Restriction map for
pFN21AB3657
(Circular) (Six-base) MAPSORT of: pFN21AB3657.seq Check: 8839 from: 1 to: 5785
pFN21AB3657 5785 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2287 2287
Size: 5785
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2850 3128 1937
Size: 913 278 4594
Fragments arranged by size:
4594 913 278
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2516 2684 3848 5232 5500 1632
Size: 884 168 1164 1384 268 1917
Fragments arranged by size:
1917 1384 1164 884 268 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 2149 4684 5661 1203
Size: 946 2535 977 1327
Fragments arranged by size:
2535 1327 977 946
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2656 2688 2656
Size: 32 5753
AlwNI CAG_nnn'CTG
Cuts at: 2041 2083 3528 5100 2041
Size: 42 1445 1572 2726
Fragments arranged by size:
2726 1572 1445 42
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 2297 2403 3094 3320 3472 5581 1493
Size: 804 106 691 226 152 2109 1697
Fragments arranged by size:
2109 1697 804 691 226 152 106
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 3110 1870
Size: 73 123 1044 4545
Fragments arranged by size:
4545 1044 123 73
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2062 2102 2831 3106 4561
Size: 325 217 627 275 40 729 275 1455
Cuts at: 4561 618
Size: 1842
Fragments arranged by size:
1842 1455 729 627 325 275 275 217
40
BanII G_rGCy'C
Cuts at: 1391 2099 2575 3104 1391
Size: 708 476 529 4072
Fragments arranged by size:
4072 708 529 476
BbeI G_GCGC'C
Cuts at: 2106 2106
Size: 5785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2490 928
Size: 971 591 4223
Fragments arranged by size:
4223 971 591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 2974 1548
Size: 1426 4359
BfrBI ATG'CAT
Cuts at: 2599 2637 2599
Size: 38 5747
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4396 136
Size: 107 122 71 631 605 2724 1525
Fragments arranged by size:
2724 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2338 5780 2338
Size: 3442 2343
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 2067 3846 5002 1790
Size: 277 1779 1156 2573
Fragments arranged by size:
2573 1779 1156 277
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3516 4474 442
Size: 416 53 83 399 24 2099 958 1753
Fragments arranged by size:
2099 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2073 2155 2312 2879 4445 1239
Size: 834 82 157 567 1566 2579
Fragments arranged by size:
2579 1566 834 567 157 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 4775 5073 3910
Size: 865 298 4622
Fragments arranged by size:
4622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2292 4448 882
Size: 617 793 2156 2219
Fragments arranged by size:
2219 2156 793 617
BsaAI yAC'GTr
Cuts at: 493 1204 2150 2551 3576 493
Size: 711 946 401 1025 2702
Fragments arranged by size:
2702 1025 946 711 401
BsaBI GATnn'nnATC
Cuts at: 2310 5779 2310
Size: 3469 2316
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 3592 3974 4609
Size: 53 83 186 936 570 1489 382 635
Cuts at: 4609 275
Size: 1451
Fragments arranged by size:
1489 1451 936 635 570 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5560
Fragments arranged by size:
5560 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5785
BseYI C'CCAG_C
Cuts at: 4988 5484 4988
Size: 496 5289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3846 3931 5002 3846
Size: 85 1071 4629
Fragments arranged by size:
4629 1071 85
BsmI GAATG_Cn'
Cuts at: 3296 3389 3296
Size: 93 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2099 2575 3104 3846 3931 5002
Size: 399 277 32 476 529 742 85 1071
Cuts at: 5002 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 529 476 399 277 85
32
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2287 3140 844
Size: 1443 853 3489
Fragments arranged by size:
3489 1443 853
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4280 4454 65
Size: 1183 3032 174 1396
Fragments arranged by size:
3032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 4429 4616 1700
Size: 110 221 2398 187 2869
Fragments arranged by size:
2869 2398 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2710 794
Size: 1916 3869
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 2317 1184
Size: 1133 4652
BstXI CCAn_nnnn'nTGG
Cuts at: 2842 2842
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 2338 3115 3482 3867 3884 5336 5422 5434
Size: 366 777 367 385 17 1452 86 12
Cuts at: 5434 5780 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 777 385 367 366 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 2163 3301 4095 4115 2163
Size: 1138 794 20 3833
Fragments arranged by size:
3833 1138 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DraIII CAC_nnn'GTG
Cuts at: 2562 2562
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2553 2643 2961
Size: 54 1425 50 275 18 723 90 318
Cuts at: 2961 3232 4121 5750 8
Size: 271 889 1629 43
Fragments arranged by size:
1629 1425 889 723 318 275 271 90
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3066 3715 1353
Size: 1713 649 3423
Fragments arranged by size:
3423 1713 649
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2155 2312 2516 2684 2879 3848
Size: 393 441 82 157 204 168 195 969
Cuts at: 3848 4445 5232 5500 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 969 787 597 441 393 268 204
195 168 157 82
EcoO109I rG'GnC_Cy
Cuts at: 2452 2995 2452
Size: 543 5242
EcoRI G'AATT_C
Cuts at: 2297 3094 3472 2297
Size: 797 378 4610
Fragments arranged by size:
4610 797 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 5785
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2106 4932 1678
Size: 428 2826 2531
Fragments arranged by size:
2826 2531 428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 2698 2730 3065
Size: 32 135 32 1037 32 57 32 335
Cuts at: 3065 3097 4426 4458 4500 4532 1373
Size: 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1329 1037 335 135 57 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2655 2851 3129 3375 669
Size: 988 281 717 196 278 246 3079
Fragments arranged by size:
3079 988 717 281 278 246 196
HindIII A'AGCT_T
Cuts at: 748 2366 748
Size: 1618 4167
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KasI G'GCGC_C
Cuts at: 2102 2102
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 4899 5083 1305
Size: 939 2655 184 2007
Fragments arranged by size:
2655 2007 939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2555 10
Size: 54 1425 343 723 3240
Fragments arranged by size:
3240 1425 723 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 2529 3743 4102 4261 518
Size: 648 1081 282 1214 359 159 2042
Fragments arranged by size:
2042 1214 1081 648 359 282 159
MspA1I CmG'CkG
Cuts at: 1606 2435 2900 3201 3878 5026 5271 1606
Size: 829 465 301 677 1148 245 2120
Fragments arranged by size:
2120 1148 829 677 465 301 245
NarI GG'CG_CC
Cuts at: 2103 2103
Size: 5785
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5233
NdeI CA'TA_TG
Cuts at: 387 2235 387
Size: 1848 3937
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NsiI A_TGCA'T
Cuts at: 2601 2639 2601
Size: 38 5747
NspI r_CATG'y
Cuts at: 1132 1229 2921 3143 4688 1132
Size: 97 1692 222 1545 2229
Fragments arranged by size:
2229 1692 1545 222 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2329 2562 1108
Size: 1221 233 4331
Fragments arranged by size:
4331 1221 233
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2995 2452
Size: 543 5242
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2208 2240 2208
Size: 32 5753
PstI C_TGCA'G
Cuts at: 830 3137 830
Size: 2307 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2435 2435
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 2849 3127 1936
Size: 913 278 4594
Fragments arranged by size:
4594 913 278
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 2209 3133 4268 4949 5140 826
Size: 219 1164 924 1135 681 191 1471
Fragments arranged by size:
1471 1164 1135 924 681 219 191
SfoI GGC'GCC
Cuts at: 2104 2104
Size: 5785
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3889 4790 5052 820
Size: 197 926 1946 901 262 1553
Fragments arranged by size:
1946 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 3224 513
Size: 552 2159 3074
Fragments arranged by size:
3074 2159 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3829 3982 4167 4612 325
Size: 1255 2232 17 153 185 445 1498
Fragments arranged by size:
2232 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4031 5743 96
Size: 274 80 33 51 1443 2054 1712 138
Fragments arranged by size:
2054 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI