Restriction map for
pFN21AB3711
(Circular) (Six-base) MAPSORT of: pFN21AB3711.seq Check: 3007 from: 1 to: 4930
pFN21AB3711 4930 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2740 278
Size: 53 83 186 936 1204 2468
Fragments arranged by size:
2468 1204 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2273 1937
Size: 336 4594
Acc65I G'GTAC_C
Cuts at: 2251 2251
Size: 4930
AclI AA'CG_TT
Cuts at: 1563 3057 3430 1563
Size: 1494 373 3063
Fragments arranged by size:
3063 1494 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2993 4377 4645 1632
Size: 1361 1384 268 1917
Fragments arranged by size:
1917 1384 1361 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4733
AflIII A'CryG_T
Cuts at: 1203 3829 4806 1203
Size: 2626 977 1327
Fragments arranged by size:
2626 1327 977
AgeI A'CCGG_T
Cuts at: 3761 3761
Size: 4930
AhdI GACnn_n'nnGTC
Cuts at: 3659 3659
Size: 4930
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4930
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4930
AlwNI CAG_nnn'CTG
Cuts at: 2180 2673 4245 2180
Size: 493 1572 2865
Fragments arranged by size:
2865 1572 493
ApaLI G'TGCA_C
Cuts at: 2987 4143 2987
Size: 1156 3774
ApoI r'AATT_y
Cuts at: 1493 2239 2465 2617 4726 1493
Size: 746 226 152 2109 1697
Fragments arranged by size:
2109 1697 746 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3484 160
Size: 872 2452 1606
Fragments arranged by size:
2452 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4930
AvaI C'yCGr_G
Cuts at: 1870 1943 2255 1870
Size: 73 312 4545
Fragments arranged by size:
4545 312 73
BamHI G'GATC_C
Cuts at: 2260 2627 2260
Size: 367 4563
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2251 3706 618
Size: 325 217 627 464 1455 1842
Fragments arranged by size:
1842 1455 627 464 325 217
BanII G_rGCy'C
Cuts at: 1391 2249 1391
Size: 858 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3959
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3121 3155 1903
Size: 34 1184 34 3678
Fragments arranged by size:
3678 1184 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2822 4032 2822
Size: 1210 3720
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4930
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3541 136
Size: 107 122 71 631 605 1869 1525
Fragments arranged by size:
1869 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4925 4925
Size: 4930
BlpI GC'TnA_GC
Cuts at: 2347 2347
Size: 4930
Bme1580I G_kGCm'C
Cuts at: 1790 2991 4147 1790
Size: 1201 1156 2573
Fragments arranged by size:
2573 1201 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4930
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2661 3619 442
Size: 416 53 83 399 24 1244 958 1753
Fragments arranged by size:
1753 1244 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3590 1239
Size: 2351 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3055 3920 4218 3055
Size: 865 298 3767
Fragments arranged by size:
3767 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2110 3593 882
Size: 617 611 1483 2219
Fragments arranged by size:
2219 1483 617 611
BsaAI yAC'GTr
Cuts at: 493 1204 2149 2721 493
Size: 711 945 572 2702
Fragments arranged by size:
2702 945 711 572
BsaBI GATnn'nnATC
Cuts at: 4924 4924
Size: 4930
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2737 3119 3754 275
Size: 53 83 186 936 1204 382 635 1451
Fragments arranged by size:
1451 1204 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3363 3761 4035 4182 4593 3363
Size: 398 274 147 411 3700
Fragments arranged by size:
3700 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4705
Fragments arranged by size:
4705 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4930
BseYI C'CCAG_C
Cuts at: 4133 4629 4133
Size: 496 4434
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2380 3141 4169 1540
Size: 275 186 379 761 1028 2301
Fragments arranged by size:
2301 1028 761 379 275 186
BsiHKAI G_wGCw'C
Cuts at: 2991 3076 4147 2991
Size: 85 1071 3774
Fragments arranged by size:
3774 1071 85
BsmI GAATG_Cn'
Cuts at: 2441 2534 2441
Size: 93 4837
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2249 2991 3076 4147 1391
Size: 399 459 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 459 399 85
BspEI T'CCGG_A
Cuts at: 4593 4593
Size: 4930
BspHI T'CATG_A
Cuts at: 2819 4549 2819
Size: 1730 3200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2285 844
Size: 1441 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2817 1394
Size: 429 994 3507
Fragments arranged by size:
3507 994 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3425 3599 65
Size: 1183 2177 174 1396
Fragments arranged by size:
2177 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3574 3761 1700
Size: 110 1764 187 2869
Fragments arranged by size:
2869 1764 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4930
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4930
BssSI C'ACGA_G
Cuts at: 2990 4002 2990
Size: 1012 3918
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4930
BstBI TT'CG_AA
Cuts at: 3734 3734
Size: 4930
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4930
BstYI r'GATC_y
Cuts at: 1972 2158 2260 2627 3012 3029 4481 4567 4579
Size: 186 102 367 385 17 1452 86 12
Cuts at: 4579 4925 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 186 102 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2118 513
Size: 552 1053 3325
Fragments arranged by size:
3325 1053 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4191
Fragments arranged by size:
4191 683 56
BtsI GCAGTG_nn'
Cuts at: 2446 3240 3260 2446
Size: 794 20 4116
Fragments arranged by size:
4116 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4930
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4895
DraI TTT'AAA
Cuts at: 2234 2234
Size: 4930
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3937 809
Size: 507 2621 1802
Fragments arranged by size:
2621 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2377 3266 4895
Size: 54 1425 50 275 18 547 889 1629
Cuts at: 4895 8
Size: 43
Fragments arranged by size:
1629 1425 889 547 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2377 1537
Size: 275 565 4090
Fragments arranged by size:
4090 565 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2860 1353
Size: 1507 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3517 3891 4037 387
Size: 1064 138 1928 374 146 1280
Fragments arranged by size:
1928 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2993 3590 4377 4645 1239
Size: 393 1361 597 787 268 1524
Fragments arranged by size:
1524 1361 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2239 2617 2239
Size: 378 4552
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 4930
FspI TGC'GCA
Cuts at: 3436 3436
Size: 4930
HaeII r_GCGC'y
Cuts at: 1678 4077 1678
Size: 2399 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 3571 3603 3645
Size: 32 135 32 431 32 1536 32 42
Cuts at: 3645 3677 1373
Size: 32 2626
Fragments arranged by size:
2626 1536 431 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2274 2520 669
Size: 988 281 336 246 3079
Fragments arranged by size:
3079 988 336 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4930
HpaI GTT'AAC
Cuts at: 2520 2520
Size: 4930
KpnI G_GTAC'C
Cuts at: 2255 2255
Size: 4930
MfeI C'AATT_G
Cuts at: 2529 2529
Size: 4930
MluI A'CGCG_T
Cuts at: 4806 4806
Size: 4930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4044 4228 1305
Size: 2739 184 2007
Fragments arranged by size:
2739 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3108
Fragments arranged by size:
3108 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2888 3247 3406 518
Size: 648 1008 714 359 159 2042
Fragments arranged by size:
2042 1008 714 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2120 2346 3023 4171 4416 1606
Size: 514 226 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 514 245 226
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4378
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4930
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4930
NotI GC'GGCC_GC
Cuts at: 2377 2377
Size: 4930
NruI TCG'CGA
Cuts at: 2202 2202
Size: 4930
NspI r_CATG'y
Cuts at: 1132 1229 2020 2288 3833 1132
Size: 97 791 268 1545 2229
Fragments arranged by size:
2229 1545 791 268 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4930
PciI A'CATG_T
Cuts at: 3829 3829
Size: 4930
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4930
PmeI GTTT'AAAC
Cuts at: 2234 2234
Size: 4930
PmlI CAC'GTG
Cuts at: 2149 2149
Size: 4930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2195 2227 2999 3031 4512 4544 2195
Size: 32 772 32 1481 32 2581
Fragments arranged by size:
2581 1481 772 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4930
PsiI TTA'TAA
Cuts at: 2500 2500
Size: 4930
PstI C_TGCA'G
Cuts at: 830 2282 830
Size: 1452 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4930
SacII CC_GC'GG
Cuts at: 2121 2121
Size: 4930
SalI G'TCGA_C
Cuts at: 1936 2272 1936
Size: 336 4594
SbfI CC_TGCA'GG
Cuts at: 2282 2282
Size: 4930
ScaI AGT'ACT
Cuts at: 3178 4890 3178
Size: 1712 3218
SfcI C'TryA_G
Cuts at: 826 1045 2278 3413 4094 4285 826
Size: 219 1233 1135 681 191 1471
Fragments arranged by size:
1471 1233 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4930
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4930
SmaI CCC'GGG
Cuts at: 1872 2257 1872
Size: 385 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3034 3935 4197 820
Size: 197 926 1091 901 262 1553
Fragments arranged by size:
1553 1091 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2721 493
Size: 2228 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4930
SphI G_CATG'C
Cuts at: 1132 2288 1132
Size: 1156 3774
SspI AAT'ATT
Cuts at: 5 52 2854 5
Size: 47 2802 2081
Fragments arranged by size:
2802 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4930
StyI C'CwwG_G
Cuts at: 513 1065 2369 513
Size: 552 1304 3074
Fragments arranged by size:
3074 1304 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2957 2974 3127 3312 3757 325
Size: 1255 1377 17 153 185 445 1498
Fragments arranged by size:
1498 1377 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3176 4888 96
Size: 274 80 33 51 1443 1199 1712 138
Fragments arranged by size:
1712 1443 1199 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4890 4922 4890
Size: 32 4898
Tth111I GACn'n_nGTC
Cuts at: 1316 2096 1316
Size: 780 4150
XbaI T'CTAG_A
Cuts at: 2266 2266
Size: 4930
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2059
Size: 4930
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4930
XmaI C'CCGG_G
Cuts at: 1870 2255 1870
Size: 385 4545
XmnI GAAnn'nnTTC
Cuts at: 2210 3059 2210
Size: 849 4081
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2738 276
Size: 53 83 186 936 1204 2468
Fragments arranged by size:
2468 1204 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI