Restriction map for
pFN21AB3666
(Circular) (Six-base) MAPSORT of: pFN21AB3666.seq Check: 7060 from: 1 to: 6919
pFN21AB3666 6919 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3676 3676
Size: 6919
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2046 4729 278
Size: 53 83 186 936 510 2683 2468
Fragments arranged by size:
2683 2468 936 510 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4262 1937
Size: 2325 4594
Acc65I G'GTAC_C
Cuts at: 4240 4240
Size: 6919
AclI AA'CG_TT
Cuts at: 1563 2457 5046 5419 1563
Size: 894 2589 373 3063
Fragments arranged by size:
3063 2589 894 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3603 3825 4040 4982 6366 6634 1632
Size: 1971 222 215 942 1384 268 1917
Fragments arranged by size:
1971 1917 1384 942 268 222 215
AflII C'TTAA_G
Cuts at: 820 1017 2438 820
Size: 197 1421 5301
Fragments arranged by size:
5301 1421 197
AflIII A'CryG_T
Cuts at: 1203 5818 6795 1203
Size: 4615 977 1327
Fragments arranged by size:
4615 1327 977
AgeI A'CCGG_T
Cuts at: 5750 5750
Size: 6919
AhdI GACnn_n'nnGTC
Cuts at: 5648 5648
Size: 6919
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6919
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3735 3767 3735
Size: 32 6887
AlwNI CAG_nnn'CTG
Cuts at: 3902 4118 4662 6234 3902
Size: 216 544 1572 4587
Fragments arranged by size:
4587 1572 544 216
ApaLI G'TGCA_C
Cuts at: 4976 6132 4976
Size: 1156 5763
ApoI r'AATT_y
Cuts at: 1493 2318 2361 2394 2637 3059 3138 4228 4454
Size: 825 43 33 243 422 79 1090 226
Cuts at: 4454 4606 6715 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1090 825 422 243 226 152
79 43 33
AseI AT'TA_AT
Cuts at: 160 1032 5473 160
Size: 872 4441 1606
Fragments arranged by size:
4441 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6919
AvaI C'yCGr_G
Cuts at: 1870 1943 3924 4244 1870
Size: 73 1981 320 4545
Fragments arranged by size:
4545 1981 320 73
BamHI G'GATC_C
Cuts at: 4249 4616 4249
Size: 367 6552
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2500 3664 4240 5695
Size: 325 217 627 273 440 1164 576 1455
Cuts at: 5695 618
Size: 1842
Fragments arranged by size:
1842 1455 1164 627 576 440 325 273
217
BanII G_rGCy'C
Cuts at: 1391 2123 2550 3324 3684 4238 1391
Size: 732 427 774 360 554 4072
Fragments arranged by size:
4072 774 732 554 427 360
BbeI G_GCGC'C
Cuts at: 2064 2064
Size: 6919
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2038 3446 3633 928
Size: 971 139 1408 187 4214
Fragments arranged by size:
4214 1408 971 187 139
BbvCI CC'TCA_GC
Cuts at: 3702 3702
Size: 6919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5110 5144 1903
Size: 34 3173 34 3678
Fragments arranged by size:
3678 3173 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4811 6021 4811
Size: 1210 5709
BclI T'GATC_A
Cuts at: 1548 2512 1548
Size: 964 5955
BfrBI ATG'CAT
Cuts at: 2926 4024 2926
Size: 1098 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5530 136
Size: 107 122 71 631 605 337 3521 1525
Fragments arranged by size:
3521 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6914 6914
Size: 6919
BlpI GC'TnA_GC
Cuts at: 4336 4336
Size: 6919
Bme1580I G_kGCm'C
Cuts at: 1790 4980 6136 1790
Size: 3190 1156 2573
Fragments arranged by size:
3190 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6919
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4650 5608 442
Size: 416 53 83 399 24 3233 958 1753
Fragments arranged by size:
3233 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3605 1056
Size: 2549 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2021 3747 3796 5579 1239
Size: 782 1726 49 1783 2579
Fragments arranged by size:
2579 1783 1726 782 49
Bpu10I CC'TnA_GC
Cuts at: 3702 3702
Size: 6919
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2260 5044 5909 6207 2260
Size: 2784 865 298 2972
Fragments arranged by size:
2972 2784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2179 5582 882
Size: 617 680 3403 2219
Fragments arranged by size:
3403 2219 680 617
BsaAI yAC'GTr
Cuts at: 493 1204 4710 493
Size: 711 3506 2702
Fragments arranged by size:
3506 2702 711
BsaBI GATnn'nnATC
Cuts at: 6913 6913
Size: 6919
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2061 4726 5108
Size: 53 83 186 936 510 18 2665 382
Cuts at: 5108 5743 275
Size: 635 1451
Fragments arranged by size:
2665 1451 936 635 510 382 186 83
53 18
BsaWI w'CCGG_w
Cuts at: 5352 5750 6024 6171 6582 5352
Size: 398 274 147 411 5689
Fragments arranged by size:
5689 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3735 3765 1178
Size: 30 165 30 2332 30 4332
Fragments arranged by size:
4332 2332 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6919
BseYI C'CCAG_C
Cuts at: 3323 6122 6618 3323
Size: 2799 496 3624
Fragments arranged by size:
3624 2799 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4369 5130 6158 1540
Size: 275 186 2368 761 1028 2301
Fragments arranged by size:
2368 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2123 2550 4980 5065 6136 2123
Size: 427 2430 85 1071 2906
Fragments arranged by size:
2906 2430 1071 427 85
BsmI GAATG_Cn'
Cuts at: 3789 4430 4523 3789
Size: 641 93 6185
Fragments arranged by size:
6185 641 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2550 3324 3684 4238 4980 5065
Size: 399 333 427 774 360 554 742 85
Cuts at: 5065 6136 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 774 742 554 427 399 360
333 85
BspEI T'CCGG_A
Cuts at: 6582 6582
Size: 6919
BspHI T'CATG_A
Cuts at: 3349 3424 4808 6538 3349
Size: 75 1384 1730 3730
Fragments arranged by size:
3730 1730 1384 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 3676 3891 4274 844
Size: 1316 1516 215 383 3489
Fragments arranged by size:
3489 1516 1316 383 215
BsrBI CCG'CTC
Cuts at: 1394 1823 4806 1394
Size: 429 2983 3507
Fragments arranged by size:
3507 2983 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5414 5588 65
Size: 1183 4166 174 1396
Fragments arranged by size:
4166 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5563 5750 1700
Size: 110 3753 187 2869
Fragments arranged by size:
3753 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6919
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6919
BssSI C'ACGA_G
Cuts at: 3630 4979 5991 3630
Size: 1349 1012 4558
Fragments arranged by size:
4558 1349 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6919
BstBI TT'CG_AA
Cuts at: 5723 5723
Size: 6919
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6919
BstYI r'GATC_y
Cuts at: 1972 2653 4249 4616 5001 5018 6470 6556 6568
Size: 681 1596 367 385 17 1452 86 12
Cuts at: 6568 6914 1972
Size: 346 1977
Fragments arranged by size:
1977 1596 1452 681 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2520 3270 2520
Size: 750 6169
BtgI C'CryG_G
Cuts at: 513 1065 2004 2721 3018 513
Size: 552 939 717 297 4414
Fragments arranged by size:
4414 939 717 552 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6180
Fragments arranged by size:
6180 683 56
BtsI GCAGTG_nn'
Cuts at: 2595 4435 5229 5249 2595
Size: 1840 794 20 4265
Fragments arranged by size:
4265 1840 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6919
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6884
DraI TTT'AAA
Cuts at: 4223 4223
Size: 6919
DraIII CAC_nnn'GTG
Cuts at: 3197 3197
Size: 6919
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5926 809
Size: 507 4610 1802
Fragments arranged by size:
4610 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2871 4366 5255
Size: 54 1425 50 275 18 1041 1495 889
Cuts at: 5255 6884 8
Size: 1629 43
Fragments arranged by size:
1629 1495 1425 1041 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4366 1537
Size: 275 2554 4090
Fragments arranged by size:
4090 2554 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2804 3000 3312 3801 4059 4849 1353
Size: 1451 196 312 489 258 790 3423
Fragments arranged by size:
3423 1451 790 489 312 258 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5506 5880 6026 387
Size: 1064 138 3917 374 146 1280
Fragments arranged by size:
3917 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2121 2548 2121
Size: 427 6492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2021 3603 3747 3796 3825 4040 4982
Size: 393 389 1582 144 49 29 215 942
Cuts at: 4982 5579 6366 6634 1239
Size: 597 787 268 1524
Fragments arranged by size:
1582 1524 942 787 597 393 389 268
215 144 49 29
EcoO109I rG'GnC_Cy
Cuts at: 2149 2149
Size: 6919
EcoRI G'AATT_C
Cuts at: 4228 4606 4228
Size: 378 6541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3054 4070 3054
Size: 1016 5903
FspI TGC'GCA
Cuts at: 5425 5425
Size: 6919
HaeII r_GCGC'y
Cuts at: 1678 2015 2064 6066 1678
Size: 337 49 4002 2531
Fragments arranged by size:
4002 2531 337 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5560 5592 5634 5666 1373
Size: 32 135 32 3988 32 42 32 2626
Fragments arranged by size:
3988 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2779 4263 4509 669
Size: 988 281 841 1484 246 3079
Fragments arranged by size:
3079 1484 988 841 281 246
HindIII A'AGCT_T
Cuts at: 748 2339 748
Size: 1591 5328
HpaI GTT'AAC
Cuts at: 2779 4509 2779
Size: 1730 5189
KasI G'GCGC_C
Cuts at: 2060 2060
Size: 6919
KpnI G_GTAC'C
Cuts at: 4244 4244
Size: 6919
MfeI C'AATT_G
Cuts at: 3507 4518 3507
Size: 1011 5908
MluI A'CGCG_T
Cuts at: 6795 6795
Size: 6919
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3070 3721 6033 6217 1305
Size: 1765 651 2312 184 2007
Fragments arranged by size:
2312 2007 1765 651 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2873 10
Size: 54 1425 343 1041 4056
Fragments arranged by size:
4056 1425 1041 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4877 5236 5395 518
Size: 648 3711 359 159 2042
Fragments arranged by size:
3711 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2112 3333 3623 3692 4078 4151 4335
Size: 471 35 1221 290 69 386 73 184
Cuts at: 4335 5012 6160 6405 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1221 1148 677 471 386 290 245
184 73 69 35
NarI GG'CG_CC
Cuts at: 2061 2061
Size: 6919
NcoI C'CATG_G
Cuts at: 513 1065 2004 2721 513
Size: 552 939 717 4711
Fragments arranged by size:
4711 939 717 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6919
NheI G'CTAG_C
Cuts at: 1052 3601 1052
Size: 2549 4370
NotI GC'GGCC_GC
Cuts at: 4366 4366
Size: 6919
NsiI A_TGCA'T
Cuts at: 2928 4026 2928
Size: 1098 5821
NspI r_CATG'y
Cuts at: 1132 1229 4277 5822 1132
Size: 97 3048 1545 2229
Fragments arranged by size:
3048 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6919
PciI A'CATG_T
Cuts at: 5818 5818
Size: 6919
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3197 1108
Size: 2089 4830
PfoI T'CCnGG_A
Cuts at: 2037 4200 2037
Size: 2163 4756
PmeI GTTT'AAAC
Cuts at: 4223 4223
Size: 6919
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3735 3767 4988 5020 6501 6533 3735
Size: 32 1221 32 1481 32 4121
Fragments arranged by size:
4121 1481 1221 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2149 2149
Size: 6919
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6919
PsiI TTA'TAA
Cuts at: 4489 4489
Size: 6919
PstI C_TGCA'G
Cuts at: 830 4271 830
Size: 3441 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6919
PvuII CAG'CTG
Cuts at: 2077 3623 4078 4151 2077
Size: 1546 455 73 4845
Fragments arranged by size:
4845 1546 455 73
SacI G_AGCT'C
Cuts at: 2123 2550 2123
Size: 427 6492
SalI G'TCGA_C
Cuts at: 1936 4261 1936
Size: 2325 4594
SapI GCTCTTCn'nnn_
Cuts at: 3312 3312
Size: 6919
SbfI CC_TGCA'GG
Cuts at: 4271 4271
Size: 6919
ScaI AGT'ACT
Cuts at: 5167 6879 5167
Size: 1712 5207
SfcI C'TryA_G
Cuts at: 826 1045 2505 3379 4267 5402 6083 6274 826
Size: 219 1460 874 888 1135 681 191 1471
Fragments arranged by size:
1471 1460 1135 888 874 681 219 191
SfoI GGC'GCC
Cuts at: 2062 2062
Size: 6919
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6919
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6919
SmaI CCC'GGG
Cuts at: 1872 4246 1872
Size: 2374 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2275 2438 5023 5924 6186 820
Size: 197 926 332 163 2585 901 262 1553
Fragments arranged by size:
2585 1553 926 901 332 262 197 163
SnaBI TAC'GTA
Cuts at: 493 4710 493
Size: 4217 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6919
SphI G_CATG'C
Cuts at: 1132 4277 1132
Size: 3145 3774
SspI AAT'ATT
Cuts at: 5 52 3074 3994 4843 5
Size: 47 3022 920 849 2081
Fragments arranged by size:
3022 2081 920 849 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6919
StyI C'CwwG_G
Cuts at: 513 1065 2004 2496 2721 4358 513
Size: 552 939 492 225 1637 3074
Fragments arranged by size:
3074 1637 939 552 492 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2467 4946 4963 5116 5301 5746 325
Size: 1255 887 2479 17 153 185 445 1498
Fragments arranged by size:
2479 1498 1255 887 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3392 5165 6877
Size: 274 80 33 51 1443 1415 1773 1712
Cuts at: 6877 96
Size: 138
Fragments arranged by size:
1773 1712 1443 1415 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3520 3552 6879 6911 3520
Size: 32 3327 32 3528
Fragments arranged by size:
3528 3327 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6919
XbaI T'CTAG_A
Cuts at: 3313 4255 3313
Size: 942 5977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3161 3439 3161
Size: 278 6641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6919
XmaI C'CCGG_G
Cuts at: 1870 4244 1870
Size: 2374 4545
XmnI GAAnn'nnTTC
Cuts at: 2430 3345 5048 2430
Size: 915 1703 4301
Fragments arranged by size:
4301 1703 915
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2044 4727 276
Size: 53 83 186 936 510 2683 2468
Fragments arranged by size:
2683 2468 936 510 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BplI BsiWI BsmBI
BstXI BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI