Restriction map for
pFN21AB3674
(Circular) (Six-base) MAPSORT of: pFN21AB3674.seq Check: 2904 from: 1 to: 6250
pFN21AB3674 6250 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2103 2103
Size: 6250
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4060 278
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3593 1937
Size: 1656 4594
Acc65I G'GTAC_C
Cuts at: 3571 3571
Size: 6250
AclI AA'CG_TT
Cuts at: 1563 4377 4750 1563
Size: 2814 373 3063
Fragments arranged by size:
3063 2814 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4313 5697 5965 1632
Size: 2681 1384 268 1917
Fragments arranged by size:
2681 1917 1384 268
AfeI AGC'GCT
Cuts at: 2463 2463
Size: 6250
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6053
AflIII A'CryG_T
Cuts at: 1203 3275 5149 6126 1203
Size: 2072 1874 977 1327
Fragments arranged by size:
2072 1874 1327 977
AgeI A'CCGG_T
Cuts at: 5081 5081
Size: 6250
AhdI GACnn_n'nnGTC
Cuts at: 2941 4979 2941
Size: 2038 4212
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6250
AlwNI CAG_nnn'CTG
Cuts at: 2204 2374 2494 2793 3993 5565 2204
Size: 170 120 299 1200 1572 2889
Fragments arranged by size:
2889 1572 1200 299 170 120
ApaLI G'TGCA_C
Cuts at: 4307 5463 4307
Size: 1156 5094
ApoI r'AATT_y
Cuts at: 1493 3353 3559 3785 3937 6046 1493
Size: 1860 206 226 152 2109 1697
Fragments arranged by size:
2109 1860 1697 226 206 152
AseI AT'TA_AT
Cuts at: 160 1032 4804 160
Size: 872 3772 1606
Fragments arranged by size:
3772 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
AvaI C'yCGr_G
Cuts at: 1870 1943 3575 1870
Size: 73 1632 4545
Fragments arranged by size:
4545 1632 73
BamHI G'GATC_C
Cuts at: 3580 3947 3580
Size: 367 5883
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2260 3571 5026 618
Size: 325 217 627 473 1311 1455 1842
Fragments arranged by size:
1842 1455 1311 627 473 325 217
BanII G_rGCy'C
Cuts at: 1391 2893 3569 1391
Size: 1502 676 4072
Fragments arranged by size:
4072 1502 676
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2476 2506 928
Size: 971 577 30 4672
Fragments arranged by size:
4672 971 577 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4441 4475 1903
Size: 34 2504 34 3678
Fragments arranged by size:
3678 2504 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4142 5352 4142
Size: 1210 5040
BclI T'GATC_A
Cuts at: 1548 3024 1548
Size: 1476 4774
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4861 136
Size: 107 122 71 631 605 337 2852 1525
Fragments arranged by size:
2852 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6245 6245
Size: 6250
BlpI GC'TnA_GC
Cuts at: 3510 3667 3510
Size: 157 6093
Bme1580I G_kGCm'C
Cuts at: 1790 3044 4311 5467 1790
Size: 1254 1267 1156 2573
Fragments arranged by size:
2573 1267 1254 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6250
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3981 4939 442
Size: 416 53 83 399 24 2564 958 1753
Fragments arranged by size:
2564 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6250
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2480 3470 2480
Size: 990 5260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2877 4910 1239
Size: 1638 2033 2579
Fragments arranged by size:
2579 2033 1638
Bpu10I CC'TnA_GC
Cuts at: 2301 3084 3222 3232 2301
Size: 783 138 10 5319
Fragments arranged by size:
5319 783 138 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4375 5240 5538 4375
Size: 865 298 5087
Fragments arranged by size:
5087 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2503 4913 882
Size: 617 1004 2410 2219
Fragments arranged by size:
2410 2219 1004 617
BsaAI yAC'GTr
Cuts at: 493 1204 4041 493
Size: 711 2837 2702
Fragments arranged by size:
2837 2702 711
BsaBI GATnn'nnATC
Cuts at: 6244 6244
Size: 6250
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4057 4439 5074 275
Size: 53 83 186 936 2524 382 635 1451
Fragments arranged by size:
2524 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4683 5081 5355 5502 5913 4683
Size: 398 274 147 411 5020
Fragments arranged by size:
5020 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6025
Fragments arranged by size:
6025 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2076 2079 2655 3525 1186
Size: 890 3 576 870 3911
Fragments arranged by size:
3911 890 870 576 3
BseYI C'CCAG_C
Cuts at: 2296 5453 5949 2296
Size: 3157 496 2597
Fragments arranged by size:
3157 2597 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 1163
Size: 573 345 5332
Fragments arranged by size:
5332 573 345
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3700 4461 5489 1540
Size: 275 186 1699 761 1028 2301
Fragments arranged by size:
2301 1699 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4311 4396 5467 4311
Size: 85 1071 5094
Fragments arranged by size:
5094 1071 85
BsmI GAATG_Cn'
Cuts at: 3761 3854 3761
Size: 93 6157
BsmBI CGTCTCn'nnnn_
Cuts at: 2665 2665
Size: 6250
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2893 3044 3569 4311 4396 5467 1391
Size: 399 1103 151 525 742 85 1071 2174
Fragments arranged by size:
2174 1103 1071 742 525 399 151 85
BspEI T'CCGG_A
Cuts at: 5913 5913
Size: 6250
BspHI T'CATG_A
Cuts at: 4139 5869 4139
Size: 1730 4520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2033 2103 3605 844
Size: 1189 70 1502 3489
Fragments arranged by size:
3489 1502 1189 70
BsrBI CCG'CTC
Cuts at: 1394 1823 4137 1394
Size: 429 2314 3507
Fragments arranged by size:
3507 2314 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2932 3066 4745 4919 65
Size: 1183 1684 134 1679 174 1396
Fragments arranged by size:
1684 1679 1396 1183 174 134
BsrFI r'CCGG_y
Cuts at: 1700 1810 2257 4894 5081 1700
Size: 110 447 2637 187 2869
Fragments arranged by size:
2869 2637 447 187 110
BsrGI T'GTAC_A
Cuts at: 96 2149 2158 96
Size: 2053 9 4188
Fragments arranged by size:
4188 2053 9
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6250
BssSI C'ACGA_G
Cuts at: 4310 5322 4310
Size: 1012 5238
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2204 794
Size: 1410 4840
BstBI TT'CG_AA
Cuts at: 5054 5054
Size: 6250
BstEII G'GTnAC_C
Cuts at: 1184 2045 1184
Size: 861 5389
BstXI CCAn_nnnn'nTGG
Cuts at: 2741 2741
Size: 6250
BstYI r'GATC_y
Cuts at: 1972 2437 3031 3580 3947 4332 4349 5801 5887
Size: 465 594 549 367 385 17 1452 86
Cuts at: 5887 5899 6245 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 594 549 465 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4759
Fragments arranged by size:
4759 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5511
Fragments arranged by size:
5511 683 56
BtsI GCAGTG_nn'
Cuts at: 3766 4560 4580 3766
Size: 794 20 5436
Fragments arranged by size:
5436 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6215
DraI TTT'AAA
Cuts at: 3554 3554
Size: 6250
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5257 809
Size: 507 3941 1802
Fragments arranged by size:
3941 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2823 3697 4586
Size: 54 1425 50 275 18 993 874 889
Cuts at: 4586 6215 8
Size: 1629 43
Fragments arranged by size:
1629 1425 993 889 874 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3697 1537
Size: 275 1885 4090
Fragments arranged by size:
4090 1885 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2179 3129 3307 4180 1353
Size: 826 950 178 873 3423
Fragments arranged by size:
3423 950 873 826 178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4837 5211 5357 387
Size: 1064 138 3248 374 146 1280
Fragments arranged by size:
3248 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2877 4313 4910 5697 5965 1239
Size: 393 1245 1436 597 787 268 1524
Fragments arranged by size:
1524 1436 1245 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3104 3104
Size: 6250
EcoRI G'AATT_C
Cuts at: 3559 3937 3559
Size: 378 5872
EcoRV GAT'ATC
Cuts at: 3269 3269
Size: 6250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2717 3247 2717
Size: 530 5720
FspI TGC'GCA
Cuts at: 4756 4756
Size: 6250
HaeII r_GCGC'y
Cuts at: 1678 2465 3084 5397 1678
Size: 787 619 2313 2531
Fragments arranged by size:
2531 2313 787 619
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2927 2959 3016 3048 4891
Size: 32 135 32 1355 32 57 32 1843
Cuts at: 4891 4923 4965 4997 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1843 1355 135 57 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3594 3840 669
Size: 988 281 1656 246 3079
Fragments arranged by size:
3079 1656 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6250
HpaI GTT'AAC
Cuts at: 3840 3840
Size: 6250
KpnI G_GTAC'C
Cuts at: 3575 3575
Size: 6250
MfeI C'AATT_G
Cuts at: 3849 3849
Size: 6250
MluI A'CGCG_T
Cuts at: 6126 6126
Size: 6250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2605 2997 3415 5364 5548 1305
Size: 1300 392 418 1949 184 2007
Fragments arranged by size:
2007 1949 1300 418 392 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2825 10
Size: 54 1425 343 993 3435
Fragments arranged by size:
3435 1425 993 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2553 3278 3280 4208 4567 4726 518
Size: 648 1387 725 2 928 359 159 2042
Fragments arranged by size:
2042 1387 928 725 648 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2147 2183 2201 2210 2285 2321 2374 2741
Size: 541 36 18 9 75 36 53 367
Cuts at: 2741 3666 4343 5491 5736 1606
Size: 925 677 1148 245 2120
Fragments arranged by size:
2120 1148 925 677 541 367 245 75
53 36 36 18 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4759
Fragments arranged by size:
4759 939 552
NdeI CA'TA_TG
Cuts at: 387 2856 387
Size: 2469 3781
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6250
NotI GC'GGCC_GC
Cuts at: 3697 3697
Size: 6250
NspI r_CATG'y
Cuts at: 1132 1229 3279 3608 5153 1132
Size: 97 2050 329 1545 2229
Fragments arranged by size:
2229 2050 1545 329 97
PasI CC'CwG_GG
Cuts at: 1348 2228 1348
Size: 880 5370
PciI A'CATG_T
Cuts at: 3275 5149 3275
Size: 1874 4376
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2447 2494 1108
Size: 1339 47 4864
Fragments arranged by size:
4864 1339 47
PfoI T'CCnGG_A
Cuts at: 2275 2517 2275
Size: 242 6008
PmeI GTTT'AAAC
Cuts at: 3554 3554
Size: 6250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4319 4351 5832 5864 4319
Size: 32 1481 32 4705
Fragments arranged by size:
4705 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3104 3104
Size: 6250
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6250
PsiI TTA'TAA
Cuts at: 3820 3820
Size: 6250
PstI C_TGCA'G
Cuts at: 830 2035 2209 2215 2290 3602 830
Size: 1205 174 6 75 1312 3478
Fragments arranged by size:
3478 1312 1205 174 75 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6250
PvuII CAG'CTG
Cuts at: 2183 2210 2285 2321 2374 2741 2183
Size: 27 75 36 53 367 5692
Fragments arranged by size:
5692 367 75 53 36 27
SalI G'TCGA_C
Cuts at: 1936 3592 1936
Size: 1656 4594
SbfI CC_TGCA'GG
Cuts at: 3602 3602
Size: 6250
ScaI AGT'ACT
Cuts at: 4498 6210 4498
Size: 1712 4538
SfcI C'TryA_G
Cuts at: 826 1045 2031 2205 2211 2286 2325 3598 4733
Size: 219 986 174 6 75 39 1273 1135
Cuts at: 4733 5414 5605 826
Size: 681 191 1471
Fragments arranged by size:
1471 1273 1135 986 681 219 191 174
75 39 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6250
SmaI CCC'GGG
Cuts at: 1872 3577 1872
Size: 1705 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4354 5255 5517 820
Size: 197 926 2411 901 262 1553
Fragments arranged by size:
2411 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4041 493
Size: 3548 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6250
SphI G_CATG'C
Cuts at: 1132 3608 1132
Size: 2476 3774
SspI AAT'ATT
Cuts at: 5 52 2985 4174 5
Size: 47 2933 1189 2081
Fragments arranged by size:
2933 2081 1189 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6250
StyI C'CwwG_G
Cuts at: 513 1065 2004 3689 513
Size: 552 939 1685 3074
Fragments arranged by size:
3074 1685 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4277 4294 4447 4632 5077 325
Size: 1255 2697 17 153 185 445 1498
Fragments arranged by size:
2697 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2149 2158 2557
Size: 274 80 33 51 1443 172 9 399
Cuts at: 2557 3130 4496 6208 96
Size: 573 1366 1712 138
Fragments arranged by size:
1712 1443 1366 573 399 274 172 138
80 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3058 3090 3432 3464 6210 6242 3058
Size: 32 342 32 2746 32 3066
Fragments arranged by size:
3066 2746 342 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6250
XbaI T'CTAG_A
Cuts at: 3586 3586
Size: 6250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3013 3013
Size: 6250
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6250
XmaI C'CCGG_G
Cuts at: 1870 3575 1870
Size: 1705 4545
XmnI GAAnn'nnTTC
Cuts at: 3168 3408 4379 3168
Size: 240 971 5039
Fragments arranged by size:
5039 971 240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4058 276
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI