Restriction map for
pFN21AB3737
(Circular) (Six-base) MAPSORT of: pFN21AB3737.seq Check: 3425 from: 1 to: 5956
pFN21AB3737 5956 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2160 2770 3227 2160
Size: 610 457 4889
Fragments arranged by size:
4889 610 457
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3166 4019 5403 5671 1632
Size: 1534 853 1384 268 1917
Fragments arranged by size:
1917 1534 1384 853 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AlwNI CAG_nnn'CTG
Cuts at: 3699 5271 3699
Size: 1572 4384
ApaI G_GGCC'C
Cuts at: 2024 3035 2024
Size: 1011 4945
ApaLI G'TGCA_C
Cuts at: 2147 2538 4013 5169 2147
Size: 391 1475 1156 2934
Fragments arranged by size:
2934 1475 1156 391
ApoI r'AATT_y
Cuts at: 1493 3265 3491 3643 5752 1493
Size: 1772 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2826 3084 3277 4732 618
Size: 325 217 627 1039 258 193 1455 1842
Fragments arranged by size:
1842 1455 1039 627 325 258 217 193
BanII G_rGCy'C
Cuts at: 1391 2024 3035 3231 3275 1391
Size: 633 1011 196 44 4072
Fragments arranged by size:
4072 1011 633 196 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4985
BbvCI CC'TCA_GC
Cuts at: 2119 2119
Size: 5956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2545 3848 5058 2545
Size: 1303 1210 3443
Fragments arranged by size:
3443 1303 1210
BclI T'GATC_A
Cuts at: 1548 3042 1548
Size: 1494 4462
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2453 4567 136
Size: 107 122 71 631 605 781 2114 1525
Fragments arranged by size:
2114 1525 781 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2876 5951 2876
Size: 3075 2881
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2151 2270 2464 2542 2725 2829 3035
Size: 234 127 119 194 78 183 104 206
Cuts at: 3035 3109 4017 5173 1790
Size: 74 908 1156 2573
Fragments arranged by size:
2573 1156 908 234 206 194 183 127
119 104 78 74
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2027 2428 3208
Size: 416 53 83 399 24 610 401 780
Cuts at: 3208 3687 4645 442
Size: 479 958 1753
Fragments arranged by size:
1753 958 780 610 479 416 401 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2701 2816 2962 4616 1239
Size: 1462 115 146 1654 2579
Fragments arranged by size:
2579 1654 1462 146 115
Bpu10I CC'TnA_GC
Cuts at: 2119 2969 2119
Size: 850 5106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2347 4619 882
Size: 617 848 2272 2219
Fragments arranged by size:
2272 2219 848 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3763 4145 4780 275
Size: 53 83 186 936 2230 382 635 1451
Fragments arranged by size:
2230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2871 4389 4787 5061 5208 5619 2871
Size: 1518 398 274 147 411 3208
Fragments arranged by size:
3208 1518 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2381 2411 1178
Size: 30 165 30 978 30 4723
Fragments arranged by size:
4723 978 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 2813 3002 3005 1186
Size: 1219 408 189 3 4137
Fragments arranged by size:
4137 1219 408 189 3
BseYI C'CCAG_C
Cuts at: 2023 2035 2371 2411 2611 2732 3204 5159 5655
Size: 12 336 40 200 121 472 1955 496
Cuts at: 5655 2023
Size: 2324
Fragments arranged by size:
2324 1955 496 472 336 200 121 40
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2930 2949 1163
Size: 573 1194 19 4170
Fragments arranged by size:
4170 1194 573 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2659 3406 4167 5195 1540
Size: 275 186 658 747 761 1028 2301
Fragments arranged by size:
2301 1028 761 747 658 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 2542 2958 4017 4102 5173 2151
Size: 391 416 1059 85 1071 2934
Fragments arranged by size:
2934 1071 1059 416 391 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2151 2270 2464 2542 2725 2829
Size: 399 234 127 119 194 78 183 104
Cuts at: 2829 2958 3035 3109 3231 3275 4017 4102 5173
Size: 129 77 74 122 44 742 85 1071
Cuts at: 5173 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 234 194 183 129
127 122 119 104 85 78 77 74
44
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 2458 2770 3227 3311 844
Size: 1316 298 312 457 84 3489
Fragments arranged by size:
3489 1316 457 312 298 84
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2629 4451 4625 65
Size: 1183 1381 1822 174 1396
Fragments arranged by size:
1822 1396 1381 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2654 2945 4600 4787 1700
Size: 110 844 291 1655 187 2869
Fragments arranged by size:
2869 1655 844 291 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2327 794
Size: 1533 4423
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2017
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 2337 2876 3115 3286 3653 4038 4055 5507
Size: 365 539 239 171 367 385 17 1452
Cuts at: 5507 5593 5605 5951 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 539 385 367 365 346 239
171 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2490 2490
Size: 5956
BtgI C'CryG_G
Cuts at: 513 1065 2185 2584 3088 3094 513
Size: 552 1120 399 504 6 3375
Fragments arranged by size:
3375 1120 552 504 399 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2774 3077 487
Size: 683 56 1548 303 3366
Fragments arranged by size:
3366 1548 683 303 56
BtsI GCAGTG_nn'
Cuts at: 2382 3472 4266 4286 2382
Size: 1090 794 20 4052
Fragments arranged by size:
4052 1090 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DraIII CAC_nnn'GTG
Cuts at: 2129 2129
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2188 2656
Size: 54 1425 50 275 18 232 126 468
Cuts at: 2656 3091 3149 3403 4292 5921 8
Size: 435 58 254 889 1629 43
Fragments arranged by size:
1629 1425 889 468 435 275 254 232
126 58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2656 3403 1537
Size: 275 844 747 4090
Fragments arranged by size:
4090 844 747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2732 3886 1353
Size: 1379 1154 3423
Fragments arranged by size:
3423 1379 1154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2849 4543 4917 5063 387
Size: 1064 138 1260 1694 374 146 1280
Fragments arranged by size:
1694 1280 1260 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2701 2816 2962 3166 4019 4616 5403
Size: 393 1069 115 146 204 853 597 787
Cuts at: 5403 5671 1239
Size: 268 1524
Fragments arranged by size:
1524 1069 853 787 597 393 268 204
146 115
EcoNI CCTnn'n_nnAGG
Cuts at: 2075 2798 2894 2075
Size: 723 96 5137
Fragments arranged by size:
5137 723 96
EcoO109I rG'GnC_Cy
Cuts at: 2080 2965 3032 2080
Size: 885 67 5004
Fragments arranged by size:
5004 885 67
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 3103 5103 1678
Size: 1425 2000 2531
Fragments arranged by size:
2531 2000 1425
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3161 3193 4597 4629 4671
Size: 32 135 32 1589 32 1404 32 42
Cuts at: 4671 4703 1373
Size: 32 2626
Fragments arranged by size:
2626 1589 1404 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2670 2809 5070 5254 1305
Size: 1365 139 2261 184 2007
Fragments arranged by size:
2261 2007 1365 184 139
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2064 2190 3093 3151 10
Size: 54 1425 343 232 126 903 58 2815
Fragments arranged by size:
2815 1425 903 343 232 126 58 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2921 3006 3914 4273 4432 518
Size: 648 1755 85 908 359 159 2042
Fragments arranged by size:
2042 1755 908 648 359 159 85
MspA1I CmG'CkG
Cuts at: 1606 2661 3082 3186 3372 4049 5197 5442 1606
Size: 1055 421 104 186 677 1148 245 2120
Fragments arranged by size:
2120 1148 1055 677 421 245 186 104
NaeI GCC'GGC
Cuts at: 2947 2947
Size: 5956
NcoI C'CATG_G
Cuts at: 513 1065 2584 3088 513
Size: 552 1519 504 3381
Fragments arranged by size:
3381 1519 552 504
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 2945 2945
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3314 4859 1132
Size: 97 2085 1545 2229
Fragments arranged by size:
2229 2085 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2262 1348
Size: 914 5042
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2618 1108
Size: 1510 4446
PfoI T'CCnGG_A
Cuts at: 3118 3118
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2965 2080
Size: 885 5071
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PspOMI G'GGCC_C
Cuts at: 2020 3031 2020
Size: 1011 4945
PstI C_TGCA'G
Cuts at: 830 3074 3308 830
Size: 2244 234 3478
Fragments arranged by size:
3478 2244 234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 3186 3186
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SexAI A'CCwGG_T
Cuts at: 3015 3015
Size: 5956
SfcI C'TryA_G
Cuts at: 826 1045 3070 3304 4439 5120 5311 826
Size: 219 2025 234 1135 681 191 1471
Fragments arranged by size:
2025 1471 1135 681 234 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4060 4961 5223 820
Size: 197 926 2117 901 262 1553
Fragments arranged by size:
2117 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 2510 2777 2804 3235 1517
Size: 993 267 27 431 4238
Fragments arranged by size:
4238 993 431 267 27
StyI C'CwwG_G
Cuts at: 513 1065 2584 3027 3088 3163 3395 513
Size: 552 1519 443 61 75 232 3074
Fragments arranged by size:
3074 1519 552 443 232 75 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2764 3983 4000 4153 4338 4783 325
Size: 1255 1184 1219 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1219 1184 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2381 2413 5916 5948 2381
Size: 32 3503 32 2389
Fragments arranged by size:
3503 2389 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3061 3086 3061
Size: 25 5931
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 3135 3179 4085 3135
Size: 44 906 5006
Fragments arranged by size:
5006 906 44
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI