Restriction map for
pFN21AB3734
(Circular) (Six-base) MAPSORT of: pFN21AB3734.seq Check: 4574 from: 1 to: 6088
pFN21AB3734 6088 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 2168 3299 4151 5535 5803 1632
Size: 495 41 1131 852 1384 268 1917
Fragments arranged by size:
1917 1384 1131 852 495 268 41
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 3831 5403 3831
Size: 1572 4516
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 3397 3623 3775 5884 1493
Size: 1904 226 152 2109 1697
Fragments arranged by size:
2109 1904 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 3234 3413 1870
Size: 73 1291 179 4545
Fragments arranged by size:
4545 1291 179 73
BamHI G'GATC_C
Cuts at: 2101 3418 3785 2101
Size: 1317 367 4404
Fragments arranged by size:
4404 1317 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3409 4864 618
Size: 325 217 627 1622 1455 1842
Fragments arranged by size:
1842 1622 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2412 3258 3407 1391
Size: 1021 846 149 4072
Fragments arranged by size:
4072 1021 846 149
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4279 4313 1903
Size: 34 2342 34 3678
Fragments arranged by size:
3678 2342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4699 136
Size: 107 122 71 631 605 3027 1525
Fragments arranged by size:
3027 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2346 6083 2346
Size: 3737 2351
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 4149 5305 1790
Size: 2359 1156 2573
Fragments arranged by size:
2573 2359 1156
BmgBI CAC'GTC
Cuts at: 1318 2801 1318
Size: 1483 4605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2212 2474 3819
Size: 416 53 83 399 24 795 262 1345
Cuts at: 3819 4777 442
Size: 958 1753
Fragments arranged by size:
1753 1345 958 795 416 399 262 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 2091 4748 1239
Size: 829 23 2657 2579
Fragments arranged by size:
2657 2579 829 23
Bpu10I CC'TnA_GC
Cuts at: 2130 2130
Size: 6088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4213 5078 5376 4213
Size: 865 298 4925
Fragments arranged by size:
4925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3879 493
Size: 711 2675 2702
Fragments arranged by size:
2702 2675 711
BsaBI GATnn'nnATC
Cuts at: 3093 6082 3093
Size: 2989 3099
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4521 4919 5193 5340 5751 4521
Size: 398 274 147 411 4858
Fragments arranged by size:
4858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3314 3344 1178
Size: 30 165 30 1911 30 3922
Fragments arranged by size:
3922 1911 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 1186
Size: 1051 5037
BseYI C'CCAG_C
Cuts at: 2992 5291 5787 2992
Size: 2299 496 3293
Fragments arranged by size:
3293 2299 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5515
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2412 3294 4149 4234 5305 2412
Size: 882 855 85 1071 3195
Fragments arranged by size:
3195 1071 882 855 85
BsmI GAATG_Cn'
Cuts at: 3140 3599 3692 3140
Size: 459 93 5536
Fragments arranged by size:
5536 459 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2412 3258 3294 3407 4149 4234 5305
Size: 399 622 846 36 113 742 85 1071
Cuts at: 5305 1391
Size: 2174
Fragments arranged by size:
2174 1071 846 742 622 399 113 85
36
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 2815 3977 5707 2815
Size: 1162 1730 3196
Fragments arranged by size:
3196 1730 1162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3060 3443 844
Size: 2216 383 3489
Fragments arranged by size:
3489 2216 383
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4732 4919 1700
Size: 110 2922 187 2869
Fragments arranged by size:
2922 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 3267 96
Size: 1911 1260 2917
Fragments arranged by size:
2917 1911 1260
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 4148 5160 4148
Size: 1012 5076
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6088
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2101 2346 2925 3418 3785 4170 4187 5639
Size: 129 245 579 493 367 385 17 1452
Cuts at: 5639 5725 5737 6083 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 579 493 385 367 346 245
129 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2400 513
Size: 552 1335 4201
Fragments arranged by size:
4201 1335 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2049 487
Size: 683 56 823 4526
Fragments arranged by size:
4526 823 683 56
BtsI GCAGTG_nn'
Cuts at: 2696 3228 3604 4398 4418 2696
Size: 532 376 794 20 4366
Fragments arranged by size:
4366 794 532 376 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DraIII CAC_nnn'GTG
Cuts at: 3217 3217
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3192 3535 4424
Size: 54 1425 50 275 18 1362 343 889
Cuts at: 4424 6053 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1362 889 343 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2187 2570 2633 2679 4018 1353
Size: 765 69 383 63 46 1339 3423
Fragments arranged by size:
3423 1339 765 383 69 63 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2410 2410
Size: 6088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2091 2127 2168 3299 4151 4748
Size: 393 436 23 36 41 1131 852 597
Cuts at: 4748 5535 5803 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1131 852 787 597 436 393 268
41 36 23
EcoO109I rG'GnC_Cy
Cuts at: 2259 2259
Size: 6088
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
EcoRV GAT'ATC
Cuts at: 3091 3091
Size: 6088
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 5235 1678
Size: 3557 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2917 2949 4729 4761 4803
Size: 32 135 32 1345 32 1780 32 42
Cuts at: 4803 4835 1373
Size: 32 2626
Fragments arranged by size:
2626 1780 1345 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2655 2856 748
Size: 1907 201 3980
Fragments arranged by size:
3980 1907 201
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4266
Fragments arranged by size:
4266 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2358 3063 4046 4405 4564 518
Size: 648 1192 705 983 359 159 2042
Fragments arranged by size:
2042 1192 983 705 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2917 3504 4181 5329 5574 1606
Size: 1311 587 677 1148 245 2120
Fragments arranged by size:
2120 1311 1148 677 587 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5536
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 3446 4991 1132
Size: 97 2217 1545 2229
Fragments arranged by size:
2229 2217 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6088
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2259 2259
Size: 6088
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PstI C_TGCA'G
Cuts at: 830 3440 830
Size: 2610 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 2917 2917
Size: 6088
SacI G_AGCT'C
Cuts at: 2412 2412
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SapI GCTCTTCn'nnn_
Cuts at: 2118 2679 2118
Size: 561 5527
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SexAI A'CCwGG_T
Cuts at: 3074 3074
Size: 6088
SfcI C'TryA_G
Cuts at: 826 1045 3223 3436 4571 5252 5443 826
Size: 219 2178 213 1135 681 191 1471
Fragments arranged by size:
2178 1471 1135 681 219 213 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4192 5093 5355 820
Size: 197 926 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 2524 2679 4012 5
Size: 47 2472 155 1333 2081
Fragments arranged by size:
2472 2081 1333 155 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 3312 3527 513
Size: 552 2247 215 3074
Fragments arranged by size:
3074 2247 552 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 4115 4132 4285 4470 4915 325
Size: 1255 1215 1320 17 153 185 445 1498
Fragments arranged by size:
1498 1320 1255 1215 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 2445 3100
Size: 274 80 33 51 1443 30 438 655
Cuts at: 3100 3267 4334 6046 96
Size: 167 1067 1712 138
Fragments arranged by size:
1712 1443 1067 655 438 274 167 138
80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3314 3346 6048 6080 3314
Size: 32 2702 32 3322
Fragments arranged by size:
3322 2702 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 2833 3424 2833
Size: 591 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI