Restriction map for
pFN21AB3715
(Circular) (Six-base) MAPSORT of: pFN21AB3715.seq Check: 590 from: 1 to: 5329
pFN21AB3715 5329 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2087 2337 3139 278
Size: 53 83 186 936 551 250 802 2468
Fragments arranged by size:
2468 936 802 551 250 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2672 1937
Size: 735 4594
Acc65I G'GTAC_C
Cuts at: 2650 2650
Size: 5329
AclI AA'CG_TT
Cuts at: 1563 3456 3829 1563
Size: 1893 373 3063
Fragments arranged by size:
3063 1893 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3392 4776 5044 1632
Size: 1760 1384 268 1917
Fragments arranged by size:
1917 1760 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5132
AflIII A'CryG_T
Cuts at: 1203 2509 4228 5205 1203
Size: 1306 1719 977 1327
Fragments arranged by size:
1719 1327 1306 977
AgeI A'CCGG_T
Cuts at: 4160 4160
Size: 5329
AhdI GACnn_n'nnGTC
Cuts at: 2155 4058 2155
Size: 1903 3426
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5329
AlwNI CAG_nnn'CTG
Cuts at: 3072 4644 3072
Size: 1572 3757
ApaLI G'TGCA_C
Cuts at: 3386 4542 3386
Size: 1156 4173
ApoI r'AATT_y
Cuts at: 1493 2042 2638 2864 3016 5125 1493
Size: 549 596 226 152 2109 1697
Fragments arranged by size:
2109 1697 596 549 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3883 160
Size: 872 2851 1606
Fragments arranged by size:
2851 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
AvaI C'yCGr_G
Cuts at: 1870 1943 2654 1870
Size: 73 711 4545
Fragments arranged by size:
4545 711 73
BamHI G'GATC_C
Cuts at: 2659 3026 2659
Size: 367 4962
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2650 4105 618
Size: 325 217 627 863 1455 1842
Fragments arranged by size:
1842 1455 863 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2601 2648 1391
Size: 1210 47 4072
Fragments arranged by size:
4072 1210 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2087 2483 928
Size: 971 188 396 3774
Fragments arranged by size:
3774 971 396 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2486 2520 3520 3554 1903
Size: 34 549 34 1000 34 3678
Fragments arranged by size:
3678 1000 549 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3221 4431 3221
Size: 1210 4119
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5329
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3940 136
Size: 107 122 71 631 605 2268 1525
Fragments arranged by size:
2268 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5324 5324
Size: 5329
BlpI GC'TnA_GC
Cuts at: 2746 2746
Size: 5329
Bme1580I G_kGCm'C
Cuts at: 1790 3390 4546 1790
Size: 1600 1156 2573
Fragments arranged by size:
2573 1600 1156
BmgBI CAC'GTC
Cuts at: 1318 2510 1318
Size: 1192 4137
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3060 4018 442
Size: 416 53 83 399 24 1643 958 1753
Fragments arranged by size:
1753 1643 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3989 1239
Size: 2750 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3454 4319 4617 3454
Size: 865 298 4166
Fragments arranged by size:
4166 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2459 3992 882
Size: 617 960 1533 2219
Fragments arranged by size:
2219 1533 960 617
BsaAI yAC'GTr
Cuts at: 493 1204 3120 493
Size: 711 1916 2702
Fragments arranged by size:
2702 1916 711
BsaBI GATnn'nnATC
Cuts at: 2414 2573 5323 2414
Size: 159 2750 2420
Fragments arranged by size:
2750 2420 159
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2084 2334 2516 3136
Size: 53 83 186 936 551 250 182 620
Cuts at: 3136 3518 4153 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 620 551 382 250 186
182 83 53
BsaWI w'CCGG_w
Cuts at: 3762 4160 4434 4581 4992 3762
Size: 398 274 147 411 4099
Fragments arranged by size:
4099 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5104
Fragments arranged by size:
5104 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 1186
Size: 1249 4080
BseYI C'CCAG_C
Cuts at: 2243 4532 5028 2243
Size: 2289 496 2544
Fragments arranged by size:
2544 2289 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4756
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2779 3540 4568 1540
Size: 275 186 778 761 1028 2301
Fragments arranged by size:
2301 1028 778 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3390 3475 4546 3390
Size: 85 1071 4173
Fragments arranged by size:
4173 1071 85
BsmI GAATG_Cn'
Cuts at: 2840 2933 2840
Size: 93 5236
BsmBI CGTCTCn'nnnn_
Cuts at: 2091 2091
Size: 5329
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2601 2648 3390 3475 4546 1391
Size: 399 811 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 811 742 399 85 47
BspEI T'CCGG_A
Cuts at: 4992 4992
Size: 5329
BspHI T'CATG_A
Cuts at: 3218 4948 3218
Size: 1730 3599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2684 844
Size: 1840 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3216 1394
Size: 429 1393 3507
Fragments arranged by size:
3507 1393 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3824 3998 65
Size: 1183 2576 174 1396
Fragments arranged by size:
2576 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3973 4160 1700
Size: 110 2163 187 2869
Fragments arranged by size:
2869 2163 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5329
BssHII G'CGCG_C
Cuts at: 1925 2434 1925
Size: 509 4820
BssSI C'ACGA_G
Cuts at: 3389 4401 3389
Size: 1012 4317
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5329
BstBI TT'CG_AA
Cuts at: 4133 4133
Size: 5329
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5329
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2173
Size: 5329
BstYI r'GATC_y
Cuts at: 1972 2659 3026 3411 3428 4880 4966 4978 5324
Size: 687 367 385 17 1452 86 12 346
Cuts at: 5324 1972
Size: 1977
Fragments arranged by size:
1977 1452 687 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2262 513
Size: 552 1197 3580
Fragments arranged by size:
3580 1197 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4590
Fragments arranged by size:
4590 683 56
BtsI GCAGTG_nn'
Cuts at: 2359 2845 3639 3659 2359
Size: 486 794 20 4029
Fragments arranged by size:
4029 794 486 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5329
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5294
DraI TTT'AAA
Cuts at: 2633 2633
Size: 5329
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4336 809
Size: 507 3020 1802
Fragments arranged by size:
3020 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2604 2776 3665
Size: 54 1425 50 275 18 774 172 889
Cuts at: 3665 5294 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 774 275 172 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2776 1537
Size: 275 964 4090
Fragments arranged by size:
4090 964 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3259 1353
Size: 1906 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3916 4290 4436 387
Size: 1064 138 2327 374 146 1280
Fragments arranged by size:
2327 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3392 3989 4776 5044 1239
Size: 393 1760 597 787 268 1524
Fragments arranged by size:
1760 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2638 3016 2638
Size: 378 4951
FspI TGC'GCA
Cuts at: 3835 3835
Size: 5329
HaeII r_GCGC'y
Cuts at: 1678 4476 1678
Size: 2798 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 2184 2216 3970
Size: 32 135 32 569 32 11 32 1754
Cuts at: 3970 4002 4044 4076 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1754 569 135 42 32 32 32
32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2673 2919 669
Size: 988 281 735 246 3079
Fragments arranged by size:
3079 988 735 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5329
HpaI GTT'AAC
Cuts at: 2919 2919
Size: 5329
KpnI G_GTAC'C
Cuts at: 2654 2654
Size: 5329
MfeI C'AATT_G
Cuts at: 2928 2928
Size: 5329
MluI A'CGCG_T
Cuts at: 5205 5205
Size: 5329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2332 4443 4627 1305
Size: 1027 2111 184 2007
Fragments arranged by size:
2111 2007 1027 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3507
Fragments arranged by size:
3507 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2372 3287 3646 3805 518
Size: 648 1206 915 359 159 2042
Fragments arranged by size:
2042 1206 915 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2213 2745 3422 4570 4815 1606
Size: 607 532 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 607 532 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4777
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5329
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5329
NotI GC'GGCC_GC
Cuts at: 2776 2776
Size: 5329
NspI r_CATG'y
Cuts at: 1132 1229 2687 4232 1132
Size: 97 1458 1545 2229
Fragments arranged by size:
2229 1545 1458 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5329
PciI A'CATG_T
Cuts at: 4228 4228
Size: 5329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5329
PfoI T'CCnGG_A
Cuts at: 2253 2544 2253
Size: 291 5038
PmeI GTTT'AAAC
Cuts at: 2633 2633
Size: 5329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 4911 4943 3398
Size: 32 1481 32 3784
Fragments arranged by size:
3784 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5329
PsiI TTA'TAA
Cuts at: 2899 2899
Size: 5329
PstI C_TGCA'G
Cuts at: 830 2199 2681 830
Size: 1369 482 3478
Fragments arranged by size:
3478 1369 482
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5329
PvuII CAG'CTG
Cuts at: 2213 2213
Size: 5329
SalI G'TCGA_C
Cuts at: 1936 2671 1936
Size: 735 4594
SbfI CC_TGCA'GG
Cuts at: 2681 2681
Size: 5329
ScaI AGT'ACT
Cuts at: 3577 5289 3577
Size: 1712 3617
SfcI C'TryA_G
Cuts at: 826 1045 2195 2677 3812 4493 4684 826
Size: 219 1150 482 1135 681 191 1471
Fragments arranged by size:
1471 1150 1135 681 482 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5329
SmaI CCC'GGG
Cuts at: 1872 2656 1872
Size: 784 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3433 4334 4596 820
Size: 197 926 1490 901 262 1553
Fragments arranged by size:
1553 1490 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3120 493
Size: 2627 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5329
SphI G_CATG'C
Cuts at: 1132 2687 1132
Size: 1555 3774
SspI AAT'ATT
Cuts at: 5 52 3253 5
Size: 47 3201 2081
Fragments arranged by size:
3201 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5329
StyI C'CwwG_G
Cuts at: 513 1065 2768 513
Size: 552 1703 3074
Fragments arranged by size:
3074 1703 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3356 3373 3526 3711 4156 325
Size: 1255 1776 17 153 185 445 1498
Fragments arranged by size:
1776 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2169 3575 5287
Size: 274 80 33 51 1443 192 1406 1712
Cuts at: 5287 96
Size: 138
Fragments arranged by size:
1712 1443 1406 274 192 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5289 5321 5289
Size: 32 5297
Tth111I GACn'n_nGTC
Cuts at: 1316 2333 1316
Size: 1017 4312
XbaI T'CTAG_A
Cuts at: 2665 2665
Size: 5329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2138 2269 2138
Size: 131 5198
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5329
XmaI C'CCGG_G
Cuts at: 1870 2654 1870
Size: 784 4545
XmnI GAAnn'nnTTC
Cuts at: 3458 3458
Size: 5329
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2085 2335 3137 276
Size: 53 83 186 936 551 250 802 2468
Fragments arranged by size:
2468 936 802 551 250 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI