Restriction map for
pFN21AB3902
(Circular) (Six-base) MAPSORT of: pFN21AB3902.seq Check: 5369 from: 1 to: 6541
pFN21AB3902 6541 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3832 4351 278
Size: 53 83 186 936 2296 519 2468
Fragments arranged by size:
2468 2296 936 519 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2907 3376 3884 1937
Size: 970 469 508 4594
Fragments arranged by size:
4594 970 508 469
Acc65I G'GTAC_C
Cuts at: 3862 3862
Size: 6541
AclI AA'CG_TT
Cuts at: 1563 4668 5041 1563
Size: 3105 373 3063
Fragments arranged by size:
3105 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4604 5988 6256 1632
Size: 2972 1384 268 1917
Fragments arranged by size:
2972 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6344
AflIII A'CryG_T
Cuts at: 1203 2920 3585 5440 6417 1203
Size: 1717 665 1855 977 1327
Fragments arranged by size:
1855 1717 1327 977 665
AgeI A'CCGG_T
Cuts at: 5372 5372
Size: 6541
AhdI GACnn_n'nnGTC
Cuts at: 2454 5270 2454
Size: 2816 3725
AleI CACnn'nnGTG
Cuts at: 1166 2431 1166
Size: 1265 5276
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6541
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3672 3704 3672
Size: 32 6509
AlwNI CAG_nnn'CTG
Cuts at: 2452 3028 4284 5856 2452
Size: 576 1256 1572 3137
Fragments arranged by size:
3137 1572 1256 576
ApaI G_GGCC'C
Cuts at: 3498 3498
Size: 6541
ApaLI G'TGCA_C
Cuts at: 4598 5754 4598
Size: 1156 5385
ApoI r'AATT_y
Cuts at: 1493 3386 3850 4076 4228 6337 1493
Size: 1893 464 226 152 2109 1697
Fragments arranged by size:
2109 1893 1697 464 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5095 160
Size: 872 4063 1606
Fragments arranged by size:
4063 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
AvaI C'yCGr_G
Cuts at: 1870 1943 3866 1870
Size: 73 1923 4545
Fragments arranged by size:
4545 1923 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2197 2230 2197
Size: 33 6508
BamHI G'GATC_C
Cuts at: 3871 4238 3871
Size: 367 6174
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3862 5317 618
Size: 325 217 627 2075 1455 1842
Fragments arranged by size:
2075 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2087 2307 3201 3498 3860 1391
Size: 696 220 894 297 362 4072
Fragments arranged by size:
4072 894 696 362 297 220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2267 2450 3291 928
Size: 971 368 183 841 4178
Fragments arranged by size:
4178 971 841 368 183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4732 4766 1903
Size: 34 2795 34 3678
Fragments arranged by size:
3678 2795 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3310 4433 5643 3310
Size: 1123 1210 4208
Fragments arranged by size:
4208 1210 1123
BclI T'GATC_A
Cuts at: 1548 3360 1548
Size: 1812 4729
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5152 136
Size: 107 122 71 631 605 3480 1525
Fragments arranged by size:
3480 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6536 6536
Size: 6541
BlpI GC'TnA_GC
Cuts at: 3958 3958
Size: 6541
Bme1580I G_kGCm'C
Cuts at: 1790 3161 3498 4602 5758 1790
Size: 1371 337 1104 1156 2573
Fragments arranged by size:
2573 1371 1156 1104 337
BmgBI CAC'GTC
Cuts at: 1318 2653 2853 1318
Size: 1335 200 5006
Fragments arranged by size:
5006 1335 200
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2751 3164 3541
Size: 416 53 83 399 24 1334 413 377
Cuts at: 3541 4272 5230 442
Size: 731 958 1753
Fragments arranged by size:
1753 1334 958 731 416 413 399 377
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6541
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3448 3448
Size: 6541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2954 2975 5201 1239
Size: 1715 21 2226 2579
Fragments arranged by size:
2579 2226 1715 21
Bpu10I CC'TnA_GC
Cuts at: 2943 2943
Size: 6541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2218 3176 4666 5531 5829 2218
Size: 958 1490 865 298 2930
Fragments arranged by size:
2930 1490 958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5204 882
Size: 617 3705 2219
Fragments arranged by size:
3705 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2921 3588 4332 493
Size: 711 1717 667 744 2702
Fragments arranged by size:
2702 1717 744 711 667
BsaBI GATnn'nnATC
Cuts at: 6535 6535
Size: 6541
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3829 4348 4730 5365
Size: 53 83 186 936 2296 519 382 635
Cuts at: 5365 275
Size: 1451
Fragments arranged by size:
2296 1451 936 635 519 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2312 4974 5372 5646 5793 6204 2312
Size: 2662 398 274 147 411 2649
Fragments arranged by size:
2662 2649 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6316
Fragments arranged by size:
6316 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2951 3440 1186
Size: 1765 489 4287
Fragments arranged by size:
4287 1765 489
BseYI C'CCAG_C
Cuts at: 2023 2056 2589 5744 6240 2023
Size: 33 533 3155 496 2324
Fragments arranged by size:
3155 2324 533 496 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2987 2996 3431 1163
Size: 573 1251 9 435 4273
Fragments arranged by size:
4273 1251 573 435 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2147 3991 4752 5780 1540
Size: 275 186 146 1844 761 1028 2301
Fragments arranged by size:
2301 1844 1028 761 275 186 146
BsiHKAI G_wGCw'C
Cuts at: 2307 2961 3215 4602 4687 5758 2307
Size: 654 254 1387 85 1071 3090
Fragments arranged by size:
3090 1387 1071 654 254 85
BsiWI C'GTAC_G
Cuts at: 2665 2665
Size: 6541
BsmI GAATG_Cn'
Cuts at: 2355 4052 4145 2355
Size: 1697 93 4751
Fragments arranged by size:
4751 1697 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2307 2961 3161 3201 3215 3498
Size: 399 297 220 654 200 40 14 283
Cuts at: 3498 3860 4602 4687 5758 1391
Size: 362 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 654 399 362 297 283
220 200 85 40 14
BspEI T'CCGG_A
Cuts at: 6204 6204
Size: 6541
BspHI T'CATG_A
Cuts at: 3357 4430 6160 3357
Size: 1073 1730 3738
Fragments arranged by size:
3738 1730 1073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3896 844
Size: 3052 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4428 1394
Size: 429 2605 3507
Fragments arranged by size:
3507 2605 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2206 2704 2856 2890 3138 5036 5210
Size: 1183 958 498 152 34 248 1898 174
Cuts at: 5210 65
Size: 1396
Fragments arranged by size:
1898 1396 1183 958 498 248 174 152
34
BsrFI r'CCGG_y
Cuts at: 1700 1810 2139 2415 5185 5372 1700
Size: 110 329 276 2770 187 2869
Fragments arranged by size:
2869 2770 329 276 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6541
BssSI C'ACGA_G
Cuts at: 2431 4601 5613 2431
Size: 2170 1012 3359
Fragments arranged by size:
3359 2170 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3010 3028 794
Size: 2216 18 4307
Fragments arranged by size:
4307 2216 18
BstBI TT'CG_AA
Cuts at: 5345 5345
Size: 6541
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6541
BstYI r'GATC_y
Cuts at: 1972 3871 4238 4623 4640 6092 6178 6190 6536
Size: 1899 367 385 17 1452 86 12 346
Cuts at: 6536 1972
Size: 1977
Fragments arranged by size:
1977 1899 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3684 3684
Size: 6541
BtgI C'CryG_G
Cuts at: 513 1065 2004 2292 3405 3460 3784 513
Size: 552 939 288 1113 55 324 3270
Fragments arranged by size:
3270 1113 939 552 324 288 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5802
Fragments arranged by size:
5802 683 56
BtsI GCAGTG_nn'
Cuts at: 2959 3443 4057 4851 4871 2959
Size: 484 614 794 20 4629
Fragments arranged by size:
4629 794 614 484 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6541
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6506
DraI TTT'AAA
Cuts at: 3845 3845
Size: 6541
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2735 3506 3590 5548 809
Size: 507 1419 771 84 1958 1802
Fragments arranged by size:
1958 1802 1419 771 507 84
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2144 3181 3988
Size: 54 1425 50 275 18 314 1037 807
Cuts at: 3988 4877 6506 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1037 889 807 314 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2144 3988 1537
Size: 275 332 1844 4090
Fragments arranged by size:
4090 1844 332 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2168 2447 2606 3101 3356 4471 1353
Size: 815 279 159 495 255 1115 3423
Fragments arranged by size:
3423 1115 815 495 279 255 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2059 5128 5502 5648 387
Size: 1064 138 470 3069 374 146 1280
Fragments arranged by size:
3069 1280 1064 470 374 146 138
EcoICRI GAG'CTC
Cuts at: 2305 2305
Size: 6541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2954 2975 4604 5201 5988 6256 1239
Size: 393 1322 21 1629 597 787 268 1524
Fragments arranged by size:
1629 1524 1322 787 597 393 268 21
EcoO109I rG'GnC_Cy
Cuts at: 3401 3401
Size: 6541
EcoRI G'AATT_C
Cuts at: 3850 4228 3850
Size: 378 6163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3217 3217
Size: 6541
FspI TGC'GCA
Cuts at: 5047 5047
Size: 6541
HaeII r_GCGC'y
Cuts at: 1678 5688 1678
Size: 4010 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2440 2472 5182 5214 5256
Size: 32 135 32 868 32 2710 32 42
Cuts at: 5256 5288 1373
Size: 32 2626
Fragments arranged by size:
2710 2626 868 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2656 3377 3885 4131 669
Size: 988 281 718 721 508 246 3079
Fragments arranged by size:
3079 988 721 718 508 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6541
HpaI GTT'AAC
Cuts at: 4131 4131
Size: 6541
KpnI G_GTAC'C
Cuts at: 3866 3866
Size: 6541
MfeI C'AATT_G
Cuts at: 2134 4140 2134
Size: 2006 4535
MluI A'CGCG_T
Cuts at: 6417 6417
Size: 6541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2454 3292 5655 5839 1305
Size: 1149 838 2363 184 2007
Fragments arranged by size:
2363 2007 1149 838 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3183 10
Size: 54 1425 343 1351 3368
Fragments arranged by size:
3368 1425 1351 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2431 3050 3515 4499 4858 5017 518
Size: 648 1265 619 465 984 359 159 2042
Fragments arranged by size:
2042 1265 984 648 619 465 359 159
MspA1I CmG'CkG
Cuts at: 1606 2499 2511 3957 4634 5782 6027 1606
Size: 893 12 1446 677 1148 245 2120
Fragments arranged by size:
2120 1446 1148 893 677 245 12
NaeI GCC'GGC
Cuts at: 2141 2141
Size: 6541
NcoI C'CATG_G
Cuts at: 513 1065 2004 3784 513
Size: 552 939 1780 3270
Fragments arranged by size:
3270 1780 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6541
NgoMIV G'CCGG_C
Cuts at: 2139 2139
Size: 6541
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6541
NotI GC'GGCC_GC
Cuts at: 3988 3988
Size: 6541
NspI r_CATG'y
Cuts at: 1132 1229 3899 5444 1132
Size: 97 2670 1545 2229
Fragments arranged by size:
2670 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6541
PciI A'CATG_T
Cuts at: 5440 5440
Size: 6541
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6541
PfoI T'CCnGG_A
Cuts at: 2172 3760 2172
Size: 1588 4953
PmeI GTTT'AAAC
Cuts at: 3845 3845
Size: 6541
PmlI CAC'GTG
Cuts at: 2921 3588 2921
Size: 667 5874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4610 4642 6123 6155 4610
Size: 32 1481 32 4996
Fragments arranged by size:
4996 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3401 3401
Size: 6541
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6541
PsiI TTA'TAA
Cuts at: 4111 4111
Size: 6541
PspOMI G'GGCC_C
Cuts at: 3494 3494
Size: 6541
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 6509
PstI C_TGCA'G
Cuts at: 830 2120 2498 2751 3893 830
Size: 1290 378 253 1142 3478
Fragments arranged by size:
3478 1290 1142 378 253
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6541
PvuII CAG'CTG
Cuts at: 2499 2499
Size: 6541
SacI G_AGCT'C
Cuts at: 2307 2307
Size: 6541
SalI G'TCGA_C
Cuts at: 1936 3375 3883 1936
Size: 1439 508 4594
Fragments arranged by size:
4594 1439 508
SanDI GG'GwC_CC
Cuts at: 3401 3401
Size: 6541
SapI GCTCTTCn'nnn_
Cuts at: 2447 3101 2447
Size: 654 5887
SbfI CC_TGCA'GG
Cuts at: 3893 3893
Size: 6541
ScaI AGT'ACT
Cuts at: 3551 4789 6501 3551
Size: 1238 1712 3591
Fragments arranged by size:
3591 1712 1238
SfcI C'TryA_G
Cuts at: 826 1045 2116 2494 2747 3889 5024 5705 5896
Size: 219 1071 378 253 1142 1135 681 191
Cuts at: 5896 826
Size: 1471
Fragments arranged by size:
1471 1142 1135 1071 681 378 253 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6541
SmaI CCC'GGG
Cuts at: 1872 3868 1872
Size: 1996 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2233 3191 4645 5546 5808 820
Size: 197 926 290 958 1454 901 262 1553
Fragments arranged by size:
1553 1454 958 926 901 290 262 197
SnaBI TAC'GTA
Cuts at: 493 4332 493
Size: 3839 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6541
SphI G_CATG'C
Cuts at: 1132 3899 1132
Size: 2767 3774
SspI AAT'ATT
Cuts at: 5 52 4465 5
Size: 47 4413 2081
Fragments arranged by size:
4413 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6541
StyI C'CwwG_G
Cuts at: 513 1065 2004 3713 3784 3980 513
Size: 552 939 1709 71 196 3074
Fragments arranged by size:
3074 1709 939 552 196 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3125 4568 4585 4738 4923 5368 325
Size: 1255 1545 1443 17 153 185 445 1498
Fragments arranged by size:
1545 1498 1443 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3549 4787 6499
Size: 274 80 33 51 1443 1572 1238 1712
Cuts at: 6499 96
Size: 138
Fragments arranged by size:
1712 1572 1443 1238 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6501 6533 6501
Size: 32 6509
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6541
XbaI T'CTAG_A
Cuts at: 3877 3877
Size: 6541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3003 3119 3151 3176 3003
Size: 116 32 25 6368
Fragments arranged by size:
6368 116 32 25
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6541
XmaI C'CCGG_G
Cuts at: 1870 3866 1870
Size: 1996 4545
XmnI GAAnn'nnTTC
Cuts at: 2162 2486 4670 2162
Size: 324 2184 4033
Fragments arranged by size:
4033 2184 324
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3830 4349 276
Size: 53 83 186 936 2296 519 2468
Fragments arranged by size:
2468 2296 936 519 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BbvCI BfrBI BsmBI
BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PspXI RsrII SacII SexAI
SfiI SfoI SrfI SwaI