Restriction map for
pFN21AB3818
(Circular) (Six-base) MAPSORT of: pFN21AB3818.seq Check: 9298 from: 1 to: 5953
pFN21AB3818 5953 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2391 3763 278
Size: 53 83 186 936 855 1372 2468
Fragments arranged by size:
2468 1372 936 855 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2439 3114 3296 1937
Size: 502 675 182 4594
Fragments arranged by size:
4594 675 502 182
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 4080 4453 1563
Size: 2517 373 3063
Fragments arranged by size:
3063 2517 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4016 5400 5668 1632
Size: 2384 1384 268 1917
Fragments arranged by size:
2384 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5756
AflIII A'CryG_T
Cuts at: 1203 2014 4852 5829 1203
Size: 811 2838 977 1327
Fragments arranged by size:
2838 1327 977 811
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 4682 4682
Size: 5953
AleI CACnn'nnGTG
Cuts at: 1166 3150 1166
Size: 1984 3969
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3168 1184
Size: 1984 3969
AlwNI CAG_nnn'CTG
Cuts at: 3696 5268 3696
Size: 1572 4381
ApaLI G'TGCA_C
Cuts at: 4010 5166 4010
Size: 1156 4797
ApoI r'AATT_y
Cuts at: 1493 2300 2744 2885 3262 3488 3640 5749 1493
Size: 807 444 141 377 226 152 2109 1697
Fragments arranged by size:
2109 1697 807 444 377 226 152 141
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 2270 2906 2979 3245 3278 1870
Size: 73 327 636 73 266 33 4545
Fragments arranged by size:
4545 636 327 266 73 73 33
BamHI G'GATC_C
Cuts at: 2196 3207 3283 3650 2196
Size: 1011 76 367 4499
Fragments arranged by size:
4499 1011 367 76
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 3274 4729 618
Size: 325 217 627 294 1193 1455 1842
Fragments arranged by size:
1842 1455 1193 627 325 294 217
BanII G_rGCy'C
Cuts at: 1391 2633 2652 2986 3045 3272 1391
Size: 1242 19 334 59 227 4072
Fragments arranged by size:
4072 1242 334 227 59 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2138 928
Size: 971 239 4743
Fragments arranged by size:
4743 971 239
BbvCI CC'TCA_GC
Cuts at: 2223 2223
Size: 5953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2561 2595 4144 4178 1903
Size: 34 624 34 1549 34 3678
Fragments arranged by size:
3678 1549 624 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 2340 1548
Size: 792 5161
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4564 136
Size: 107 122 71 631 605 2892 1525
Fragments arranged by size:
2892 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5948 5948
Size: 5953
BlpI GC'TnA_GC
Cuts at: 3370 3370
Size: 5953
Bme1580I G_kGCm'C
Cuts at: 1790 4014 5170 1790
Size: 2224 1156 2573
Fragments arranged by size:
2573 2224 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5953
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3195 3684 4642
Size: 416 53 83 399 24 1778 489 958
Cuts at: 4642 442
Size: 1753
Fragments arranged by size:
1778 1753 958 489 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2612 4613 1239
Size: 902 471 2001 2579
Fragments arranged by size:
2579 2001 902 471
Bpu10I CC'TnA_GC
Cuts at: 2223 2223
Size: 5953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2294 4078 4943 5241 2294
Size: 1784 865 298 3006
Fragments arranged by size:
3006 1784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3205 4616 882
Size: 617 1706 1411 2219
Fragments arranged by size:
2219 1706 1411 617
BsaAI yAC'GTr
Cuts at: 493 1204 2015 2608 3744 493
Size: 711 811 593 1136 2702
Fragments arranged by size:
2702 1136 811 711 593
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2388 2478 3760 4142
Size: 53 83 186 936 855 90 1282 382
Cuts at: 4142 4777 275
Size: 635 1451
Fragments arranged by size:
1451 1282 936 855 635 382 186 90
83 53
BsaWI w'CCGG_w
Cuts at: 4386 4784 5058 5205 5616 4386
Size: 398 274 147 411 4723
Fragments arranged by size:
4723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2615 2645 3155 3185 1178
Size: 30 165 30 1212 30 510 30 3946
Fragments arranged by size:
3946 1212 510 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2960 3160 3163 1186
Size: 1774 200 3 3976
Fragments arranged by size:
3976 1774 200 3
BseYI C'CCAG_C
Cuts at: 2145 2257 2416 3211 5156 5652 2145
Size: 112 159 795 1945 496 2446
Fragments arranged by size:
2446 1945 795 496 159 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 2913 3128 1163
Size: 573 399 778 215 3988
Fragments arranged by size:
3988 778 573 399 215
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3403 4164 5192 1540
Size: 275 186 1117 285 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 285 275 186
BsiHKAI G_wGCw'C
Cuts at: 2613 4014 4099 5170 2613
Size: 1401 85 1071 3396
Fragments arranged by size:
3396 1401 1071 85
BsmI GAATG_Cn'
Cuts at: 3464 3557 3464
Size: 93 5860
BsmBI CGTCTCn'nnnn_
Cuts at: 3172 3172
Size: 5953
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2613 2633 2652 2986 3045 3272 4014
Size: 399 823 20 19 334 59 227 742
Cuts at: 4014 4099 5170 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 823 742 399 334 227 85
59 20 19
BspEI T'CCGG_A
Cuts at: 5616 5616
Size: 5953
BspHI T'CATG_A
Cuts at: 3842 5572 3842
Size: 1730 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3308 844
Size: 2464 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2055 2772 2926 3840 1394
Size: 429 232 717 154 914 3507
Fragments arranged by size:
3507 914 717 429 232 154
BsrDI GCAATG_nn'
Cuts at: 65 1248 4448 4622 65
Size: 1183 3200 174 1396
Fragments arranged by size:
3200 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2525 2838 3021 3076 4597 4784 1700
Size: 110 715 313 183 55 1521 187 2869
Fragments arranged by size:
2869 1521 715 313 187 183 110 55
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 4013 5025 4013
Size: 1012 4941
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5953
BstBI TT'CG_AA
Cuts at: 4757 4757
Size: 5953
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5953
BstXI CCAn_nnnn'nTGG
Cuts at: 2119 3080 2119
Size: 961 4992
BstYI r'GATC_y
Cuts at: 1972 2196 3207 3283 3650 4035 4052 5504 5590
Size: 224 1011 76 367 385 17 1452 86
Cuts at: 5590 5602 5948 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 224 86
76 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3003 3045 513
Size: 552 939 999 42 3421
Fragments arranged by size:
3421 999 939 552 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2074 487
Size: 683 56 848 4366
Fragments arranged by size:
4366 848 683 56
BtsI GCAGTG_nn'
Cuts at: 3012 3037 3469 4263 4283 3012
Size: 25 432 794 20 4682
Fragments arranged by size:
4682 794 432 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5918
DraI TTT'AAA
Cuts at: 3257 3257
Size: 5953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 2460 3400
Size: 54 1425 50 275 18 498 132 940
Cuts at: 3400 4289 5918 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 940 889 498 275 132 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2825 2837 3883 1353
Size: 1472 12 1046 3423
Fragments arranged by size:
3423 1472 1046 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4540 4914 5060 387
Size: 1064 138 2951 374 146 1280
Fragments arranged by size:
2951 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2612 4016 4613 5400 5668 1239
Size: 393 509 471 1404 597 787 268 1524
Fragments arranged by size:
1524 1404 787 597 509 471 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2058 2261 2275 2719 2058
Size: 203 14 444 5292
Fragments arranged by size:
5292 444 203 14
EcoRI G'AATT_C
Cuts at: 2744 3262 3640 2744
Size: 518 378 5057
Fragments arranged by size:
5057 518 378
EcoRV GAT'ATC
Cuts at: 2071 2071
Size: 5953
FspI TGC'GCA
Cuts at: 4459 4459
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 2671 5100 1678
Size: 993 2429 2531
Fragments arranged by size:
2531 2429 993
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2031 2063 4594 4626 4668
Size: 32 135 32 459 32 2531 32 42
Cuts at: 4668 4700 1373
Size: 32 2626
Fragments arranged by size:
2626 2531 459 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2706 3115 3297 3543 669
Size: 988 281 768 409 182 246 3079
Fragments arranged by size:
3079 988 768 409 281 246 182
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5953
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 3552 3552
Size: 5953
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5067 5251 1305
Size: 3762 184 2007
Fragments arranged by size:
3762 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2462 10
Size: 54 1425 343 630 3501
Fragments arranged by size:
3501 1425 630 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3150 3911 4270 4429 518
Size: 648 1984 761 359 159 2042
Fragments arranged by size:
2042 1984 761 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2149 3369 4046 5194 5439 1606
Size: 543 1220 677 1148 245 2120
Fragments arranged by size:
2120 1220 1148 677 543 245
NaeI GCC'GGC
Cuts at: 3078 3078
Size: 5953
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4462
Fragments arranged by size:
4462 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5953
NgoMIV G'CCGG_C
Cuts at: 3076 3076
Size: 5953
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5953
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NspI r_CATG'y
Cuts at: 1132 1229 2881 3311 4856 1132
Size: 97 1652 430 1545 2229
Fragments arranged by size:
2229 1652 1545 430 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5953
PciI A'CATG_T
Cuts at: 4852 4852
Size: 5953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2211 1108
Size: 1103 4850
PfoI T'CCnGG_A
Cuts at: 3203 3203
Size: 5953
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2150 2182 2264 2296 4022 4054 5535 5567 2150
Size: 32 82 32 1726 32 1481 32 2536
Fragments arranged by size:
2536 1726 1481 82 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2058 2275 2719 2058
Size: 217 444 5292
Fragments arranged by size:
5292 444 217
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 3523 3523
Size: 5953
PspXI vC'TCGA_Gb
Cuts at: 2270 2270
Size: 5953
PstI C_TGCA'G
Cuts at: 830 3305 830
Size: 2475 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
PvuII CAG'CTG
Cuts at: 2149 2149
Size: 5953
SalI G'TCGA_C
Cuts at: 1936 3113 3295 1936
Size: 1177 182 4594
Fragments arranged by size:
4594 1177 182
SapI GCTCTTCn'nnn_
Cuts at: 2825 2825
Size: 5953
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 4201 5913 4201
Size: 1712 4241
SexAI A'CCwGG_T
Cuts at: 2433 2433
Size: 5953
SfcI C'TryA_G
Cuts at: 826 1045 3301 4436 5117 5308 826
Size: 219 2256 1135 681 191 1471
Fragments arranged by size:
2256 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5953
SmaI CCC'GGG
Cuts at: 1872 3280 1872
Size: 1408 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2270 2309 4057 4958 5220 820
Size: 197 926 327 39 1748 901 262 1553
Fragments arranged by size:
1748 1553 926 901 327 262 197 39
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5953
StyI C'CwwG_G
Cuts at: 513 1065 2004 3392 513
Size: 552 939 1388 3074
Fragments arranged by size:
3074 1388 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2069 2501 3016 3980 3997 4150 4335
Size: 1255 489 432 515 964 17 153 185
Cuts at: 4335 4780 325
Size: 445 1498
Fragments arranged by size:
1498 1255 964 515 489 445 432 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 4199 5911
Size: 274 80 33 51 1443 639 1583 1712
Cuts at: 5911 96
Size: 138
Fragments arranged by size:
1712 1583 1443 639 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5913 5945 5913
Size: 32 5921
Tth111I GACn'n_nGTC
Cuts at: 1316 2861 1316
Size: 1545 4408
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XhoI C'TCGA_G
Cuts at: 1943 2270 1943
Size: 327 5626
XmaI C'CCGG_G
Cuts at: 1870 3278 1870
Size: 1408 4545
XmnI GAAnn'nnTTC
Cuts at: 4082 4082
Size: 5953
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2389 3761 276
Size: 53 83 186 936 855 1372 2468
Fragments arranged by size:
2468 1372 936 855 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI XcmI