwNI CAG_nnn'CTG
Cuts at: 3702 5274 3702
Size: 1572 4387
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2549 3268 3494 3646 5755 1493
Size: 1056 719 226 152 2109 1697
Fragments arranged by size:
2109 1697 1056 719 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3090 4513 160
Size: 872 2058 1423 1606
Fragments arranged by size:
2058 1606 1423 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 3284 1870
Size: 73 115 1226 4545
Fragments arranged by size:
4545 1226 115 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2016 2054 2081 3280 4735
Size: 325 217 627 229 38 27 1199 1455
Cuts at: 4735 618
Size: 1842
Fragments arranged by size:
1842 1455 1199 627 325 229 217 38
27
BanII G_rGCy'C
Cuts at: 1391 2189 2479 3278 1391
Size: 798 290 799 4072
Fragments arranged by size:
4072 799 798 290
BbeI G_GCGC'C
Cuts at: 2020 2085 2020
Size: 65 5894
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4150 4184 1903
Size: 34 50 34 2129 34 3678
Fragments arranged by size:
3678 2129 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 2403 3376 2403
Size: 973 4986
Bme1580I G_kGCm'C
Cuts at: 1790 2059 2205 4020 5176 1790
Size: 269 146 1815 1156 2573
Fragments arranged by size:
2573 1815 1156 269 146
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2137 2182 3690
Size: 416 53 83 399 24 720 45 1508
Cuts at: 3690 4648 442
Size: 958 1753
Fragments arranged by size:
1753 1508 958 720 416 399 83 53
45 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4619 1239
Size: 3380 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4949 5247 4084
Size: 865 298 4796
Fragments arranged by size:
4796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2635 3750 493
Size: 711 1431 1115 2702
Fragments arranged by size:
2702 1431 1115 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2017 2082 3766 4148
Size: 53 83 186 936 484 65 1684 382
Cuts at: 4148 4783 275
Size: 635 1451
Fragments arranged by size:
1684 1451 936 635 484 382 186 83
65 53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5734
Fragments arranged by size:
5734 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5959
BseYI C'CCAG_C
Cuts at: 5162 5658 5162
Size: 496 5463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2291 2699 2942 1163
Size: 573 417 138 408 243 4180
Fragments arranged by size:
4180 573 417 408 243 138
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2912 4020 4105 5176 2912
Size: 1108 85 1071 3695
Fragments arranged by size:
3695 1108 1071 85
BsiWI C'GTAC_G
Cuts at: 2220 2220
Size: 5959
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2189 2205 2479 2912 3278 4020
Size: 399 269 130 16 274 433 366 742
Cuts at: 4020 4105 5176 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 366 274 269
130 85 16
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 3314 844
Size: 1331 1139 3489
Fragments arranged by size:
3489 1331 1139
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2649 4454 4628 65
Size: 1183 1401 1805 174 1396
Fragments arranged by size:
1805 1401 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 2445 96
Size: 2349 3610
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954
Size: 1317 367 385 17 1452 86 12 346
Cuts at: 5954 1972
Size: 1977
Fragments arranged by size:
1977 1452 1317 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2012 2232 513
Size: 552 947 220 4240
Fragments arranged by size:
4240 947 552 220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 2272 2290 3194 3475 4269 4289 2272
Size: 18 904 281 794 20 3942
Fragments arranged by size:
3942 904 794 281 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DraIII CAC_nnn'GTG
Cuts at: 2131 2131
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2328 4966 809
Size: 507 1012 2638 1802
Fragments arranged by size:
2638 1802 1012 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3406 4295 5924
Size: 54 1425 50 275 18 1576 889 1629
Cuts at: 5924 8
Size: 43
Fragments arranged by size:
1629 1576 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 2734 3889 1353
Size: 824 557 1155 3423
Fragments arranged by size:
3423 1155 824 557
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 4022 4619 5406 5674 1239
Size: 393 454 1936 597 787 268 1524
Fragments arranged by size:
1936 1524 787 597 454 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2097 2228 2468 2097
Size: 131 240 5588
Fragments arranged by size:
5588 240 131
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
EcoRV GAT'ATC
Cuts at: 2149 2149
Size: 5959
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2020 2085 5106 1678
Size: 342 65 3021 2531
Fragments arranged by size:
3021 2531 342 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3105 3137 4600 4632 4674
Size: 32 135 32 1533 32 1463 32 42
Cuts at: 4674 4706 1373
Size: 32 2626
Fragments arranged by size:
2626 1533 1463 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KasI G'GCGC_C
Cuts at: 2016 2081 2016
Size: 65 5894
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 2913 5073 5257 1305
Size: 789 819 2160 184 2007
Fragments arranged by size:
2160 2007 819 789 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4137
Fragments arranged by size:
4137 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2756 3917 4276 4435 518
Size: 648 1590 1161 359 159 2042
Fragments arranged by size:
2042 1590 1161 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2293 3206 3375 4052 5200 5445 1606
Size: 408 279 913 169 677 1148 245 2120
Fragments arranged by size:
2120 1148 913 677 408 279 245 169
NarI GG'CG_CC
Cuts at: 2017 2082 2017
Size: 65 5894
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5407
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3203 3317 4862 1132
Size: 97 1974 114 1545 2229
Fragments arranged by size:
2229 1974 1545 114 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2097 2468 2097
Size: 371 5588
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3162 3182 3529 3162
Size: 20 347 5592
Fragments arranged by size:
5592 347 20
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2598 2630 2598
Size: 32 5927
PstI C_TGCA'G
Cuts at: 830 3311 830
Size: 2481 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2293 3206 2293
Size: 913 5046
SacII CC_GC'GG
Cuts at: 2015 2015
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SapI GCTCTTCn'nnn_
Cuts at: 2177 2177
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 3307 4442 5123 5314 826
Size: 219 2262 1135 681 191 1471
Fragments arranged by size:
2262 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2018 2083 2018
Size: 65 5894
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 2060 3286 1872
Size: 188 1226 4545
Fragments arranged by size:
4545 1226 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4063 4964 5226 820
Size: 197 926 2120 901 262 1553
Fragments arranged by size:
2120 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3203 3317 1132
Size: 2071 114 3774
Fragments arranged by size:
3774 2071 114
SspI AAT'ATT
Cuts at: 5 52 2440 3883 5
Size: 47 2388 1443 2081
Fragments arranged by size:
2388 2081 1443 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2564 3067 3398 513
Size: 552 1499 503 331 3074
Fragments arranged by size:
3074 1499 552 503 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2119 3986 4003 4156 4341 4786 325
Size: 1255 539 1867 17 153 185 445 1498
Fragments arranged by size:
1867 1498 1255 539 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2445 2498 3189
Size: 274 80 33 51 1443 468 53 691
Cuts at: 3189 4205 5917 96
Size: 1016 1712 138
Fragments arranged by size:
1712 1443 1016 691 468 274 138 80
53 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 2058 3284 1870
Size: 188 1226 4545
Fragments arranged by size:
4545 1226 188
XmnI GAAnn'nnTTC
Cuts at: 2610 3081 4088 2610
Size: 471 1007 4481
Fragments arranged by size:
4481 1007 471
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI RsrII SacI SanDI SexAI SfiI
SrfI SwaI XcmI