Restriction map for
pFN21AB3788
(Circular) (Six-base) MAPSORT of: pFN21AB3788.seq Check: 6765 from: 1 to: 6847
pFN21AB3788 6847 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4657 278
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3245 4190 1937
Size: 1308 945 4594
Fragments arranged by size:
4594 1308 945
Acc65I G'GTAC_C
Cuts at: 4168 4168
Size: 6847
AclI AA'CG_TT
Cuts at: 1563 4974 5347 1563
Size: 3411 373 3063
Fragments arranged by size:
3411 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2358 2573 2881 3736 4910 6294 6562 1632
Size: 726 215 308 855 1174 1384 268 1917
Fragments arranged by size:
1917 1384 1174 855 726 308 268 215
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6650
AflIII A'CryG_T
Cuts at: 1203 5746 6723 1203
Size: 4543 977 1327
Fragments arranged by size:
4543 1327 977
AgeI A'CCGG_T
Cuts at: 5678 5678
Size: 6847
AhdI GACnn_n'nnGTC
Cuts at: 5576 5576
Size: 6847
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6847
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6847
AlwNI CAG_nnn'CTG
Cuts at: 2077 4590 6162 2077
Size: 2513 1572 2762
Fragments arranged by size:
2762 2513 1572
ApaLI G'TGCA_C
Cuts at: 4904 6060 4904
Size: 1156 5691
ApoI r'AATT_y
Cuts at: 1493 2219 2364 2431 2540 2743 2828 3311 3409
Size: 726 145 67 109 203 85 483 98
Cuts at: 3409 3571 3809 3849 4064 4101 4156 4382 4534
Size: 162 238 40 215 37 55 226 152
Cuts at: 4534 6643 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 726 483 238 226 215 203
162 152 145 109 98 85 67 55
40 37
AseI AT'TA_AT
Cuts at: 160 1032 2453 5401 160
Size: 872 1421 2948 1606
Fragments arranged by size:
2948 1606 1421 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
AvaI C'yCGr_G
Cuts at: 1870 1943 3321 4172 1870
Size: 73 1378 851 4545
Fragments arranged by size:
4545 1378 851 73
BamHI G'GATC_C
Cuts at: 4177 4544 4177
Size: 367 6480
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3588 4168 5623 618
Size: 325 217 627 1801 580 1455 1842
Fragments arranged by size:
1842 1801 1455 627 580 325 217
BanII G_rGCy'C
Cuts at: 1391 2851 4166 1391
Size: 1460 1315 4072
Fragments arranged by size:
4072 1460 1315
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2295 3889 928
Size: 971 396 1594 3886
Fragments arranged by size:
3886 1594 971 396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5038 5072 1903
Size: 34 3101 34 3678
Fragments arranged by size:
3678 3101 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2551 4739 5949 2551
Size: 2188 1210 3449
Fragments arranged by size:
3449 2188 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6847
BfrBI ATG'CAT
Cuts at: 2634 2753 2634
Size: 119 6728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5458 136
Size: 107 122 71 631 605 3786 1525
Fragments arranged by size:
3786 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6842 6842
Size: 6847
BlpI GC'TnA_GC
Cuts at: 4264 4264
Size: 6847
Bme1580I G_kGCm'C
Cuts at: 1790 3591 4908 6064 1790
Size: 1801 1317 1156 2573
Fragments arranged by size:
2573 1801 1317 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6847
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4578 5536 442
Size: 416 53 83 399 24 3161 958 1753
Fragments arranged by size:
3161 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2269 5507 1239
Size: 1030 3238 2579
Fragments arranged by size:
3238 2579 1030
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4972 5837 6135 4972
Size: 865 298 5684
Fragments arranged by size:
5684 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5510 882
Size: 617 4011 2219
Fragments arranged by size:
4011 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4638 493
Size: 711 3434 2702
Fragments arranged by size:
3434 2702 711
BsaBI GATnn'nnATC
Cuts at: 6841 6841
Size: 6847
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4654 5036 5671 275
Size: 53 83 186 936 3121 382 635 1451
Fragments arranged by size:
3121 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5280 5678 5952 6099 6510 5280
Size: 398 274 147 411 5617
Fragments arranged by size:
5617 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6622
Fragments arranged by size:
6622 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2210 2957 1186
Size: 892 132 747 5076
Fragments arranged by size:
5076 892 747 132
BseYI C'CCAG_C
Cuts at: 6050 6546 6050
Size: 496 6351
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3074 1163
Size: 573 1338 4936
Fragments arranged by size:
4936 1338 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4297 5058 6086 1540
Size: 275 186 2296 761 1028 2301
Fragments arranged by size:
2301 2296 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4908 4993 6064 4908
Size: 85 1071 5691
Fragments arranged by size:
5691 1071 85
BsmI GAATG_Cn'
Cuts at: 2636 2795 4358 4451 2636
Size: 159 1563 93 5032
Fragments arranged by size:
5032 1563 159 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2851 3591 4166 4908 4993 6064 1391
Size: 399 1061 740 575 742 85 1071 2174
Fragments arranged by size:
2174 1071 1061 742 740 575 399 85
BspEI T'CCGG_A
Cuts at: 6510 6510
Size: 6847
BspHI T'CATG_A
Cuts at: 4736 6466 4736
Size: 1730 5117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4202 844
Size: 3358 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4734 1394
Size: 429 2911 3507
Fragments arranged by size:
3507 2911 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2012 5342 5516 65
Size: 1183 764 3330 174 1396
Fragments arranged by size:
3330 1396 1183 764 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5491 5678 1700
Size: 110 3681 187 2869
Fragments arranged by size:
3681 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2920 4006 96
Size: 2824 1086 2937
Fragments arranged by size:
2937 2824 1086
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6847
BssSI C'ACGA_G
Cuts at: 4907 5919 4907
Size: 1012 5835
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6847
BstBI TT'CG_AA
Cuts at: 5651 5651
Size: 6847
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6847
BstXI CCAn_nnnn'nTGG
Cuts at: 2247 2247
Size: 6847
BstYI r'GATC_y
Cuts at: 1972 4177 4544 4929 4946 6398 6484 6496 6842
Size: 2205 367 385 17 1452 86 12 346
Cuts at: 6842 1972
Size: 1977
Fragments arranged by size:
2205 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2201 513
Size: 552 1136 5159
Fragments arranged by size:
5159 1136 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6108
Fragments arranged by size:
6108 683 56
BtsI GCAGTG_nn'
Cuts at: 2800 4363 5157 5177 2800
Size: 1563 794 20 4470
Fragments arranged by size:
4470 1563 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6847
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6812
DraI TTT'AAA
Cuts at: 3408 4151 3408
Size: 743 6104
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5854 809
Size: 507 4538 1802
Fragments arranged by size:
4538 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4294 5183 6812
Size: 54 1425 50 275 18 2464 889 1629
Cuts at: 6812 8
Size: 43
Fragments arranged by size:
2464 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4294 1537
Size: 275 2482 4090
Fragments arranged by size:
4090 2482 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 3357 3441 3519 3687 3755 4105 4777
Size: 981 1023 84 78 168 68 350 672
Cuts at: 4777 1353
Size: 3423
Fragments arranged by size:
3423 1023 981 672 350 168 84 78
68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5434 5808 5954 387
Size: 1064 138 3845 374 146 1280
Fragments arranged by size:
3845 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2269 2358 2573 2881 3736 4910 5507
Size: 393 637 89 215 308 855 1174 597
Cuts at: 5507 6294 6562 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1174 855 787 637 597 393 308
268 215 89
EcoO109I rG'GnC_Cy
Cuts at: 3117 3117
Size: 6847
EcoRI G'AATT_C
Cuts at: 3849 4101 4156 4534 3849
Size: 252 55 378 6162
Fragments arranged by size:
6162 378 252 55
FspI TGC'GCA
Cuts at: 5353 5353
Size: 6847
HaeII r_GCGC'y
Cuts at: 1678 5994 1678
Size: 4316 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3106 3138 3244 3276 5488
Size: 32 135 32 1534 32 106 32 2212
Cuts at: 5488 5520 5562 5594 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2212 1534 135 106 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2231 4191 4437 669
Size: 988 281 293 1960 246 3079
Fragments arranged by size:
3079 1960 988 293 281 246
HindIII A'AGCT_T
Cuts at: 748 3200 748
Size: 2452 4395
HpaI GTT'AAC
Cuts at: 4437 4437
Size: 6847
KpnI G_GTAC'C
Cuts at: 4172 4172
Size: 6847
MfeI C'AATT_G
Cuts at: 4446 4446
Size: 6847
MluI A'CGCG_T
Cuts at: 6723 6723
Size: 6847
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 2163 5961 6145 1305
Size: 792 66 3798 184 2007
Fragments arranged by size:
3798 2007 792 184 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5025
Fragments arranged by size:
5025 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2412 2735 4805 5164 5323 518
Size: 648 1246 323 2070 359 159 2042
Fragments arranged by size:
2070 2042 1246 648 359 323 159
MspA1I CmG'CkG
Cuts at: 1606 2077 4263 4940 6088 6333 1606
Size: 471 2186 677 1148 245 2120
Fragments arranged by size:
2186 2120 1148 677 471 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6295
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6847
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6847
NotI GC'GGCC_GC
Cuts at: 4294 4294
Size: 6847
NsiI A_TGCA'T
Cuts at: 2636 2755 2636
Size: 119 6728
NspI r_CATG'y
Cuts at: 1132 1229 4205 5750 1132
Size: 97 2976 1545 2229
Fragments arranged by size:
2976 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6847
PciI A'CATG_T
Cuts at: 5746 5746
Size: 6847
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6847
PmeI GTTT'AAAC
Cuts at: 4151 4151
Size: 6847
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4916 4948 6429 6461 4916
Size: 32 1481 32 5302
Fragments arranged by size:
5302 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6847
PsiI TTA'TAA
Cuts at: 3207 4417 3207
Size: 1210 5637
PstI C_TGCA'G
Cuts at: 830 4199 830
Size: 3369 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6847
PvuII CAG'CTG
Cuts at: 2077 2077
Size: 6847
SalI G'TCGA_C
Cuts at: 1936 4189 1936
Size: 2253 4594
SbfI CC_TGCA'GG
Cuts at: 4199 4199
Size: 6847
ScaI AGT'ACT
Cuts at: 5095 6807 5095
Size: 1712 5135
SfcI C'TryA_G
Cuts at: 826 1045 2125 3928 4012 4195 5330 6011 6202
Size: 219 1080 1803 84 183 1135 681 191
Cuts at: 6202 826
Size: 1471
Fragments arranged by size:
1803 1471 1135 1080 681 219 191 183
84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6847
SmaI CCC'GGG
Cuts at: 1872 4174 1872
Size: 2302 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4951 5852 6114 820
Size: 197 926 3008 901 262 1553
Fragments arranged by size:
3008 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4638 493
Size: 4145 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6847
SphI G_CATG'C
Cuts at: 1132 4205 1132
Size: 3073 3774
SspI AAT'ATT
Cuts at: 5 52 2584 4771 5
Size: 47 2532 2187 2081
Fragments arranged by size:
2532 2187 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6847
StyI C'CwwG_G
Cuts at: 513 1065 4286 513
Size: 552 3221 3074
Fragments arranged by size:
3221 3074 552
SwaI ATTT'AAAT
Cuts at: 3408 3408
Size: 6847
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 4874 4891 5044 5229 5674 325
Size: 1255 492 2802 17 153 185 445 1498
Fragments arranged by size:
2802 1498 1255 492 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2920 3436 3766
Size: 274 80 33 51 1443 943 516 330
Cuts at: 3766 4006 4126 5093 6805 96
Size: 240 120 967 1712 138
Fragments arranged by size:
1712 1443 967 943 516 330 274 240
138 120 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6807 6839 6807
Size: 32 6815
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6847
XbaI T'CTAG_A
Cuts at: 4183 4183
Size: 6847
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6847
XmaI C'CCGG_G
Cuts at: 1870 4172 1870
Size: 2302 4545
XmnI GAAnn'nnTTC
Cuts at: 2583 2894 3019 3062 3146 3560 3685 3853 3937
Size: 311 125 43 84 414 125 168 84
Cuts at: 3937 4021 4064 4976 2583
Size: 84 43 912 4454
Fragments arranged by size:
4454 912 414 311 168 125 125 84
84 84 43 43
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4655 276
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI XcmI