Restriction map for
pFN21AB3766
(Circular) (Six-base) MAPSORT of: pFN21AB3766.seq Check: 7427 from: 1 to: 6217
pFN21AB3766 6217 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2691 2850 3015 3460 4280 5664 5932 1632
Size: 1059 159 165 445 820 1384 268 1917
Fragments arranged by size:
1917 1384 1059 820 445 268 165 159
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 3262 1166
Size: 2096 4121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 3447 3960 5532 3447
Size: 513 1572 4132
Fragments arranged by size:
4132 1572 513
ApaLI G'TGCA_C
Cuts at: 4274 5430 4274
Size: 1156 5061
ApoI r'AATT_y
Cuts at: 1493 3503 3526 3752 3904 6013 1493
Size: 2010 23 226 152 2109 1697
Fragments arranged by size:
2109 2010 1697 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 3542 1870
Size: 73 1599 4545
Fragments arranged by size:
4545 1599 73
AvrII C'CTAG_G
Cuts at: 2615 2615
Size: 6217
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3267 3538 4993 618
Size: 325 217 627 1480 271 1455 1842
Fragments arranged by size:
1842 1480 1455 627 325 271 217
BanII G_rGCy'C
Cuts at: 1391 2042 2838 3536 1391
Size: 651 796 698 4072
Fragments arranged by size:
4072 796 698 651
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 3121 1548
Size: 1573 4644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2037 4828
Size: 107 122 71 631 605 337 28 2791
Cuts at: 4828 136
Size: 1525
Fragments arranged by size:
2791 1525 631 605 337 122 107 71
28
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 3634 3634
Size: 6217
Bme1580I G_kGCm'C
Cuts at: 1790 4278 5434 1790
Size: 2488 1156 2573
Fragments arranged by size:
2573 2488 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3948 4906 442
Size: 416 53 83 399 24 2531 958 1753
Fragments arranged by size:
2531 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2165 1056
Size: 1109 5108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4877 1239
Size: 3638 2579
Bpu10I CC'TnA_GC
Cuts at: 3102 3481 3102
Size: 379 5838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4342 5207 5505 4342
Size: 865 298 5054
Fragments arranged by size:
5054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2706 4880 882
Size: 617 1207 2174 2219
Fragments arranged by size:
2219 2174 1207 617
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4024 4406 5041 275
Size: 53 83 186 936 2491 382 635 1451
Fragments arranged by size:
2491 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5992
Fragments arranged by size:
5992 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6217
BseYI C'CCAG_C
Cuts at: 2035 5420 5916 2035
Size: 3385 496 2336
Fragments arranged by size:
3385 2336 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2256 1163
Size: 573 520 5124
Fragments arranged by size:
5124 573 520
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2400 2838 4278 4363 5434 2400
Size: 438 1440 85 1071 3183
Fragments arranged by size:
3183 1440 1071 438 85
BsmI GAATG_Cn'
Cuts at: 3031 3728 3821 3031
Size: 697 93 5427
Fragments arranged by size:
5427 697 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2400 2838 3536 4278 4363 5434
Size: 399 252 358 438 698 742 85 1071
Cuts at: 5434 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 698 438 399 358 252
85
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 2886 4106 5836 2886
Size: 1220 1730 3267
Fragments arranged by size:
3267 1730 1220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3572 844
Size: 2728 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4104 1394
Size: 429 2281 3507
Fragments arranged by size:
3507 2281 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2904 4712 4886 65
Size: 1183 1656 1808 174 1396
Fragments arranged by size:
1808 1656 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 4861 5048 1700
Size: 110 221 2830 187 2869
Fragments arranged by size:
2869 2830 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2473 794
Size: 1679 4538
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 2201 1184
Size: 1017 5200
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2035
Size: 6217
BstYI r'GATC_y
Cuts at: 1972 2071 3391 3547 3914 4299 4316 5768 5854
Size: 99 1320 156 367 385 17 1452 86
Cuts at: 5854 5866 6212 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1320 385 367 346 156 99
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4726
Fragments arranged by size:
4726 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2316 2944 3257 3269 3733 4527 4547 2316
Size: 628 313 12 464 794 20 3986
Fragments arranged by size:
3986 794 628 464 313 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 2389 3502 3521 2389
Size: 1113 19 5085
Fragments arranged by size:
5085 1113 19
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3664 4553 6182
Size: 54 1425 50 275 18 1834 889 1629
Cuts at: 6182 8
Size: 43
Fragments arranged by size:
1834 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2826 4147 1353
Size: 1473 1321 3423
Fragments arranged by size:
3423 1473 1321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3501 4804 5178 5324 387
Size: 1064 138 1912 1303 374 146 1280
Fragments arranged by size:
1912 1303 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2836 2836
Size: 6217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2691 2850 3015 3460 4280 4877 5664
Size: 393 1059 159 165 445 820 597 787
Cuts at: 5664 5932 1239
Size: 268 1524
Fragments arranged by size:
1524 1059 820 787 597 445 393 268
165 159
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2291 2329 2381 2291
Size: 38 52 6127
Fragments arranged by size:
6127 52 38
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 5364 1678
Size: 3686 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2159 2191 4858 4890 4932
Size: 32 135 32 587 32 2667 32 42
Cuts at: 4932 4964 1373
Size: 32 2626
Fragments arranged by size:
2667 2626 587 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3561 3807 669
Size: 988 281 1623 246 3079
Fragments arranged by size:
3079 1623 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 2300 3816 2300
Size: 1516 4701
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5331 5515 1305
Size: 4026 184 2007
Fragments arranged by size:
4026 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4395
Fragments arranged by size:
4395 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3262 3513 4175 4534 4693 518
Size: 648 2096 251 662 359 159 2042
Fragments arranged by size:
2096 2042 662 648 359 251 159
MspA1I CmG'CkG
Cuts at: 1606 2153 2828 3335 3633 4310 5458 5703 1606
Size: 547 675 507 298 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 675 547 507 298 245
NaeI GCC'GGC
Cuts at: 2033 2033
Size: 6217
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4726
Fragments arranged by size:
4726 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NgoMIV G'CCGG_C
Cuts at: 2031 2031
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 2161 1052
Size: 1109 5108
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 3575 5120 1132
Size: 97 2346 1545 2229
Fragments arranged by size:
2346 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2712 2744 3302 3334 4286 4318 5799 5831 2712
Size: 32 558 32 952 32 1481 32 3098
Fragments arranged by size:
3098 1481 952 558 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 2450 2600 2682 3192 3787 2450
Size: 150 82 510 595 4880
Fragments arranged by size:
4880 595 510 150 82
PstI C_TGCA'G
Cuts at: 830 2079 2323 3264 3569 830
Size: 1249 244 941 305 3478
Fragments arranged by size:
3478 1249 941 305 244
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2153 2828 3335 2153
Size: 675 507 5035
Fragments arranged by size:
5035 675 507
SacI G_AGCT'C
Cuts at: 2838 2838
Size: 6217
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SapI GCTCTTCn'nnn_
Cuts at: 2826 2826
Size: 6217
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 2075 2319 2523 3260 3565 4700 5381
Size: 219 1030 244 204 737 305 1135 681
Cuts at: 5381 5572 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1030 737 681 305 244 219
204 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4321 5222 5484 820
Size: 197 926 2378 901 262 1553
Fragments arranged by size:
2378 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 3050 1517
Size: 1533 4684
StyI C'CwwG_G
Cuts at: 513 1065 2004 2615 3656 513
Size: 552 939 611 1041 3074
Fragments arranged by size:
3074 1041 939 611 552
SwaI ATTT'AAAT
Cuts at: 3502 3502
Size: 6217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3285 4244 4261 4414 4599 5044 325
Size: 1255 1705 959 17 153 185 445 1498
Fragments arranged by size:
1705 1498 1255 959 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2697 4463 6175
Size: 274 80 33 51 1443 720 1766 1712
Cuts at: 6175 96
Size: 138
Fragments arranged by size:
1766 1712 1443 720 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2708 2740 6177 6209 2708
Size: 32 3437 32 2716
Fragments arranged by size:
3437 2716 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3142 3553 3142
Size: 411 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 3081 3317 4346 3081
Size: 236 1029 4952
Fragments arranged by size:
4952 1029 236
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI XcmI