Restriction map for
pFN21AB3684
(Circular) (Six-base) MAPSORT of: pFN21AB3684.seq Check: 6153 from: 1 to: 6496
pFN21AB3684 6496 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2900 3480 2900
Size: 580 5916
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4306 278
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 3839 1937
Size: 1071 831 4594
Fragments arranged by size:
4594 1071 831
Acc65I G'GTAC_C
Cuts at: 3817 3817
Size: 6496
AclI AA'CG_TT
Cuts at: 1563 4623 4996 1563
Size: 3060 373 3063
Fragments arranged by size:
3063 3060 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2112 2669 3552 4559 5943 6211 1632
Size: 480 557 883 1007 1384 268 1917
Fragments arranged by size:
1917 1384 1007 883 557 480 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6299
AflIII A'CryG_T
Cuts at: 1203 2836 5395 6372 1203
Size: 1633 2559 977 1327
Fragments arranged by size:
2559 1633 1327 977
AgeI A'CCGG_T
Cuts at: 5327 5327
Size: 6496
AhdI GACnn_n'nnGTC
Cuts at: 2422 5225 2422
Size: 2803 3693
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6496
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 6464
AlwNI CAG_nnn'CTG
Cuts at: 4239 5811 4239
Size: 1572 4924
ApaI G_GGCC'C
Cuts at: 2410 3271 3770 2410
Size: 861 499 5136
Fragments arranged by size:
5136 861 499
ApaLI G'TGCA_C
Cuts at: 4553 5709 4553
Size: 1156 5340
ApoI r'AATT_y
Cuts at: 1493 3805 4031 4183 6292 1493
Size: 2312 226 152 2109 1697
Fragments arranged by size:
2312 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2271 5050 160
Size: 872 1239 2779 1606
Fragments arranged by size:
2779 1606 1239 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
AvaI C'yCGr_G
Cuts at: 1870 1943 3821 1870
Size: 73 1878 4545
Fragments arranged by size:
4545 1878 73
BamHI G'GATC_C
Cuts at: 3826 4193 3826
Size: 367 6129
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2349 2618 3520 3817 5272
Size: 325 217 627 562 269 902 297 1455
Cuts at: 5272 618
Size: 1842
Fragments arranged by size:
1842 1455 902 627 562 325 297 269
217
BanII G_rGCy'C
Cuts at: 1391 2219 2410 3210 3271 3462 3770 3815 1391
Size: 828 191 800 61 191 308 45 4072
Fragments arranged by size:
4072 828 800 308 191 191 61 45
BbeI G_GCGC'C
Cuts at: 2353 2353
Size: 6496
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3237 928
Size: 971 1338 4187
Fragments arranged by size:
4187 1338 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4687 4721 1903
Size: 34 2750 34 3678
Fragments arranged by size:
3678 2750 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4388 5598 4388
Size: 1210 5286
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6496
BfrBI ATG'CAT
Cuts at: 3652 3652
Size: 6496
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2528 3198 5107
Size: 107 122 71 631 605 856 670 1909
Cuts at: 5107 136
Size: 1525
Fragments arranged by size:
1909 1525 856 670 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3513 6491 3513
Size: 2978 3518
BlpI GC'TnA_GC
Cuts at: 3913 3913
Size: 6496
Bme1580I G_kGCm'C
Cuts at: 1790 2410 2506 2776 3271 3378 3523 3770 4557
Size: 620 96 270 495 107 145 247 787
Cuts at: 4557 5713 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 787 620 495 270 247 145
107 96
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2166 2428 3265
Size: 416 53 83 399 24 749 262 837
Cuts at: 3265 4227 5185 442
Size: 962 958 1753
Fragments arranged by size:
1753 962 958 837 749 416 399 262
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6496
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 6496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2106 5156 1239
Size: 867 3050 2579
Fragments arranged by size:
3050 2579 867
Bpu10I CC'TnA_GC
Cuts at: 2218 2218
Size: 6496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2591 3107 3476 4621 5486 5784 2591
Size: 516 369 1145 865 298 3303
Fragments arranged by size:
3303 1145 865 516 369 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5159 882
Size: 617 3660 2219
Fragments arranged by size:
3660 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4287 493
Size: 711 3083 2702
Fragments arranged by size:
3083 2702 711
BsaBI GATnn'nnATC
Cuts at: 2280 6490 2280
Size: 4210 2286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2350 3321 3670 4303
Size: 53 83 186 936 817 971 349 633
Cuts at: 4303 4685 5320 275
Size: 382 635 1451
Fragments arranged by size:
1451 971 936 817 635 633 382 349
186 83 53
BsaWI w'CCGG_w
Cuts at: 4929 5327 5601 5748 6159 4929
Size: 398 274 147 411 5266
Fragments arranged by size:
5266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3429 3459 1178
Size: 30 165 30 2026 30 4215
Fragments arranged by size:
4215 2026 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2330 2342 3241 3730 3773 3776 1186
Size: 904 240 12 899 489 43 3 3906
Fragments arranged by size:
3906 904 899 489 240 43 12 3
BseYI C'CCAG_C
Cuts at: 2491 2844 2956 3734 5699 6195 2491
Size: 353 112 778 1965 496 2792
Fragments arranged by size:
2792 1965 778 496 353 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2564 2585 3800 1163
Size: 573 828 21 1215 3859
Fragments arranged by size:
3859 1215 828 573 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3946 4707 5735 1540
Size: 275 186 1945 761 1028 2301
Fragments arranged by size:
2301 1945 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2219 3210 4557 4642 5713 2219
Size: 991 1347 85 1071 3002
Fragments arranged by size:
3002 1347 1071 991 85
BsmI GAATG_Cn'
Cuts at: 4007 4100 4007
Size: 93 6403
BsmBI CGTCTCn'nnnn_
Cuts at: 3661 3661
Size: 6496
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2410 2506 2776 3210 3271 3378
Size: 399 429 191 96 270 434 61 107
Cuts at: 3378 3462 3523 3770 3815 4557 4642 5713 1391
Size: 84 61 247 45 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 434 429 399 270 247
191 107 96 85 84 61 61 45
BspEI T'CCGG_A
Cuts at: 6159 6159
Size: 6496
BspHI T'CATG_A
Cuts at: 4385 6115 4385
Size: 1730 4766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2776 2900 3480 3851 844
Size: 1932 124 580 371 3489
Fragments arranged by size:
3489 1932 580 371 124
BsrBI CCG'CTC
Cuts at: 1394 1823 2298 2404 2485 3360 3589 4383 1394
Size: 429 475 106 81 875 229 794 3507
Fragments arranged by size:
3507 875 794 475 429 229 106 81
BsrDI GCAATG_nn'
Cuts at: 65 1248 4991 5165 65
Size: 1183 3743 174 1396
Fragments arranged by size:
3743 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3678 5140 5327 1700
Size: 110 1868 1462 187 2869
Fragments arranged by size:
2869 1868 1462 187 110
BsrGI T'GTAC_A
Cuts at: 96 2356 3186 96
Size: 2260 830 3406
Fragments arranged by size:
3406 2260 830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6496
BssSI C'ACGA_G
Cuts at: 4556 5568 4556
Size: 1012 5484
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3787 794
Size: 2993 3503
BstBI TT'CG_AA
Cuts at: 5300 5300
Size: 6496
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6496
BstYI r'GATC_y
Cuts at: 1972 3513 3826 4193 4578 4595 6047 6133 6145
Size: 1541 313 367 385 17 1452 86 12
Cuts at: 6145 6491 1972
Size: 346 1977
Fragments arranged by size:
1977 1541 1452 385 367 346 313 86
17 12
BstZ17I GTA'TAC
Cuts at: 3009 3009
Size: 6496
Bsu36I CC'TnA_GG
Cuts at: 2113 2113
Size: 6496
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5944
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2655 487
Size: 683 56 1429 4328
Fragments arranged by size:
4328 1429 683 56
BtsI GCAGTG_nn'
Cuts at: 3054 3788 4012 4806 4826 3054
Size: 734 224 794 20 4724
Fragments arranged by size:
4724 794 734 224 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2820 2855 529
Size: 35 2256 35 4170
Fragments arranged by size:
4170 2256 35 35
DraI TTT'AAA
Cuts at: 3800 3800
Size: 6496
DraIII CAC_nnn'GTG
Cuts at: 2817 3148 2817
Size: 331 6165
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5503 809
Size: 507 4187 1802
Fragments arranged by size:
4187 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2628 2704 2763
Size: 54 1425 50 275 18 798 76 59
Cuts at: 2763 3213 3943 4832 6461 8
Size: 450 730 889 1629 43
Fragments arranged by size:
1629 1425 889 798 730 450 275 76
59 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3943 1537
Size: 275 2131 4090
Fragments arranged by size:
4090 2131 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 2301 2307 2319 2331 3248 4426 1353
Size: 735 213 6 12 12 917 1178 3423
Fragments arranged by size:
3423 1178 917 735 213 12 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2543 5083 5457 5603 387
Size: 1064 138 954 2540 374 146 1280
Fragments arranged by size:
2540 1280 1064 954 374 146 138
EcoICRI GAG'CTC
Cuts at: 2217 3208 2217
Size: 991 5505
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2112 2669 3552 4559 5156 5943
Size: 393 474 6 557 883 1007 597 787
Cuts at: 5943 6211 1239
Size: 268 1524
Fragments arranged by size:
1524 1007 883 787 597 557 474 393
268 6
EcoO109I rG'GnC_Cy
Cuts at: 2407 3267 3766 3767 2407
Size: 860 499 1 5136
Fragments arranged by size:
5136 860 499 1
EcoRI G'AATT_C
Cuts at: 3805 4183 3805
Size: 378 6118
FspI TGC'GCA
Cuts at: 5002 5002
Size: 6496
HaeII r_GCGC'y
Cuts at: 1678 2353 3685 5643 1678
Size: 675 1332 1958 2531
Fragments arranged by size:
2531 1958 1332 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 2219 2251 2372
Size: 32 135 32 494 32 121 32 121
Cuts at: 2372 2404 2408 2440 3383 3415 3658 3690 3704
Size: 32 4 32 943 32 243 32 14
Cuts at: 3704 3736 5137 5169 5211 5243 1373
Size: 32 1401 32 42 32 2626
Fragments arranged by size:
2626 1401 943 494 243 135 121 121
42 32 32 32 32 32 32 32
32 32 32 32 14 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3840 4086 669
Size: 988 281 1902 246 3079
Fragments arranged by size:
3079 1902 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3534 748
Size: 2786 3710
HpaI GTT'AAC
Cuts at: 4086 4086
Size: 6496
KasI G'GCGC_C
Cuts at: 2349 2349
Size: 6496
KpnI G_GTAC'C
Cuts at: 3821 3821
Size: 6496
MfeI C'AATT_G
Cuts at: 4095 4095
Size: 6496
MluI A'CGCG_T
Cuts at: 6372 6372
Size: 6496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5610 5794 1305
Size: 4305 184 2007
Fragments arranged by size:
4305 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2630 2706 2765 3215 10
Size: 54 1425 343 798 76 59 450 3291
Fragments arranged by size:
3291 1425 798 450 343 76 59 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 3261 4454 4813 4972 518
Size: 648 957 1138 1193 359 159 2042
Fragments arranged by size:
2042 1193 1138 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3530 3912 4589 5737 5982 1606
Size: 1924 382 677 1148 245 2120
Fragments arranged by size:
2120 1924 1148 677 382 245
NarI GG'CG_CC
Cuts at: 2350 2350
Size: 6496
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5944
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6496
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6496
NotI GC'GGCC_GC
Cuts at: 3943 3943
Size: 6496
NsiI A_TGCA'T
Cuts at: 3654 3654
Size: 6496
NspI r_CATG'y
Cuts at: 1132 1229 3637 3790 3854 5399 1132
Size: 97 2408 153 64 1545 2229
Fragments arranged by size:
2408 2229 1545 153 97 64
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6496
PciI A'CATG_T
Cuts at: 5395 5395
Size: 6496
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2869 1108
Size: 1761 4735
PmeI GTTT'AAAC
Cuts at: 3800 3800
Size: 6496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4565 4597 6078 6110 4565
Size: 32 1481 32 4951
Fragments arranged by size:
4951 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2256 1613
Size: 643 5853
PsiI TTA'TAA
Cuts at: 4066 4066
Size: 6496
PspOMI G'GGCC_C
Cuts at: 2406 3267 3766 2406
Size: 861 499 5136
Fragments arranged by size:
5136 861 499
PstI C_TGCA'G
Cuts at: 830 2562 2911 3848 830
Size: 1732 349 937 3478
Fragments arranged by size:
3478 1732 937 349
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6496
PvuII CAG'CTG
Cuts at: 3530 3530
Size: 6496
SacI G_AGCT'C
Cuts at: 2219 3210 2219
Size: 991 5505
SalI G'TCGA_C
Cuts at: 1936 3838 1936
Size: 1902 4594
SapI GCTCTTCn'nnn_
Cuts at: 2331 2331
Size: 6496
SbfI CC_TGCA'GG
Cuts at: 3848 3848
Size: 6496
ScaI AGT'ACT
Cuts at: 2688 3120 4744 6456 2688
Size: 432 1624 1712 2728
Fragments arranged by size:
2728 1712 1624 432
SexAI A'CCwGG_T
Cuts at: 3144 3144
Size: 6496
SfcI C'TryA_G
Cuts at: 826 1045 2558 2907 3844 4979 5660 5851 826
Size: 219 1513 349 937 1135 681 191 1471
Fragments arranged by size:
1513 1471 1135 937 681 349 219 191
SfoI GGC'GCC
Cuts at: 2351 2351
Size: 6496
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
SgrAI Cr'CCGG_yG
Cuts at: 1700 3678 1700
Size: 1978 4518
SmaI CCC'GGG
Cuts at: 1872 3823 1872
Size: 1951 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2570 3122 3455 4600 5501 5763
Size: 197 926 627 552 333 1145 901 262
Cuts at: 5763 820
Size: 1553
Fragments arranged by size:
1553 1145 926 901 627 552 333 262
197
SnaBI TAC'GTA
Cuts at: 493 4287 493
Size: 3794 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6496
SphI G_CATG'C
Cuts at: 1132 3790 3854 1132
Size: 2658 64 3774
Fragments arranged by size:
3774 2658 64
SspI AAT'ATT
Cuts at: 5 52 4420 5
Size: 47 4368 2081
Fragments arranged by size:
4368 2081 47
StuI AGG'CCT
Cuts at: 1517 2132 2522 1517
Size: 615 390 5491
Fragments arranged by size:
5491 615 390
StyI C'CwwG_G
Cuts at: 513 1065 3935 513
Size: 552 2870 3074
Fragments arranged by size:
3074 2870 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2410 3041 3538 4523 4540 4693 4878
Size: 1255 830 631 497 985 17 153 185
Cuts at: 4878 5323 325
Size: 445 1498
Fragments arranged by size:
1498 1255 985 830 631 497 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2356 2686 3118
Size: 274 80 33 51 1443 379 330 432
Cuts at: 3118 3186 4742 6454 96
Size: 68 1556 1712 138
Fragments arranged by size:
1712 1556 1443 432 379 330 274 138
80 68 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6456 6488 6456
Size: 32 6464
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6496
XbaI T'CTAG_A
Cuts at: 3832 3832
Size: 6496
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6496
XmaI C'CCGG_G
Cuts at: 1870 3821 1870
Size: 1951 4545
XmnI GAAnn'nnTTC
Cuts at: 4625 4625
Size: 6496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4304 276
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BsiWI BstXI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SfiI SrfI SwaI XcmI