Restriction map for
pFN21AB3681
(Circular) (Six-base) MAPSORT of: pFN21AB3681.seq Check: 1800 from: 1 to: 7390
pFN21AB3681 7390 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5200 278
Size: 53 83 186 936 3664 2468
Fragments arranged by size:
3664 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4733 1937
Size: 2796 4594
Acc65I G'GTAC_C
Cuts at: 4711 4711
Size: 7390
AclI AA'CG_TT
Cuts at: 1563 5517 5890 1563
Size: 3954 373 3063
Fragments arranged by size:
3954 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2477 3542 3893 4492 5453 6837 7105 1632
Size: 845 1065 351 599 961 1384 268 1917
Fragments arranged by size:
1917 1384 1065 961 845 599 351 268
AfeI AGC'GCT
Cuts at: 3800 3800
Size: 7390
AflII C'TTAA_G
Cuts at: 820 1017 3599 820
Size: 197 2582 4611
Fragments arranged by size:
4611 2582 197
AflIII A'CryG_T
Cuts at: 1203 4575 6289 7266 1203
Size: 3372 1714 977 1327
Fragments arranged by size:
3372 1714 1327 977
AgeI A'CCGG_T
Cuts at: 4627 6221 4627
Size: 1594 5796
AhdI GACnn_n'nnGTC
Cuts at: 6119 6119
Size: 7390
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3498 4304 1184
Size: 2314 806 4270
Fragments arranged by size:
4270 2314 806
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 3220 3221 3252 3253 3182
Size: 32 6 1 31 1 7319
Fragments arranged by size:
7319 32 31 6 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2699 2934 3889 4259 5133 6705 2699
Size: 235 955 370 874 1572 3384
Fragments arranged by size:
3384 1572 955 874 370 235
ApaI G_GGCC'C
Cuts at: 2269 2474 2820 2919 2946 4114 4593 2269
Size: 205 346 99 27 1168 479 5066
Fragments arranged by size:
5066 1168 479 346 205 99 27
ApaLI G'TGCA_C
Cuts at: 4150 5447 6603 4150
Size: 1297 1156 4937
Fragments arranged by size:
4937 1297 1156
ApoI r'AATT_y
Cuts at: 1493 4699 4925 5077 7186 1493
Size: 3206 226 152 2109 1697
Fragments arranged by size:
3206 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5944 160
Size: 872 4912 1606
Fragments arranged by size:
4912 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2580 2774 2820 3806 4439 4585
Size: 73 80 557 194 46 986 633 146
Cuts at: 4585 4677 4715 1870
Size: 92 38 4545
Fragments arranged by size:
4545 986 633 557 194 146 92 80
73 46 38
BamHI G'GATC_C
Cuts at: 2173 2331 4720 5087 2173
Size: 158 2389 367 4476
Fragments arranged by size:
4476 2389 367 158
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2075 2464 2485 2836
Size: 325 217 627 243 45 389 21 351
Cuts at: 2836 3976 4056 4180 4624 4711 6166 618
Size: 1140 80 124 444 87 1455 1842
Fragments arranged by size:
1842 1455 1140 627 444 389 351 325
243 217 124 87 80 45 21
BanII G_rGCy'C
Cuts at: 1391 2023 2049 2147 2269 2297 2474 2541 2820
Size: 632 26 98 122 28 177 67 279
Cuts at: 2820 2919 2946 3147 3579 3755 3813 4032 4114
Size: 99 27 201 432 176 58 219 82
Cuts at: 4114 4381 4593 4709 1391
Size: 267 212 116 4072
Fragments arranged by size:
4072 632 432 279 267 219 212 201
177 176 122 116 99 98 82 67
58 28 27 26
BbeI G_GCGC'C
Cuts at: 2034 2079 2034
Size: 45 7345
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3939 4465 928
Size: 971 2040 526 3853
Fragments arranged by size:
3853 2040 971 526
BbvCI CC'TCA_GC
Cuts at: 4496 4496
Size: 7390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3459 3493 5581 5615 1903
Size: 34 50 34 1438 34 2088 34 3678
Fragments arranged by size:
3678 2088 1438 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2647 5282 6492 2647
Size: 2635 1210 3545
Fragments arranged by size:
3545 2635 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7390
BfrBI ATG'CAT
Cuts at: 2008 3511 2008
Size: 1503 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4256 4563 6001
Size: 107 122 71 631 605 2584 307 1438
Cuts at: 6001 136
Size: 1525
Fragments arranged by size:
2584 1525 1438 631 605 307 122 107
71
BglII A'GATC_T
Cuts at: 7385 7385
Size: 7390
BlpI GC'TnA_GC
Cuts at: 4807 4807
Size: 7390
Bme1580I G_kGCm'C
Cuts at: 1790 2269 2467 2474 2490 2766 2820 2841 2919
Size: 479 198 7 16 276 54 21 78
Cuts at: 2919 2946 3078 3208 3678 3718 3979 4114 4154
Size: 27 132 130 470 40 261 135 40
Cuts at: 4154 4593 5451 6607 1790
Size: 439 858 1156 2573
Fragments arranged by size:
2573 1156 858 479 470 439 276 261
198 135 132 130 78 54 40 40
27 21 16 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7390
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2173 2241 2616
Size: 416 53 83 399 24 756 68 375
Cuts at: 2616 3065 3853 5121 6079 442
Size: 449 788 1268 958 1753
Fragments arranged by size:
1753 1268 958 788 756 449 416 399
375 83 68 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2130 2202 2309 2555 3041 3368 3470 3797
Size: 891 72 107 246 486 327 102 327
Cuts at: 3797 3911 4259 6050 1239
Size: 114 348 1791 2579
Fragments arranged by size:
2579 1791 891 486 348 327 327 246
114 107 102 72
Bpu10I CC'TnA_GC
Cuts at: 2146 4496 2146
Size: 2350 5040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3371 3612 5515 6380 6678 3371
Size: 241 1903 865 298 4083
Fragments arranged by size:
4083 1903 865 298 241
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2885 6053 882
Size: 617 1386 3168 2219
Fragments arranged by size:
3168 2219 1386 617
BsaAI yAC'GTr
Cuts at: 493 1204 5181 493
Size: 711 3977 2702
Fragments arranged by size:
3977 2702 711
BsaBI GATnn'nnATC
Cuts at: 7384 7384
Size: 7390
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2076 5197 5579
Size: 53 83 186 936 498 45 3121 382
Cuts at: 5579 6214 275
Size: 635 1451
Fragments arranged by size:
3121 1451 936 635 498 382 186 83
53 45
BsaWI w'CCGG_w
Cuts at: 2328 4627 5823 6221 6495 6642 7053 2328
Size: 2299 1196 398 274 147 411 2665
Fragments arranged by size:
2665 2299 1196 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3266 3296 4121 4151 1178
Size: 30 165 30 1863 30 825 30 4417
Fragments arranged by size:
4417 1863 825 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2903 3038 3041 3053 3056 3059 3563 3842
Size: 1717 135 3 12 3 3 504 279
Cuts at: 3842 3845 4634 1186
Size: 3 789 3942
Fragments arranged by size:
3942 1717 789 504 279 135 12 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2394 2498 2705 2766 2921 4218 4398 6593 7089
Size: 104 207 61 155 1297 180 2195 496
Cuts at: 7089 2394
Size: 2695
Fragments arranged by size:
2695 2195 1297 496 207 180 155 104
61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 3134 3320 3377 4274 1163
Size: 573 564 834 186 57 897 4279
Fragments arranged by size:
4279 897 834 573 564 186 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3249 3658 4840 5601 6629 1540
Size: 275 186 1248 409 1182 761 1028 2301
Fragments arranged by size:
2301 1248 1182 1028 761 409 275 186
BsiHKAI G_wGCw'C
Cuts at: 3147 3477 3579 3755 3813 4100 4154 5451 5536
Size: 330 102 176 58 287 54 1297 85
Cuts at: 5536 6607 3147
Size: 1071 3930
Fragments arranged by size:
3930 1297 1071 330 287 176 102 85
58 54
BsmI GAATG_Cn'
Cuts at: 3487 3513 4901 4994 3487
Size: 26 1388 93 5883
Fragments arranged by size:
5883 1388 93 26
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2049 2147 2269 2297 2467 2474
Size: 399 233 26 98 122 28 170 7
Cuts at: 2474 2490 2541 2766 2820 2841 2919 2946 3078
Size: 16 51 225 54 21 78 27 132
Cuts at: 3078 3147 3208 3477 3579 3678 3718 3755 3813
Size: 69 61 269 102 99 40 37 58
Cuts at: 3813 3979 4032 4100 4114 4154 4381 4593 4709
Size: 166 53 68 14 40 227 212 116
Cuts at: 4709 5451 5536 6607 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 269 233 227 225
212 170 166 132 122 116 102 99
98 85 78 69 68 61 58 54
53 51 40 40 37 28 27 26
21 16 14 7
BspEI T'CCGG_A
Cuts at: 7053 7053
Size: 7390
BspHI T'CATG_A
Cuts at: 5279 7009 5279
Size: 1730 5660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2514 2903 4745 844
Size: 1670 389 1842 3489
Fragments arranged by size:
3489 1842 1670 389
BsrBI CCG'CTC
Cuts at: 1394 1823 2081 4137 4453 5277 1394
Size: 429 258 2056 316 824 3507
Fragments arranged by size:
3507 2056 824 429 316 258
BsrDI GCAATG_nn'
Cuts at: 65 1248 3132 3329 5885 6059 65
Size: 1183 1884 197 2556 174 1396
Fragments arranged by size:
2556 1884 1396 1183 197 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2086 2988 4627 6034 6221 1700
Size: 110 276 902 1639 1407 187 2869
Fragments arranged by size:
2869 1639 1407 902 276 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7390
BssHII G'CGCG_C
Cuts at: 1925 2135 1925
Size: 210 7180
BssSI C'ACGA_G
Cuts at: 5450 6462 5450
Size: 1012 6378
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3343 3517 4020 4071 4158 4180 794
Size: 2549 174 503 51 87 22 4004
Fragments arranged by size:
4004 2549 503 174 87 51 22
BstBI TT'CG_AA
Cuts at: 6194 6194
Size: 7390
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7390
BstXI CCAn_nnnn'nTGG
Cuts at: 2596 2993 2596
Size: 397 6993
BstYI r'GATC_y
Cuts at: 1972 2173 2331 3961 4720 5087 5472 5489 6941
Size: 201 158 1630 759 367 385 17 1452
Cuts at: 6941 7027 7039 7385 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1630 1452 759 385 367 346 201
158 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4383 4383
Size: 7390
BtgI C'CryG_G
Cuts at: 513 1065 3259 3412 513
Size: 552 2194 153 4491
Fragments arranged by size:
4491 2194 552 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6651
Fragments arranged by size:
6651 683 56
BtsI GCAGTG_nn'
Cuts at: 2564 4060 4647 4906 5700 5720 2564
Size: 1496 587 259 794 20 4234
Fragments arranged by size:
4234 1496 794 587 259 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7390
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7355
DraI TTT'AAA
Cuts at: 4694 4694
Size: 7390
DraIII CAC_nnn'GTG
Cuts at: 4011 4180 4011
Size: 169 7221
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6397 809
Size: 507 5081 1802
Fragments arranged by size:
5081 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 2058 2362
Size: 54 1425 50 275 18 181 47 304
Cuts at: 2362 2586 2976 3246 3694 4555 4837 5726 7355
Size: 224 390 270 448 861 282 889 1629
Cuts at: 7355 8
Size: 43
Fragments arranged by size:
1629 1425 889 861 448 390 304 282
275 270 224 181 54 50 47 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3246 4837 1537
Size: 275 1434 1591 4090
Fragments arranged by size:
4090 1591 1434 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3045 5320 1353
Size: 1692 2275 3423
Fragments arranged by size:
3423 2275 1692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2073 5977 6351 6497 387
Size: 1064 138 484 3904 374 146 1280
Fragments arranged by size:
3904 1280 1064 484 374 146 138
EcoICRI GAG'CTC
Cuts at: 3145 3577 3753 3811 3145
Size: 432 176 58 6724
Fragments arranged by size:
6724 432 176 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2202 2309 2477 2555 3041 3368
Size: 393 498 72 107 168 78 486 327
Cuts at: 3368 3470 3542 3797 3893 3911 4259 4492 5453
Size: 102 72 255 96 18 348 233 961
Cuts at: 5453 6050 6837 7105 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 961 787 597 498 486 393 348
327 268 255 233 168 107 102 96
78 72 72 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3023 3191 3023
Size: 168 7222
EcoO109I rG'GnC_Cy
Cuts at: 2266 2390 2417 2593 2714 2817 2916 2943 4589
Size: 124 27 176 121 103 99 27 1646
Cuts at: 4589 2266
Size: 5067
Fragments arranged by size:
5067 1646 176 124 121 103 99 27
27
EcoRI G'AATT_C
Cuts at: 4699 5077 4699
Size: 378 7012
FspI TGC'GCA
Cuts at: 5896 5896
Size: 7390
HaeII r_GCGC'y
Cuts at: 1678 2034 2079 2214 3802 4225 6537 1678
Size: 356 45 135 1588 423 2312 2531
Fragments arranged by size:
2531 2312 1588 423 356 135 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 6031 6063 6105 6137 1373
Size: 32 135 32 4459 32 42 32 2626
Fragments arranged by size:
4459 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3010 3108 4734 4980 669
Size: 988 281 1072 98 1626 246 3079
Fragments arranged by size:
3079 1626 1072 988 281 246 98
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7390
HpaI GTT'AAC
Cuts at: 4980 4980
Size: 7390
KasI G'GCGC_C
Cuts at: 2030 2075 2030
Size: 45 7345
KpnI G_GTAC'C
Cuts at: 4715 4715
Size: 7390
MfeI C'AATT_G
Cuts at: 4989 4989
Size: 7390
MluI A'CGCG_T
Cuts at: 7266 7266
Size: 7390
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2167 2904 3780 4164 6504 6688 1305
Size: 862 737 876 384 2340 184 2007
Fragments arranged by size:
2340 2007 876 862 737 384 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2013 2364 2588 3696 10
Size: 54 1425 343 181 351 224 1108 3704
Fragments arranged by size:
3704 1425 1108 351 343 224 181 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3480 4013 5348 5707 5866 518
Size: 648 2314 533 1335 359 159 2042
Fragments arranged by size:
2314 2042 1335 648 533 359 159
MspA1I CmG'CkG
Cuts at: 1606 2398 2793 2853 4500 4806 5483 6631 6876
Size: 792 395 60 1647 306 677 1148 245
Cuts at: 6876 1606
Size: 2120
Fragments arranged by size:
2120 1647 1148 792 677 395 306 245
60
NaeI GCC'GGC
Cuts at: 2088 2088
Size: 7390
NarI GG'CG_CC
Cuts at: 2031 2076 2031
Size: 45 7345
NcoI C'CATG_G
Cuts at: 513 1065 3412 513
Size: 552 2347 4491
Fragments arranged by size:
4491 2347 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7390
NgoMIV G'CCGG_C
Cuts at: 2086 2086
Size: 7390
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7390
NotI GC'GGCC_GC
Cuts at: 4837 4837
Size: 7390
NsiI A_TGCA'T
Cuts at: 2010 3513 2010
Size: 1503 5887
NspI r_CATG'y
Cuts at: 1132 1229 3346 4018 4082 4472 4748 6293 1132
Size: 97 2117 672 64 390 276 1545 2229
Fragments arranged by size:
2229 2117 1545 672 390 276 97 64
PasI CC'CwG_GG
Cuts at: 1348 2604 2939 1348
Size: 1256 335 5799
Fragments arranged by size:
5799 1256 335
PciI A'CATG_T
Cuts at: 6289 6289
Size: 7390
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2699 3187 3346 1108
Size: 1591 488 159 5152
Fragments arranged by size:
5152 1591 488 159
PfoI T'CCnGG_A
Cuts at: 3186 3990 4236 3186
Size: 804 246 6340
Fragments arranged by size:
6340 804 246
PmeI GTTT'AAAC
Cuts at: 4694 4694
Size: 7390
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5459 5491 6972 7004 5459
Size: 32 1481 32 5845
Fragments arranged by size:
5845 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2390 2417 2714 2390
Size: 27 297 7066
Fragments arranged by size:
7066 297 27
PshAI GACnn'nnGTC
Cuts at: 1613 2415 3094 1613
Size: 802 679 5909
Fragments arranged by size:
5909 802 679
PsiI TTA'TAA
Cuts at: 4960 4960
Size: 7390
PspOMI G'GGCC_C
Cuts at: 2265 2470 2816 2915 2942 4110 4589 2265
Size: 205 346 99 27 1168 479 5066
Fragments arranged by size:
5066 1168 479 346 205 99 27
PspXI vC'TCGA_Gb
Cuts at: 3806 3806
Size: 7390
PstI C_TGCA'G
Cuts at: 830 2511 2632 4205 4264 4283 4742 830
Size: 1681 121 1573 59 19 459 3478
Fragments arranged by size:
3478 1681 1573 459 121 59 19
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7390
PvuII CAG'CTG
Cuts at: 4500 4500
Size: 7390
SacI G_AGCT'C
Cuts at: 3147 3579 3755 3813 3147
Size: 432 176 58 6724
Fragments arranged by size:
6724 432 176 58
SalI G'TCGA_C
Cuts at: 1936 4732 1936
Size: 2796 4594
SbfI CC_TGCA'GG
Cuts at: 4742 4742
Size: 7390
ScaI AGT'ACT
Cuts at: 5638 7350 5638
Size: 1712 5678
SfcI C'TryA_G
Cuts at: 826 1045 2507 2628 4201 4260 4279 4529 4738
Size: 219 1462 121 1573 59 19 250 209
Cuts at: 4738 5873 6554 6745 826
Size: 1135 681 191 1471
Fragments arranged by size:
1573 1471 1462 1135 681 250 219 209
191 121 59 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 4563 4563
Size: 7390
SfoI GGC'GCC
Cuts at: 2032 2077 2032
Size: 45 7345
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
SgrAI Cr'CCGG_yG
Cuts at: 1700 4627 1700
Size: 2927 4463
SmaI CCC'GGG
Cuts at: 1872 2025 2822 4587 4717 1872
Size: 153 797 1765 130 4545
Fragments arranged by size:
4545 1765 797 153 130
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3386 3599 3627 3806 5494 6395
Size: 197 926 1443 213 28 179 1688 901
Cuts at: 6395 6657 820
Size: 262 1553
Fragments arranged by size:
1688 1553 1443 926 901 262 213 197
179 28
SnaBI TAC'GTA
Cuts at: 493 5181 493
Size: 4688 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7390
SphI G_CATG'C
Cuts at: 1132 4018 4748 1132
Size: 2886 730 3774
Fragments arranged by size:
3774 2886 730
SspI AAT'ATT
Cuts at: 5 52 5314 5
Size: 47 5262 2081
Fragments arranged by size:
5262 2081 47
StuI AGG'CCT
Cuts at: 1517 2786 3164 4070 4250 1517
Size: 1269 378 906 180 4657
Fragments arranged by size:
4657 1269 906 378 180
StyI C'CwwG_G
Cuts at: 513 1065 2589 2973 3412 3691 4356 4829 513
Size: 552 1524 384 439 279 665 473 3074
Fragments arranged by size:
3074 1524 665 552 473 439 384 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3648 3958 4499 5417 5434 5587 5772
Size: 1255 2068 310 541 918 17 153 185
Cuts at: 5772 6217 325
Size: 445 1498
Fragments arranged by size:
2068 1498 1255 918 541 445 310 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5636 7348 96
Size: 274 80 33 51 1443 3659 1712 138
Fragments arranged by size:
3659 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7350 7382 7350
Size: 32 7358
Tth111I GACn'n_nGTC
Cuts at: 1316 3254 3557 3845 1316
Size: 1938 303 288 4861
Fragments arranged by size:
4861 1938 303 288
XbaI T'CTAG_A
Cuts at: 4726 4726
Size: 7390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3496 3496
Size: 7390
XhoI C'TCGA_G
Cuts at: 1943 3806 1943
Size: 1863 5527
XmaI C'CCGG_G
Cuts at: 1870 2023 2820 4585 4715 1870
Size: 153 797 1765 130 4545
Fragments arranged by size:
4545 1765 797 153 130
XmnI GAAnn'nnTTC
Cuts at: 5519 5519
Size: 7390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5198 276
Size: 53 83 186 936 3664 2468
Fragments arranged by size:
3664 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I
EcoRV FalI FseI FspAI NruI PacI PmlI PsrI
RsrII SacII SanDI SapI SexAI SrfI SwaI