Restriction map for
pFN21AB3797
(Circular) (Six-base) MAPSORT of: pFN21AB3797.seq Check: 9181 from: 1 to: 6127
pFN21AB3797 6127 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3470 1937
Size: 1533 4594
Acc65I G'GTAC_C
Cuts at: 2502 3448 2502
Size: 946 5181
AclI AA'CG_TT
Cuts at: 1563 3426 4254 4627 1563
Size: 1863 828 373 3063
Fragments arranged by size:
3063 1863 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 2799 4190 5574 5842 1632
Size: 618 549 1391 1384 268 1917
Fragments arranged by size:
1917 1391 1384 618 549 268
AflII C'TTAA_G
Cuts at: 820 1017 2717 820
Size: 197 1700 4230
Fragments arranged by size:
4230 1700 197
AflIII A'CryG_T
Cuts at: 1203 2922 3265 5026 6003 1203
Size: 1719 343 1761 977 1327
Fragments arranged by size:
1761 1719 1327 977 343
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6127
AlwNI CAG_nnn'CTG
Cuts at: 3870 5442 3870
Size: 1572 4555
ApaI G_GGCC'C
Cuts at: 3009 3009
Size: 6127
ApaLI G'TGCA_C
Cuts at: 2393 4184 5340 2393
Size: 1791 1156 3180
Fragments arranged by size:
3180 1791 1156
ApoI r'AATT_y
Cuts at: 1493 3436 3662 3814 5923 1493
Size: 1943 226 152 2109 1697
Fragments arranged by size:
2109 1943 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2802 4681 160
Size: 872 1770 1879 1606
Fragments arranged by size:
1879 1770 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 3116 3452 1870
Size: 73 1173 336 4545
Fragments arranged by size:
4545 1173 336 73
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2353 2441 2502 3448 4903
Size: 325 217 627 566 88 61 946 1455
Cuts at: 4903 618
Size: 1842
Fragments arranged by size:
1842 1455 946 627 566 325 217 88
61
BanII G_rGCy'C
Cuts at: 1391 3009 3446 1391
Size: 1618 437 4072
Fragments arranged by size:
4072 1618 437
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2938 928
Size: 971 1039 4117
Fragments arranged by size:
4117 1039 971
BbvCI CC'TCA_GC
Cuts at: 2705 2705
Size: 6127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2576 2610 4318 4352 1903
Size: 34 639 34 1708 34 3678
Fragments arranged by size:
3678 1708 639 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2543 4019 5229 2543
Size: 1476 1210 3441
Fragments arranged by size:
3441 1476 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 2397 3009 4188 5344 1790
Size: 607 612 1179 1156 2573
Fragments arranged by size:
2573 1179 1156 612 607
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3371 3858 4816
Size: 416 53 83 399 24 1954 487 958
Cuts at: 4816 442
Size: 1753
Fragments arranged by size:
1954 1753 958 487 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3188 1056
Size: 2132 3995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2296 3184 4787 1239
Size: 1057 888 1603 2579
Fragments arranged by size:
2579 1603 1057 888
Bpu10I CC'TnA_GC
Cuts at: 2705 2705
Size: 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4252 5117 5415 4252
Size: 865 298 4964
Fragments arranged by size:
4964 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2152 2794 3159 4790 882
Size: 617 653 642 365 1631 2219
Fragments arranged by size:
2219 1631 653 642 617 365
BsaAI yAC'GTr
Cuts at: 493 1204 3918 493
Size: 711 2714 2702
Fragments arranged by size:
2714 2702 711
BsaBI GATnn'nnATC
Cuts at: 2774 6121 2774
Size: 3347 2780
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4316 4951 275
Size: 53 83 186 936 2401 382 635 1451
Fragments arranged by size:
2401 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4560 4958 5232 5379 5790 4560
Size: 398 274 147 411 4897
Fragments arranged by size:
4897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2391 2421 1178
Size: 30 165 30 988 30 4884
Fragments arranged by size:
4884 988 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3307 1186
Size: 2121 4006
BseYI C'CCAG_C
Cuts at: 3221 5330 5826 3221
Size: 2109 496 3522
Fragments arranged by size:
3522 2109 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2509 1163
Size: 573 773 4781
Fragments arranged by size:
4781 773 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2397 3113 4188 4273 5344 2397
Size: 716 1075 85 1071 3180
Fragments arranged by size:
3180 1075 1071 716 85
BsmI GAATG_Cn'
Cuts at: 3349 3638 3731 3349
Size: 289 93 5745
Fragments arranged by size:
5745 289 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2111 3289 2111
Size: 1178 4949
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2397 3009 3113 3446 4188 4273 5344
Size: 399 607 612 104 333 742 85 1071
Cuts at: 5344 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 612 607 399 333 104
85
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3482 844
Size: 2638 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4014 1394
Size: 429 2191 3507
Fragments arranged by size:
3507 2191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2205 4622 4796 65
Size: 1183 957 2417 174 1396
Fragments arranged by size:
2417 1396 1183 957 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4771 4958 1700
Size: 110 2961 187 2869
Fragments arranged by size:
2961 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 4187 5199 4187
Size: 1012 5115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6127
BstBI TT'CG_AA
Cuts at: 4931 4931
Size: 6127
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstYI r'GATC_y
Cuts at: 1972 3457 3824 4209 4226 5678 5764 5776 6122
Size: 1485 367 385 17 1452 86 12 346
Cuts at: 6122 1972
Size: 1977
Fragments arranged by size:
1977 1485 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2495 3290 2495
Size: 795 5332
BtgI C'CryG_G
Cuts at: 513 1065 2181 513
Size: 552 1116 4459
Fragments arranged by size:
4459 1116 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2601 487
Size: 683 56 1375 4013
Fragments arranged by size:
4013 1375 683 56
BtsI GCAGTG_nn'
Cuts at: 3643 4437 4457 3643
Size: 794 20 5313
Fragments arranged by size:
5313 794 20
ClaI AT'CG_AT
Cuts at: 1554 2217 1554
Size: 663 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 2313 3431 2313
Size: 1118 5009
DraIII CAC_nnn'GTG
Cuts at: 2401 2401
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3135 3244 3574
Size: 54 1425 50 275 18 1305 109 330
Cuts at: 3574 4463 6092 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1305 889 330 275 109 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4057 1353
Size: 2704 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4714 5088 5234 387
Size: 1064 138 3125 374 146 1280
Fragments arranged by size:
3125 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 2296 2799 3184 4190 4787 5574
Size: 393 618 46 503 385 1006 597 787
Cuts at: 5574 5842 1239
Size: 268 1524
Fragments arranged by size:
1524 1006 787 618 597 503 393 385
268 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2692 2692
Size: 6127
EcoO109I rG'GnC_Cy
Cuts at: 2174 3005 3006 2174
Size: 831 1 5295
Fragments arranged by size:
5295 831 1
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
FspI TGC'GCA
Cuts at: 2076 4633 2076
Size: 2557 3570
HaeII r_GCGC'y
Cuts at: 1678 5274 1678
Size: 3596 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 2448 2480 3344
Size: 32 135 32 797 32 47 32 864
Cuts at: 3344 3376 4768 4800 4842 4874 1373
Size: 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1392 864 797 135 47 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2983 3076 3471 3717 669
Size: 988 281 1045 93 395 246 3079
Fragments arranged by size:
3079 1045 988 395 281 246 93
HindIII A'AGCT_T
Cuts at: 748 2714 748
Size: 1966 4161
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 2506 3452 2506
Size: 946 5181
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5241 5425 1305
Size: 3936 184 2007
Fragments arranged by size:
3936 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3137 10
Size: 54 1425 343 1305 3000
Fragments arranged by size:
3000 1425 1305 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 2436 2921 4085 4444 4603 518
Size: 648 1053 217 485 1164 359 159 2042
Fragments arranged by size:
2042 1164 1053 648 485 359 217 159
MspA1I CmG'CkG
Cuts at: 1606 2066 2477 2819 3029 3543 4220 5368 5613
Size: 460 411 342 210 514 677 1148 245
Cuts at: 5613 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 514 460 411 342 245
210
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5575
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 3184 1052
Size: 2132 3995
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2758 2926 3269 3485 5030 1132
Size: 97 1529 168 343 216 1545 2229
Fragments arranged by size:
2229 1545 1529 343 216 168 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 2922 3265 5026 2922
Size: 343 1761 4023
Fragments arranged by size:
4023 1761 343
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2168 1108
Size: 1060 5067
PfoI T'CCnGG_A
Cuts at: 2880 2880
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2174 2174
Size: 6127
PshAI GACnn'nnGTC
Cuts at: 1613 2151 1613
Size: 538 5589
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PspOMI G'GGCC_C
Cuts at: 3005 3005
Size: 6127
PstI C_TGCA'G
Cuts at: 830 3479 830
Size: 2649 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2477 2819 3029 2477
Size: 342 210 5575
Fragments arranged by size:
5575 342 210
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SfcI C'TryA_G
Cuts at: 826 1045 3475 4610 5291 5482 826
Size: 219 2430 1135 681 191 1471
Fragments arranged by size:
2430 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2717 3116 4231 5132 5394 820
Size: 197 926 774 399 1115 901 262 1553
Fragments arranged by size:
1553 1115 926 901 774 399 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2529 3566 513
Size: 552 1464 1037 3074
Fragments arranged by size:
3074 1464 1037 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2341 2888 4154 4171 4324 4509 4954
Size: 1255 761 547 1266 17 153 185 445
Cuts at: 4954 325
Size: 1498
Fragments arranged by size:
1498 1266 1255 761 547 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 4373 6085
Size: 274 80 33 51 1443 282 2114 1712
Cuts at: 6085 96
Size: 138
Fragments arranged by size:
2114 1712 1443 282 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6087 6119 6087
Size: 32 6095
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2218 2218
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 3116 1943
Size: 1173 4954
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI