Restriction map for
pFN21AB3878
(Circular) (Six-base) MAPSORT of: pFN21AB3878.seq Check: 1817 from: 1 to: 5851
pFN21AB3878 5851 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3066 3066
Size: 5851
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 278
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3194 1937
Size: 1257 4594
Acc65I G'GTAC_C
Cuts at: 3172 3172
Size: 5851
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2560 3914 5298 5566 1632
Size: 928 1354 1384 268 1917
Fragments arranged by size:
1917 1384 1354 928 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5654
AflIII A'CryG_T
Cuts at: 1203 4750 5727 1203
Size: 3547 977 1327
Fragments arranged by size:
3547 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 4580 4580
Size: 5851
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5851
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2537 2569 2537
Size: 32 5819
AlwNI CAG_nnn'CTG
Cuts at: 3594 5166 3594
Size: 1572 4279
ApaI G_GGCC'C
Cuts at: 2100 2100
Size: 5851
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 3160 3386 3538 5647 1493
Size: 1667 226 152 2109 1697
Fragments arranged by size:
2109 1697 1667 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2099 3176 1870
Size: 73 144 12 1077 4545
Fragments arranged by size:
4545 1077 144 73 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2936 2969 2936
Size: 33 5818
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3172 4627 618
Size: 325 217 627 1385 1455 1842
Fragments arranged by size:
1842 1455 1385 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2100 2355 3170 1391
Size: 709 255 815 4072
Fragments arranged by size:
4072 815 709 255
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2566 2629 928
Size: 971 667 63 4150
Fragments arranged by size:
4150 971 667 63
BbvCI CC'TCA_GC
Cuts at: 2145 2531 2145
Size: 386 5465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4042 4076 1903
Size: 34 2105 34 3678
Fragments arranged by size:
3678 2105 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2777 3743 4953 2777
Size: 966 1210 3675
Fragments arranged by size:
3675 1210 966
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 4462 136
Size: 107 122 71 631 605 382 2408 1525
Fragments arranged by size:
2408 1525 631 605 382 122 107 71
BglII A'GATC_T
Cuts at: 5846 5846
Size: 5851
BlpI GC'TnA_GC
Cuts at: 2537 2624 3268 2537
Size: 87 644 5120
Fragments arranged by size:
5120 644 87
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2925 3912 5068 1790
Size: 310 825 987 1156 2573
Fragments arranged by size:
2573 1156 987 825 310
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2103 3582 4540
Size: 416 53 83 399 24 686 1479 958
Cuts at: 4540 442
Size: 1753
Fragments arranged by size:
1753 1479 958 686 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2178 2315 2462 2539 2543 2594 2606 3062
Size: 939 137 147 77 4 51 12 456
Cuts at: 3062 4511 1239
Size: 1449 2579
Fragments arranged by size:
2579 1449 939 456 147 137 77 51
12 4
Bpu10I CC'TnA_GC
Cuts at: 2145 2531 2145
Size: 386 5465
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2648 3976 4841 5139 2648
Size: 1328 865 298 3360
Fragments arranged by size:
3360 1328 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2762 4514 882
Size: 617 1263 1752 2219
Fragments arranged by size:
2219 1752 1263 617
BsaAI yAC'GTr
Cuts at: 493 1204 3642 493
Size: 711 2438 2702
Fragments arranged by size:
2702 2438 711
BsaBI GATnn'nnATC
Cuts at: 5845 5845
Size: 5851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2634 3658 4040 4675
Size: 53 83 186 936 1101 1024 382 635
Cuts at: 4675 275
Size: 1451
Fragments arranged by size:
1451 1101 1024 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4284 4682 4956 5103 5514 4284
Size: 398 274 147 411 4621
Fragments arranged by size:
4621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 1178
Size: 30 165 30 1134 30 4462
Fragments arranged by size:
4462 1134 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2018 2172 3022 1186
Size: 832 154 850 4015
Fragments arranged by size:
4015 850 832 154
BseYI C'CCAG_C
Cuts at: 2135 5054 5550 2135
Size: 2919 496 2436
Fragments arranged by size:
2919 2436 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5278
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3301 4062 5090 1540
Size: 275 186 1300 761 1028 2301
Fragments arranged by size:
2301 1300 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2355 2892 3912 3997 5068 2355
Size: 537 1020 85 1071 3138
Fragments arranged by size:
3138 1071 1020 537 85
BsmI GAATG_Cn'
Cuts at: 2237 2380 3362 3455 2237
Size: 143 982 93 4633
Fragments arranged by size:
4633 982 143 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2355 2892 2925 3170 3912 3997
Size: 399 310 255 537 33 245 742 85
Cuts at: 3997 5068 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 537 399 310 255 245
85 33
BspEI T'CCGG_A
Cuts at: 5514 5514
Size: 5851
BspHI T'CATG_A
Cuts at: 3114 3740 5470 3114
Size: 626 1730 3495
Fragments arranged by size:
3495 1730 626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2184 2292 2601 2889 3066 3075 3206 844
Size: 1340 108 309 288 177 9 131 3489
Fragments arranged by size:
3489 1340 309 288 177 131 108 9
BsrBI CCG'CTC
Cuts at: 1394 1823 3738 1394
Size: 429 1915 3507
Fragments arranged by size:
3507 1915 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4346 4520 65
Size: 1183 3098 174 1396
Fragments arranged by size:
3098 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4495 4682 1700
Size: 110 2685 187 2869
Fragments arranged by size:
2869 2685 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5851
BssSI C'ACGA_G
Cuts at: 3911 4923 3911
Size: 1012 4839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5851
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 2010 1184
Size: 826 5025
BstXI CCAn_nnnn'nTGG
Cuts at: 3106 3106
Size: 5851
BstYI r'GATC_y
Cuts at: 1972 2605 3181 3548 3933 3950 5402 5488 5500
Size: 633 576 367 385 17 1452 86 12
Cuts at: 5500 5846 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 633 576 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2656 3141 513
Size: 552 1591 485 3223
Fragments arranged by size:
3223 1591 552 485
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2404 487
Size: 683 56 1178 3934
Fragments arranged by size:
3934 1178 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 3367 4161 4181 2923
Size: 444 794 20 4593
Fragments arranged by size:
4593 794 444 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5816
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4858 809
Size: 507 3542 1802
Fragments arranged by size:
3542 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3298 4187 5816
Size: 54 1425 50 275 18 1468 889 1629
Cuts at: 5816 8
Size: 43
Fragments arranged by size:
1629 1468 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3298 1537
Size: 275 1486 4090
Fragments arranged by size:
4090 1486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2487 3781 1353
Size: 1134 1294 3423
Fragments arranged by size:
3423 1294 1134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3116 4438 4812 4958 387
Size: 1064 138 1527 1322 374 146 1280
Fragments arranged by size:
1527 1322 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2353 2353
Size: 5851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2178 2315 2462 2539 2543 2560 2594
Size: 393 546 137 147 77 4 17 34
Cuts at: 2594 2606 3062 3914 4511 5298 5566 1239
Size: 12 456 852 597 787 268 1524
Fragments arranged by size:
1524 852 787 597 546 456 393 268
147 137 77 34 17 12 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2288 2288
Size: 5851
EcoO109I rG'GnC_Cy
Cuts at: 2198 2293 2497 2722 2198
Size: 95 204 225 5327
Fragments arranged by size:
5327 225 204 95
EcoRI G'AATT_C
Cuts at: 3160 3538 3160
Size: 378 5473
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 5851
FspI TGC'GCA
Cuts at: 4357 4357
Size: 5851
HaeII r_GCGC'y
Cuts at: 1678 4998 1678
Size: 3320 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 3017 3049 4492
Size: 32 135 32 668 32 745 32 1443
Cuts at: 4492 4524 4566 4598 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1443 745 668 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 3441 669
Size: 988 281 1257 246 3079
Fragments arranged by size:
3079 1257 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KpnI G_GTAC'C
Cuts at: 3176 3176
Size: 5851
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2469 2901 4965 5149 1305
Size: 1164 432 2064 184 2007
Fragments arranged by size:
2064 2007 1164 432 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4029
Fragments arranged by size:
4029 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3809 4168 4327 518
Size: 648 2643 359 159 2042
Fragments arranged by size:
2643 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2530 3106 3267 3944 5092 5337 1606
Size: 924 576 161 677 1148 245 2120
Fragments arranged by size:
2120 1148 924 677 576 245 161
NcoI C'CATG_G
Cuts at: 513 1065 2656 513
Size: 552 1591 3708
Fragments arranged by size:
3708 1591 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 3209 4754 1132
Size: 97 1980 1545 2229
Fragments arranged by size:
2229 1980 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2718 1348
Size: 1370 4481
PciI A'CATG_T
Cuts at: 4750 4750
Size: 5851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5851
PfoI T'CCnGG_A
Cuts at: 2601 2601
Size: 5851
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2537 2569 3920 3952 5433 5465 2537
Size: 32 1351 32 1481 32 2923
Fragments arranged by size:
2923 1481 1351 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2497 2722 2293
Size: 204 225 5422
Fragments arranged by size:
5422 225 204
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PspOMI G'GGCC_C
Cuts at: 2096 2096
Size: 5851
PstI C_TGCA'G
Cuts at: 830 3138 3203 830
Size: 2308 65 3478
Fragments arranged by size:
3478 2308 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
PvuII CAG'CTG
Cuts at: 2530 2530
Size: 5851
SacI G_AGCT'C
Cuts at: 2355 2355
Size: 5851
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SanDI GG'GwC_CC
Cuts at: 2722 2722
Size: 5851
SbfI CC_TGCA'GG
Cuts at: 3203 3203
Size: 5851
ScaI AGT'ACT
Cuts at: 4099 5811 4099
Size: 1712 4139
SfcI C'TryA_G
Cuts at: 826 1045 3134 3199 4334 5015 5206 826
Size: 219 2089 65 1135 681 191 1471
Fragments arranged by size:
2089 1471 1135 681 219 191 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 2054 2054
Size: 5851
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5851
SmaI CCC'GGG
Cuts at: 1872 2089 2101 3178 1872
Size: 217 12 1077 4545
Fragments arranged by size:
4545 1077 217 12
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2663 3955 4856 5118 820
Size: 197 926 720 1292 901 262 1553
Fragments arranged by size:
1553 1292 926 901 720 262 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 3209 1132
Size: 2077 3774
SrfI GCCC'GGGC
Cuts at: 2101 2101
Size: 5851
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 2385 1517
Size: 868 4983
StyI C'CwwG_G
Cuts at: 513 1065 2059 2656 3290 513
Size: 552 994 597 634 3074
Fragments arranged by size:
3074 994 634 597 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2149 3878 3895 4048 4233 4678 325
Size: 1255 569 1729 17 153 185 445 1498
Fragments arranged by size:
1729 1498 1255 569 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4097 5809 96
Size: 274 80 33 51 1443 2120 1712 138
Fragments arranged by size:
2120 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2902 2934 5811 5843 2902
Size: 32 2877 32 2910
Fragments arranged by size:
2910 2877 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2633 1316
Size: 1317 4534
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3010 3010
Size: 5851
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 2087 2099 3176 1870
Size: 217 12 1077 4545
Fragments arranged by size:
4545 1077 217 12
XmnI GAAnn'nnTTC
Cuts at: 2339 3980 2339
Size: 1641 4210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 276
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SapI SexAI SfoI SwaI