Restriction map for
pFN21AB3783
(Circular) (Six-base) MAPSORT of: pFN21AB3783.seq Check: 6826 from: 1 to: 6979
pFN21AB3783 6979 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3615 4023 4789 278
Size: 53 83 186 936 2079 408 766 2468
Fragments arranged by size:
2468 2079 936 766 408 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4322 1937
Size: 2385 4594
Acc65I G'GTAC_C
Cuts at: 4300 4300
Size: 6979
AclI AA'CG_TT
Cuts at: 1563 5106 5479 1563
Size: 3543 373 3063
Fragments arranged by size:
3543 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 2654 3698 3848 5042 6426 6694 1632
Size: 653 369 1044 150 1194 1384 268 1917
Fragments arranged by size:
1917 1384 1194 1044 653 369 268 150
AflII C'TTAA_G
Cuts at: 820 1017 2357 820
Size: 197 1340 5442
Fragments arranged by size:
5442 1340 197
AflIII A'CryG_T
Cuts at: 1203 5878 6855 1203
Size: 4675 977 1327
Fragments arranged by size:
4675 1327 977
AgeI A'CCGG_T
Cuts at: 5810 5810
Size: 6979
AhdI GACnn_n'nnGTC
Cuts at: 2806 4024 5708 2806
Size: 1218 1684 4077
Fragments arranged by size:
4077 1684 1218
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6979
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2549 2581 2549
Size: 32 6947
AlwNI CAG_nnn'CTG
Cuts at: 2023 4060 4722 6294 2023
Size: 2037 662 1572 2708
Fragments arranged by size:
2708 2037 1572 662
ApaI G_GGCC'C
Cuts at: 2103 2103
Size: 6979
ApaLI G'TGCA_C
Cuts at: 3619 4142 5036 6192 3619
Size: 523 894 1156 4406
Fragments arranged by size:
4406 1156 894 523
ApoI r'AATT_y
Cuts at: 1493 4288 4514 4666 6775 1493
Size: 2795 226 152 2109 1697
Fragments arranged by size:
2795 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5533 160
Size: 872 4501 1606
Fragments arranged by size:
4501 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
AvaI C'yCGr_G
Cuts at: 1870 1943 2762 3128 3172 4304 1870
Size: 73 819 366 44 1132 4545
Fragments arranged by size:
4545 1132 819 366 73 44
BamHI G'GATC_C
Cuts at: 2168 2282 3873 4309 4676 2168
Size: 114 1591 436 367 4471
Fragments arranged by size:
4471 1591 436 367 114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2758 4300 5755 618
Size: 325 217 627 971 1542 1455 1842
Fragments arranged by size:
1842 1542 1455 971 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2103 2956 3240 3353 3561 3719 4218 4298
Size: 712 853 284 113 208 158 499 80
Cuts at: 4298 1391
Size: 4072
Fragments arranged by size:
4072 853 712 499 284 208 158 113
80
BbeI G_GCGC'C
Cuts at: 2762 2762
Size: 6979
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2118 928
Size: 971 219 5789
Fragments arranged by size:
5789 971 219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4057 4091 5170 5204 1903
Size: 34 50 34 2036 34 1079 34 3678
Fragments arranged by size:
3678 2036 1079 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4871 6081 4871
Size: 1210 5769
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6979
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5590 136
Size: 107 122 71 631 605 3918 1525
Fragments arranged by size:
3918 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2629 3250 6974 2629
Size: 621 3724 2634
Fragments arranged by size:
3724 2634 621
BlpI GC'TnA_GC
Cuts at: 2577 4127 4239 4396 2577
Size: 1550 112 157 5160
Fragments arranged by size:
5160 1550 157 112
Bme1580I G_kGCm'C
Cuts at: 1790 2103 2670 3623 4146 5040 6196 1790
Size: 313 567 953 523 894 1156 2573
Fragments arranged by size:
2573 1156 953 894 567 523 313
BmgBI CAC'GTC
Cuts at: 1318 4141 4266 1318
Size: 2823 125 4031
Fragments arranged by size:
4031 2823 125
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 3260 4000
Size: 416 53 83 399 24 828 1015 740
Cuts at: 4000 4710 5668 442
Size: 710 958 1753
Fragments arranged by size:
1753 1015 958 828 740 710 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6979
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2531 2531
Size: 6979
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3883 4232 5639 1239
Size: 2644 349 1407 2579
Fragments arranged by size:
2644 2579 1407 349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3205 3479 5104 5969 6267 3205
Size: 274 1625 865 298 3917
Fragments arranged by size:
3917 1625 865 298 274
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2060 2870 3426 3666 5642 882
Size: 617 561 810 556 240 1976 2219
Fragments arranged by size:
2219 1976 810 617 561 556 240
BsaAI yAC'GTr
Cuts at: 493 1204 4042 4770 493
Size: 711 2838 728 2702
Fragments arranged by size:
2838 2702 728 711
BsaBI GATnn'nnATC
Cuts at: 2204 3452 6973 2204
Size: 1248 3521 2210
Fragments arranged by size:
3521 2210 1248
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2187 2759 3146 3165
Size: 53 83 186 936 654 572 387 19
Cuts at: 3165 3612 4020 4786 5168 5803 275
Size: 447 408 766 382 635 1451
Fragments arranged by size:
1451 936 766 654 635 572 447 408
387 382 186 83 53 19
BsaWI w'CCGG_w
Cuts at: 3060 5412 5810 6084 6231 6642 3060
Size: 2352 398 274 147 411 3397
Fragments arranged by size:
3397 2352 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3142 3172 1178
Size: 30 165 30 1739 30 4985
Fragments arranged by size:
4985 1739 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2567 3953 1186
Size: 904 477 1386 4212
Fragments arranged by size:
4212 1386 904 477
BseYI C'CCAG_C
Cuts at: 2330 2905 2983 6182 6678 2330
Size: 575 78 3199 496 2631
Fragments arranged by size:
3199 2631 575 496 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3239 3302 1163
Size: 573 1503 63 4840
Fragments arranged by size:
4840 1503 573 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2748 4429 5190 6218 1540
Size: 275 186 747 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 747 275 186
BsiHKAI G_wGCw'C
Cuts at: 2823 2956 3561 3623 4080 4146 5040 5125 6196
Size: 133 605 62 457 66 894 85 1071
Cuts at: 6196 2823
Size: 3606
Fragments arranged by size:
3606 1071 894 605 457 133 85 66
62
BsmI GAATG_Cn'
Cuts at: 4490 4583 4490
Size: 93 6886
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2670 2823 2956 3240 3353 3561
Size: 399 313 567 153 133 284 113 208
Cuts at: 3561 3623 3719 4080 4146 4218 4298 5040 5125
Size: 62 96 361 66 72 80 742 85
Cuts at: 5125 6196 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 567 399 361 313 284
208 153 133 113 96 85 80 72
66 62
BspEI T'CCGG_A
Cuts at: 6642 6642
Size: 6979
BspHI T'CATG_A
Cuts at: 3783 4868 6598 3783
Size: 1085 1730 4164
Fragments arranged by size:
4164 1730 1085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4334 844
Size: 3490 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4866 1394
Size: 429 3043 3507
Fragments arranged by size:
3507 3043 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5474 5648 65
Size: 1183 4226 174 1396
Fragments arranged by size:
4226 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2775 2979 5623 5810 1700
Size: 110 965 204 2644 187 2869
Fragments arranged by size:
2869 2644 965 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 3696 3882 96
Size: 3600 186 3193
Fragments arranged by size:
3600 3193 186
BssHII G'CGCG_C
Cuts at: 1925 3763 1925
Size: 1838 5141
BssSI C'ACGA_G
Cuts at: 5039 6051 5039
Size: 1012 5967
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3253 794
Size: 2459 4520
BstBI TT'CG_AA
Cuts at: 5783 5783
Size: 6979
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6979
BstXI CCAn_nnnn'nTGG
Cuts at: 3637 3811 4276 3637
Size: 174 465 6340
Fragments arranged by size:
6340 465 174
BstYI r'GATC_y
Cuts at: 1972 2080 2168 2242 2282 2629 3250 3453 3873
Size: 108 88 74 40 347 621 203 420
Cuts at: 3873 4309 4676 5061 5078 6530 6616 6628 6974
Size: 436 367 385 17 1452 86 12 346
Cuts at: 6974 1972
Size: 1977
Fragments arranged by size:
1977 1452 621 436 420 385 367 347
346 203 108 88 86 74 40 17
12
Bsu36I CC'TnA_GG
Cuts at: 3105 3936 4197 3105
Size: 831 261 5887
Fragments arranged by size:
5887 831 261
BtgI C'CryG_G
Cuts at: 513 1065 2880 3387 3636 4088 4205 513
Size: 552 1815 507 249 452 117 3287
Fragments arranged by size:
3287 1815 552 507 452 249 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3385 4082 4175 487
Size: 683 56 2159 697 93 3291
Fragments arranged by size:
3291 2159 697 683 93 56
BtsI GCAGTG_nn'
Cuts at: 2115 3099 3967 4495 5289 5309 2115
Size: 984 868 528 794 20 3785
Fragments arranged by size:
3785 984 868 794 528 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6979
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4159 4194 529
Size: 35 3595 35 3314
Fragments arranged by size:
3595 3314 35 35
DraI TTT'AAA
Cuts at: 4283 4283
Size: 6979
DraIII CAC_nnn'GTG
Cuts at: 4150 4150
Size: 6979
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5986 809
Size: 507 4670 1802
Fragments arranged by size:
4670 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3469 3801 4164
Size: 54 1425 50 275 18 1639 332 363
Cuts at: 4164 4426 5315 6944 8
Size: 262 889 1629 43
Fragments arranged by size:
1639 1629 1425 889 363 332 275 262
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4426 1537
Size: 275 2614 4090
Fragments arranged by size:
4090 2614 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2522 2655 2963 3438 4250 4909 1353
Size: 1169 133 308 475 812 659 3423
Fragments arranged by size:
3423 1169 812 659 475 308 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5566 5940 6086 387
Size: 1064 138 3977 374 146 1280
Fragments arranged by size:
3977 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2954 3559 2954
Size: 605 6374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2654 3698 3848 3883 4232 5042
Size: 393 653 369 1044 150 35 349 810
Cuts at: 5042 5639 6426 6694 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1044 810 787 653 597 393 369
349 268 150 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2696 3230 4151 2696
Size: 534 921 5524
Fragments arranged by size:
5524 921 534
EcoO109I rG'GnC_Cy
Cuts at: 2099 2353 2512 2844 3823 4201 2099
Size: 254 159 332 979 378 4877
Fragments arranged by size:
4877 979 378 332 254 159
EcoRI G'AATT_C
Cuts at: 4288 4666 4288
Size: 378 6601
EcoRV GAT'ATC
Cuts at: 4002 4002
Size: 6979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 6979
FspI TGC'GCA
Cuts at: 5485 5485
Size: 6979
HaeII r_GCGC'y
Cuts at: 1678 2762 3022 4060 6126 1678
Size: 1084 260 1038 2066 2531
Fragments arranged by size:
2531 2066 1084 1038 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2792 2824 2869 2901 3986
Size: 32 135 32 1220 32 45 32 1085
Cuts at: 3986 4010 4018 4042 4097 4129 5620 5652 5694
Size: 24 8 24 55 32 1491 32 42
Cuts at: 5694 5726 1373
Size: 32 2626
Fragments arranged by size:
2626 1491 1220 1085 135 55 45 42
32 32 32 32 32 32 32 24
24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 4099 4323 4569 669
Size: 988 281 2161 224 246 3079
Fragments arranged by size:
3079 2161 988 281 246 224
HindIII A'AGCT_T
Cuts at: 748 2447 748
Size: 1699 5280
HpaI GTT'AAC
Cuts at: 4569 4569
Size: 6979
KasI G'GCGC_C
Cuts at: 2758 2758
Size: 6979
KpnI G_GTAC'C
Cuts at: 4304 4304
Size: 6979
MfeI C'AATT_G
Cuts at: 4578 4578
Size: 6979
MluI A'CGCG_T
Cuts at: 6855 6855
Size: 6979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2349 4254 6093 6277 1305
Size: 1044 1905 1839 184 2007
Fragments arranged by size:
2007 1905 1839 1044 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5157
Fragments arranged by size:
5157 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3344 4937 5296 5455 518
Size: 648 2178 1593 359 159 2042
Fragments arranged by size:
2178 2042 1593 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2144 3760 4207 4395 5072 6220 6465 1606
Size: 538 1616 447 188 677 1148 245 2120
Fragments arranged by size:
2120 1616 1148 677 538 447 245 188
NarI GG'CG_CC
Cuts at: 2759 2759
Size: 6979
NcoI C'CATG_G
Cuts at: 513 1065 2880 3636 513
Size: 552 1815 756 3856
Fragments arranged by size:
3856 1815 756 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6979
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6979
NotI GC'GGCC_GC
Cuts at: 4426 4426
Size: 6979
NspI r_CATG'y
Cuts at: 1132 1229 4337 5882 1132
Size: 97 3108 1545 2229
Fragments arranged by size:
3108 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3655 1348
Size: 2307 4672
PciI A'CATG_T
Cuts at: 5878 5878
Size: 6979
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2611 3045 1108
Size: 1503 434 5042
Fragments arranged by size:
5042 1503 434
PfoI T'CCnGG_A
Cuts at: 2490 2652 3231 3561 2490
Size: 162 579 330 5908
Fragments arranged by size:
5908 579 330 162
PmeI GTTT'AAAC
Cuts at: 4283 4283
Size: 6979
PmlI CAC'GTG
Cuts at: 4042 4042
Size: 6979
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2560 5048 5080 6561 6593 2528
Size: 32 2488 32 1481 32 2914
Fragments arranged by size:
2914 2488 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2353 2512 2844 3823 2353
Size: 159 332 979 5509
Fragments arranged by size:
5509 979 332 159
PshAI GACnn'nnGTC
Cuts at: 1613 4264 1613
Size: 2651 4328
PsiI TTA'TAA
Cuts at: 4549 4549
Size: 6979
PspOMI G'GGCC_C
Cuts at: 2099 2099
Size: 6979
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3041 3073 3041
Size: 32 6947
PstI C_TGCA'G
Cuts at: 830 2122 4331 830
Size: 1292 2209 3478
Fragments arranged by size:
3478 2209 1292
PvuI CG_AT'CG
Cuts at: 2001 2748 2001
Size: 747 6232
PvuII CAG'CTG
Cuts at: 2144 3760 2144
Size: 1616 5363
SacI G_AGCT'C
Cuts at: 2956 3561 2956
Size: 605 6374
SacII CC_GC'GG
Cuts at: 4208 4208
Size: 6979
SalI G'TCGA_C
Cuts at: 1936 4321 1936
Size: 2385 4594
SanDI GG'GwC_CC
Cuts at: 2844 2844
Size: 6979
SbfI CC_TGCA'GG
Cuts at: 4331 4331
Size: 6979
ScaI AGT'ACT
Cuts at: 5227 6939 5227
Size: 1712 5267
SexAI A'CCwGG_T
Cuts at: 3528 4146 3528
Size: 618 6361
SfcI C'TryA_G
Cuts at: 826 1045 2118 2623 4327 5462 6143 6334 826
Size: 219 1073 505 1704 1135 681 191 1471
Fragments arranged by size:
1704 1471 1135 1073 681 505 219 191
SfoI GGC'GCC
Cuts at: 2760 2760
Size: 6979
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6979
SmaI CCC'GGG
Cuts at: 1872 4306 1872
Size: 2434 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2357 3220 3494 5083 5984 6246
Size: 197 926 414 863 274 1589 901 262
Cuts at: 6246 820
Size: 1553
Fragments arranged by size:
1589 1553 926 901 863 414 274 262
197
SnaBI TAC'GTA
Cuts at: 493 4770 493
Size: 4277 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6979
SphI G_CATG'C
Cuts at: 1132 4337 1132
Size: 3205 3774
SspI AAT'ATT
Cuts at: 5 52 4903 5
Size: 47 4851 2081
Fragments arranged by size:
4851 2081 47
StuI AGG'CCT
Cuts at: 1517 3103 1517
Size: 1586 5393
StyI C'CwwG_G
Cuts at: 513 1065 2586 2880 3636 4418 513
Size: 552 1521 294 756 782 3074
Fragments arranged by size:
3074 1521 782 756 552 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2400 2975 3288 4081 5006 5023 5176
Size: 1255 820 575 313 793 925 17 153
Cuts at: 5176 5361 5806 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 925 820 793 575 445 313
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2751 3696 3882
Size: 274 80 33 51 1443 774 945 186
Cuts at: 3882 5225 6937 96
Size: 1343 1712 138
Fragments arranged by size:
1712 1443 1343 945 774 274 186 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3142 3174 3946 3978 6939 6971 3142
Size: 32 772 32 2961 32 3150
Fragments arranged by size:
3150 2961 772 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6979
XbaI T'CTAG_A
Cuts at: 4315 4315
Size: 6979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3314 3314
Size: 6979
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6979
XmaI C'CCGG_G
Cuts at: 1870 4304 1870
Size: 2434 4545
XmnI GAAnn'nnTTC
Cuts at: 5108 5108
Size: 6979
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3613 4021 4787 276
Size: 53 83 186 936 2079 408 766 2468
Fragments arranged by size:
2468 2079 936 766 408 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI RsrII SapI SfiI SrfI SwaI