Restriction map for
pFN21AB3720
(Circular) (Six-base) MAPSORT of: pFN21AB3720.seq Check: 923 from: 1 to: 5677
pFN21AB3720 5677 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3740 5124 5392 1632
Size: 2108 1384 268 1917
Fragments arranged by size:
2108 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 4576 5553 1203
Size: 3373 977 1327
Fragments arranged by size:
3373 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 2169 2750 1166
Size: 1003 581 4093
Fragments arranged by size:
4093 1003 581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2175 1184
Size: 991 4686
AlwNI CAG_nnn'CTG
Cuts at: 2563 3420 4992 2563
Size: 857 1572 3248
Fragments arranged by size:
3248 1572 857
ApaI G_GGCC'C
Cuts at: 2947 2947
Size: 5677
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2381 2986 3212 3364 5473 1493
Size: 888 605 226 152 2109 1697
Fragments arranged by size:
2109 1697 888 605 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2640 3002 1870
Size: 73 697 362 4545
Fragments arranged by size:
4545 697 362 73
AvrII C'CTAG_G
Cuts at: 2963 2963
Size: 5677
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2744 2847 2998 4453 618
Size: 325 217 627 957 103 151 1455 1842
Fragments arranged by size:
1842 1455 957 627 325 217 151 103
BanII G_rGCy'C
Cuts at: 1391 2882 2947 2996 1391
Size: 1491 65 49 4072
Fragments arranged by size:
4072 1491 65 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4706
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3868 3902 1903
Size: 34 50 34 1847 34 3678
Fragments arranged by size:
3678 1847 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 2717 4288
Size: 107 122 71 631 605 400 645 1571
Cuts at: 4288 136
Size: 1525
Fragments arranged by size:
1571 1525 645 631 605 400 122 107
71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 2090 2620 2641 2747 2802 2947 3738 4894
Size: 300 530 21 106 55 145 791 1156
Cuts at: 4894 1790
Size: 2573
Fragments arranged by size:
2573 1156 791 530 300 145 106 55
21
BmgBI CAC'GTC
Cuts at: 1318 2859 1318
Size: 1541 4136
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2350 2909 3408
Size: 416 53 83 399 24 933 559 499
Cuts at: 3408 4366 442
Size: 958 1753
Fragments arranged by size:
1753 958 933 559 499 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2262 2672 1056
Size: 1206 410 4061
Fragments arranged by size:
4061 1206 410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2188 2303 2757 4337 1239
Size: 949 115 454 1580 2579
Fragments arranged by size:
2579 1580 949 454 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3802 4667 4965 3802
Size: 865 298 4514
Fragments arranged by size:
4514 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2626 3468 493
Size: 711 1422 842 2702
Fragments arranged by size:
2702 1422 842 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3484 3866 4501 275
Size: 53 83 186 936 1951 382 635 1451
Fragments arranged by size:
1951 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2139 2913 4110 4508 4782 4929 5340 2139
Size: 774 1197 398 274 147 411 2476
Fragments arranged by size:
2476 1197 774 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2474 2504 1178
Size: 30 165 30 1071 30 4351
Fragments arranged by size:
4351 1071 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2256 2720 1186
Size: 1042 28 464 4143
Fragments arranged by size:
4143 1042 464 28
BseYI C'CCAG_C
Cuts at: 2549 2567 2599 2940 4880 5376 2549
Size: 18 32 341 1940 496 2850
Fragments arranged by size:
2850 1940 496 341 32 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2145 2212 1163
Size: 573 409 67 4628
Fragments arranged by size:
4628 573 409 67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3738 3823 4894 3738
Size: 85 1071 4521
Fragments arranged by size:
4521 1071 85
BsmI GAATG_Cn'
Cuts at: 2426 3188 3281 2426
Size: 762 93 4822
Fragments arranged by size:
4822 762 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2620 2641 2747 2802 2882 2947
Size: 399 300 530 21 106 55 80 65
Cuts at: 2947 2996 3738 3823 4894 1391
Size: 49 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 530 399 300 106 85
80 65 55 49 21
BspEI T'CCGG_A
Cuts at: 2913 5340 2913
Size: 2427 3250
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2485 3564 1394
Size: 429 662 1079 3507
Fragments arranged by size:
3507 1079 662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2823 4172 4346 65
Size: 1183 1575 1349 174 1396
Fragments arranged by size:
1575 1396 1349 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4321 4508 1700
Size: 110 2511 187 2869
Fragments arranged by size:
2869 2511 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 2016 1925
Size: 91 5586
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2501 2035
Size: 466 5211
BstYI r'GATC_y
Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672
Size: 1035 367 385 17 1452 86 12 346
Cuts at: 5672 1972
Size: 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2349 2539 2802 513
Size: 552 1284 190 263 3388
Fragments arranged by size:
3388 1284 552 263 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2443 487
Size: 683 56 1217 3721
Fragments arranged by size:
3721 1217 683 56
BtsI GCAGTG_nn'
Cuts at: 2224 3193 3987 4007 2224
Size: 969 794 20 3894
Fragments arranged by size:
3894 969 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3124 4013 5642
Size: 54 1425 50 275 18 1294 889 1629
Cuts at: 5642 8
Size: 43
Fragments arranged by size:
1629 1425 1294 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3607 1353
Size: 2254 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2303 2757 3740 4337 5124 5392
Size: 393 556 115 454 983 597 787 268
Cuts at: 5392 1239
Size: 1524
Fragments arranged by size:
1524 983 787 597 556 454 393 268
115
EcoO109I rG'GnC_Cy
Cuts at: 2560 2719 2927 2560
Size: 159 208 5310
Fragments arranged by size:
5310 208 159
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
FspI TGC'GCA
Cuts at: 2294 4183 2294
Size: 1889 3788
HaeII r_GCGC'y
Cuts at: 1678 4824 1678
Size: 3146 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2188 2220 2496 2528 4318
Size: 32 135 32 616 32 276 32 1790
Cuts at: 4318 4350 4392 4424 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1790 616 276 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3267 669
Size: 988 281 1083 246 3079
Fragments arranged by size:
3079 1083 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2472 4791 4975 1305
Size: 1167 2319 184 2007
Fragments arranged by size:
2319 2007 1167 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3855
Fragments arranged by size:
3855 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2169 2474 2750 3635 3994 4153 518
Size: 648 1003 305 276 885 359 159 2042
Fragments arranged by size:
2042 1003 885 648 359 305 276 159
MspA1I CmG'CkG
Cuts at: 1606 2844 3093 3770 4918 5163 1606
Size: 1238 249 677 1148 245 2120
Fragments arranged by size:
2120 1238 1148 677 249 245
NcoI C'CATG_G
Cuts at: 513 1065 2539 513
Size: 552 1474 3651
Fragments arranged by size:
3651 1474 552
NdeI CA'TA_TG
Cuts at: 387 2701 2814 387
Size: 2314 113 3250
Fragments arranged by size:
3250 2314 113
NheI G'CTAG_C
Cuts at: 1052 2258 2668 1052
Size: 1206 410 4061
Fragments arranged by size:
4061 1206 410
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 3035 4580 1132
Size: 97 1806 1545 2229
Fragments arranged by size:
2229 1806 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2371 1108
Size: 1263 4414
PfoI T'CCnGG_A
Cuts at: 2649 2649
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2927 2927
Size: 5677
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PspOMI G'GGCC_C
Cuts at: 2943 2943
Size: 5677
PstI C_TGCA'G
Cuts at: 830 2559 2682 3029 830
Size: 1729 123 347 3478
Fragments arranged by size:
3478 1729 347 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2844 2844
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SbfI CC_TGCA'GG
Cuts at: 2559 3029 2559
Size: 470 5207
ScaI AGT'ACT
Cuts at: 2300 3925 5637 2300
Size: 1625 1712 2340
Fragments arranged by size:
2340 1712 1625
SexAI A'CCwGG_T
Cuts at: 2112 2112
Size: 5677
SfcI C'TryA_G
Cuts at: 826 1045 2555 2678 2839 3025 4160 4841 5032
Size: 219 1510 123 161 186 1135 681 191
Cuts at: 5032 826
Size: 1471
Fragments arranged by size:
1510 1471 1135 681 219 191 186 161
123
SfiI GGCCn_nnn'nGGCC
Cuts at: 2717 2717
Size: 5677
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3781 4682 4944 820
Size: 197 926 1838 901 262 1553
Fragments arranged by size:
1838 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5677
StyI C'CwwG_G
Cuts at: 513 1065 2386 2539 2748 2963 3116 513
Size: 552 1321 153 209 215 153 3074
Fragments arranged by size:
3074 1321 552 215 209 153 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2890 3704 3721 3874 4059 4504 325
Size: 1255 1310 814 17 153 185 445 1498
Fragments arranged by size:
1498 1310 1255 814 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2176 2298 3923
Size: 274 80 33 51 1443 199 122 1625
Cuts at: 3923 5635 96
Size: 1712 138
Fragments arranged by size:
1712 1625 1443 274 199 138 122 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2330 2362 2474 2506 5637 5669 2330
Size: 32 112 32 3131 32 2338
Fragments arranged by size:
3131 2338 112 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2896 2896
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SfoI SrfI SwaI