Restriction map for
pFN21AB3655
(Circular) (Six-base) MAPSORT of: pFN21AB3655.seq Check: 6008 from: 1 to: 6388
pFN21AB3655 6388 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2980 2980
Size: 6388
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3731 1937
Size: 1794 4594
Acc65I G'GTAC_C
Cuts at: 3709 3709
Size: 6388
AclI AA'CG_TT
Cuts at: 1563 4515 4888 1563
Size: 2952 373 3063
Fragments arranged by size:
3063 2952 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2427 2982 4451 5835 6103 1632
Size: 795 555 1469 1384 268 1917
Fragments arranged by size:
1917 1469 1384 795 555 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6191
AflIII A'CryG_T
Cuts at: 1203 2808 5287 6264 1203
Size: 1605 2479 977 1327
Fragments arranged by size:
2479 1605 1327 977
AgeI A'CCGG_T
Cuts at: 5219 5219
Size: 6388
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 2342 1166
Size: 1176 5212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6388
AlwNI CAG_nnn'CTG
Cuts at: 2303 4131 5703 2303
Size: 1828 1572 2988
Fragments arranged by size:
2988 1828 1572
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 2541 3697 3923 4075 6184 1493
Size: 1048 1156 226 152 2109 1697
Fragments arranged by size:
2109 1697 1156 1048 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4942 160
Size: 872 3910 1606
Fragments arranged by size:
3910 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 3234 3713 1870
Size: 73 1291 479 4545
Fragments arranged by size:
4545 1291 479 73
AvrII C'CTAG_G
Cuts at: 3508 3508
Size: 6388
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2571 2604 2571
Size: 33 6355
BamHI G'GATC_C
Cuts at: 2260 2322 3138 3718 4085 2260
Size: 62 816 580 367 4563
Fragments arranged by size:
4563 816 580 367 62
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3709 5164 618
Size: 325 217 627 1922 1455 1842
Fragments arranged by size:
1922 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3161 3707 1391
Size: 1770 546 4072
Fragments arranged by size:
4072 1770 546
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3280 928
Size: 971 1381 4036
Fragments arranged by size:
4036 1381 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2852 2886 4579 4613 1903
Size: 34 915 34 1693 34 3678
Fragments arranged by size:
3678 1693 915 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2436 4280 5490 2436
Size: 1844 1210 3334
Fragments arranged by size:
3334 1844 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6388
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4999 136
Size: 107 122 71 631 605 3327 1525
Fragments arranged by size:
3327 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2166 6383 2166
Size: 4217 2171
BlpI GC'TnA_GC
Cuts at: 2506 3805 2506
Size: 1299 5089
Bme1580I G_kGCm'C
Cuts at: 1790 4449 5605 1790
Size: 2659 1156 2573
Fragments arranged by size:
2659 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2387 2400 3611
Size: 416 53 83 399 24 970 13 1211
Cuts at: 3611 4119 5077 442
Size: 508 958 1753
Fragments arranged by size:
1753 1211 970 958 508 416 399 83
53 24 13
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2185 2206 2185
Size: 21 6367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2070 2313 2342 5048 1239
Size: 831 243 29 2706 2579
Fragments arranged by size:
2706 2579 831 243 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2699 4513 5378 5676 2699
Size: 1814 865 298 3411
Fragments arranged by size:
3411 1814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5051 882
Size: 617 3552 2219
Fragments arranged by size:
3552 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4179 493
Size: 711 2975 2702
Fragments arranged by size:
2975 2702 711
BsaBI GATnn'nnATC
Cuts at: 6382 6382
Size: 6388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4195 4577 5212 275
Size: 53 83 186 936 2662 382 635 1451
Fragments arranged by size:
2662 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2225 2850 4821 5219 5493 5640 6051 2225
Size: 625 1971 398 274 147 411 2562
Fragments arranged by size:
2562 1971 625 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6163
Fragments arranged by size:
6163 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2195 2198 1186
Size: 964 45 3 5376
Fragments arranged by size:
5376 964 45 3
BseYI C'CCAG_C
Cuts at: 2011 2696 3091 5591 6087 2011
Size: 685 395 2500 496 2312
Fragments arranged by size:
2500 2312 685 496 395
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3099 1163
Size: 573 1363 4452
Fragments arranged by size:
4452 1363 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3838 4599 5627 1540
Size: 275 186 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3161 4449 4534 5605 3161
Size: 1288 85 1071 3944
Fragments arranged by size:
3944 1288 1071 85
BsmI GAATG_Cn'
Cuts at: 2818 3899 3992 2818
Size: 1081 93 5214
Fragments arranged by size:
5214 1081 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3161 3707 4449 4534 5605 1391
Size: 399 1371 546 742 85 1071 2174
Fragments arranged by size:
2174 1371 1071 742 546 399 85
BspEI T'CCGG_A
Cuts at: 6051 6051
Size: 6388
BspHI T'CATG_A
Cuts at: 4277 6007 4277
Size: 1730 4658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2823 2980 3743 844
Size: 1979 157 763 3489
Fragments arranged by size:
3489 1979 763 157
BsrBI CCG'CTC
Cuts at: 1394 1823 4275 1394
Size: 429 2452 3507
Fragments arranged by size:
3507 2452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2247 2511 2571 2907 3593 4883 5057
Size: 1183 999 264 60 336 686 1290 174
Cuts at: 5057 65
Size: 1396
Fragments arranged by size:
1396 1290 1183 999 686 336 264 174
60
BsrFI r'CCGG_y
Cuts at: 1700 1810 5032 5219 1700
Size: 110 3222 187 2869
Fragments arranged by size:
3222 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3005 96
Size: 2909 3479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6388
BssSI C'ACGA_G
Cuts at: 2439 4448 5460 2439
Size: 2009 1012 3367
Fragments arranged by size:
3367 2009 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6388
BstBI TT'CG_AA
Cuts at: 5192 5192
Size: 6388
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6388
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2344 2011
Size: 333 6055
BstYI r'GATC_y
Cuts at: 1972 2166 2260 2322 3138 3718 4085 4470 4487
Size: 194 94 62 816 580 367 385 17
Cuts at: 4487 5939 6025 6037 6383 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 816 580 385 367 346 194
94 86 62 17 12
Bsu36I CC'TnA_GG
Cuts at: 2178 2178
Size: 6388
BtgI C'CryG_G
Cuts at: 513 1065 2004 3673 513
Size: 552 939 1669 3228
Fragments arranged by size:
3228 1669 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5649
Fragments arranged by size:
5649 683 56
BtsI GCAGTG_nn'
Cuts at: 2304 3138 3904 4698 4718 2304
Size: 834 766 794 20 3974
Fragments arranged by size:
3974 834 794 766 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3259 3294 529
Size: 35 2695 35 3623
Fragments arranged by size:
3623 2695 35 35
DraI TTT'AAA
Cuts at: 3692 3692
Size: 6388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 3184 3395
Size: 54 1425 50 275 18 504 850 211
Cuts at: 3395 3835 4724 6353 8
Size: 440 889 1629 43
Fragments arranged by size:
1629 1425 889 850 504 440 275 211
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2600 4318 1353
Size: 1247 1718 3423
Fragments arranged by size:
3423 1718 1247
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3448 4975 5349 5495 387
Size: 1064 138 1859 1527 374 146 1280
Fragments arranged by size:
1859 1527 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3159 3159
Size: 6388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2070 2313 2342 2427 2982 4451 5048
Size: 393 438 243 29 85 555 1469 597
Cuts at: 5048 5835 6103 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1469 787 597 555 438 393 268
243 85 29
EcoO109I rG'GnC_Cy
Cuts at: 2015 2434 3505 2015
Size: 419 1071 4898
Fragments arranged by size:
4898 1071 419
EcoRI G'AATT_C
Cuts at: 2541 3697 4075 2541
Size: 1156 378 4854
Fragments arranged by size:
4854 1156 378
FspI TGC'GCA
Cuts at: 4894 4894
Size: 6388
HaeII r_GCGC'y
Cuts at: 1678 5535 1678
Size: 3857 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 2778 2810 5029
Size: 32 135 32 701 32 473 32 2219
Cuts at: 5029 5061 5103 5135 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2219 701 473 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3732 3978 669
Size: 988 281 1794 246 3079
Fragments arranged by size:
3079 1794 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6388
HpaI GTT'AAC
Cuts at: 3978 3978
Size: 6388
KpnI G_GTAC'C
Cuts at: 3713 3713
Size: 6388
MfeI C'AATT_G
Cuts at: 3987 3987
Size: 6388
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5502 5686 1305
Size: 4197 184 2007
Fragments arranged by size:
4197 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2336 3186 10
Size: 54 1425 343 504 850 3212
Fragments arranged by size:
3212 1425 850 504 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2342 2654 3476 4346 4705 4864 518
Size: 648 1176 312 822 870 359 159 2042
Fragments arranged by size:
2042 1176 870 822 648 359 312 159
MspA1I CmG'CkG
Cuts at: 1606 2362 3804 4481 5629 5874 1606
Size: 756 1442 677 1148 245 2120
Fragments arranged by size:
2120 1442 1148 756 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4897
Fragments arranged by size:
4897 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6388
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6388
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 2491 2725 2812 3518 3610 3746 5291
Size: 97 1262 234 87 706 92 136 1545
Cuts at: 5291 1132
Size: 2229
Fragments arranged by size:
2229 1545 1262 706 234 136 97 92
87
PasI CC'CwG_GG
Cuts at: 1348 2123 1348
Size: 775 5613
PciI A'CATG_T
Cuts at: 2808 5287 2808
Size: 2479 3909
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6388
PfoI T'CCnGG_A
Cuts at: 2263 3501 3615 2263
Size: 1238 114 5036
Fragments arranged by size:
5036 1238 114
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4457 4489 5970 6002 4457
Size: 32 1481 32 4843
Fragments arranged by size:
4843 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2015 2434 3505 2015
Size: 419 1071 4898
Fragments arranged by size:
4898 1071 419
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3958 3958
Size: 6388
PstI C_TGCA'G
Cuts at: 830 3151 3740 830
Size: 2321 589 3478
Fragments arranged by size:
3478 2321 589
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
SacI G_AGCT'C
Cuts at: 3161 3161
Size: 6388
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SbfI CC_TGCA'GG
Cuts at: 3740 3740
Size: 6388
ScaI AGT'ACT
Cuts at: 2141 2189 2888 4636 6348 2141
Size: 48 699 1748 1712 2181
Fragments arranged by size:
2181 1748 1712 699 48
SexAI A'CCwGG_T
Cuts at: 2230 2230
Size: 6388
SfcI C'TryA_G
Cuts at: 826 1045 2095 2532 3147 3338 3736 4871 5552
Size: 219 1050 437 615 191 398 1135 681
Cuts at: 5552 5743 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1050 681 615 437 398 219
191 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 3715 1872
Size: 1843 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2714 4492 5393 5655 820
Size: 197 926 771 1778 901 262 1553
Fragments arranged by size:
1778 1553 926 901 771 262 197
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 3746 1132
Size: 2614 3774
SspI AAT'ATT
Cuts at: 5 52 2272 4312 5
Size: 47 2220 2040 2081
Fragments arranged by size:
2220 2081 2040 47
StuI AGG'CCT
Cuts at: 1517 3651 1517
Size: 2134 4254
StyI C'CwwG_G
Cuts at: 513 1065 2004 2430 3508 3827 513
Size: 552 939 426 1078 319 3074
Fragments arranged by size:
3074 1078 939 552 426 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4415 4432 4585 4770 5215 325
Size: 1255 2835 17 153 185 445 1498
Fragments arranged by size:
2835 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2139 2187 2886
Size: 274 80 33 51 1443 162 48 699
Cuts at: 2886 3005 3480 4634 6346 96
Size: 119 475 1154 1712 138
Fragments arranged by size:
1712 1443 1154 699 475 274 162 138
119 80 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 6348 6380 2430
Size: 32 3886 32 2438
Fragments arranged by size:
3886 2438 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6388
XbaI T'CTAG_A
Cuts at: 2837 3724 2837
Size: 887 5501
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2989 2989
Size: 6388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 3713 1870
Size: 1843 4545
XmnI GAAnn'nnTTC
Cuts at: 3178 4517 3178
Size: 1339 5049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI