Restriction map for
pFN21AB3725
(Circular) (Six-base) MAPSORT of: pFN21AB3725.seq Check: 9663 from: 1 to: 8830
pFN21AB3725 8830 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6640 278
Size: 53 83 186 936 5104 2468
Fragments arranged by size:
5104 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2673 3799 4086 6173 1937
Size: 736 1126 287 2087 4594
Fragments arranged by size:
4594 2087 1126 736 287
Acc65I G'GTAC_C
Cuts at: 3531 4526 4909 6151 3531
Size: 995 383 1242 6210
Fragments arranged by size:
6210 1242 995 383
AclI AA'CG_TT
Cuts at: 1563 6957 7330 1563
Size: 5394 373 3063
Fragments arranged by size:
5394 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 3147 3536 4220 5130 5259 5879 5952
Size: 977 538 389 684 910 129 620 73
Cuts at: 5952 6024 6893 8277 8545 1632
Size: 72 869 1384 268 1917
Fragments arranged by size:
1917 1384 977 910 869 684 620 538
389 268 129 73 72
AflII C'TTAA_G
Cuts at: 820 1017 5864 820
Size: 197 4847 3786
Fragments arranged by size:
4847 3786 197
AflIII A'CryG_T
Cuts at: 1203 6041 7729 8706 1203
Size: 4838 1688 977 1327
Fragments arranged by size:
4838 1688 1327 977
AgeI A'CCGG_T
Cuts at: 5721 7661 5721
Size: 1940 6890
AhdI GACnn_n'nnGTC
Cuts at: 4597 7559 4597
Size: 2962 5868
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8830
AlwNI CAG_nnn'CTG
Cuts at: 2166 2587 3926 4168 4225 4772 6573 8145 2166
Size: 421 1339 242 57 547 1801 1572 2851
Fragments arranged by size:
2851 1801 1572 1339 547 421 242 57
ApaLI G'TGCA_C
Cuts at: 2620 6085 6887 8043 2620
Size: 3465 802 1156 3407
Fragments arranged by size:
3465 3407 1156 802
ApoI r'AATT_y
Cuts at: 1493 2898 3811 3851 5043 5077 5294 5883 5911
Size: 1405 913 40 1192 34 217 589 28
Cuts at: 5911 6139 6365 6517 8626 1493
Size: 228 226 152 2109 1697
Fragments arranged by size:
2109 1697 1405 1192 913 589 228 226
217 152 40 34 28
AseI AT'TA_AT
Cuts at: 160 1032 3303 3689 4437 7384 160
Size: 872 2271 386 748 2947 1606
Fragments arranged by size:
2947 2271 1606 872 748 386
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8830
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 2078 2221 2235 6155 1870
Size: 73 116 19 143 14 3920 4545
Fragments arranged by size:
4545 3920 143 116 73 19 14
BamHI G'GATC_C
Cuts at: 5175 6160 6527 5175
Size: 985 367 7478
Fragments arranged by size:
7478 985 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2375 3531 4526 4543 4909
Size: 325 217 627 588 1156 995 17 366
Cuts at: 4909 6151 7606 618
Size: 1242 1455 1842
Fragments arranged by size:
1842 1455 1242 1156 995 627 588 366
325 217 17
BanII G_rGCy'C
Cuts at: 1391 2715 3360 4232 6149 1391
Size: 1324 645 872 1917 4072
Fragments arranged by size:
4072 1917 1324 872 645
BbeI G_GCGC'C
Cuts at: 2379 2379
Size: 8830
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3380 3524 5193 5583 5638 5985 928
Size: 971 1481 144 1669 390 55 347 3773
Fragments arranged by size:
3773 1669 1481 971 390 347 144 55
BbvCI CC'TCA_GC
Cuts at: 5138 5138
Size: 8830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7021 7055 1903
Size: 34 5084 34 3678
Fragments arranged by size:
5084 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6722 7932 6722
Size: 1210 7620
BclI T'GATC_A
Cuts at: 1548 2292 5569 1548
Size: 744 3277 4809
Fragments arranged by size:
4809 3277 744
BfrBI ATG'CAT
Cuts at: 3071 3071
Size: 8830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7441 136
Size: 107 122 71 631 605 5769 1525
Fragments arranged by size:
5769 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4461 8825 4461
Size: 4364 4466
BlpI GC'TnA_GC
Cuts at: 6247 6247
Size: 8830
Bme1580I G_kGCm'C
Cuts at: 1790 2624 6089 6891 8047 1790
Size: 834 3465 802 1156 2573
Fragments arranged by size:
3465 2573 1156 834 802
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2634 3439 4664
Size: 416 53 83 399 24 1217 805 1225
Cuts at: 4664 6561 7519 442
Size: 1897 958 1753
Fragments arranged by size:
1897 1753 1225 1217 958 805 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8830
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4969 4969
Size: 8830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3404 4037 4299 4517 5372 7490 1239
Size: 2165 633 262 218 855 2118 2579
Fragments arranged by size:
2579 2165 2118 855 633 262 218
Bpu10I CC'TnA_GC
Cuts at: 4851 5138 5229 4851
Size: 287 91 8452
Fragments arranged by size:
8452 287 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3521 4123 4442 6082 6955 7820 8118 3521
Size: 602 319 1640 873 865 298 4233
Fragments arranged by size:
4233 1640 873 865 602 319 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3234 4605 7493 882
Size: 617 1735 1371 2888 2219
Fragments arranged by size:
2888 2219 1735 1371 617
BsaAI yAC'GTr
Cuts at: 493 1204 3775 6044 6621 493
Size: 711 2571 2269 577 2702
Fragments arranged by size:
2702 2571 2269 711 577
BsaBI GATnn'nnATC
Cuts at: 8824 8824
Size: 8830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2376 6637 7019 7654
Size: 53 83 186 936 843 4261 382 635
Cuts at: 7654 275
Size: 1451
Fragments arranged by size:
4261 1451 936 843 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3360 5721 7263 7661 7935 8082 8493 3360
Size: 2361 1542 398 274 147 411 3697
Fragments arranged by size:
3697 2361 1542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5796 5826 1178
Size: 30 165 30 4393 30 4182
Fragments arranged by size:
4393 4182 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2118 2144 2209 3308 5088 1186
Size: 932 26 65 1099 1780 4928
Fragments arranged by size:
4928 1780 1099 932 65 26
BseYI C'CCAG_C
Cuts at: 8033 8529 8033
Size: 496 8334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2053 1163
Size: 573 317 7940
Fragments arranged by size:
7940 573 317
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6280 7041 8069 1540
Size: 275 186 4279 761 1028 2301
Fragments arranged by size:
4279 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2136 2624 2715 3360 5098 5681 5719 6089
Size: 124 488 91 645 1738 583 38 370
Cuts at: 6089 6891 6976 8047 2012
Size: 802 85 1071 2795
Fragments arranged by size:
2795 1738 1071 802 645 583 488 370
124 91 85 38
BsmI GAATG_Cn'
Cuts at: 4742 5064 6341 6434 4742
Size: 322 1277 93 7138
Fragments arranged by size:
7138 1277 322 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2136 2624 2715 3360 4232 5098
Size: 399 222 124 488 91 645 872 866
Cuts at: 5098 5681 5719 6089 6149 6891 6976 8047 1391
Size: 583 38 370 60 742 85 1071 2174
Fragments arranged by size:
2174 1071 872 866 742 645 583 488
399 370 222 124 91 85 60 38
BspEI T'CCGG_A
Cuts at: 3360 8493 3360
Size: 5133 3697
BspHI T'CATG_A
Cuts at: 2694 5701 6719 8449 2694
Size: 3007 1018 1730 3075
Fragments arranged by size:
3075 3007 1730 1018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4082 4261 6185 844
Size: 3238 179 1924 3489
Fragments arranged by size:
3489 3238 1924 179
BsrBI CCG'CTC
Cuts at: 1394 1823 2108 6717 1394
Size: 429 285 4609 3507
Fragments arranged by size:
4609 3507 429 285
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 5277 7325 7499 65
Size: 1183 2936 1093 2048 174 1396
Fragments arranged by size:
2936 2048 1396 1183 1093 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4906 5721 5991 7474 7661 1700
Size: 110 3096 815 270 1483 187 2869
Fragments arranged by size:
3096 2869 1483 815 270 187 110
BsrGI T'GTAC_A
Cuts at: 96 3917 4610 5734 96
Size: 3821 693 1124 3192
Fragments arranged by size:
3821 3192 1124 693
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8830
BssSI C'ACGA_G
Cuts at: 6890 7902 6890
Size: 1012 7818
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2140 2350 2456 794
Size: 1346 210 106 7168
Fragments arranged by size:
7168 1346 210 106
BstBI TT'CG_AA
Cuts at: 6115 7634 6115
Size: 1519 7311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8830
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 2431
Size: 8830
BstYI r'GATC_y
Cuts at: 1972 4461 4710 4768 5175 5303 6160 6527 6912
Size: 2489 249 58 407 128 857 367 385
Cuts at: 6912 6929 8381 8467 8479 8825 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2489 1977 1452 857 407 385 367 346
249 128 86 58 17 12
BstZ17I GTA'TAC
Cuts at: 3800 3800
Size: 8830
Bsu36I CC'TnA_GG
Cuts at: 5637 5668 5637
Size: 31 8799
BtgI C'CryG_G
Cuts at: 513 1065 2277 3135 3244 5371 513
Size: 552 1212 858 109 2127 3972
Fragments arranged by size:
3972 2127 1212 858 552 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2490 487
Size: 683 56 1264 6827
Fragments arranged by size:
6827 1264 683 56
BtsI GCAGTG_nn'
Cuts at: 2233 4948 6346 7140 7160 2233
Size: 2715 1398 794 20 3903
Fragments arranged by size:
3903 2715 1398 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8795
DraI TTT'AAA
Cuts at: 3511 4696 4818 6134 3511
Size: 1185 122 1316 6207
Fragments arranged by size:
6207 1316 1185 122
DraIII CAC_nnn'GTG
Cuts at: 3055 6093 3055
Size: 3038 5792
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7837 809
Size: 507 6521 1802
Fragments arranged by size:
6521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2325 2535
Size: 54 1425 50 275 18 381 114 210
Cuts at: 2535 3603 4626 6277 7166 8795 8
Size: 1068 1023 1651 889 1629 43
Fragments arranged by size:
1651 1629 1425 1068 1023 889 381 275
210 114 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6277 1537
Size: 275 4465 4090
Fragments arranged by size:
4465 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3829 3963 6760 1353
Size: 2476 134 2797 3423
Fragments arranged by size:
3423 2797 2476 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 7417 7791 7937 387
Size: 1064 138 444 5384 374 146 1280
Fragments arranged by size:
5384 1280 1064 444 374 146 138
EcoICRI GAG'CTC
Cuts at: 2713 3358 2713
Size: 645 8185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2609 3147 3404 3536 4037 4220 4299
Size: 393 977 538 257 132 501 183 79
Cuts at: 4299 4517 5130 5259 5372 5879 5952 6024 6893
Size: 218 613 129 113 507 73 72 869
Cuts at: 6893 7490 8277 8545 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 977 869 787 613 597 538 507
501 393 268 257 218 183 132 129
113 79 73 72
EcoNI CCTnn'n_nnAGG
Cuts at: 3644 3644
Size: 8830
EcoO109I rG'GnC_Cy
Cuts at: 2338 4273 2338
Size: 1935 6895
EcoRI G'AATT_C
Cuts at: 2898 5043 6139 6517 2898
Size: 2145 1096 378 5211
Fragments arranged by size:
5211 2145 1096 378
EcoRV GAT'ATC
Cuts at: 2974 2974
Size: 8830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3424 5831 3424
Size: 2407 6423
FspI TGC'GCA
Cuts at: 7336 7336
Size: 8830
HaeII r_GCGC'y
Cuts at: 1678 2184 2379 7977 1678
Size: 506 195 5598 2531
Fragments arranged by size:
5598 2531 506 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 3026 3058 4583
Size: 32 135 32 980 32 442 32 1525
Cuts at: 4583 4615 4709 4741 4979 5011 7471 7503 7545
Size: 32 94 32 238 32 2460 32 42
Cuts at: 7545 7577 1373
Size: 32 2626
Fragments arranged by size:
2626 2460 1525 980 442 238 135 94
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2706 6174 6420 669
Size: 988 281 768 3468 246 3079
Fragments arranged by size:
3468 3079 988 768 281 246
HindIII A'AGCT_T
Cuts at: 748 3503 4853 5861 748
Size: 2755 1350 1008 3717
Fragments arranged by size:
3717 2755 1350 1008
HpaI GTT'AAC
Cuts at: 6420 6420
Size: 8830
KasI G'GCGC_C
Cuts at: 2375 2375
Size: 8830
KpnI G_GTAC'C
Cuts at: 3535 4530 4913 6155 3535
Size: 995 383 1242 6210
Fragments arranged by size:
6210 1242 995 383
MfeI C'AATT_G
Cuts at: 4341 6429 4341
Size: 2088 6742
MluI A'CGCG_T
Cuts at: 8706 8706
Size: 8830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3418 7944 8128 1305
Size: 2113 4526 184 2007
Fragments arranged by size:
4526 2113 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2537 3605 4628 10
Size: 54 1425 343 705 1068 1023 4212
Fragments arranged by size:
4212 1425 1068 1023 705 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3051 3526 4622 6788 7147 7306 518
Size: 648 1885 475 1096 2166 359 159 2042
Fragments arranged by size:
2166 2042 1885 1096 648 475 359 159
MspA1I CmG'CkG
Cuts at: 1606 2140 2186 2885 3329 4871 6246 6923 8071
Size: 534 46 699 444 1542 1375 677 1148
Cuts at: 8071 8316 1606
Size: 245 2120
Fragments arranged by size:
2120 1542 1375 1148 699 677 534 444
245 46
NarI GG'CG_CC
Cuts at: 2376 2376
Size: 8830
NcoI C'CATG_G
Cuts at: 513 1065 3135 3244 5371 513
Size: 552 2070 109 2127 3972
Fragments arranged by size:
3972 2127 2070 552 109
NdeI CA'TA_TG
Cuts at: 387 2287 387
Size: 1900 6930
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8830
NotI GC'GGCC_GC
Cuts at: 6277 6277
Size: 8830
NsiI A_TGCA'T
Cuts at: 3073 3073
Size: 8830
NspI r_CATG'y
Cuts at: 1132 1229 6188 7733 1132
Size: 97 4959 1545 2229
Fragments arranged by size:
4959 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8830
PciI A'CATG_T
Cuts at: 7729 7729
Size: 8830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4225 4540 1108
Size: 3117 315 5398
Fragments arranged by size:
5398 3117 315
PfoI T'CCnGG_A
Cuts at: 2820 2976 2820
Size: 156 8674
PmeI GTTT'AAAC
Cuts at: 6134 6134
Size: 8830
PmlI CAC'GTG
Cuts at: 3775 6044 3775
Size: 2269 6561
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6899 6931 8412 8444 6899
Size: 32 1481 32 7285
Fragments arranged by size:
7285 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4273 4273
Size: 8830
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8830
PsiI TTA'TAA
Cuts at: 6400 6400
Size: 8830
PspXI vC'TCGA_Gb
Cuts at: 2221 2221
Size: 8830
PstI C_TGCA'G
Cuts at: 830 2096 2346 2583 4732 6182 830
Size: 1266 250 237 2149 1450 3478
Fragments arranged by size:
3478 2149 1450 1266 250 237
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8830
PvuII CAG'CTG
Cuts at: 2140 2885 3329 4871 2140
Size: 745 444 1542 6099
Fragments arranged by size:
6099 1542 745 444
SacI G_AGCT'C
Cuts at: 2715 3360 2715
Size: 645 8185
SalI G'TCGA_C
Cuts at: 1936 6172 1936
Size: 4236 4594
SbfI CC_TGCA'GG
Cuts at: 6182 6182
Size: 8830
ScaI AGT'ACT
Cuts at: 2816 7078 8790 2816
Size: 4262 1712 2856
Fragments arranged by size:
4262 2856 1712
SfcI C'TryA_G
Cuts at: 826 1045 2092 2342 2579 2965 3290 4444 4728
Size: 219 1047 250 237 386 325 1154 284
Cuts at: 4728 6178 7313 7994 8185 826
Size: 1450 1135 681 191 1471
Fragments arranged by size:
1471 1450 1154 1135 1047 681 386 325
284 250 237 219 191
SfoI GGC'GCC
Cuts at: 2377 2377
Size: 8830
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8830
SmaI CCC'GGG
Cuts at: 1872 6157 1872
Size: 4285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2221 3536 4102 4457 5864 6061
Size: 197 926 278 1315 566 355 1407 197
Cuts at: 6061 6934 7835 8097 820
Size: 873 901 262 1553
Fragments arranged by size:
1553 1407 1315 926 901 873 566 355
278 262 197 197
SnaBI TAC'GTA
Cuts at: 493 6621 493
Size: 6128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8830
SphI G_CATG'C
Cuts at: 1132 6188 1132
Size: 5056 3774
SspI AAT'ATT
Cuts at: 5 52 3693 4213 6754 5
Size: 47 3641 520 2541 2081
Fragments arranged by size:
3641 2541 2081 520 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8830
StyI C'CwwG_G
Cuts at: 513 1065 2308 2509 3135 3244 4737 5371 6269
Size: 552 1243 201 626 109 1493 634 898
Cuts at: 6269 513
Size: 3074
Fragments arranged by size:
3074 1493 1243 898 634 626 552 201
109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5328 6857 6874 7027 7212 7657 325
Size: 1255 3748 1529 17 153 185 445 1498
Fragments arranged by size:
3748 1529 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2814 3917 4508
Size: 274 80 33 51 1443 837 1103 591
Cuts at: 4508 4610 5734 7076 8788 96
Size: 102 1124 1342 1712 138
Fragments arranged by size:
1712 1443 1342 1124 1103 837 591 274
138 102 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2626 2658 3802 3834 5134 5166 8790 8822 2626
Size: 32 1144 32 1300 32 3624 32 2634
Fragments arranged by size:
3624 2634 1300 1144 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4052 1316
Size: 2736 6094
XbaI T'CTAG_A
Cuts at: 6166 6166
Size: 8830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5512 5770 5512
Size: 258 8572
XhoI C'TCGA_G
Cuts at: 1943 2221 1943
Size: 278 8552
XmaI C'CCGG_G
Cuts at: 1870 6155 1870
Size: 4285 4545
XmnI GAAnn'nnTTC
Cuts at: 4247 4288 5784 6959 4247
Size: 41 1496 1175 6118
Fragments arranged by size:
6118 1496 1175 41
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6638 276
Size: 53 83 186 936 5104 2468
Fragments arranged by size:
5104 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI
BsmBI FseI FspAI NaeI NgoMIV NruI PacI PspOMI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI