Restriction map for
pFN21AB3810
(Circular) (Six-base) MAPSORT of: pFN21AB3810.seq Check: 6721 from: 1 to: 6205
pFN21AB3810 6205 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3548 1937
Size: 1611 4594
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2046 2345 2747 3377 4268 5652 5920 1632
Size: 414 299 402 630 891 1384 268 1917
Fragments arranged by size:
1917 1384 891 630 414 402 299 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 5104 6081 1203
Size: 3901 977 1327
Fragments arranged by size:
3901 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 2492 1166
Size: 1326 4879
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2510 1184
Size: 1326 4879
AlwNI CAG_nnn'CTG
Cuts at: 3948 5520 3948
Size: 1572 4633
ApaLI G'TGCA_C
Cuts at: 2621 2658 4262 5418 2621
Size: 37 1604 1156 3408
Fragments arranged by size:
3408 1604 1156 37
ApoI r'AATT_y
Cuts at: 1493 3514 3740 3892 6001 1493
Size: 2021 226 152 2109 1697
Fragments arranged by size:
2109 2021 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 2905 3530 1870
Size: 73 962 625 4545
Fragments arranged by size:
4545 962 625 73
BamHI G'GATC_C
Cuts at: 3157 3397 3535 3902 3157
Size: 240 138 367 5460
Fragments arranged by size:
5460 367 240 138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3526 4981 618
Size: 325 217 627 1739 1455 1842
Fragments arranged by size:
1842 1739 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2802 2906 3524 1391
Size: 1411 104 618 4072
Fragments arranged by size:
4072 1411 618 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2511 2545 4396 4430 1903
Size: 34 574 34 1851 34 3678
Fragments arranged by size:
3678 1851 574 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4097 5307 4097
Size: 1210 4995
BclI T'GATC_A
Cuts at: 1548 2925 1548
Size: 1377 4828
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4816 136
Size: 107 122 71 631 605 3144 1525
Fragments arranged by size:
3144 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6200 6200
Size: 6205
BlpI GC'TnA_GC
Cuts at: 3622 3622
Size: 6205
Bme1580I G_kGCm'C
Cuts at: 1790 2625 2662 3108 4266 5422 1790
Size: 835 37 446 1158 1156 2573
Fragments arranged by size:
2573 1158 1156 835 446 37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3936 4894 442
Size: 416 53 83 399 24 2519 958 1753
Fragments arranged by size:
2519 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 4865 1239
Size: 1283 2343 2579
Fragments arranged by size:
2579 2343 1283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3111 4330 5195 5493 3111
Size: 1219 865 298 3823
Fragments arranged by size:
3823 1219 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3186 3429 4868 882
Size: 617 1687 243 1439 2219
Fragments arranged by size:
2219 1687 1439 617 243
BsaAI yAC'GTr
Cuts at: 493 1204 3996 493
Size: 711 2792 2702
Fragments arranged by size:
2792 2702 711
BsaBI GATnn'nnATC
Cuts at: 6199 6199
Size: 6205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4012 4394 5029 275
Size: 53 83 186 936 2479 382 635 1451
Fragments arranged by size:
2479 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2310 2604 2806 4638 5036 5310 5457 5868 2310
Size: 294 202 1832 398 274 147 411 2647
Fragments arranged by size:
2647 1832 411 398 294 274 202 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2248 2278 1178
Size: 30 165 30 845 30 5105
Fragments arranged by size:
5105 845 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6205
BseYI C'CCAG_C
Cuts at: 3366 5408 5904 3366
Size: 2042 496 3667
Fragments arranged by size:
3667 2042 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2194 3207 3413 1163
Size: 573 458 1013 206 3955
Fragments arranged by size:
3955 1013 573 458 206
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3655 4416 5444 1540
Size: 275 186 1654 761 1028 2301
Fragments arranged by size:
2301 1654 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2508 2625 2662 2802 2906 4266 4351 5422 2508
Size: 117 37 140 104 1360 85 1071 3291
Fragments arranged by size:
3291 1360 1071 140 117 104 85 37
BsmI GAATG_Cn'
Cuts at: 3716 3809 3716
Size: 93 6112
BsmBI CGTCTCn'nnnn_
Cuts at: 2354 2354
Size: 6205
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2508 2625 2662 2802 2906 3108 3524
Size: 399 718 117 37 140 104 202 416
Cuts at: 3524 4266 4351 5422 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 718 416 399 202 140
117 104 85 37
BspEI T'CCGG_A
Cuts at: 2604 5868 2604
Size: 3264 2941
BspHI T'CATG_A
Cuts at: 4094 5824 4094
Size: 1730 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2777 3560 844
Size: 1933 783 3489
Fragments arranged by size:
3489 1933 783
BsrBI CCG'CTC
Cuts at: 1394 1823 4092 1394
Size: 429 2269 3507
Fragments arranged by size:
3507 2269 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2779 4700 4874 65
Size: 1183 1531 1921 174 1396
Fragments arranged by size:
1921 1531 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2580 4849 5036 1700
Size: 110 770 2269 187 2869
Fragments arranged by size:
2869 2269 770 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6205
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 4265 5277 4265
Size: 1012 5193
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6205
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 2447 1184
Size: 1263 4942
BstYI r'GATC_y
Cuts at: 1972 2909 3157 3388 3397 3535 3902 4287 4304
Size: 937 248 231 9 138 367 385 17
Cuts at: 4304 5756 5842 5854 6200 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 937 385 367 346 248 231
138 86 17 12 9
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4714
Fragments arranged by size:
4714 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5466
Fragments arranged by size:
5466 683 56
BtsI GCAGTG_nn'
Cuts at: 3721 4515 4535 3721
Size: 794 20 5391
Fragments arranged by size:
5391 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 2413 3285 3509 2413
Size: 872 224 5109
Fragments arranged by size:
5109 872 224
DraIII CAC_nnn'GTG
Cuts at: 2617 2617
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5212 809
Size: 507 3896 1802
Fragments arranged by size:
3896 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3652 4541
Size: 54 1425 50 275 18 177 1645 889
Cuts at: 4541 6170 8
Size: 1629 43
Fragments arranged by size:
1645 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3652 1537
Size: 275 1840 4090
Fragments arranged by size:
4090 1840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2699 3115 4135 1353
Size: 1346 416 1020 3423
Fragments arranged by size:
3423 1346 1020 416
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4792 5166 5312 387
Size: 1064 138 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2800 2904 2800
Size: 104 6101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2345 2522 2747 3377 4268 4865
Size: 393 414 299 177 225 630 891 597
Cuts at: 4865 5652 5920 1239
Size: 787 268 1524
Fragments arranged by size:
1524 891 787 630 597 414 393 299
268 225 177
EcoO109I rG'GnC_Cy
Cuts at: 2184 3171 2184
Size: 987 5218
EcoRI G'AATT_C
Cuts at: 3514 3892 3514
Size: 378 5827
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 5352 1678
Size: 3674 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2698 2730 2822 2854 4846
Size: 32 135 32 1126 32 92 32 1992
Cuts at: 4846 4878 4920 4952 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1992 1126 135 92 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3549 3795 669
Size: 988 281 1611 246 3079
Fragments arranged by size:
3079 1611 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2402 3495 748
Size: 1654 1093 3458
Fragments arranged by size:
3458 1654 1093
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3135 5319 5503 1305
Size: 1830 2184 184 2007
Fragments arranged by size:
2184 2007 1830 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4206
Fragments arranged by size:
4206 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2618 4163 4522 4681 518
Size: 648 1326 126 1545 359 159 2042
Fragments arranged by size:
2042 1545 1326 648 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 3621 4298 5446 5691 1606
Size: 2015 677 1148 245 2120
Fragments arranged by size:
2120 2015 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4714
Fragments arranged by size:
4714 939 552
NdeI CA'TA_TG
Cuts at: 387 2697 3025 387
Size: 2310 328 3567
Fragments arranged by size:
3567 2310 328
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 2435 2955 3563 5108 1132
Size: 97 1206 520 608 1545 2229
Fragments arranged by size:
2229 1545 1206 608 520 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6205
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6205
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3171 3171
Size: 6205
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PstI C_TGCA'G
Cuts at: 830 3557 830
Size: 2727 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
SacI G_AGCT'C
Cuts at: 2802 2906 2802
Size: 104 6101
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SanDI GG'GwC_CC
Cuts at: 3171 3171
Size: 6205
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SfcI C'TryA_G
Cuts at: 826 1045 3411 3553 4688 5369 5560 826
Size: 219 2366 142 1135 681 191 1471
Fragments arranged by size:
2366 1471 1135 681 219 191 142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 3532 1872
Size: 1660 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3126 4309 5210 5472 820
Size: 197 926 1183 1183 901 262 1553
Fragments arranged by size:
1553 1183 1183 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 2435 3563 1132
Size: 1303 1128 3774
Fragments arranged by size:
3774 1303 1128
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 2129 2219 1517
Size: 612 90 5503
Fragments arranged by size:
5503 612 90
StyI C'CwwG_G
Cuts at: 513 1065 2004 2214 3322 3644 513
Size: 552 939 210 1108 322 3074
Fragments arranged by size:
3074 1108 939 552 322 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4232 4249 4402 4587 5032 325
Size: 1255 2652 17 153 185 445 1498
Fragments arranged by size:
2652 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2232 4451 6163
Size: 274 80 33 51 1443 255 2219 1712
Cuts at: 6163 96
Size: 138
Fragments arranged by size:
2219 1712 1443 274 255 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2002 2034 2962 2994 6165 6197 2002
Size: 32 928 32 3171 32 2010
Fragments arranged by size:
3171 2010 928 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6205
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 3530 1870
Size: 1660 4545
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI