Restriction map for
pFN21AB3765
(Circular) (Six-base) MAPSORT of: pFN21AB3765.seq Check: 6349 from: 1 to: 6106
pFN21AB3765 6106 bp
With 182 enzymes: SgfI *
April 12, 2013 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2176 2176
Size: 6106
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 2574 4233 4606 1563
Size: 1011 1659 373 3063
Fragments arranged by size:
3063 1659 1011 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4169 5553 5821 1632
Size: 2537 1384 268 1917
Fragments arranged by size:
2537 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 5005 5982 1203
Size: 3802 977 1327
Fragments arranged by size:
3802 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6106
AlwNI CAG_nnn'CTG
Cuts at: 3849 5421 3849
Size: 1572 4534
ApaLI G'TGCA_C
Cuts at: 4163 5319 4163
Size: 1156 4950
ApoI r'AATT_y
Cuts at: 1493 2614 2938 3415 3641 3793 5902 1493
Size: 1121 324 477 226 152 2109 1697
Fragments arranged by size:
2109 1697 1121 477 324 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3248 4660 160
Size: 872 2216 1412 1606
Fragments arranged by size:
2216 1606 1412 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 3431 1870
Size: 73 1488 4545
Fragments arranged by size:
4545 1488 73
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2237 3427 4882 618
Size: 325 217 627 450 1190 1455 1842
Fragments arranged by size:
1842 1455 1190 627 450 325 217
BanII G_rGCy'C
Cuts at: 1391 2392 3425 1391
Size: 1001 1033 4072
Fragments arranged by size:
4072 1033 1001
BbeI G_GCGC'C
Cuts at: 2241 2241
Size: 6106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3126 928
Size: 971 1227 3908
Fragments arranged by size:
3908 1227 971
BbvCI CC'TCA_GC
Cuts at: 3193 3193
Size: 6106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3091 3125 4297 4331 1903
Size: 34 1154 34 1172 34 3678
Fragments arranged by size:
3678 1172 1154 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2941 3116 3998 5208 2941
Size: 175 882 1210 3839
Fragments arranged by size:
3839 1210 882 175
BclI T'GATC_A
Cuts at: 1548 2250 2421 1548
Size: 702 171 5233
Fragments arranged by size:
5233 702 171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2702 4717 136
Size: 107 122 71 631 605 1030 2015 1525
Fragments arranged by size:
2015 1525 1030 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6101 6101
Size: 6106
BlpI GC'TnA_GC
Cuts at: 3523 3523
Size: 6106
Bme1580I G_kGCm'C
Cuts at: 1790 2698 4167 5323 1790
Size: 908 1469 1156 2573
Fragments arranged by size:
2573 1469 1156 908
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3837 4795 442
Size: 416 53 83 399 24 2420 958 1753
Fragments arranged by size:
2420 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2651 4766 1239
Size: 1412 2115 2579
Fragments arranged by size:
2579 2115 1412
Bpu10I CC'TnA_GC
Cuts at: 2069 2145 2829 3193 2069
Size: 76 684 364 4982
Fragments arranged by size:
4982 684 364 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4231 5096 5394 4231
Size: 865 298 4943
Fragments arranged by size:
4943 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2901 4769 882
Size: 617 1402 1868 2219
Fragments arranged by size:
2219 1868 1402 617
BsaAI yAC'GTr
Cuts at: 493 1204 2084 3897 493
Size: 711 880 1813 2702
Fragments arranged by size:
2702 1813 880 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2238 3913 4295 4930
Size: 53 83 186 936 705 1675 382 635
Cuts at: 4930 275
Size: 1451
Fragments arranged by size:
1675 1451 936 705 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4539 4937 5211 5358 5769 4539
Size: 398 274 147 411 4876
Fragments arranged by size:
4876 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2649 2679 1178
Size: 30 165 30 1246 30 4605
Fragments arranged by size:
4605 1246 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2860 1186
Size: 1045 629 4432
Fragments arranged by size:
4432 1045 629
BseYI C'CCAG_C
Cuts at: 5309 5805 5309
Size: 496 5610
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2004 2378 1163
Size: 573 268 374 4891
Fragments arranged by size:
4891 573 374 268
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3556 4317 5345 1540
Size: 275 186 1555 761 1028 2301
Fragments arranged by size:
2301 1555 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 4167 4252 5323 2118
Size: 2049 85 1071 2901
Fragments arranged by size:
2901 2049 1071 85
BsmI GAATG_Cn'
Cuts at: 2047 2413 3386 3617 3710 2047
Size: 366 973 231 93 4443
Fragments arranged by size:
4443 973 366 231 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2392 2698 3425 4167 4252 5323
Size: 399 328 274 306 727 742 85 1071
Cuts at: 5323 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 727 399 328 306 274
85
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 2418 2439 3995 5725 2418
Size: 21 1556 1730 2799
Fragments arranged by size:
2799 1730 1556 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2026 2176 3461 844
Size: 1182 150 1285 3489
Fragments arranged by size:
3489 1285 1182 150
BsrBI CCG'CTC
Cuts at: 1394 1823 3993 1394
Size: 429 2170 3507
Fragments arranged by size:
3507 2170 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3296 4601 4775 65
Size: 1183 2048 1305 174 1396
Fragments arranged by size:
2048 1396 1305 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4750 4937 1700
Size: 110 2940 187 2869
Fragments arranged by size:
2940 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3361 96
Size: 3265 2841
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 3075 4166 5178 3075
Size: 1091 1012 4003
Fragments arranged by size:
4003 1091 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6106
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 3436 3803 4188 4205 5657 5743 5755 6101
Size: 1464 367 385 17 1452 86 12 346
Cuts at: 6101 1972
Size: 1977
Fragments arranged by size:
1977 1464 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3041 487
Size: 683 56 1815 3552
Fragments arranged by size:
3552 1815 683 56
BtsI GCAGTG_nn'
Cuts at: 3622 4416 4436 3622
Size: 794 20 5292
Fragments arranged by size:
5292 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 2563 3410 2563
Size: 847 5259
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 3553 4442
Size: 54 1425 50 275 18 307 1416 889
Cuts at: 4442 6071 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1416 889 307 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3312 4036 1353
Size: 1959 724 3423
Fragments arranged by size:
3423 1959 724
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4693 5067 5213 387
Size: 1064 138 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2651 4169 4766 5553 5821 1239
Size: 393 1019 1518 597 787 268 1524
Fragments arranged by size:
1524 1518 1019 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3050 3050
Size: 6106
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2675 2822 2675
Size: 147 5959
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2241 5253 1678
Size: 563 3012 2531
Fragments arranged by size:
3012 2531 563
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 3025 3057 3069
Size: 32 135 32 566 32 855 32 12
Cuts at: 3069 3101 4747 4779 4821 4853 1373
Size: 32 1646 32 42 32 2626
Fragments arranged by size:
2626 1646 855 566 135 42 32 32
32 32 32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3450 3696 669
Size: 988 281 1512 246 3079
Fragments arranged by size:
3079 1512 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KasI G'GCGC_C
Cuts at: 2237 2237
Size: 6106
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5220 5404 1305
Size: 3915 184 2007
Fragments arranged by size:
3915 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2139 10
Size: 54 1425 343 307 3977
Fragments arranged by size:
3977 1425 343 307 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 4064 4423 4582 518
Size: 648 1119 1779 359 159 2042
Fragments arranged by size:
2042 1779 1119 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3522 4199 5347 5592 1606
Size: 1916 677 1148 245 2120
Fragments arranged by size:
2120 1916 1148 677 245
NarI GG'CG_CC
Cuts at: 2238 2238
Size: 6106
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5554
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2249 3464 5009 1132
Size: 97 1020 1215 1545 2229
Fragments arranged by size:
2229 1545 1215 1020 97
PasI CC'CwG_GG
Cuts at: 1348 2170 1348
Size: 822 5284
PciI A'CATG_T
Cuts at: 5005 5005
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2646 1108
Size: 1538 4568
PfoI T'CCnGG_A
Cuts at: 3087 3087
Size: 6106
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6106
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PstI C_TGCA'G
Cuts at: 830 3458 830
Size: 2628 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SapI GCTCTTCn'nnn_
Cuts at: 3312 3312
Size: 6106
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SfcI C'TryA_G
Cuts at: 826 1045 3169 3454 4589 5270 5461 826
Size: 219 2124 285 1135 681 191 1471
Fragments arranged by size:
2124 1471 1135 681 285 219 191
SfoI GGC'GCC
Cuts at: 2239 2239
Size: 6106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3433 1872
Size: 1561 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4210 5111 5373 820
Size: 197 926 2267 901 262 1553
Fragments arranged by size:
2267 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2084 3897 493
Size: 1591 1813 2702
Fragments arranged by size:
2702 1813 1591
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 3464 1132
Size: 2332 3774
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 2134 3545 513
Size: 552 1069 1411 3074
Fragments arranged by size:
3074 1411 1069 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4133 4150 4303 4488 4933 325
Size: 1255 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3361 4352 6064
Size: 274 80 33 51 1443 1384 991 1712
Cuts at: 6064 96
Size: 138
Fragments arranged by size:
1712 1443 1384 991 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2605 2637 2649 2681 6066 6098 2605
Size: 32 12 32 3385 32 2613
Fragments arranged by size:
3385 2613 32 32 32 12
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 3431 1870
Size: 1561 4545
XmnI GAAnn'nnTTC
Cuts at: 2635 4235 2635
Size: 1600 4506
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI XcmI