Restriction map for
pFN21AB3815
(Circular) (Six-base) MAPSORT of: pFN21AB3815.seq Check: 6480 from: 1 to: 5914
pFN21AB3815 5914 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3121 3121
Size: 5914
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3724 278
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2207 2945 3257 1937
Size: 270 738 312 4594
Fragments arranged by size:
4594 738 312 270
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3977 5361 5629 1632
Size: 2345 1384 268 1917
Fragments arranged by size:
2345 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 4813 5790 1203
Size: 3610 977 1327
Fragments arranged by size:
3610 1327 977
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 4643 4643
Size: 5914
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2163 1184
Size: 979 4935
AlwNI CAG_nnn'CTG
Cuts at: 2216 3657 5229 2216
Size: 1441 1572 2901
Fragments arranged by size:
2901 1572 1441
ApaLI G'TGCA_C
Cuts at: 3971 5127 3971
Size: 1156 4758
ApoI r'AATT_y
Cuts at: 1493 3200 3223 3449 3601 5710 1493
Size: 1707 23 226 152 2109 1697
Fragments arranged by size:
2109 1707 1697 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 3239 1870
Size: 73 1296 4545
Fragments arranged by size:
4545 1296 73
AvrII C'CTAG_G
Cuts at: 2566 2566
Size: 5914
BamHI G'GATC_C
Cuts at: 2710 3244 3611 2710
Size: 534 367 5013
Fragments arranged by size:
5013 534 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 2399 2467 2698 2778
Size: 325 217 627 579 33 68 231 80
Cuts at: 2778 3235 4690 618
Size: 457 1455 1842
Fragments arranged by size:
1842 1455 627 579 457 325 231 217
80 68 33
BanII G_rGCy'C
Cuts at: 1391 2046 2174 3233 1391
Size: 655 128 1059 4072
Fragments arranged by size:
4072 1059 655 128
BbeI G_GCGC'C
Cuts at: 2782 2782
Size: 5914
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2212 928
Size: 971 313 4630
Fragments arranged by size:
4630 971 313
BbvCI CC'TCA_GC
Cuts at: 2637 2637
Size: 5914
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2083 2117 4105 4139 1903
Size: 34 146 34 1988 34 3678
Fragments arranged by size:
3678 1988 146 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3042 3161 4525
Size: 107 122 71 631 605 1370 119 1364
Cuts at: 4525 136
Size: 1525
Fragments arranged by size:
1525 1370 1364 631 605 122 119 107
71
BglII A'GATC_T
Cuts at: 2499 5909 2499
Size: 3410 2504
BlpI GC'TnA_GC
Cuts at: 2045 3189 3331 2045
Size: 1144 142 4628
Fragments arranged by size:
4628 1144 142
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2404 2824 3047 3975 5131 1790
Size: 362 252 420 223 928 1156 2573
Fragments arranged by size:
2573 1156 928 420 362 252 223
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5914
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2902 3645 4603
Size: 416 53 83 399 24 1485 743 958
Cuts at: 4603 442
Size: 1753
Fragments arranged by size:
1753 1485 958 743 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2281 2289 2537 2636 4574 1239
Size: 809 233 8 248 99 1938 2579
Fragments arranged by size:
2579 1938 809 248 233 99 8
Bpu10I CC'TnA_GC
Cuts at: 2637 2637
Size: 5914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2647 3068 4039 4904 5202 2647
Size: 421 971 865 298 3359
Fragments arranged by size:
3359 971 865 421 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2896 4577 882
Size: 617 1397 1681 2219
Fragments arranged by size:
2219 1681 1397 617
BsaAI yAC'GTr
Cuts at: 493 1204 3705 493
Size: 711 2501 2702
Fragments arranged by size:
2702 2501 711
BsaBI GATnn'nnATC
Cuts at: 2159 5908 2159
Size: 3749 2165
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2779 2960 3721 4103
Size: 53 83 186 936 1246 181 761 382
Cuts at: 4103 4738 275
Size: 635 1451
Fragments arranged by size:
1451 1246 936 761 635 382 186 181
83 53
BsaWI w'CCGG_w
Cuts at: 2887 4347 4745 5019 5166 5577 2887
Size: 1460 398 274 147 411 3224
Fragments arranged by size:
3224 1460 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2869 2899 1178
Size: 30 165 30 1466 30 4193
Fragments arranged by size:
4193 1466 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2048 1186
Size: 859 3 5052
Fragments arranged by size:
5052 859 3
BseYI C'CCAG_C
Cuts at: 2632 2690 2855 2963 3040 5117 5613 2632
Size: 58 165 108 77 2077 496 2933
Fragments arranged by size:
2933 2077 496 165 108 77 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3364 4125 5153 1540
Size: 275 186 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 3975 4060 5131 2046
Size: 1929 85 1071 2829
Fragments arranged by size:
2829 1929 1071 85
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
BsmBI CGTCTCn'nnnn_
Cuts at: 2927 2927
Size: 5914
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2152 2174 2404 2824 3047 3233
Size: 399 256 106 22 230 420 223 186
Cuts at: 3233 3975 4060 5131 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 420 399 256 230 223
186 106 85 22
BspEI T'CCGG_A
Cuts at: 5577 5577
Size: 5914
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2433 2991 3121 3269 844
Size: 1589 558 130 148 3489
Fragments arranged by size:
3489 1589 558 148 130
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 2863 3193 3801 1394
Size: 429 187 853 330 608 3507
Fragments arranged by size:
3507 853 608 429 330 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 4409 4583 65
Size: 1183 3161 174 1396
Fragments arranged by size:
3161 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4558 4745 1700
Size: 110 2748 187 2869
Fragments arranged by size:
2869 2748 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5914
BssSI C'ACGA_G
Cuts at: 3974 4986 3974
Size: 1012 4902
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5914
BstBI TT'CG_AA
Cuts at: 4718 4718
Size: 5914
BstEII G'GTnAC_C
Cuts at: 1184 2641 1184
Size: 1457 4457
BstXI CCAn_nnnn'nTGG
Cuts at: 2902 2902
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 2382 2499 2710 2802 3244 3611 3996 4013
Size: 410 117 211 92 442 367 385 17
Cuts at: 4013 5465 5551 5563 5909 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 442 410 385 367 346 211
117 92 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2881 487
Size: 683 56 1655 3520
Fragments arranged by size:
3520 1655 683 56
BtsI GCAGTG_nn'
Cuts at: 2152 2919 3150 3430 4224 4244 2152
Size: 767 231 280 794 20 3822
Fragments arranged by size:
3822 794 767 280 231 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 3218 3218
Size: 5914
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2941 4921 809
Size: 507 1625 1980 1802
Fragments arranged by size:
1980 1802 1625 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2937 3361 4250
Size: 54 1425 50 275 18 1107 424 889
Cuts at: 4250 5879 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1107 889 424 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3361 1537
Size: 275 1549 4090
Fragments arranged by size:
4090 1549 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3844 1353
Size: 2491 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4501 4875 5021 387
Size: 1064 138 2912 374 146 1280
Fragments arranged by size:
2912 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2044 2044
Size: 5914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2281 2289 2537 2636 3977 4574
Size: 393 416 233 8 248 99 1341 597
Cuts at: 4574 5361 5629 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1341 787 597 416 393 268 248
233 99 8
EcoO109I rG'GnC_Cy
Cuts at: 2260 2422 2260
Size: 162 5752
EcoRI G'AATT_C
Cuts at: 3223 3601 3223
Size: 378 5536
FspI TGC'GCA
Cuts at: 4420 4420
Size: 5914
HaeII r_GCGC'y
Cuts at: 1678 2782 2856 5061 1678
Size: 1104 74 2205 2531
Fragments arranged by size:
2531 2205 1104 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2526 2558 4555 4587 4629
Size: 32 135 32 954 32 1997 32 42
Cuts at: 4629 4661 1373
Size: 32 2626
Fragments arranged by size:
2626 1997 954 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3504 669
Size: 988 281 1320 246 3079
Fragments arranged by size:
3079 1320 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5914
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KasI G'GCGC_C
Cuts at: 2778 2778
Size: 5914
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 3513 3513
Size: 5914
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3093 5028 5212 1305
Size: 1788 1935 184 2007
Fragments arranged by size:
2007 1935 1788 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4092
Fragments arranged by size:
4092 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3872 4231 4390 518
Size: 648 2706 359 159 2042
Fragments arranged by size:
2706 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2216 2636 3330 4007 5155 5400 1606
Size: 610 420 694 677 1148 245 2120
Fragments arranged by size:
2120 1148 694 677 610 420 245
NarI GG'CG_CC
Cuts at: 2779 2779
Size: 5914
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5362
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5914
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5914
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 2870 3272 4817 1132
Size: 97 1641 402 1545 2229
Fragments arranged by size:
2229 1641 1545 402 97
PasI CC'CwG_GG
Cuts at: 1348 3122 1348
Size: 1774 4140
PciI A'CATG_T
Cuts at: 4813 4813
Size: 5914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5914
PfoI T'CCnGG_A
Cuts at: 2133 2514 2133
Size: 381 5533
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2260 2422 2260
Size: 162 5752
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 3484 3484
Size: 5914
PstI C_TGCA'G
Cuts at: 830 3266 830
Size: 2436 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
PvuII CAG'CTG
Cuts at: 2216 2636 2216
Size: 420 5494
SacI G_AGCT'C
Cuts at: 2046 2046
Size: 5914
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SbfI CC_TGCA'GG
Cuts at: 3266 3266
Size: 5914
ScaI AGT'ACT
Cuts at: 4162 5874 4162
Size: 1712 4202
SfcI C'TryA_G
Cuts at: 826 1045 2208 3262 4397 5078 5269 826
Size: 219 1163 1054 1135 681 191 1471
Fragments arranged by size:
1471 1163 1135 1054 681 219 191
SfoI GGC'GCC
Cuts at: 2780 2780
Size: 5914
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 3241 1872
Size: 1369 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2662 3083 4018 4919 5181 820
Size: 197 926 719 421 935 901 262 1553
Fragments arranged by size:
1553 935 926 901 719 421 262 197
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 3272 1132
Size: 2140 3774
SspI AAT'ATT
Cuts at: 5 52 3838 5
Size: 47 3786 2081
Fragments arranged by size:
3786 2081 47
StuI AGG'CCT
Cuts at: 1517 2302 1517
Size: 785 5129
StyI C'CwwG_G
Cuts at: 513 1065 2566 3353 513
Size: 552 1501 787 3074
Fragments arranged by size:
3074 1501 787 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2542 2704 3941 3958 4111 4296 4741
Size: 1255 962 162 1237 17 153 185 445
Cuts at: 4741 325
Size: 1498
Fragments arranged by size:
1498 1255 1237 962 445 185 162 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4160 5872 96
Size: 274 80 33 51 1443 2183 1712 138
Fragments arranged by size:
2183 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2869 2901 5874 5906 2869
Size: 32 2973 32 2877
Fragments arranged by size:
2973 2877 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5914
XbaI T'CTAG_A
Cuts at: 2929 3250 2929
Size: 321 5593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2615 2692 3097 2615
Size: 77 405 5432
Fragments arranged by size:
5432 405 77
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5914
XmaI C'CCGG_G
Cuts at: 1870 3239 1870
Size: 1369 4545
XmnI GAAnn'nnTTC
Cuts at: 4043 4043
Size: 5914
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3722 276
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI