Restriction map for
pFN21AB3651
(Circular) (Six-base) MAPSORT of: pFN21AB3651.seq Check: 7013 from: 1 to: 7717
pFN21AB3651 7717 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3088 3088
Size: 7717
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5527 278
Size: 53 83 186 936 3991 2468
Fragments arranged by size:
3991 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5060 1937
Size: 3123 4594
Acc65I G'GTAC_C
Cuts at: 5038 5038
Size: 7717
AclI AA'CG_TT
Cuts at: 1563 5844 6217 1563
Size: 4281 373 3063
Fragments arranged by size:
4281 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3419 4104 4298 4418 4535 5780 7164 7432
Size: 1787 685 194 120 117 1245 1384 268
Cuts at: 7432 1632
Size: 1917
Fragments arranged by size:
1917 1787 1384 1245 685 268 194 120
117
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7520
AflIII A'CryG_T
Cuts at: 1203 3811 6616 7593 1203
Size: 2608 2805 977 1327
Fragments arranged by size:
2805 2608 1327 977
AgeI A'CCGG_T
Cuts at: 6548 6548
Size: 7717
AhdI GACnn_n'nnGTC
Cuts at: 6446 6446
Size: 7717
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7717
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2163 3216 1184
Size: 979 1053 5685
Fragments arranged by size:
5685 1053 979
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2018 2050 2402 2434 2018
Size: 32 352 32 7301
Fragments arranged by size:
7301 352 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2122 2969 3656 4276 5460 7032 2122
Size: 847 687 620 1184 1572 2807
Fragments arranged by size:
2807 1572 1184 847 687 620
ApaI G_GGCC'C
Cuts at: 4973 4973
Size: 7717
ApaLI G'TGCA_C
Cuts at: 3744 3887 5774 6930 3744
Size: 143 1887 1156 4531
Fragments arranged by size:
4531 1887 1156 143
ApoI r'AATT_y
Cuts at: 1493 4886 5026 5252 5404 7513 1493
Size: 3393 140 226 152 2109 1697
Fragments arranged by size:
3393 2109 1697 226 152 140
AseI AT'TA_AT
Cuts at: 160 1032 6271 160
Size: 872 5239 1606
Fragments arranged by size:
5239 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7717
AvaI C'yCGr_G
Cuts at: 1870 1943 3669 5042 1870
Size: 73 1726 1373 4545
Fragments arranged by size:
4545 1726 1373 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2919 2952 2919
Size: 33 7684
BamHI G'GATC_C
Cuts at: 5047 5414 5047
Size: 367 7350
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2572 5038 6493 618
Size: 325 217 627 785 2466 1455 1842
Fragments arranged by size:
2466 1842 1455 785 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2369 4973 5036 1391
Size: 978 2604 63 4072
Fragments arranged by size:
4072 2604 978 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3432 4647 928
Size: 971 1533 1215 3998
Fragments arranged by size:
3998 1533 1215 971
BbvCI CC'TCA_GC
Cuts at: 2650 3348 2650
Size: 698 7019
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4832 4866 5908 5942 1903
Size: 34 2895 34 1042 34 3678
Fragments arranged by size:
3678 2895 1042 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5609 6819 5609
Size: 1210 6507
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7717
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3017 3710 6328
Size: 107 122 71 631 605 1345 693 2618
Cuts at: 6328 136
Size: 1525
Fragments arranged by size:
2618 1525 1345 693 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4983 7712 4983
Size: 2729 4988
BlpI GC'TnA_GC
Cuts at: 3106 5134 3106
Size: 2028 5689
Bme1580I G_kGCm'C
Cuts at: 1790 3748 3761 3891 4973 5778 6934 1790
Size: 1958 13 130 1082 805 1156 2573
Fragments arranged by size:
2573 1958 1156 1082 805 130 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7717
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2414 3385 5448
Size: 416 53 83 399 24 997 971 2063
Cuts at: 5448 6406 442
Size: 958 1753
Fragments arranged by size:
2063 1753 997 971 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7717
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 7717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 2613 2682 3152 3251 3981 6377 1239
Size: 923 451 69 470 99 730 2396 2579
Fragments arranged by size:
2579 2396 923 730 470 451 99 69
Bpu10I CC'TnA_GC
Cuts at: 2056 2650 3348 3474 2056
Size: 594 698 126 6299
Fragments arranged by size:
6299 698 594 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2640 4964 4991 5842 6707 7005 2640
Size: 2324 27 851 865 298 3352
Fragments arranged by size:
3352 2324 865 851 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5006 6380 882
Size: 617 3507 1374 2219
Fragments arranged by size:
3507 2219 1374 617
BsaAI yAC'GTr
Cuts at: 493 1204 5508 493
Size: 711 4304 2702
Fragments arranged by size:
4304 2702 711
BsaBI GATnn'nnATC
Cuts at: 2610 3203 7711 2610
Size: 593 4508 2616
Fragments arranged by size:
4508 2616 593
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5524 5906 6541 275
Size: 53 83 186 936 3991 382 635 1451
Fragments arranged by size:
3991 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4771 6150 6548 6822 6969 7380 4771
Size: 1379 398 274 147 411 5108
Fragments arranged by size:
5108 1379 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2402 2432 3775 3805 1178
Size: 30 165 30 999 30 1343 30 5090
Fragments arranged by size:
5090 1343 999 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3706 3829 3956 3959 4579 4847 1186
Size: 2520 123 127 3 620 268 4056
Fragments arranged by size:
4056 2520 620 268 127 123 3
BseYI C'CCAG_C
Cuts at: 2199 2337 2467 3086 3240 3396 3558 4721 4814
Size: 138 130 619 154 156 162 1163 93
Cuts at: 4814 4966 6920 7416 2199
Size: 152 1954 496 2500
Fragments arranged by size:
2500 1954 1163 619 496 162 156 154
152 138 130 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 2960 3024 3710 3818 1163
Size: 573 399 825 64 686 108 5062
Fragments arranged by size:
5062 825 686 573 399 108 64
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2334 5167 5928 6956 1540
Size: 275 186 333 2833 761 1028 2301
Fragments arranged by size:
2833 2301 1028 761 333 275 186
BsiHKAI G_wGCw'C
Cuts at: 2369 3748 3891 5778 5863 6934 2369
Size: 1379 143 1887 85 1071 3152
Fragments arranged by size:
3152 1887 1379 1071 143 85
BsmI GAATG_Cn'
Cuts at: 4078 5228 5321 4078
Size: 1150 93 6474
Fragments arranged by size:
6474 1150 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 3748 3761 3891 4973 5036 5778
Size: 399 579 1379 13 130 1082 63 742
Cuts at: 5778 5863 6934 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1379 1082 1071 742 579 399 130
85 63 13
BspEI T'CCGG_A
Cuts at: 7380 7380
Size: 7717
BspHI T'CATG_A
Cuts at: 5606 7336 5606
Size: 1730 5987
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2473 3088 3367 5072 844
Size: 1629 615 279 1705 3489
Fragments arranged by size:
3489 1705 1629 615 279
BsrBI CCG'CTC
Cuts at: 1394 1823 5604 1394
Size: 429 3781 3507
Fragments arranged by size:
3781 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4596 6212 6386 65
Size: 1183 3348 1616 174 1396
Fragments arranged by size:
3348 1616 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3231 6361 6548 1700
Size: 110 1421 3130 187 2869
Fragments arranged by size:
3130 2869 1421 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7717
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7717
BssSI C'ACGA_G
Cuts at: 5777 6789 5777
Size: 1012 6705
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2122 2131 2572 2947 3002 3886 794
Size: 1328 9 441 375 55 884 4625
Fragments arranged by size:
4625 1328 884 441 375 55 9
BstBI TT'CG_AA
Cuts at: 6521 6521
Size: 7717
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7717
BstXI CCAn_nnnn'nTGG
Cuts at: 3740 3740
Size: 7717
BstYI r'GATC_y
Cuts at: 1972 3123 3159 4983 5047 5414 5799 5816 7268
Size: 1151 36 1824 64 367 385 17 1452
Cuts at: 7268 7354 7366 7712 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1824 1452 1151 385 367 346 86
64 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2893 2893
Size: 7717
BtgI C'CryG_G
Cuts at: 513 1065 2004 2838 4143 513
Size: 552 939 834 1305 4087
Fragments arranged by size:
4087 1305 939 834 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6978
Fragments arranged by size:
6978 683 56
BtsI GCAGTG_nn'
Cuts at: 2118 2129 2821 3339 3746 4096 5233 6027 6047
Size: 11 692 518 407 350 1137 794 20
Cuts at: 6047 2118
Size: 3788
Fragments arranged by size:
3788 1137 794 692 518 407 350 20
11
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7717
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7682
DraI TTT'AAA
Cuts at: 5021 5021
Size: 7717
DraIII CAC_nnn'GTG
Cuts at: 2870 2870
Size: 7717
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6724 809
Size: 507 5408 1802
Fragments arranged by size:
5408 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 2331 2459
Size: 54 1425 50 275 18 489 12 128
Cuts at: 2459 3037 5164 6053 7682 8
Size: 578 2127 889 1629 43
Fragments arranged by size:
2127 1629 1425 889 578 489 275 128
54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2331 5164 1537
Size: 275 519 2833 4090
Fragments arranged by size:
4090 2833 519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3459 4025 4511 5647 1353
Size: 2106 566 486 1136 3423
Fragments arranged by size:
3423 2106 1136 566 486
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6304 6678 6824 387
Size: 1064 138 4715 374 146 1280
Fragments arranged by size:
4715 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2367 2367
Size: 7717
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2613 2682 3152 3251 3419 3981
Size: 393 530 451 69 470 99 168 562
Cuts at: 3981 4104 4298 4418 4535 5780 6377 7164 7432
Size: 123 194 120 117 1245 597 787 268
Cuts at: 7432 1239
Size: 1524
Fragments arranged by size:
1524 1245 787 597 562 530 470 451
393 268 194 168 123 120 117 99
69
EcoNI CCTnn'n_nnAGG
Cuts at: 2480 2726 2892 3531 2480
Size: 246 166 639 6666
Fragments arranged by size:
6666 639 246 166
EcoO109I rG'GnC_Cy
Cuts at: 2660 2743 3361 3379 3859 4915 4970 2660
Size: 83 618 18 480 1056 55 5407
Fragments arranged by size:
5407 1056 618 480 83 55 18
EcoRI G'AATT_C
Cuts at: 5026 5404 5026
Size: 378 7339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4958 4958
Size: 7717
FspI TGC'GCA
Cuts at: 6223 6223
Size: 7717
HaeII r_GCGC'y
Cuts at: 1678 6864 1678
Size: 5186 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2561 2593 3775 3807 3942
Size: 32 135 32 989 32 1182 32 135
Cuts at: 3942 3944 3974 3976 4315 4319 4347 4349 4351
Size: 2 30 2 339 4 28 2 2
Cuts at: 4351 4381 6358 6390 6432 6464 1373
Size: 30 1977 32 42 32 2626
Fragments arranged by size:
2626 1977 1182 989 339 135 135 42
32 32 32 32 32 32 30 30
28 4 2 2 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5061 5307 669
Size: 988 281 3123 246 3079
Fragments arranged by size:
3123 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7717
HpaI GTT'AAC
Cuts at: 5307 5307
Size: 7717
KpnI G_GTAC'C
Cuts at: 5042 5042
Size: 7717
MfeI C'AATT_G
Cuts at: 2235 5316 2235
Size: 3081 4636
MluI A'CGCG_T
Cuts at: 7593 7593
Size: 7717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2483 4337 4715 4802 6831 7015 1305
Size: 1178 1854 378 87 2029 184 2007
Fragments arranged by size:
2029 2007 1854 1178 378 184 87
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 3039 10
Size: 54 1425 343 489 718 4688
Fragments arranged by size:
4688 1425 718 489 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 2604 5675 6034 6193 518
Size: 648 1368 70 3071 359 159 2042
Fragments arranged by size:
3071 2042 1368 648 359 159 70
MspA1I CmG'CkG
Cuts at: 1606 2137 2203 2467 3554 3646 3653 5133 5810
Size: 531 66 264 1087 92 7 1480 677
Cuts at: 5810 6958 7203 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1480 1148 1087 677 531 264 245
92 66 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 2838 4143 513
Size: 552 939 834 1305 4087
Fragments arranged by size:
4087 1305 939 834 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7717
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7717
NotI GC'GGCC_GC
Cuts at: 5164 5164
Size: 7717
NspI r_CATG'y
Cuts at: 1132 1229 3815 5075 6620 1132
Size: 97 2586 1260 1545 2229
Fragments arranged by size:
2586 2229 1545 1260 97
PasI CC'CwG_GG
Cuts at: 1348 2497 2831 3776 1348
Size: 1149 334 945 5289
Fragments arranged by size:
5289 1149 945 334
PciI A'CATG_T
Cuts at: 3811 6616 3811
Size: 2805 4912
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3415 3913 4821 1108
Size: 2307 498 908 4004
Fragments arranged by size:
4004 2307 908 498
PfoI T'CCnGG_A
Cuts at: 2590 2698 4233 2590
Size: 108 1535 6074
Fragments arranged by size:
6074 1535 108
PmeI GTTT'AAAC
Cuts at: 5021 5021
Size: 7717
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2402 2434 5786 5818 7299 7331 2402
Size: 32 3352 32 1481 32 2788
Fragments arranged by size:
3352 2788 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2660 3361 3379 3859 2660
Size: 701 18 480 6518
Fragments arranged by size:
6518 701 480 18
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7717
PsiI TTA'TAA
Cuts at: 5287 5287
Size: 7717
PspOMI G'GGCC_C
Cuts at: 4969 4969
Size: 7717
PstI C_TGCA'G
Cuts at: 830 2136 2560 2956 3753 5069 830
Size: 1306 424 396 797 1316 3478
Fragments arranged by size:
3478 1316 1306 797 424 396
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7717
PvuII CAG'CTG
Cuts at: 2203 2467 3554 3646 3653 2203
Size: 264 1087 92 7 6267
Fragments arranged by size:
6267 1087 264 92 7
SacI G_AGCT'C
Cuts at: 2369 2369
Size: 7717
SalI G'TCGA_C
Cuts at: 1936 5059 1936
Size: 3123 4594
SapI GCTCTTCn'nnn_
Cuts at: 4025 4025
Size: 7717
SbfI CC_TGCA'GG
Cuts at: 5069 5069
Size: 7717
ScaI AGT'ACT
Cuts at: 4409 5965 7677 4409
Size: 1556 1712 4449
Fragments arranged by size:
4449 1712 1556
SfcI C'TryA_G
Cuts at: 826 1045 2132 2556 2952 3507 3749 4298 4495
Size: 219 1087 424 396 555 242 549 197
Cuts at: 4495 5065 6200 6881 7072 826
Size: 570 1135 681 191 1471
Fragments arranged by size:
1471 1135 1087 681 570 555 549 424
396 242 219 197 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3710 3710
Size: 7717
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7717
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7717
SmaI CCC'GGG
Cuts at: 1872 3671 5044 1872
Size: 1799 1373 4545
Fragments arranged by size:
4545 1799 1373
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2655 4979 5006 5821 6722 6984
Size: 197 926 712 2324 27 815 901 262
Cuts at: 6984 820
Size: 1553
Fragments arranged by size:
2324 1553 926 901 815 712 262 197
27
SnaBI TAC'GTA
Cuts at: 493 5508 493
Size: 5015 2702
SpeI A'CTAG_T
Cuts at: 152 4096 152
Size: 3944 3773
SphI G_CATG'C
Cuts at: 1132 5075 1132
Size: 3943 3774
SspI AAT'ATT
Cuts at: 5 52 5641 5
Size: 47 5589 2081
Fragments arranged by size:
5589 2081 47
StuI AGG'CCT
Cuts at: 1517 2306 2648 2732 1517
Size: 789 342 84 6502
Fragments arranged by size:
6502 789 342 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 2838 3987 4143 4861 5156 513
Size: 552 939 834 1149 156 718 295 3074
Fragments arranged by size:
3074 1149 939 834 718 552 295 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2315 2891 4151 4223 5744 5761 5914
Size: 1255 735 576 1260 72 1521 17 153
Cuts at: 5914 6099 6544 325
Size: 185 445 1498
Fragments arranged by size:
1521 1498 1260 1255 735 576 445 185
153 72 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4407 5963 7675
Size: 274 80 33 51 1443 2430 1556 1712
Cuts at: 7675 96
Size: 138
Fragments arranged by size:
2430 1712 1556 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3345 3377 3561 3593 7677 7709 3221
Size: 32 92 32 184 32 4084 32 3229
Fragments arranged by size:
4084 3229 184 92 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7717
XbaI T'CTAG_A
Cuts at: 4151 5053 4151
Size: 902 6815
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7717
XmaI C'CCGG_G
Cuts at: 1870 3669 5042 1870
Size: 1799 1373 4545
Fragments arranged by size:
4545 1799 1373
XmnI GAAnn'nnTTC
Cuts at: 5846 5846
Size: 7717
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5525 276
Size: 53 83 186 936 3991 2468
Fragments arranged by size:
3991 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SfoI SrfI SwaI XcmI