Restriction map for
pFN21AB3767
(Circular) (Six-base) MAPSORT of: pFN21AB3767.seq Check: 9765 from: 1 to: 7984
pFN21AB3767 7984 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5794 278
Size: 53 83 186 936 4258 2468
Fragments arranged by size:
4258 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2645 3936 5327 1937
Size: 708 1291 1391 4594
Fragments arranged by size:
4594 1391 1291 708
Acc65I G'GTAC_C
Cuts at: 4599 5305 4599
Size: 706 7278
AclI AA'CG_TT
Cuts at: 1563 6111 6484 1563
Size: 4548 373 3063
Fragments arranged by size:
4548 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2278 2544 3144 3156 3914 4226 5079 6047
Size: 646 266 600 12 758 312 853 968
Cuts at: 6047 7431 7699 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 968 853 758 646 600 312
268 266 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7787
AflIII A'CryG_T
Cuts at: 1203 6883 7860 1203
Size: 5680 977 1327
Fragments arranged by size:
5680 1327 977
AgeI A'CCGG_T
Cuts at: 6815 6815
Size: 7984
AhdI GACnn_n'nnGTC
Cuts at: 6713 6713
Size: 7984
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7984
AlwNI CAG_nnn'CTG
Cuts at: 2053 3712 4282 5727 7299 2053
Size: 1659 570 1445 1572 2738
Fragments arranged by size:
2738 1659 1572 1445 570
ApaLI G'TGCA_C
Cuts at: 6041 7197 6041
Size: 1156 6828
ApoI r'AATT_y
Cuts at: 1493 2287 3359 3889 4333 4479 4507 5293 5519
Size: 794 1072 530 444 146 28 786 226
Cuts at: 5519 5671 7780 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1072 794 786 530 444 226
152 146 28
AseI AT'TA_AT
Cuts at: 160 1032 6538 160
Size: 872 5506 1606
Fragments arranged by size:
5506 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7984
AvaI C'yCGr_G
Cuts at: 1870 1943 2827 5309 1870
Size: 73 884 2482 4545
Fragments arranged by size:
4545 2482 884 73
BamHI G'GATC_C
Cuts at: 4352 5314 5681 4352
Size: 962 367 6655
Fragments arranged by size:
6655 962 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2569 4599 5305 6760 618
Size: 325 217 627 782 2030 706 1455 1842
Fragments arranged by size:
2030 1842 1455 782 706 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2819 2828 5303 1391
Size: 1428 9 2475 4072
Fragments arranged by size:
4072 2475 1428 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2715 2852 3920 4482 928
Size: 971 816 137 1068 562 4430
Fragments arranged by size:
4430 1068 971 816 562 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6175 6209 1903
Size: 34 4238 34 3678
Fragments arranged by size:
4238 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2170 5876 7086 2170
Size: 3706 1210 3068
Fragments arranged by size:
3706 3068 1210
BclI T'GATC_A
Cuts at: 1548 4876 1548
Size: 3328 4656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6595 136
Size: 107 122 71 631 605 4923 1525
Fragments arranged by size:
4923 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3916 5218 7979 3916
Size: 1302 2761 3921
Fragments arranged by size:
3921 2761 1302
BlpI GC'TnA_GC
Cuts at: 2043 2818 3288 5401 2043
Size: 775 470 2113 4626
Fragments arranged by size:
4626 2113 775 470
Bme1580I G_kGCm'C
Cuts at: 1790 6045 7201 1790
Size: 4255 1156 2573
Fragments arranged by size:
4255 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7984
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5715 6673 442
Size: 416 53 83 399 24 4298 958 1753
Fragments arranged by size:
4298 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2017 2343 4238 4601 6644 1239
Size: 778 326 1895 363 2043 2579
Fragments arranged by size:
2579 2043 1895 778 363 326
Bpu10I CC'TnA_GC
Cuts at: 4988 4988
Size: 7984
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4725 4792 6109 6974 7272 4725
Size: 67 1317 865 298 5437
Fragments arranged by size:
5437 1317 865 298 67
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2117 6647 882
Size: 617 618 4530 2219
Fragments arranged by size:
4530 2219 618 617
BsaAI yAC'GTr
Cuts at: 493 1204 5775 493
Size: 711 4571 2702
Fragments arranged by size:
4571 2702 711
BsaBI GATnn'nnATC
Cuts at: 5148 7978 5148
Size: 2830 5154
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5791 6173 6808 275
Size: 53 83 186 936 4258 382 635 1451
Fragments arranged by size:
4258 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6417 6815 7089 7236 7647 6417
Size: 398 274 147 411 6754
Fragments arranged by size:
6754 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2716 2746 3007 3037 3380
Size: 30 165 30 1313 30 261 30 343
Cuts at: 3380 3410 1178
Size: 30 5752
Fragments arranged by size:
5752 1313 343 261 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2724 3824 1186
Size: 931 607 1100 5346
Fragments arranged by size:
5346 1100 931 607
BseYI C'CCAG_C
Cuts at: 2025 4545 7187 7683 2025
Size: 2520 2642 496 2326
Fragments arranged by size:
2642 2520 2326 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3698 4470 1163
Size: 573 1962 772 4677
Fragments arranged by size:
4677 1962 772 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5434 6195 7223 1540
Size: 275 186 3433 761 1028 2301
Fragments arranged by size:
3433 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2819 2828 6045 6130 7201 2819
Size: 9 3217 85 1071 3602
Fragments arranged by size:
3602 3217 1071 85 9
BsmI GAATG_Cn'
Cuts at: 5495 5588 5495
Size: 93 7891
BsmBI CGTCTCn'nnnn_
Cuts at: 4646 4646
Size: 7984
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2819 2828 5303 6045 6130 7201 1391
Size: 399 1029 9 2475 742 85 1071 2174
Fragments arranged by size:
2475 2174 1071 1029 742 399 85 9
BspEI T'CCGG_A
Cuts at: 7647 7647
Size: 7984
BspHI T'CATG_A
Cuts at: 2794 4873 5873 7603 2794
Size: 2079 1000 1730 3175
Fragments arranged by size:
3175 2079 1730 1000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2492 3310 5339 844
Size: 1648 818 2029 3489
Fragments arranged by size:
3489 2029 1648 818
BsrBI CCG'CTC
Cuts at: 1394 1823 5871 1394
Size: 429 4048 3507
Fragments arranged by size:
4048 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4313 6479 6653 65
Size: 1183 2927 138 2166 174 1396
Fragments arranged by size:
2927 2166 1396 1183 174 138
BsrFI r'CCGG_y
Cuts at: 1700 1810 6628 6815 1700
Size: 110 4818 187 2869
Fragments arranged by size:
4818 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2995 5241 96
Size: 2899 2246 2839
Fragments arranged by size:
2899 2839 2246
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7984
BssSI C'ACGA_G
Cuts at: 6044 7056 6044
Size: 1012 6972
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7984
BstBI TT'CG_AA
Cuts at: 6788 6788
Size: 7984
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7984
BstXI CCAn_nnnn'nTGG
Cuts at: 4156 4156
Size: 7984
BstYI r'GATC_y
Cuts at: 1972 3666 3916 4352 5218 5314 5681 6066 6083
Size: 1694 250 436 866 96 367 385 17
Cuts at: 6083 7535 7621 7633 7979 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1694 1452 866 436 385 367 346
250 96 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2691 4166 2691
Size: 1475 6509
BtgI C'CryG_G
Cuts at: 513 1065 3894 4494 513
Size: 552 2829 600 4003
Fragments arranged by size:
4003 2829 600 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7245
Fragments arranged by size:
7245 683 56
BtsI GCAGTG_nn'
Cuts at: 3299 5500 6294 6314 3299
Size: 2201 794 20 4969
Fragments arranged by size:
4969 2201 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7984
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7949
DraI TTT'AAA
Cuts at: 5288 5288
Size: 7984
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6991 809
Size: 507 5675 1802
Fragments arranged by size:
5675 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2422 3922 5431
Size: 54 1425 50 275 18 592 1500 1509
Cuts at: 5431 6320 7949 8
Size: 889 1629 43
Fragments arranged by size:
1629 1509 1500 1425 889 592 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5431 1537
Size: 275 3619 4090
Fragments arranged by size:
4090 3619 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2330 2473 3801 3843 4644 5094 5190
Size: 741 236 143 1328 42 801 450 96
Cuts at: 5190 5914 1353
Size: 724 3423
Fragments arranged by size:
3423 1328 801 741 724 450 236 143
96 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6571 6945 7091 387
Size: 1064 138 4982 374 146 1280
Fragments arranged by size:
4982 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2817 2826 2817
Size: 9 7975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2017 2278 2343 2544 3144 3156 3914
Size: 393 385 261 65 201 600 12 758
Cuts at: 3914 4226 4238 4601 5079 6047 6644 7431 7699
Size: 312 12 363 478 968 597 787 268
Cuts at: 7699 1239
Size: 1524
Fragments arranged by size:
1524 968 787 758 600 597 478 393
385 363 312 268 261 201 65 12
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2057
Size: 7984
EcoO109I rG'GnC_Cy
Cuts at: 2050 3832 4162 2050
Size: 1782 330 5872
Fragments arranged by size:
5872 1782 330
EcoRI G'AATT_C
Cuts at: 4507 5293 5671 4507
Size: 786 378 6820
Fragments arranged by size:
6820 786 378
EcoRV GAT'ATC
Cuts at: 3232 3232
Size: 7984
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3623 4106 4484 3623
Size: 483 378 7123
Fragments arranged by size:
7123 483 378
FspI TGC'GCA
Cuts at: 6490 6490
Size: 7984
HaeII r_GCGC'y
Cuts at: 1678 7131 1678
Size: 5453 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2837 2869 3032 3064 5205
Size: 32 135 32 1265 32 163 32 2141
Cuts at: 5205 5237 5262 5294 6625 6657 6699 6731 1373
Size: 32 25 32 1331 32 42 32 2626
Fragments arranged by size:
2626 2141 1331 1265 163 135 42 32
32 32 32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3214 5328 5574 669
Size: 988 281 1276 2114 246 3079
Fragments arranged by size:
3079 2114 1276 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3435 748
Size: 2687 5297
HpaI GTT'AAC
Cuts at: 3214 5574 3214
Size: 2360 5624
KpnI G_GTAC'C
Cuts at: 4603 5309 4603
Size: 706 7278
MfeI C'AATT_G
Cuts at: 5583 5583
Size: 7984
MluI A'CGCG_T
Cuts at: 7860 7860
Size: 7984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7098 7282 1305
Size: 5793 184 2007
Fragments arranged by size:
5793 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2424 10
Size: 54 1425 343 592 5570
Fragments arranged by size:
5570 1425 592 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2799 4154 4366 5942 6301 6460 518
Size: 648 1633 1355 212 1576 359 159 2042
Fragments arranged by size:
2042 1633 1576 1355 648 359 212 159
MspA1I CmG'CkG
Cuts at: 1606 3712 5400 6077 7225 7470 1606
Size: 2106 1688 677 1148 245 2120
Fragments arranged by size:
2120 2106 1688 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3894 4494 513
Size: 552 2829 600 4003
Fragments arranged by size:
4003 2829 600 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7984
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7984
NotI GC'GGCC_GC
Cuts at: 5431 5431
Size: 7984
NspI r_CATG'y
Cuts at: 1132 1229 5342 6887 1132
Size: 97 4113 1545 2229
Fragments arranged by size:
4113 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4348 1348
Size: 3000 4984
PciI A'CATG_T
Cuts at: 6883 6883
Size: 7984
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 7082
PfoI T'CCnGG_A
Cuts at: 2094 4254 2094
Size: 2160 5824
PmeI GTTT'AAAC
Cuts at: 5288 5288
Size: 7984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2973 6053 6085 7566 7598 2941
Size: 32 3080 32 1481 32 3327
Fragments arranged by size:
3327 3080 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3832 4162 3832
Size: 330 7654
PshAI GACnn'nnGTC
Cuts at: 1613 2270 1613
Size: 657 7327
PsiI TTA'TAA
Cuts at: 5554 5554
Size: 7984
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4583 4615 4583
Size: 32 7952
PstI C_TGCA'G
Cuts at: 830 3640 4099 5175 5336 830
Size: 2810 459 1076 161 3478
Fragments arranged by size:
3478 2810 1076 459 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7984
PvuII CAG'CTG
Cuts at: 3712 3712
Size: 7984
SacI G_AGCT'C
Cuts at: 2819 2828 2819
Size: 9 7975
SalI G'TCGA_C
Cuts at: 1936 5326 1936
Size: 3390 4594
SbfI CC_TGCA'GG
Cuts at: 5336 5336
Size: 7984
ScaI AGT'ACT
Cuts at: 6232 7944 6232
Size: 1712 6272
SexAI A'CCwGG_T
Cuts at: 4602 4602
Size: 7984
SfcI C'TryA_G
Cuts at: 826 1045 2841 3636 4095 4455 4841 4970 5171
Size: 219 1796 795 459 360 386 129 201
Cuts at: 5171 5332 6467 7148 7339 826
Size: 161 1135 681 191 1471
Fragments arranged by size:
1796 1471 1135 795 681 459 386 360
219 201 191 161 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7984
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7984
SmaI CCC'GGG
Cuts at: 1872 5311 1872
Size: 3439 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2827 4740 4807 6088 6989 7251
Size: 197 926 884 1913 67 1281 901 262
Cuts at: 7251 820
Size: 1553
Fragments arranged by size:
1913 1553 1281 926 901 884 262 197
67
SnaBI TAC'GTA
Cuts at: 493 5775 493
Size: 5282 2702
SpeI A'CTAG_T
Cuts at: 152 5065 152
Size: 4913 3071
SphI G_CATG'C
Cuts at: 1132 5342 1132
Size: 4210 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5908 5
Size: 47 3825 2031 2081
Fragments arranged by size:
3825 2081 2031 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7984
StyI C'CwwG_G
Cuts at: 513 1065 3894 4494 5423 513
Size: 552 2829 600 929 3074
Fragments arranged by size:
3074 2829 929 600 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6011 6028 6181 6366 6811 325
Size: 1255 4431 17 153 185 445 1498
Fragments arranged by size:
4431 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2995 5241 6230
Size: 274 80 33 51 1443 1018 2246 989
Cuts at: 6230 7942 96
Size: 1712 138
Fragments arranged by size:
2246 1712 1443 1018 989 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3007 3039 3255 3287 7944 7976 3007
Size: 32 216 32 4657 32 3015
Fragments arranged by size:
4657 3015 216 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4363 5274 1316
Size: 3047 911 4026
Fragments arranged by size:
4026 3047 911
XbaI T'CTAG_A
Cuts at: 3427 5032 5320 3427
Size: 1605 288 6091
Fragments arranged by size:
6091 1605 288
XhoI C'TCGA_G
Cuts at: 1943 2827 1943
Size: 884 7100
XmaI C'CCGG_G
Cuts at: 1870 5309 1870
Size: 3439 4545
XmnI GAAnn'nnTTC
Cuts at: 2152 5208 6113 2152
Size: 3056 905 4023
Fragments arranged by size:
4023 3056 905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5792 276
Size: 53 83 186 936 4258 2468
Fragments arranged by size:
4258 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I DraIII FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI