Restriction map for
pFN21AB3683
(Circular) (Six-base) MAPSORT of: pFN21AB3683.seq Check: 9337 from: 1 to: 8227
pFN21AB3683 8227 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3456 3456
Size: 8227
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 6037 278
Size: 53 83 186 936 2245 2256 2468
Fragments arranged by size:
2468 2256 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 3871 4407 4816 5570 1937
Size: 1497 437 536 409 754 4594
Fragments arranged by size:
4594 1497 754 536 437 409
Acc65I G'GTAC_C
Cuts at: 5548 5548
Size: 8227
AclI AA'CG_TT
Cuts at: 1563 6354 6727 1563
Size: 4791 373 3063
Fragments arranged by size:
4791 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 2282 2408 3822 4405 6290 7674 7942
Size: 600 50 126 1414 583 1885 1384 268
Cuts at: 7942 1632
Size: 1917
Fragments arranged by size:
1917 1885 1414 1384 600 583 268 126
50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8030
AflIII A'CryG_T
Cuts at: 1203 4502 7126 8103 1203
Size: 3299 2624 977 1327
Fragments arranged by size:
3299 2624 1327 977
AgeI A'CCGG_T
Cuts at: 7058 7058
Size: 8227
AhdI GACnn_n'nnGTC
Cuts at: 4938 6956 4938
Size: 2018 6209
AleI CACnn'nnGTG
Cuts at: 1166 5187 1166
Size: 4021 4206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3093 3125 3093
Size: 32 8195
AlwNI CAG_nnn'CTG
Cuts at: 5421 5970 7542 5421
Size: 549 1572 6106
Fragments arranged by size:
6106 1572 549
ApaLI G'TGCA_C
Cuts at: 6284 7440 6284
Size: 1156 7071
ApoI r'AATT_y
Cuts at: 1493 2274 2457 2683 2959 3232 3266 3460 3577
Size: 781 183 226 276 273 34 194 117
Cuts at: 3577 3632 3658 3669 4754 4805 5536 5762 5914
Size: 55 26 11 1085 51 731 226 152
Cuts at: 5914 8023 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1085 781 731 276 273 226
226 194 183 152 117 55 51 34
26 11
AseI AT'TA_AT
Cuts at: 160 1032 2565 6781 160
Size: 872 1533 4216 1606
Fragments arranged by size:
4216 1606 1533 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8227
AvaI C'yCGr_G
Cuts at: 1870 1943 5552 1870
Size: 73 3609 4545
Fragments arranged by size:
4545 3609 73
BamHI G'GATC_C
Cuts at: 2542 5557 5924 2542
Size: 3015 367 4845
Fragments arranged by size:
4845 3015 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2741 5548 7003 618
Size: 325 217 627 954 2807 1455 1842
Fragments arranged by size:
2807 1842 1455 954 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5546 1391
Size: 4155 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2190 4383 928
Size: 971 291 2193 4772
Fragments arranged by size:
4772 2193 971 291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6418 6452 1903
Size: 34 4481 34 3678
Fragments arranged by size:
4481 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3095 6119 7329 3095
Size: 3024 1210 3993
Fragments arranged by size:
3993 3024 1210
BclI T'GATC_A
Cuts at: 1548 3367 3813 4147 4474 1548
Size: 1819 446 334 327 5301
Fragments arranged by size:
5301 1819 446 334 327
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3590 6838 136
Size: 107 122 71 631 605 1918 3248 1525
Fragments arranged by size:
3248 1918 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5237 8222 5237
Size: 2985 5242
BlpI GC'TnA_GC
Cuts at: 2217 5644 2217
Size: 3427 4800
Bme1580I G_kGCm'C
Cuts at: 1790 6288 7444 1790
Size: 4498 1156 2573
Fragments arranged by size:
4498 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8227
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3370 5299 5958
Size: 416 53 83 399 24 1953 1929 659
Cuts at: 5958 6916 442
Size: 958 1753
Fragments arranged by size:
1953 1929 1753 958 659 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3491 1056
Size: 2435 5792
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3417 4023 4554 6887 1239
Size: 2178 606 531 2333 2579
Fragments arranged by size:
2579 2333 2178 606 531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2699 6352 7217 7515 2699
Size: 3653 865 298 3411
Fragments arranged by size:
3653 3411 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3071 3536 6890 882
Size: 617 1572 465 3354 2219
Fragments arranged by size:
3354 2219 1572 617 465
BsaAI yAC'GTr
Cuts at: 493 1204 2361 4505 6018 493
Size: 711 1157 2144 1513 2702
Fragments arranged by size:
2702 2144 1513 1157 711
BsaBI GATnn'nnATC
Cuts at: 2090 2511 3788 3842 5343 8221 2090
Size: 421 1277 54 1501 2878 2096
Fragments arranged by size:
2878 2096 1501 1277 421 54
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3778 6034 6416 7051
Size: 53 83 186 936 2245 2256 382 635
Cuts at: 7051 275
Size: 1451
Fragments arranged by size:
2256 2245 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6660 7058 7332 7479 7890 6660
Size: 398 274 147 411 6997
Fragments arranged by size:
6997 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4581 4611 1178
Size: 30 165 30 3178 30 4794
Fragments arranged by size:
4794 3178 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2181 2745 2991 1186
Size: 995 564 246 6422
Fragments arranged by size:
6422 995 564 246
BseYI C'CCAG_C
Cuts at: 3191 3720 7430 7926 3191
Size: 529 3710 496 3492
Fragments arranged by size:
3710 3492 529 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5048 1163
Size: 573 3312 4342
Fragments arranged by size:
4342 3312 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5677 6438 7466 1540
Size: 275 186 3676 761 1028 2301
Fragments arranged by size:
3676 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2317 4652 6288 6373 7444 2317
Size: 2335 1636 85 1071 3100
Fragments arranged by size:
3100 2335 1636 1071 85
BsmI GAATG_Cn'
Cuts at: 2537 3206 4958 5417 5460 5738 5831 2537
Size: 669 1752 459 43 278 93 4933
Fragments arranged by size:
4933 1752 669 459 278 93 43
BsmBI CGTCTCn'nnnn_
Cuts at: 3471 3769 4697 3471
Size: 298 928 7001
Fragments arranged by size:
7001 928 298
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2317 4652 5546 6288 6373 7444 1391
Size: 399 527 2335 894 742 85 1071 2174
Fragments arranged by size:
2335 2174 1071 894 742 527 399 85
BspEI T'CCGG_A
Cuts at: 7890 7890
Size: 8227
BspHI T'CATG_A
Cuts at: 4389 6116 7846 4389
Size: 1727 1730 4770
Fragments arranged by size:
4770 1730 1727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3456 5582 844
Size: 2612 2126 3489
Fragments arranged by size:
3489 2612 2126
BsrBI CCG'CTC
Cuts at: 1394 1823 6114 1394
Size: 429 4291 3507
Fragments arranged by size:
4291 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4038 4347 6722 6896 65
Size: 1183 2790 309 2375 174 1396
Fragments arranged by size:
2790 2375 1396 1183 309 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6871 7058 1700
Size: 110 5061 187 2869
Fragments arranged by size:
5061 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5088 96
Size: 4992 3235
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8227
BssSI C'ACGA_G
Cuts at: 5187 5241 6287 7299 5187
Size: 54 1046 1012 6115
Fragments arranged by size:
6115 1046 1012 54
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3686 5421 794
Size: 2892 1735 3600
Fragments arranged by size:
3600 2892 1735
BstBI TT'CG_AA
Cuts at: 7031 7031
Size: 8227
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8227
BstYI r'GATC_y
Cuts at: 1972 2542 2884 3184 3252 3608 3739 3843 3967
Size: 570 342 300 68 356 131 104 124
Cuts at: 3967 5208 5237 5338 5557 5924 6309 6326 7778
Size: 1241 29 101 219 367 385 17 1452
Cuts at: 7778 7864 7876 8222 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1241 570 385 367 356 346
342 300 219 131 124 104 101 86
68 29 17 12
Bsu36I CC'TnA_GG
Cuts at: 3764 3764
Size: 8227
BtgI C'CryG_G
Cuts at: 513 1065 4411 5226 5293 513
Size: 552 3346 815 67 3447
Fragments arranged by size:
3447 3346 815 552 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7488
Fragments arranged by size:
7488 683 56
BtsI GCAGTG_nn'
Cuts at: 5743 6537 6557 5743
Size: 794 20 7413
Fragments arranged by size:
7413 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8227
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2238 2273 5350 5385 529
Size: 35 1674 35 3077 35 3371
Fragments arranged by size:
3371 3077 1674 35 35 35
DraI TTT'AAA
Cuts at: 3459 5531 3459
Size: 2072 6155
DraIII CAC_nnn'GTG
Cuts at: 4091 4091
Size: 8227
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3430 4288 7234 809
Size: 507 2114 858 2946 1802
Fragments arranged by size:
2946 2114 1802 858 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3975 4344 5674
Size: 54 1425 50 275 18 2145 369 1330
Cuts at: 5674 6563 8192 8
Size: 889 1629 43
Fragments arranged by size:
2145 1629 1425 1330 889 369 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5674 1537
Size: 275 3862 4090
Fragments arranged by size:
4090 3862 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2744 3136 3908 6157 1353
Size: 1391 392 772 2249 3423
Fragments arranged by size:
3423 2249 1391 772 392
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6814 7188 7334 387
Size: 1064 138 5225 374 146 1280
Fragments arranged by size:
5225 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2282 2408 3417 3822 4023 4405
Size: 393 600 50 126 1009 405 201 382
Cuts at: 4405 4554 6290 6887 7674 7942 1239
Size: 149 1736 597 787 268 1524
Fragments arranged by size:
1736 1524 1009 787 600 597 405 393
382 268 201 149 126 50
EcoO109I rG'GnC_Cy
Cuts at: 5083 5198 5397 5083
Size: 115 199 7913
Fragments arranged by size:
7913 199 115
EcoRI G'AATT_C
Cuts at: 2683 5536 5914 2683
Size: 2853 378 4996
Fragments arranged by size:
4996 2853 378
EcoRV GAT'ATC
Cuts at: 4897 4897
Size: 8227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2438 2438
Size: 8227
FspI TGC'GCA
Cuts at: 6733 6733
Size: 8227
HaeII r_GCGC'y
Cuts at: 1678 7374 1678
Size: 5696 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2419 2451 2654 2686 3169
Size: 32 135 32 847 32 203 32 483
Cuts at: 3169 3201 4197 4229 4924 4956 5026 5058 5170
Size: 32 996 32 695 32 70 32 112
Cuts at: 5170 5202 5222 5254 6868 6900 6942 6974 1373
Size: 32 20 32 1614 32 42 32 2626
Fragments arranged by size:
2626 1614 996 847 695 483 203 135
112 70 42 32 32 32 32 32
32 32 32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 2478 4817 5023 5571 5817 669
Size: 988 281 540 2339 206 548 246 3079
Fragments arranged by size:
3079 2339 988 548 540 281 246 206
HindIII A'AGCT_T
Cuts at: 748 2925 748
Size: 2177 6050
HpaI GTT'AAC
Cuts at: 5023 5817 5023
Size: 794 7433
KpnI G_GTAC'C
Cuts at: 5552 5552
Size: 8227
MfeI C'AATT_G
Cuts at: 3929 4338 5826 3929
Size: 409 1488 6330
Fragments arranged by size:
6330 1488 409
MluI A'CGCG_T
Cuts at: 8103 8103
Size: 8227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2868 3586 3706 7341 7525 1305
Size: 1563 718 120 3635 184 2007
Fragments arranged by size:
3635 2007 1563 718 184 120
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3977 4346 10
Size: 54 1425 343 2145 369 3891
Fragments arranged by size:
3891 2145 1425 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2226 2816 3057 3794 5187 5406 5410
Size: 648 1060 590 241 737 1393 219 4
Cuts at: 5410 6185 6544 6703 518
Size: 775 359 159 2042
Fragments arranged by size:
2042 1393 1060 775 737 648 590 359
241 219 159 4
MspA1I CmG'CkG
Cuts at: 1606 2134 3199 4403 4413 5643 6320 7468 7713
Size: 528 1065 1204 10 1230 677 1148 245
Cuts at: 7713 1606
Size: 2120
Fragments arranged by size:
2120 1230 1204 1148 1065 677 528 245
10
NcoI C'CATG_G
Cuts at: 513 1065 5226 5293 513
Size: 552 4161 67 3447
Fragments arranged by size:
4161 3447 552 67
NdeI CA'TA_TG
Cuts at: 387 5163 5407 387
Size: 4776 244 3207
Fragments arranged by size:
4776 3207 244
NheI G'CTAG_C
Cuts at: 1052 3487 1052
Size: 2435 5792
NotI GC'GGCC_GC
Cuts at: 5674 5674
Size: 8227
NspI r_CATG'y
Cuts at: 1132 1229 2082 2225 2722 4515 4532 5469 5585
Size: 97 853 143 497 1793 17 937 116
Cuts at: 5585 7130 1132
Size: 1545 2229
Fragments arranged by size:
2229 1793 1545 937 853 497 143 116
97 17
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8227
PciI A'CATG_T
Cuts at: 7126 7126
Size: 8227
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4796 1108
Size: 3688 4539
PmeI GTTT'AAAC
Cuts at: 5531 5531
Size: 8227
PmlI CAC'GTG
Cuts at: 4505 4505
Size: 8227
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6296 6328 7809 7841 6296
Size: 32 1481 32 6682
Fragments arranged by size:
6682 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5083 5198 5397 5083
Size: 115 199 7913
Fragments arranged by size:
7913 199 115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8227
PsiI TTA'TAA
Cuts at: 5797 5797
Size: 8227
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5081 5113 5081
Size: 32 8195
PstI C_TGCA'G
Cuts at: 830 3682 5579 830
Size: 2852 1897 3478
Fragments arranged by size:
3478 2852 1897
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8227
PvuII CAG'CTG
Cuts at: 2134 4403 2134
Size: 2269 5958
SacII CC_GC'GG
Cuts at: 4414 4414
Size: 8227
SalI G'TCGA_C
Cuts at: 1936 4815 5569 1936
Size: 2879 754 4594
Fragments arranged by size:
4594 2879 754
SbfI CC_TGCA'GG
Cuts at: 5579 5579
Size: 8227
ScaI AGT'ACT
Cuts at: 2704 6475 8187 2704
Size: 3771 1712 2744
Fragments arranged by size:
3771 2744 1712
SfcI C'TryA_G
Cuts at: 826 1045 2196 2204 3561 3678 3955 4404 4678
Size: 219 1151 8 1357 117 277 449 274
Cuts at: 4678 5575 6710 7391 7582 826
Size: 897 1135 681 191 1471
Fragments arranged by size:
1471 1357 1151 1135 897 681 449 277
274 219 191 117 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8227
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8227
SmaI CCC'GGG
Cuts at: 1872 5554 1872
Size: 3682 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2678 6331 7232 7494 820
Size: 197 926 735 3653 901 262 1553
Fragments arranged by size:
3653 1553 926 901 735 262 197
SnaBI TAC'GTA
Cuts at: 493 6018 493
Size: 5525 2702
SpeI A'CTAG_T
Cuts at: 152 4697 152
Size: 4545 3682
SphI G_CATG'C
Cuts at: 1132 2082 2225 2722 4532 5469 5585 1132
Size: 950 143 497 1810 937 116 3774
Fragments arranged by size:
3774 1810 950 937 497 143 116
SspI AAT'ATT
Cuts at: 5 52 5127 6151 5
Size: 47 5075 1024 2081
Fragments arranged by size:
5075 2081 1024 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8227
StyI C'CwwG_G
Cuts at: 513 1065 4114 5226 5293 5666 513
Size: 552 3049 1112 67 373 3074
Fragments arranged by size:
3074 3049 1112 552 373 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6254 6271 6424 6609 7054 325
Size: 1255 4674 17 153 185 445 1498
Fragments arranged by size:
4674 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2702 5088 6473
Size: 274 80 33 51 1443 725 2386 1385
Cuts at: 6473 8185 96
Size: 1712 138
Fragments arranged by size:
2386 1712 1443 1385 725 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8187 8219 8187
Size: 32 8195
Tth111I GACn'n_nGTC
Cuts at: 1316 4821 1316
Size: 3505 4722
XbaI T'CTAG_A
Cuts at: 5563 5563
Size: 8227
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3985 3985
Size: 8227
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8227
XmaI C'CCGG_G
Cuts at: 1870 5552 1870
Size: 3682 4545
XmnI GAAnn'nnTTC
Cuts at: 2421 2580 4956 6356 2421
Size: 159 2376 1400 4292
Fragments arranged by size:
4292 2376 1400 159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 6035 276
Size: 53 83 186 936 2245 2256 2468
Fragments arranged by size:
2468 2256 2245 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspOMI PspXI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI