Restriction map for
pFN21AB3732
(Circular) (Six-base) MAPSORT of: pFN21AB3732.seq Check: 6883 from: 1 to: 6088
pFN21AB3732 6088 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2794 3107 3129 4151 5535 5803 1632
Size: 492 670 313 22 1022 1384 268 1917
Fragments arranged by size:
1917 1384 1022 670 492 313 268 22
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 2372 4987 5964 1203
Size: 1169 2615 977 1327
Fragments arranged by size:
2615 1327 1169 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 2718 3831 5403 2718
Size: 1113 1572 3403
Fragments arranged by size:
3403 1572 1113
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 2735 3397 3623 3775 5884 1493
Size: 1242 662 226 152 2109 1697
Fragments arranged by size:
2109 1697 1242 662 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 3154 3413 1870
Size: 73 1211 259 4545
Fragments arranged by size:
4545 1211 259 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2350 2383 2350
Size: 33 6055
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3409 4864 618
Size: 325 217 627 1622 1455 1842
Fragments arranged by size:
1842 1622 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2322 2646 2667 3407 1391
Size: 931 324 21 740 4072
Fragments arranged by size:
4072 931 740 324 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2754 2907 3078 3135 928
Size: 971 855 153 171 57 3881
Fragments arranged by size:
3881 971 855 171 153 57
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2466 2500 4279 4313 1903
Size: 34 529 34 1779 34 3678
Fragments arranged by size:
3678 1779 529 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BfrBI ATG'CAT
Cuts at: 2511 2511
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2693 4699 136
Size: 107 122 71 631 605 1021 2006 1525
Fragments arranged by size:
2006 1525 1021 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3283 6083 3283
Size: 2800 3288
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 2254 2634 4149 5305 1790
Size: 464 380 1515 1156 2573
Fragments arranged by size:
2573 1515 1156 464 380
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2292 3819 4777
Size: 416 53 83 399 24 875 1527 958
Cuts at: 4777 442
Size: 1753
Fragments arranged by size:
1753 1527 958 875 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4748 1239
Size: 3509 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2230 4213 5078 5376 2230
Size: 1983 865 298 2942
Fragments arranged by size:
2942 1983 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2373 3244 3879 493
Size: 711 1169 871 635 2702
Fragments arranged by size:
2702 1169 871 711 635
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3057 3895 4277 4912
Size: 53 83 186 936 1524 838 382 635
Cuts at: 4912 275
Size: 1451
Fragments arranged by size:
1524 1451 936 838 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4521 4919 5193 5340 5751 4521
Size: 398 274 147 411 4858
Fragments arranged by size:
4858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2927 2957 1178
Size: 30 165 30 1524 30 4309
Fragments arranged by size:
4309 1524 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2597 2906 1186
Size: 1099 312 309 4368
Fragments arranged by size:
4368 1099 312 309
BseYI C'CCAG_C
Cuts at: 5291 5787 5291
Size: 496 5592
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3308 1163
Size: 573 1572 3943
Fragments arranged by size:
3943 1572 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2322 2667 4149 4234 5305 2322
Size: 345 1482 85 1071 3105
Fragments arranged by size:
3105 1482 1071 345 85
BsmI GAATG_Cn'
Cuts at: 3344 3599 3692 3344
Size: 255 93 5740
Fragments arranged by size:
5740 255 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2254 2322 2634 2646 2667 3407 4149
Size: 399 464 68 312 12 21 740 742
Cuts at: 4149 4234 5305 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 740 464 399 312 85
68 21 12
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 844
Size: 2599 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4732 4919 1700
Size: 110 2922 187 2869
Fragments arranged by size:
2922 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 4148 5160 4148
Size: 1012 5076
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6088
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstXI CCAn_nnnn'nTGG
Cuts at: 2854 2854
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 3283 3418 3785 4170 4187 5639 5725 5737
Size: 1311 135 367 385 17 1452 86 12
Cuts at: 5737 6083 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1311 385 367 346 135 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5536
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 3015 3604 4398 4418 3015
Size: 589 794 20 4685
Fragments arranged by size:
4685 794 589 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DraIII CAC_nnn'GTG
Cuts at: 3244 3244
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2240 5095 809
Size: 507 924 2855 1802
Fragments arranged by size:
2855 1802 924 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2562 3257 3535
Size: 54 1425 50 275 18 732 695 278
Cuts at: 3535 4424 6053 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 732 695 278 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 2604 3359 4018 1353
Size: 764 487 755 659 3423
Fragments arranged by size:
3423 764 755 659 487
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2320 2665 2320
Size: 345 5743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2794 3107 3129 4151 4748 5535
Size: 393 492 670 313 22 1022 597 787
Cuts at: 5535 5803 1239
Size: 268 1524
Fragments arranged by size:
1524 1022 787 670 597 492 393 313
268 22
EcoNI CCTnn'n_nnAGG
Cuts at: 3122 3272 3122
Size: 150 5938
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2815 3000 2410
Size: 405 185 5498
Fragments arranged by size:
5498 405 185
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 5235 1678
Size: 3557 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2927 2959 4729 4761 4803
Size: 32 135 32 1355 32 1770 32 42
Cuts at: 4803 4835 1373
Size: 32 2626
Fragments arranged by size:
2626 1770 1355 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2797 3432 3678 669
Size: 988 281 859 635 246 3079
Fragments arranged by size:
3079 988 859 635 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2564 3259 10
Size: 54 1425 343 732 695 2839
Fragments arranged by size:
2839 1425 732 695 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2223 3332 4046 4405 4564 518
Size: 648 1057 1109 714 359 159 2042
Fragments arranged by size:
2042 1109 1057 714 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2364 3025 3504 4181 5329 5574 1606
Size: 758 661 479 677 1148 245 2120
Fragments arranged by size:
2120 1148 758 677 661 479 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5536
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NsiI A_TGCA'T
Cuts at: 2513 2513
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 3079 3446 4991 1132
Size: 97 1850 367 1545 2229
Fragments arranged by size:
2229 1850 1545 367 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2786 1108
Size: 1678 4410
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PmlI CAC'GTG
Cuts at: 2373 3244 2373
Size: 871 5217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PstI C_TGCA'G
Cuts at: 830 2619 3440 830
Size: 1789 821 3478
Fragments arranged by size:
3478 1789 821
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 3025 3025
Size: 6088
SacI G_AGCT'C
Cuts at: 2322 2667 2322
Size: 345 5743
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SapI GCTCTTCn'nnn_
Cuts at: 2117 2604 2117
Size: 487 5601
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 2615 3436 4571 5252 5443 826
Size: 219 1570 821 1135 681 191 1471
Fragments arranged by size:
1570 1471 1135 821 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3156 3415 1872
Size: 1284 259 4545
Fragments arranged by size:
4545 1284 259
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2245 4192 5093 5355 820
Size: 197 926 302 1947 901 262 1553
Fragments arranged by size:
1947 1553 926 901 302 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 2205 4012 5
Size: 47 2153 1807 2081
Fragments arranged by size:
2153 2081 1807 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2707 3527 513
Size: 552 1642 820 3074
Fragments arranged by size:
3074 1642 820 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2085 4115 4132 4285 4470 4915 325
Size: 1255 505 2030 17 153 185 445 1498
Fragments arranged by size:
2030 1498 1255 505 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4334 6046 96
Size: 274 80 33 51 1443 2357 1712 138
Fragments arranged by size:
2357 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 6048 6080 2163
Size: 32 3853 32 2171
Fragments arranged by size:
3853 2171 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2468 1316
Size: 1152 4936
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3029 3067 3029
Size: 38 6050
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3154 3413 1870
Size: 1284 259 4545
Fragments arranged by size:
4545 1284 259
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI