Restriction map for
pFN21AB3845
(Circular) (Six-base) MAPSORT of: pFN21AB3845.seq Check: 8970 from: 1 to: 5755
pFN21AB3845 5755 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2486 3818 5202 5470 1632
Size: 854 1332 1384 268 1917
Fragments arranged by size:
1917 1384 1332 854 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 2177 4586 2177
Size: 2409 3346
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2561 2593 2561
Size: 32 5723
AlwNI CAG_nnn'CTG
Cuts at: 3498 5070 3498
Size: 1572 4183
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2660 2705 2723 3064 3290 3442 5551 1493
Size: 1167 45 18 341 226 152 2109 1697
Fragments arranged by size:
2109 1697 1167 341 226 152 45 18
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 3080 1870
Size: 73 1137 4545
Fragments arranged by size:
4545 1137 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2083 3076 4531 618
Size: 325 217 627 269 27 993 1455 1842
Fragments arranged by size:
1842 1455 993 627 325 269 217 27
BanII G_rGCy'C
Cuts at: 1391 2019 2067 2427 3074 1391
Size: 628 48 360 647 4072
Fragments arranged by size:
4072 647 628 360 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2171 2180 928
Size: 971 272 9 4503
Fragments arranged by size:
4503 971 272 9
BbvCI CC'TCA_GC
Cuts at: 2591 2591
Size: 5755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2657 2691 3946 3980 1903
Size: 34 720 34 1255 34 3678
Fragments arranged by size:
3678 1255 720 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2088 3816 4972 1790
Size: 298 1728 1156 2573
Fragments arranged by size:
2573 1728 1156 298
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2228 3047 3486
Size: 416 53 83 399 24 811 819 439
Cuts at: 3486 4444 442
Size: 958 1753
Fragments arranged by size:
1753 958 819 811 439 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2260 2666 2915 4415 1239
Size: 1021 406 249 1500 2579
Fragments arranged by size:
2579 1500 1021 406 249
Bpu10I CC'TnA_GC
Cuts at: 2061 2073 2591 2625 2061
Size: 12 518 34 5191
Fragments arranged by size:
5191 518 34 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2924 3880 4745 5043 2594
Size: 330 956 865 298 3306
Fragments arranged by size:
3306 956 865 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 2388 4418 882
Size: 617 519 370 2030 2219
Fragments arranged by size:
2219 2030 617 519 370
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3562 3944 4579 275
Size: 53 83 186 936 2029 382 635 1451
Fragments arranged by size:
2029 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2177 3013 4188 4586 4860 5007 5418 2177
Size: 836 1175 398 274 147 411 2514
Fragments arranged by size:
2514 1175 836 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2639 2669 1178
Size: 30 165 30 1236 30 4264
Fragments arranged by size:
4264 1236 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2306 2486 2663 1186
Size: 1120 180 177 4278
Fragments arranged by size:
4278 1120 180 177
BseYI C'CCAG_C
Cuts at: 2119 2418 4958 5454 2119
Size: 299 2540 496 2420
Fragments arranged by size:
2540 2420 496 299
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2842 3205 3966 4994 1540
Size: 275 186 841 363 761 1028 2301
Fragments arranged by size:
2301 1028 841 761 363 275 186
BsiHKAI G_wGCw'C
Cuts at: 3816 3901 4972 3816
Size: 85 1071 4599
Fragments arranged by size:
4599 1071 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2067 2088 2427 3074 3816 3901
Size: 399 229 48 21 339 647 742 85
Cuts at: 3901 4972 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 647 399 339 229 85
48 21
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 2580 3644 5374 2580
Size: 1064 1730 2961
Fragments arranged by size:
2961 1730 1064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 3110 844
Size: 1280 986 3489
Fragments arranged by size:
3489 1280 986
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2619 4250 4424 65
Size: 1183 1371 1631 174 1396
Fragments arranged by size:
1631 1396 1371 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2177 4399 4586 1700
Size: 110 367 2222 187 2869
Fragments arranged by size:
2869 2222 367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 2511 3815 4827 2511
Size: 1304 1012 3439
Fragments arranged by size:
3439 1304 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstXI CCAn_nnnn'nTGG
Cuts at: 2119 2311 2644 2822 2119
Size: 192 333 178 5052
Fragments arranged by size:
5052 333 192 178
BstYI r'GATC_y
Cuts at: 1972 2298 2569 3085 3452 3837 3854 5306 5392
Size: 326 271 516 367 385 17 1452 86
Cuts at: 5392 5404 5750 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 516 385 367 346 326 271
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2852 513
Size: 552 1787 3416
Fragments arranged by size:
3416 1787 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2467 487
Size: 683 56 1241 3775
Fragments arranged by size:
3775 1241 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 3271 4065 4085 2518
Size: 753 794 20 4188
Fragments arranged by size:
4188 794 753 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2513 4762 809
Size: 507 1197 2249 1802
Fragments arranged by size:
2249 1802 1197 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2206 2839 3202
Size: 54 1425 50 275 18 376 633 363
Cuts at: 3202 4091 5720 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 633 376 363 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2839 3202 1537
Size: 275 1027 363 4090
Fragments arranged by size:
4090 1027 363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2252 3685 1353
Size: 899 1433 3423
Fragments arranged by size:
3423 1433 899
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2260 2486 2666 2915 3818 4415 5202
Size: 393 628 226 180 249 903 597 787
Cuts at: 5202 5470 1239
Size: 268 1524
Fragments arranged by size:
1524 903 787 628 597 393 268 249
226 180
EcoNI CCTnn'n_nnAGG
Cuts at: 2468 2594 2468
Size: 126 5629
EcoO109I rG'GnC_Cy
Cuts at: 2833 2922 2935 2833
Size: 89 13 5653
Fragments arranged by size:
5653 89 13
EcoRI G'AATT_C
Cuts at: 2660 2705 3064 3442 2660
Size: 45 359 378 4973
Fragments arranged by size:
4973 378 359 45
FspI TGC'GCA
Cuts at: 2159 4261 2159
Size: 2102 3653
HaeII r_GCGC'y
Cuts at: 1678 4902 1678
Size: 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2385 2417 2639 2671 2898
Size: 32 135 32 813 32 222 32 227
Cuts at: 2898 2930 4396 4428 4470 4502 1373
Size: 32 1466 32 42 32 2626
Fragments arranged by size:
2626 1466 813 227 222 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2239 2479 3099 3345 669
Size: 988 281 301 240 620 246 3079
Fragments arranged by size:
3079 988 620 301 281 246 240
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 4869 5053 1305
Size: 831 2733 184 2007
Fragments arranged by size:
2733 2007 831 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2208 10
Size: 54 1425 343 376 3557
Fragments arranged by size:
3557 1425 376 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2867 3713 4072 4231 518
Size: 648 1701 846 359 159 2042
Fragments arranged by size:
2042 1701 846 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2081 2280 3171 3848 4996 5241 1606
Size: 475 199 891 677 1148 245 2120
Fragments arranged by size:
2120 1148 891 677 475 245 199
NcoI C'CATG_G
Cuts at: 513 1065 2852 513
Size: 552 1787 3416
Fragments arranged by size:
3416 1787 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2336 2782 3113 4658 1132
Size: 97 1107 446 331 1545 2229
Fragments arranged by size:
2229 1545 1107 446 331 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2390 1108
Size: 1282 4473
PfoI T'CCnGG_A
Cuts at: 2931 2931
Size: 5755
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2833 2922 2935 2833
Size: 89 13 5653
Fragments arranged by size:
5653 89 13
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2444 2476 2444
Size: 32 5723
PstI C_TGCA'G
Cuts at: 830 3107 830
Size: 2277 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2280 2280
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SapI GCTCTTCn'nnn_
Cuts at: 2252 2252
Size: 5755
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 3103 4238 4919 5110 826
Size: 219 2058 1135 681 191 1471
Fragments arranged by size:
2058 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2573 2939 3859 4760 5022 820
Size: 197 926 630 366 920 901 262 1553
Fragments arranged by size:
1553 926 920 901 630 366 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 2782 3113 1132
Size: 1650 331 3774
Fragments arranged by size:
3774 1650 331
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 2071 2273 3006 1517
Size: 554 202 733 4266
Fragments arranged by size:
4266 733 554 202
StyI C'CwwG_G
Cuts at: 513 1065 2852 3194 513
Size: 552 1787 342 3074
Fragments arranged by size:
3074 1787 552 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3782 3799 3952 4137 4582 325
Size: 1255 2202 17 153 185 445 1498
Fragments arranged by size:
2202 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2436 4001 5713
Size: 274 80 33 51 1443 459 1565 1712
Cuts at: 5713 96
Size: 138
Fragments arranged by size:
1712 1565 1443 459 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2943 2975 5715 5747 2943
Size: 32 2740 32 2951
Fragments arranged by size:
2951 2740 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2912 1316
Size: 1596 4159
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 2050
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI