Restriction map for
pFN21AB3758
(Circular) (Six-base) MAPSORT of: pFN21AB3758.seq Check: 6423 from: 1 to: 6487
pFN21AB3758 6487 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3358 3358
Size: 6487
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2526 4297 278
Size: 53 83 186 936 990 1771 2468
Fragments arranged by size:
2468 1771 990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3830 1937
Size: 1893 4594
Acc65I G'GTAC_C
Cuts at: 3808 3808
Size: 6487
AclI AA'CG_TT
Cuts at: 1563 4614 4987 1563
Size: 3051 373 3063
Fragments arranged by size:
3063 3051 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3200 4550 5934 6202 1632
Size: 1568 1350 1384 268 1917
Fragments arranged by size:
1917 1568 1384 1350 268
AfeI AGC'GCT
Cuts at: 3194 3194
Size: 6487
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6290
AflIII A'CryG_T
Cuts at: 1203 5386 6363 1203
Size: 4183 977 1327
Fragments arranged by size:
4183 1327 977
AgeI A'CCGG_T
Cuts at: 5318 5318
Size: 6487
AhdI GACnn_n'nnGTC
Cuts at: 5216 5216
Size: 6487
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6487
AlwNI CAG_nnn'CTG
Cuts at: 2644 2780 3178 3278 3664 4230 5802 2644
Size: 136 398 100 386 566 1572 3329
Fragments arranged by size:
3329 1572 566 398 386 136 100
ApaI G_GGCC'C
Cuts at: 3629 3745 3629
Size: 116 6371
ApaLI G'TGCA_C
Cuts at: 2079 4544 5700 2079
Size: 2465 1156 2866
Fragments arranged by size:
2866 2465 1156
ApoI r'AATT_y
Cuts at: 1493 3796 4022 4174 6283 1493
Size: 2303 226 152 2109 1697
Fragments arranged by size:
2303 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5041 160
Size: 872 4009 1606
Fragments arranged by size:
4009 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 2054 2160 2349 2423 3812 1870
Size: 73 68 43 106 189 74 1389 4545
Fragments arranged by size:
4545 1389 189 106 74 73 68 43
BamHI G'GATC_C
Cuts at: 2156 3817 4184 2156
Size: 1661 367 4459
Fragments arranged by size:
4459 1661 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2247 3454 3487 3808 5263
Size: 325 217 627 460 1207 33 321 1455
Cuts at: 5263 618
Size: 1842
Fragments arranged by size:
1842 1455 1207 627 460 325 321 217
33
BanII G_rGCy'C
Cuts at: 1391 2115 2209 2245 2705 2760 3246 3629 3745
Size: 724 94 36 460 55 486 383 116
Cuts at: 3745 3806 1391
Size: 61 4072
Fragments arranged by size:
4072 724 486 460 383 116 94 61
55 36
BbeI G_GCGC'C
Cuts at: 2251 2251
Size: 6487
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2399 2526 2868 928
Size: 971 500 127 342 4547
Fragments arranged by size:
4547 971 500 342 127
BbvCI CC'TCA_GC
Cuts at: 2092 2966 3237 3387 3495 3701 2092
Size: 874 271 150 108 206 4878
Fragments arranged by size:
4878 874 271 206 150 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4678 4712 1903
Size: 34 50 34 2657 34 3678
Fragments arranged by size:
3678 2657 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4379 5589 4379
Size: 1210 5277
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6487
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2222 3611 5098
Size: 107 122 71 631 605 550 1389 1487
Cuts at: 5098 136
Size: 1525
Fragments arranged by size:
1525 1487 1389 631 605 550 122 107
71
BglII A'GATC_T
Cuts at: 6482 6482
Size: 6487
BlpI GC'TnA_GC
Cuts at: 2704 3096 3904 2704
Size: 392 808 5287
Fragments arranged by size:
5287 808 392
Bme1580I G_kGCm'C
Cuts at: 1790 2083 2141 3459 3629 3734 3745 4548 5704
Size: 293 58 1318 170 105 11 803 1156
Cuts at: 5704 1790
Size: 2573
Fragments arranged by size:
2573 1318 1156 803 293 170 105 58
11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6487
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2173 4218 5176
Size: 416 53 83 399 24 756 2045 958
Cuts at: 5176 442
Size: 1753
Fragments arranged by size:
2045 1753 958 756 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2247 2486 2567 2747 2996 3074 3584 3629
Size: 1008 239 81 180 249 78 510 45
Cuts at: 3629 3641 3701 5147 1239
Size: 12 60 1446 2579
Fragments arranged by size:
2579 1446 1008 510 249 239 180 81
78 60 45 12
Bpu10I CC'TnA_GC
Cuts at: 2092 2439 2895 2966 3119 3237 3387 3495 3701
Size: 347 456 71 153 118 150 108 206
Cuts at: 3701 2092
Size: 4878
Fragments arranged by size:
4878 456 347 206 153 150 118 108
71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3047 4612 5477 5775 3047
Size: 1565 865 298 3759
Fragments arranged by size:
3759 1565 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3530 5150 882
Size: 617 2031 1620 2219
Fragments arranged by size:
2219 2031 1620 617
BsaAI yAC'GTr
Cuts at: 493 1204 4278 493
Size: 711 3074 2702
Fragments arranged by size:
3074 2702 711
BsaBI GATnn'nnATC
Cuts at: 6481 6481
Size: 6487
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2248 2396 2523 4294
Size: 53 83 186 936 715 148 127 1771
Cuts at: 4294 4676 5311 275
Size: 382 635 1451
Fragments arranged by size:
1771 1451 936 715 635 382 186 148
127 83 53
BsaWI w'CCGG_w
Cuts at: 2107 2201 2283 4920 5318 5592 5739 6150 2107
Size: 94 82 2637 398 274 147 411 2444
Fragments arranged by size:
2637 2444 411 398 274 147 94 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6262
Fragments arranged by size:
6262 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 2564 2687 2729 2750 2801 2822 2825
Size: 843 535 123 42 21 51 21 3
Cuts at: 2825 2993 2996 3254 3461 3479 3548 3566 1186
Size: 168 3 258 207 18 69 18 4107
Fragments arranged by size:
4107 843 535 258 207 168 123 69
51 42 21 21 18 18 3 3
BseYI C'CCAG_C
Cuts at: 2062 2698 3469 3546 5690 6186 2062
Size: 636 771 77 2144 496 2363
Fragments arranged by size:
2363 2144 771 636 496 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2906 1163
Size: 573 1170 4744
Fragments arranged by size:
4744 1170 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3937 4698 5726 1540
Size: 275 186 1936 761 1028 2301
Fragments arranged by size:
2301 1936 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2083 2209 2760 2878 3120 3246 4548 4633 5704
Size: 126 551 118 242 126 1302 85 1071
Cuts at: 5704 2083
Size: 2866
Fragments arranged by size:
2866 1302 1071 551 242 126 126 118
85
BsmI GAATG_Cn'
Cuts at: 2258 3998 4091 2258
Size: 1740 93 4654
Fragments arranged by size:
4654 1740 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3221 3258 3221
Size: 37 6450
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2115 2141 2209 2245 2705 2760
Size: 399 293 32 26 68 36 460 55
Cuts at: 2760 2878 3120 3246 3459 3629 3734 3745 3806
Size: 118 242 126 213 170 105 11 61
Cuts at: 3806 4548 4633 5704 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 460 399 293 242 213
170 126 118 105 85 68 61 55
36 32 26 11
BspEI T'CCGG_A
Cuts at: 2107 6150 2107
Size: 4043 2444
BspHI T'CATG_A
Cuts at: 4376 6106 4376
Size: 1730 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2874 3358 3842 844
Size: 2030 484 484 3489
Fragments arranged by size:
3489 2030 484 484
BsrBI CCG'CTC
Cuts at: 1394 1823 2105 4374 1394
Size: 429 282 2269 3507
Fragments arranged by size:
3507 2269 429 282
BsrDI GCAATG_nn'
Cuts at: 65 1248 2915 4982 5156 65
Size: 1183 1667 2067 174 1396
Fragments arranged by size:
2067 1667 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2025 2043 2415 3490 5131 5318 1700
Size: 110 215 18 372 1075 1641 187 2869
Fragments arranged by size:
2869 1641 1075 372 215 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6487
BssHII G'CGCG_C
Cuts at: 1925 2443 1925
Size: 518 5969
BssSI C'ACGA_G
Cuts at: 4547 5559 4547
Size: 1012 5475
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6487
BstBI TT'CG_AA
Cuts at: 5291 5291
Size: 6487
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6487
BstXI CCAn_nnnn'nTGG
Cuts at: 2568 3622 2568
Size: 1054 5433
BstYI r'GATC_y
Cuts at: 1972 2156 3817 4184 4569 4586 6038 6124 6136
Size: 184 1661 367 385 17 1452 86 12
Cuts at: 6136 6482 1972
Size: 346 1977
Fragments arranged by size:
1977 1661 1452 385 367 346 184 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3332 3332
Size: 6487
BtgI C'CryG_G
Cuts at: 513 1065 2312 3736 513
Size: 552 1247 1424 3264
Fragments arranged by size:
3264 1424 1247 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5748
Fragments arranged by size:
5748 683 56
BtsI GCAGTG_nn'
Cuts at: 2619 2876 2932 4003 4797 4817 2619
Size: 257 56 1071 794 20 4289
Fragments arranged by size:
4289 1071 794 257 56 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6487
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6452
DraI TTT'AAA
Cuts at: 3791 3791
Size: 6487
DraIII CAC_nnn'GTG
Cuts at: 2740 2740
Size: 6487
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5494 809
Size: 507 4178 1802
Fragments arranged by size:
4178 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2265 2359 2413
Size: 54 1425 50 275 18 435 94 54
Cuts at: 2413 2635 3666 3934 4823 6452 8
Size: 222 1031 268 889 1629 43
Fragments arranged by size:
1629 1425 1031 889 435 275 268 222
94 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3934 1537
Size: 275 2122 4090
Fragments arranged by size:
4090 2122 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2799 2826 2997 3216 3507 4417 1353
Size: 1446 27 171 219 291 910 3423
Fragments arranged by size:
3423 1446 910 291 219 171 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2484 5074 5448 5594 387
Size: 1064 138 895 2590 374 146 1280
Fragments arranged by size:
2590 1280 1064 895 374 146 138
EcoICRI GAG'CTC
Cuts at: 2207 2758 3244 2207
Size: 551 486 5450
Fragments arranged by size:
5450 551 486
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2247 2486 2567 2747 2996 3074 3200
Size: 393 615 239 81 180 249 78 126
Cuts at: 3200 3584 3629 3641 3701 4550 5147 5934 6202
Size: 384 45 12 60 849 597 787 268
Cuts at: 6202 1239
Size: 1524
Fragments arranged by size:
1524 849 787 615 597 393 384 268
249 239 180 126 81 78 60 45
12
EcoO109I rG'GnC_Cy
Cuts at: 2059 2611 3626 3741 3742 2059
Size: 552 1015 115 1 4804
Fragments arranged by size:
4804 1015 552 115 1
EcoRI G'AATT_C
Cuts at: 3796 4174 3796
Size: 378 6109
EcoRV GAT'ATC
Cuts at: 3415 3415
Size: 6487
FspI TGC'GCA
Cuts at: 2121 4993 2121
Size: 2872 3615
HaeII r_GCGC'y
Cuts at: 1678 2251 3196 5634 1678
Size: 573 945 2438 2531
Fragments arranged by size:
2531 2438 945 573
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 3193 3225 3512
Size: 32 135 32 698 32 891 32 287
Cuts at: 3512 3544 5128 5160 5202 5234 1373
Size: 32 1584 32 42 32 2626
Fragments arranged by size:
2626 1584 891 698 287 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3831 4077 669
Size: 988 281 1893 246 3079
Fragments arranged by size:
3079 1893 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6487
HpaI GTT'AAC
Cuts at: 4077 4077
Size: 6487
KasI G'GCGC_C
Cuts at: 2247 2247
Size: 6487
KpnI G_GTAC'C
Cuts at: 3812 3812
Size: 6487
MfeI C'AATT_G
Cuts at: 4086 4086
Size: 6487
MluI A'CGCG_T
Cuts at: 6363 6363
Size: 6487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3084 5601 5785 1305
Size: 1779 2517 184 2007
Fragments arranged by size:
2517 2007 1779 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3668 10
Size: 54 1425 343 1836 2829
Fragments arranged by size:
2829 1836 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2883 4445 4804 4963 518
Size: 648 1717 1562 359 159 2042
Fragments arranged by size:
2042 1717 1562 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2381 2944 2995 3410 3618 3664 3700 3903
Size: 775 563 51 415 208 46 36 203
Cuts at: 3903 4580 5728 5973 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 775 677 563 415 245 208
203 51 46 36
NaeI GCC'GGC
Cuts at: 2045 2417 3492 2045
Size: 372 1075 5040
Fragments arranged by size:
5040 1075 372
NarI GG'CG_CC
Cuts at: 2248 2248
Size: 6487
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5935
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6487
NgoMIV G'CCGG_C
Cuts at: 2043 2415 3490 2043
Size: 372 1075 5040
Fragments arranged by size:
5040 1075 372
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6487
NotI GC'GGCC_GC
Cuts at: 3934 3934
Size: 6487
NspI r_CATG'y
Cuts at: 1132 1229 3845 5390 1132
Size: 97 2616 1545 2229
Fragments arranged by size:
2616 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6487
PciI A'CATG_T
Cuts at: 5386 5386
Size: 6487
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 3091 3664 1108
Size: 1536 447 573 3931
Fragments arranged by size:
3931 1536 573 447
PfoI T'CCnGG_A
Cuts at: 3222 3222
Size: 6487
PmeI GTTT'AAAC
Cuts at: 3791 3791
Size: 6487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3030 3062 4556 4588 6069 6101 3030
Size: 32 1494 32 1481 32 3416
Fragments arranged by size:
3416 1494 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2611 2059
Size: 552 5935
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6487
PsiI TTA'TAA
Cuts at: 4057 4057
Size: 6487
PspOMI G'GGCC_C
Cuts at: 3625 3741 3625
Size: 116 6371
PstI C_TGCA'G
Cuts at: 830 2601 2626 2871 3168 3189 3773 3839 830
Size: 1771 25 245 297 21 584 66 3478
Fragments arranged by size:
3478 1771 584 297 245 66 25 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6487
PvuII CAG'CTG
Cuts at: 2944 2995 3664 3700 2944
Size: 51 669 36 5731
Fragments arranged by size:
5731 669 51 36
SacI G_AGCT'C
Cuts at: 2209 2760 3246 2209
Size: 551 486 5450
Fragments arranged by size:
5450 551 486
SalI G'TCGA_C
Cuts at: 1936 3829 1936
Size: 1893 4594
SanDI GG'GwC_CC
Cuts at: 2059 2059
Size: 6487
SbfI CC_TGCA'GG
Cuts at: 3839 3839
Size: 6487
ScaI AGT'ACT
Cuts at: 4735 6447 4735
Size: 1712 4775
SexAI A'CCwGG_T
Cuts at: 2736 2736
Size: 6487
SfcI C'TryA_G
Cuts at: 826 1045 2597 2622 2867 3164 3185 3349 3769
Size: 219 1552 25 245 297 21 164 420
Cuts at: 3769 3835 4970 5651 5842 826
Size: 66 1135 681 191 1471
Fragments arranged by size:
1552 1471 1135 681 420 297 245 219
191 164 66 25 21
SfoI GGC'GCC
Cuts at: 2249 2249
Size: 6487
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6487
SmaI CCC'GGG
Cuts at: 1872 2056 2351 2425 3814 1872
Size: 184 295 74 1389 4545
Fragments arranged by size:
4545 1389 295 184 74
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3026 4591 5492 5754 820
Size: 197 926 1083 1565 901 262 1553
Fragments arranged by size:
1565 1553 1083 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4278 493
Size: 3785 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6487
SphI G_CATG'C
Cuts at: 1132 3845 1132
Size: 2713 3774
SspI AAT'ATT
Cuts at: 5 52 4411 5
Size: 47 4359 2081
Fragments arranged by size:
4359 2081 47
StuI AGG'CCT
Cuts at: 1517 3008 1517
Size: 1491 4996
StyI C'CwwG_G
Cuts at: 513 1065 2232 2272 2332 2709 3926 513
Size: 552 1167 40 60 377 1217 3074
Fragments arranged by size:
3074 1217 1167 552 377 60 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2528 3554 4514 4531 4684 4869 5314
Size: 1255 948 1026 960 17 153 185 445
Cuts at: 5314 325
Size: 1498
Fragments arranged by size:
1498 1255 1026 960 948 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4733 6445 96
Size: 274 80 33 51 1443 2756 1712 138
Fragments arranged by size:
2756 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2297 2329 2462 2494 6447 6479 2297
Size: 32 133 32 3953 32 2305
Fragments arranged by size:
3953 2305 133 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6487
XbaI T'CTAG_A
Cuts at: 3823 3823
Size: 6487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2339 3088 2339
Size: 749 5738
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6487
XmaI C'CCGG_G
Cuts at: 1870 2054 2349 2423 3812 1870
Size: 184 295 74 1389 4545
Fragments arranged by size:
4545 1389 295 184 74
XmnI GAAnn'nnTTC
Cuts at: 4616 4616
Size: 6487
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2524 4295 276
Size: 53 83 186 936 990 1771 2468
Fragments arranged by size:
2468 1771 990 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
EcoNI FalI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI