Restriction map for
pFN21AB3816
(Circular) (Six-base) MAPSORT of: pFN21AB3816.seq Check: 9397 from: 1 to: 10741
pFN21AB3816 10741 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3190 4016 3190
Size: 826 9915
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4332 8551 278
Size: 53 83 186 936 2796 4219 2468
Fragments arranged by size:
4219 2796 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3447 5124 7341 8084 1937
Size: 1510 1677 2217 743 4594
Fragments arranged by size:
4594 2217 1677 1510 743
Acc65I G'GTAC_C
Cuts at: 2178 3045 8062 2178
Size: 867 5017 4857
Fragments arranged by size:
5017 4857 867
AclI AA'CG_TT
Cuts at: 1563 6304 8868 9241 1563
Size: 4741 2564 373 3063
Fragments arranged by size:
4741 3063 2564 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2260 2310 2801 5784 5860 6924 7835 8804
Size: 628 50 491 2983 76 1064 911 969
Cuts at: 8804 10188 10456 1632
Size: 1384 268 1917
Fragments arranged by size:
2983 1917 1384 1064 969 911 628 491
268 76 50
AfeI AGC'GCT
Cuts at: 2748 2748
Size: 10741
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10544
AflIII A'CryG_T
Cuts at: 1203 3723 3732 6475 7527 9640 10617 1203
Size: 2520 9 2743 1052 2113 977 1327
Fragments arranged by size:
2743 2520 2113 1327 1052 977 9
AgeI A'CCGG_T
Cuts at: 9572 9572
Size: 10741
AhdI GACnn_n'nnGTC
Cuts at: 3178 5985 9470 3178
Size: 2807 3485 4449
Fragments arranged by size:
4449 3485 2807
AleI CACnn'nnGTG
Cuts at: 1166 3579 6476 1166
Size: 2413 2897 5431
Fragments arranged by size:
5431 2897 2413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3172 5513 1184
Size: 1988 2341 6412
Fragments arranged by size:
6412 2341 1988
AlwNI CAG_nnn'CTG
Cuts at: 2944 3023 4955 5500 5781 6451 6970 8484 10056
Size: 79 1932 545 281 670 519 1514 1572
Cuts at: 10056 2944
Size: 3629
Fragments arranged by size:
3629 1932 1572 1514 670 545 519 281
79
ApaI G_GGCC'C
Cuts at: 6380 7155 7224 6380
Size: 775 69 9897
Fragments arranged by size:
9897 775 69
ApaLI G'TGCA_C
Cuts at: 8798 9954 8798
Size: 1156 9585
ApoI r'AATT_y
Cuts at: 1493 2250 4175 4359 5361 5745 8050 8276 8428
Size: 757 1925 184 1002 384 2305 226 152
Cuts at: 8428 10537 1493
Size: 2109 1697
Fragments arranged by size:
2305 2109 1925 1697 1002 757 384 226
184 152
AseI AT'TA_AT
Cuts at: 160 1032 4608 9295 160
Size: 872 3576 4687 1606
Fragments arranged by size:
4687 3576 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10741
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 2750 5255 7989 8066 1870
Size: 73 270 537 2505 2734 77 4545
Fragments arranged by size:
4545 2734 2505 537 270 77 73
BamHI G'GATC_C
Cuts at: 2217 8071 8438 2217
Size: 5854 367 4520
Fragments arranged by size:
5854 4520 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2134 2178 2754 3045 7754
Size: 325 217 627 347 44 576 291 4709
Cuts at: 7754 8062 9517 618
Size: 308 1455 1842
Fragments arranged by size:
4709 1842 1455 627 576 347 325 308
291 217 44
BanII G_rGCy'C
Cuts at: 1391 2379 3962 4659 4917 4929 6380 6429 6783
Size: 988 1583 697 258 12 1451 49 354
Cuts at: 6783 7000 7155 7224 7839 8060 1391
Size: 217 155 69 615 221 4072
Fragments arranged by size:
4072 1583 1451 988 697 615 354 258
221 217 155 69 49 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2064 2422 2828 3835 4287 6066 6171
Size: 971 165 358 406 1007 452 1779 105
Cuts at: 6171 928
Size: 5498
Fragments arranged by size:
5498 1779 1007 971 452 406 358 165
105
BbvCI CC'TCA_GC
Cuts at: 4143 4824 5605 7920 4143
Size: 681 781 2315 6964
Fragments arranged by size:
6964 2315 781 681
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2731 2765 8932 8966 1903
Size: 34 794 34 6167 34 3678
Fragments arranged by size:
6167 3678 794 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2004 6230 6818 8633 9843 2004
Size: 4226 588 1815 1210 2902
Fragments arranged by size:
4226 2902 1815 1210 588
BclI T'GATC_A
Cuts at: 1548 3378 4260 5703 1548
Size: 1830 882 1443 6586
Fragments arranged by size:
6586 1830 1443 882
BfrBI ATG'CAT
Cuts at: 2090 2090
Size: 10741
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9352 136
Size: 107 122 71 631 605 7680 1525
Fragments arranged by size:
7680 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2199 5541 10736 2199
Size: 3342 5195 2204
Fragments arranged by size:
5195 3342 2204
BlpI GC'TnA_GC
Cuts at: 6999 7089 8158 6999
Size: 90 1069 9582
Fragments arranged by size:
9582 1069 90
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2757 5268 6380 7155 7224 8802 9958
Size: 349 618 2511 1112 775 69 1578 1156
Cuts at: 9958 1790
Size: 2573
Fragments arranged by size:
2573 2511 1578 1156 1112 775 618 349
69
BmgBI CAC'GTC
Cuts at: 1318 2227 3384 6177 7530 1318
Size: 909 1157 2793 1353 4529
Fragments arranged by size:
4529 2793 1353 1157 909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4195 5100 5262
Size: 416 53 83 399 24 2778 905 162
Cuts at: 5262 5398 5979 6106 6424 7158 8472 9430 442
Size: 136 581 127 318 734 1314 958 1753
Fragments arranged by size:
2778 1753 1314 958 905 734 581 416
399 318 162 136 127 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10741
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6603 6603
Size: 10741
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2053 3403 5003 5351 5474 6228 7327 7567
Size: 814 1350 1600 348 123 754 1099 240
Cuts at: 7567 7853 9401 1239
Size: 286 1548 2579
Fragments arranged by size:
2579 1600 1548 1350 1099 814 754 348
286 240 123
Bpu10I CC'TnA_GC
Cuts at: 2760 2910 4143 4712 4824 5605 7769 7774 7920
Size: 150 1233 569 112 781 2164 5 146
Cuts at: 7920 2760
Size: 5581
Fragments arranged by size:
5581 2164 1233 781 569 150 146 112
5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3171 3332 3985 5627 6767 8866 9731 10029 3171
Size: 161 653 1642 1140 2099 865 298 3883
Fragments arranged by size:
3883 2099 1642 1140 865 653 298 161
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3868 7025 7414 9404 882
Size: 617 2369 3157 389 1990 2219
Fragments arranged by size:
3157 2369 2219 1990 617 389
BsaAI yAC'GTr
Cuts at: 493 1204 2932 3321 3726 3775 8532 493
Size: 711 1728 389 405 49 4757 2702
Fragments arranged by size:
4757 2702 1728 711 405 389 49
BsaBI GATnn'nnATC
Cuts at: 10735 10735
Size: 10741
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4329 8548 8930 9565
Size: 53 83 186 936 2796 4219 382 635
Cuts at: 9565 275
Size: 1451
Fragments arranged by size:
4219 2796 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2615 4896 9174 9572 9846 9993 10404 2615
Size: 2281 4278 398 274 147 411 2952
Fragments arranged by size:
4278 2952 2281 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 3710 3740 4524
Size: 30 165 30 648 30 1629 30 784
Cuts at: 4524 4554 4847 4877 5511 5541 6709 6739 7609
Size: 30 293 30 634 30 1168 30 870
Cuts at: 7609 7638 7639 7668 1178
Size: 29 1 29 4251
Fragments arranged by size:
4251 1629 1168 870 784 648 634 293
165 30 30 30 30 30 30 30
30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2440 6025 6871 7417 7849 7858 1186
Size: 1024 230 3585 846 546 432 9 4069
Fragments arranged by size:
4069 3585 1024 846 546 432 230 9
BseYI C'CCAG_C
Cuts at: 2662 2717 4190 4464 4916 5413 6296 7217 7939
Size: 55 1473 274 452 497 883 921 722
Cuts at: 7939 9944 10440 2662
Size: 2005 496 2963
Fragments arranged by size:
2963 2005 1473 921 883 722 497 496
452 274 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3218 3439 4414 5216 5844 1163
Size: 573 1482 221 975 802 628 6060
Fragments arranged by size:
6060 1482 975 802 628 573 221
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7181 8191 8952 9980 1540
Size: 275 186 5180 1010 761 1028 2301
Fragments arranged by size:
5180 2301 1028 1010 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3962 4044 4929 6840 8802 8887 9958 3962
Size: 82 885 1911 1962 85 1071 4745
Fragments arranged by size:
4745 1962 1911 1071 885 85 82
BsmI GAATG_Cn'
Cuts at: 3522 4772 6981 7213 8252 8345 3522
Size: 1250 2209 232 1039 93 5918
Fragments arranged by size:
5918 2209 1250 1039 232 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2482 2615 5514 2482
Size: 133 2899 7709
Fragments arranged by size:
7709 2899 133
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2379 2757 3962 4044 4659 4917
Size: 399 349 240 378 1205 82 615 258
Cuts at: 4917 4929 5268 6380 6429 6783 6840 7000 7155
Size: 12 339 1112 49 354 57 160 155
Cuts at: 7155 7224 7839 8060 8802 8887 9958 1391
Size: 69 615 221 742 85 1071 2174
Fragments arranged by size:
2174 1205 1112 1071 742 615 615 399
378 354 349 339 258 240 221 160
155 85 82 69 57 49 12
BspEI T'CCGG_A
Cuts at: 10404 10404
Size: 10741
BspHI T'CATG_A
Cuts at: 6918 8630 10360 6918
Size: 1712 1730 7299
Fragments arranged by size:
7299 1730 1712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3190 3639 4016 6624 7602 8096 844
Size: 2346 449 377 2608 978 494 3489
Fragments arranged by size:
3489 2608 2346 978 494 449 377
BsrBI CCG'CTC
Cuts at: 1394 1823 3185 8628 1394
Size: 429 1362 5443 3507
Fragments arranged by size:
5443 3507 1362 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2425 3058 4252 6081 9236 9410 65
Size: 1183 1177 633 1194 1829 3155 174 1396
Fragments arranged by size:
3155 1829 1396 1194 1183 1177 633 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3271 3906 5213 9385 9572 1700
Size: 110 1461 635 1307 4172 187 2869
Fragments arranged by size:
4172 2869 1461 1307 635 187 110
BsrGI T'GTAC_A
Cuts at: 96 2560 96
Size: 2464 8277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10741
BssSI C'ACGA_G
Cuts at: 3955 7873 8801 9813 3955
Size: 3918 928 1012 4883
Fragments arranged by size:
4883 3918 1012 928
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4561 6970 794
Size: 3767 2409 4565
Fragments arranged by size:
4565 3767 2409
BstBI TT'CG_AA
Cuts at: 9545 9545
Size: 10741
BstEII G'GTnAC_C
Cuts at: 1184 3931 4148 5072 6793 7691 1184
Size: 2747 217 924 1721 898 4234
Fragments arranged by size:
4234 2747 1721 924 898 217
BstXI CCAn_nnnn'nTGG
Cuts at: 4081 4528 5401 7831 7846 4081
Size: 447 873 2430 15 6976
Fragments arranged by size:
6976 2430 873 447 15
BstYI r'GATC_y
Cuts at: 1972 2199 2217 2513 5541 6101 8071 8438 8823
Size: 227 18 296 3028 560 1970 367 385
Cuts at: 8823 8840 10292 10378 10390 10736 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
3028 1977 1970 1452 560 385 367 346
296 227 86 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 4968 6415 7638 4968
Size: 1447 1223 8071
Fragments arranged by size:
8071 1447 1223
BtgI C'CryG_G
Cuts at: 513 1065 2121 2187 2535 2712 4091 4627 4722
Size: 552 1056 66 348 177 1379 536 95
Cuts at: 4722 5056 5580 5613 5897 7050 7824 7905 513
Size: 334 524 33 284 1153 774 81 3349
Fragments arranged by size:
3349 1379 1153 1056 774 552 536 524
348 334 284 177 95 81 66 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2687 4382 487
Size: 683 56 1461 1695 6846
Fragments arranged by size:
6846 1695 1461 683 56
BtsI GCAGTG_nn'
Cuts at: 3456 5827 6278 7617 7976 8257 9051 9071 3456
Size: 2371 451 1339 359 281 794 20 5126
Fragments arranged by size:
5126 2371 1339 794 451 359 281 20
ClaI AT'CG_AT
Cuts at: 1554 2608 1554
Size: 1054 9687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4828 4863 529
Size: 35 4264 35 6407
Fragments arranged by size:
6407 4264 35 35
DraI TTT'AAA
Cuts at: 6120 8045 6120
Size: 1925 8816
DraIII CAC_nnn'GTG
Cuts at: 7512 7512
Size: 10741
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3443 9748 809
Size: 507 2127 6305 1802
Fragments arranged by size:
6305 2127 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3904 3908 4225
Size: 54 1425 50 275 18 2074 4 317
Cuts at: 4225 4398 4836 7178 7446 7685 7908 8188 9077
Size: 173 438 2342 268 239 223 280 889
Cuts at: 9077 10706 8
Size: 1629 43
Fragments arranged by size:
2342 2074 1629 1425 889 438 317 280
275 268 239 223 173 54 50 43
18 4
EagI C'GGCC_G
Cuts at: 1537 1812 7178 8188 1537
Size: 275 5366 1010 4090
Fragments arranged by size:
5366 4090 1010 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4530 5803 7008 7470 7645 8671 1353
Size: 3177 1273 1205 462 175 1026 3423
Fragments arranged by size:
3423 3177 1273 1205 1026 462 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2210 3780 4955 6022 7852 9328
Size: 1064 138 621 1570 1175 1067 1830 1476
Cuts at: 9328 9702 9848 387
Size: 374 146 1280
Fragments arranged by size:
1830 1570 1476 1280 1175 1067 1064 621
374 146 138
EcoICRI GAG'CTC
Cuts at: 3960 4927 3960
Size: 967 9774
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2260 2310 2801 3403 5003 5351
Size: 393 421 207 50 491 602 1600 348
Cuts at: 5351 5474 5784 5860 6228 6924 7327 7567 7835
Size: 123 310 76 368 696 403 240 268
Cuts at: 7835 7853 8804 9401 10188 10456 1239
Size: 18 951 597 787 268 1524
Fragments arranged by size:
1600 1524 951 787 696 602 597 491
421 403 393 368 348 310 268 268
240 207 123 76 50 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3488 4301 3488
Size: 813 9928
EcoO109I rG'GnC_Cy
Cuts at: 3512 5222 5573 5938 6546 6582 7152 7452 7765
Size: 1710 351 365 608 36 570 300 313
Cuts at: 7765 3512
Size: 6488
Fragments arranged by size:
6488 1710 608 570 365 351 313 300
36
EcoRI G'AATT_C
Cuts at: 5361 5745 8050 8428 5361
Size: 384 2305 378 7674
Fragments arranged by size:
7674 2305 384 378
EcoRV GAT'ATC
Cuts at: 4852 4852
Size: 10741
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5368 5368
Size: 10741
FseI GG_CCGG'CC
Cuts at: 3910 3910
Size: 10741
FspI TGC'GCA
Cuts at: 3167 9247 3167
Size: 6080 4661
HaeII r_GCGC'y
Cuts at: 1678 2643 2750 7822 9888 1678
Size: 965 107 5072 2066 2531
Fragments arranged by size:
5072 2531 2066 965 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2051 2083 2511
Size: 32 135 32 430 32 17 32 428
Cuts at: 2511 2543 3164 3196 3577 3609 3961 3993 4534
Size: 32 621 32 381 32 352 32 541
Cuts at: 4534 4566 4976 5008 5511 5543 5749 5781 5971
Size: 32 410 32 503 32 206 32 190
Cuts at: 5971 6003 6559 6591 7031 7063 7096 7128 7517
Size: 32 556 32 440 32 33 32 389
Cuts at: 7517 7549 9382 9414 9456 9488 1373
Size: 32 1833 32 42 32 2626
Fragments arranged by size:
2626 1833 621 556 541 503 440 430
428 410 389 381 352 206 190 135
42 33 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 5125 7062 7545 8085 8331 669
Size: 988 281 3187 1937 483 540 246 3079
Fragments arranged by size:
3187 3079 1937 988 540 483 281 246
HindIII A'AGCT_T
Cuts at: 748 2272 6031 748
Size: 1524 3759 5458
Fragments arranged by size:
5458 3759 1524
HpaI GTT'AAC
Cuts at: 8331 8331
Size: 10741
KpnI G_GTAC'C
Cuts at: 2182 3049 8066 2182
Size: 867 5017 4857
Fragments arranged by size:
5017 4857 867
MfeI C'AATT_G
Cuts at: 4132 4212 8340 4132
Size: 80 4128 6533
Fragments arranged by size:
6533 4128 80
MluI A'CGCG_T
Cuts at: 6475 10617 6475
Size: 4142 6599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2916 3366 9855 10039 1305
Size: 1611 450 6489 184 2007
Fragments arranged by size:
6489 2007 1611 450 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4400 7448 7910 10
Size: 54 1425 343 2568 3048 462 2841
Fragments arranged by size:
3048 2841 2568 1425 462 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3154 3579 4880 5495 6476 7844 8699
Size: 648 1988 425 1301 615 981 1368 855
Cuts at: 8699 9058 9217 518
Size: 359 159 2042
Fragments arranged by size:
2042 1988 1368 1301 981 855 648 615
425 359 159
MspA1I CmG'CkG
Cuts at: 1606 2315 2592 2775 3453 3838 4034 4464 4828
Size: 709 277 183 678 385 196 430 364
Cuts at: 4828 5133 5582 5597 6078 7924 8157 8834 9982
Size: 305 449 15 481 1846 233 677 1148
Cuts at: 9982 10227 1606
Size: 245 2120
Fragments arranged by size:
2120 1846 1148 709 678 677 481 449
430 385 364 305 277 245 233 196
183 15
NaeI GCC'GGC
Cuts at: 3908 5215 3908
Size: 1307 9434
NcoI C'CATG_G
Cuts at: 513 1065 2712 4722 5613 5897 513
Size: 552 1647 2010 891 284 5357
Fragments arranged by size:
5357 2010 1647 891 552 284
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10741
NgoMIV G'CCGG_C
Cuts at: 3906 5213 3906
Size: 1307 9434
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10741
NotI GC'GGCC_GC
Cuts at: 8188 8188
Size: 10741
NruI TCG'CGA
Cuts at: 2042 2042
Size: 10741
NsiI A_TGCA'T
Cuts at: 2092 2092
Size: 10741
NspI r_CATG'y
Cuts at: 1132 1229 2794 4271 4885 7788 8099 9644 1132
Size: 97 1565 1477 614 2903 311 1545 2229
Fragments arranged by size:
2903 2229 1565 1545 1477 614 311 97
PasI CC'CwG_GG
Cuts at: 1348 3917 6380 6993 7323 1348
Size: 2569 2463 613 330 4766
Fragments arranged by size:
4766 2569 2463 613 330
PciI A'CATG_T
Cuts at: 9640 9640
Size: 10741
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3612 3917 4170 5500 6564 7251 7305 1108
Size: 2504 305 253 1330 1064 687 54 4544
Fragments arranged by size:
4544 2504 1330 1064 687 305 253 54
PfoI T'CCnGG_A
Cuts at: 3432 7830 3432
Size: 4398 6343
PmeI GTTT'AAAC
Cuts at: 8045 8045
Size: 10741
PmlI CAC'GTG
Cuts at: 3321 3726 3775 3321
Size: 405 49 10287
Fragments arranged by size:
10287 405 49
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6416 6448 8810 8842 10323 10355 6416
Size: 32 2362 32 1481 32 6802
Fragments arranged by size:
6802 2362 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5573 5938 6582 7765 5573
Size: 365 644 1183 8549
Fragments arranged by size:
8549 1183 644 365
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10741
PsiI TTA'TAA
Cuts at: 3116 8311 3116
Size: 5195 5546
PspOMI G'GGCC_C
Cuts at: 6376 7151 7220 6376
Size: 775 69 9897
Fragments arranged by size:
9897 775 69
PstI C_TGCA'G
Cuts at: 830 3011 4557 5953 6249 6621 6645 7333 7613
Size: 2181 1546 1396 296 372 24 688 280
Cuts at: 7613 8093 830
Size: 480 3478
Fragments arranged by size:
3478 2181 1546 1396 688 480 372 296
280 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10741
PvuII CAG'CTG
Cuts at: 2315 4828 6078 2315
Size: 2513 1250 6978
Fragments arranged by size:
6978 2513 1250
SacI G_AGCT'C
Cuts at: 3962 4929 3962
Size: 967 9774
SacII CC_GC'GG
Cuts at: 5583 5583
Size: 10741
SalI G'TCGA_C
Cuts at: 1936 5123 8083 1936
Size: 3187 2960 4594
Fragments arranged by size:
4594 3187 2960
SanDI GG'GwC_CC
Cuts at: 5938 5938
Size: 10741
SapI GCTCTTCn'nnn_
Cuts at: 7470 7470
Size: 10741
SbfI CC_TGCA'GG
Cuts at: 6621 8093 6621
Size: 1472 9269
ScaI AGT'ACT
Cuts at: 6251 8989 10701 6251
Size: 2738 1712 6291
Fragments arranged by size:
6291 2738 1712
SexAI A'CCwGG_T
Cuts at: 6629 6629
Size: 10741
SfcI C'TryA_G
Cuts at: 826 1045 3007 4553 4870 5485 5740 5949 6245
Size: 219 1962 1546 317 615 255 209 296
Cuts at: 6245 6617 6641 7329 7609 8089 9224 9905 10096
Size: 372 24 688 280 480 1135 681 191
Cuts at: 10096 826
Size: 1471
Fragments arranged by size:
1962 1546 1471 1135 688 681 615 480
372 317 296 280 255 219 209 191
24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10741
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10741
SmaI CCC'GGG
Cuts at: 1872 2215 7991 8068 1872
Size: 343 5776 77 4545
Fragments arranged by size:
5776 4545 343 77
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3186 3347 3964 5255 5642 6782
Size: 197 926 1243 161 617 1291 387 1140
Cuts at: 6782 8845 9746 10008 820
Size: 2063 901 262 1553
Fragments arranged by size:
2063 1553 1291 1243 1140 926 901 617
387 262 197 161
SnaBI TAC'GTA
Cuts at: 493 8532 493
Size: 8039 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10741
SphI G_CATG'C
Cuts at: 1132 7788 8099 1132
Size: 6656 311 3774
Fragments arranged by size:
6656 3774 311
SspI AAT'ATT
Cuts at: 5 52 3946 8665 5
Size: 47 3894 4719 2081
Fragments arranged by size:
4719 3894 2081 47
StuI AGG'CCT
Cuts at: 1517 5201 5720 1517
Size: 3684 519 6538
Fragments arranged by size:
6538 3684 519
StyI C'CwwG_G
Cuts at: 513 1065 2712 3925 4521 4722 5343 5613 5897
Size: 552 1647 1213 596 201 621 270 284
Cuts at: 5897 7212 8180 513
Size: 1315 968 3074
Fragments arranged by size:
3074 1647 1315 1213 968 621 596 552
284 270 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4494 5405 5773 5998 7336 8768 8785
Size: 1255 2914 911 368 225 1338 1432 17
Cuts at: 8785 8938 9123 9568 325
Size: 153 185 445 1498
Fragments arranged by size:
2914 1498 1432 1338 1255 911 445 368
225 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2560 3720 5082
Size: 274 80 33 51 1443 583 1160 1362
Cuts at: 5082 5475 5667 5772 6130 6249 6540 6716 7563
Size: 393 192 105 358 119 291 176 847
Cuts at: 7563 8987 10699 96
Size: 1424 1712 138
Fragments arranged by size:
1712 1443 1424 1362 1160 847 583 393
358 291 274 192 176 138 119 105
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2944 2976 3066 3098 3710 3742 4524 4556 4847
Size: 32 90 32 612 32 782 32 291
Cuts at: 4847 4879 5041 5073 7609 7641 10701 10733 2944
Size: 32 162 32 2536 32 3060 32 2952
Fragments arranged by size:
3060 2952 2536 782 612 291 162 90
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5525 5912 6175 1316
Size: 4209 387 263 5882
Fragments arranged by size:
5882 4209 387 263
XbaI T'CTAG_A
Cuts at: 2516 8077 2516
Size: 5561 5180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2128 2772 4525 2011
Size: 117 644 1753 8227
Fragments arranged by size:
8227 1753 644 117
XhoI C'TCGA_G
Cuts at: 1943 5255 1943
Size: 3312 7429
XmaI C'CCGG_G
Cuts at: 1870 2213 7989 8066 1870
Size: 343 5776 77 4545
Fragments arranged by size:
5776 4545 343 77
XmnI GAAnn'nnTTC
Cuts at: 2048 5171 8870 2048
Size: 3123 3699 3919
Fragments arranged by size:
3919 3699 3123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4330 8549 276
Size: 53 83 186 936 2796 4219 2468
Fragments arranged by size:
4219 2796 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BsiWI BstZ17I FspAI
KasI NarI PacI PspXI PsrI RsrII SfiI SfoI
SrfI SwaI