Restriction map for
pFN21AB3650
(Circular) (Six-base) MAPSORT of: pFN21AB3650.seq Check: 6361 from: 1 to: 6139
pFN21AB3650 6139 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2817 3482 1937
Size: 880 665 4594
Fragments arranged by size:
4594 880 665
Acc65I G'GTAC_C
Cuts at: 2577 2987 3460 2577
Size: 410 473 5256
Fragments arranged by size:
5256 473 410
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4202 5586 5854 1632
Size: 2570 1384 268 1917
Fragments arranged by size:
2570 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 5038 6015 1203
Size: 3835 977 1327
Fragments arranged by size:
3835 1327 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 2068 4868 2068
Size: 2800 3339
AleI CACnn'nnGTG
Cuts at: 1166 3162 1166
Size: 1996 4143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6139
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 6107
AlwNI CAG_nnn'CTG
Cuts at: 3428 3882 5454 3428
Size: 454 1572 4113
Fragments arranged by size:
4113 1572 454
ApaI G_GGCC'C
Cuts at: 2291 2618 2291
Size: 327 5812
ApaLI G'TGCA_C
Cuts at: 4196 5352 4196
Size: 1156 4983
ApoI r'AATT_y
Cuts at: 1493 3448 3674 3826 5935 1493
Size: 1955 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 3464 1870
Size: 73 1521 4545
Fragments arranged by size:
4545 1521 73
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2387 2577 2987 3082
Size: 325 217 627 221 379 190 410 95
Cuts at: 3082 3460 4915 618
Size: 378 1455 1842
Fragments arranged by size:
1842 1455 627 410 379 378 325 221
217 190 95
BanII G_rGCy'C
Cuts at: 1391 2133 2291 2618 3458 1391
Size: 742 158 327 840 4072
Fragments arranged by size:
4072 840 742 327 158
BbeI G_GCGC'C
Cuts at: 2391 2391
Size: 6139
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2663 3304 928
Size: 971 764 641 3763
Fragments arranged by size:
3763 971 764 641
BbvCI CC'TCA_GC
Cuts at: 2445 2445
Size: 6139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4330 4364 1903
Size: 34 2393 34 3678
Fragments arranged by size:
3678 2393 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 4750 136
Size: 107 122 71 631 605 370 2708 1525
Fragments arranged by size:
2708 1525 631 605 370 122 107 71
BglII A'GATC_T
Cuts at: 6134 6134
Size: 6139
BlpI GC'TnA_GC
Cuts at: 3556 3556
Size: 6139
Bme1580I G_kGCm'C
Cuts at: 1790 2291 2618 4200 5356 1790
Size: 501 327 1582 1156 2573
Fragments arranged by size:
2573 1582 1156 501 327
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2311 2851 2862
Size: 416 53 83 399 24 894 540 11
Cuts at: 2862 2995 3870 4828 442
Size: 133 875 958 1753
Fragments arranged by size:
1753 958 894 875 540 416 399 133
83 53 24 11
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 3205 3345 4799 1239
Size: 1055 911 140 1454 2579
Fragments arranged by size:
2579 1454 1055 911 140
Bpu10I CC'TnA_GC
Cuts at: 2334 2445 2334
Size: 111 6028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2398 2489 4264 5129 5427 2398
Size: 91 1775 865 298 3110
Fragments arranged by size:
3110 1775 865 298 91
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2011 2417 2434 4802 882
Size: 617 512 406 17 2368 2219
Fragments arranged by size:
2368 2219 617 512 406 17
BsaAI yAC'GTr
Cuts at: 493 1204 3204 3930 493
Size: 711 2000 726 2702
Fragments arranged by size:
2702 2000 726 711
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2388 2490 3946 4328
Size: 53 83 186 936 855 102 1456 382
Cuts at: 4328 4963 275
Size: 635 1451
Fragments arranged by size:
1456 1451 936 855 635 382 186 102
83 53
BsaWI w'CCGG_w
Cuts at: 4572 4970 5244 5391 5802 4572
Size: 398 274 147 411 4909
Fragments arranged by size:
4909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2210 2240 2453 2483 2837
Size: 30 165 30 807 30 213 30 354
Cuts at: 2837 2867 3164 3194 3377 3407 1178
Size: 30 297 30 183 30 3910
Fragments arranged by size:
3910 807 354 297 213 183 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2264 2474 2477 2891 3242 1186
Size: 1078 210 3 414 351 4083
Fragments arranged by size:
4083 1078 414 351 210 3
BseYI C'CCAG_C
Cuts at: 2452 2940 3412 3421 5342 5838 2452
Size: 488 472 9 1921 496 2753
Fragments arranged by size:
2753 1921 496 488 472 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2071 2140 2491 1163
Size: 573 335 69 351 4811
Fragments arranged by size:
4811 573 351 335 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3589 4350 5378 1540
Size: 275 186 1588 761 1028 2301
Fragments arranged by size:
2301 1588 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 4200 4285 5356 2133
Size: 2067 85 1071 2916
Fragments arranged by size:
2916 2067 1071 85
BsmI GAATG_Cn'
Cuts at: 3650 3743 3650
Size: 93 6046
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2291 2618 3458 4200 4285 5356
Size: 399 343 158 327 840 742 85 1071
Cuts at: 5356 1391
Size: 2174
Fragments arranged by size:
2174 1071 840 742 399 343 327 158
85
BspEI T'CCGG_A
Cuts at: 5802 5802
Size: 6139
BspHI T'CATG_A
Cuts at: 4028 5758 4028
Size: 1730 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3494 844
Size: 2650 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2812 4026 1394
Size: 429 989 1214 3507
Fragments arranged by size:
3507 1214 989 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4634 4808 65
Size: 1183 3386 174 1396
Fragments arranged by size:
3386 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3186 4783 4970 1700
Size: 110 1376 1597 187 2869
Fragments arranged by size:
2869 1597 1376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6139
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 4199 5211 4199
Size: 1012 5127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2051 3028 794
Size: 1257 977 3905
Fragments arranged by size:
3905 1257 977
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6139
BstXI CCAn_nnnn'nTGG
Cuts at: 3164 3164
Size: 6139
BstYI r'GATC_y
Cuts at: 1972 3469 3836 4221 4238 5690 5776 5788 6134
Size: 1497 367 385 17 1452 86 12 346
Cuts at: 6134 1972
Size: 1977
Fragments arranged by size:
1977 1497 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2358 2379 2400 2358
Size: 21 21 6097
Fragments arranged by size:
6097 21 21
BtgI C'CryG_G
Cuts at: 513 1065 2004 3051 3163 513
Size: 552 939 1047 112 3489
Fragments arranged by size:
3489 1047 939 552 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3107 3382 487
Size: 683 56 1881 275 3244
Fragments arranged by size:
3244 1881 683 275 56
BtsI GCAGTG_nn'
Cuts at: 2479 2571 3655 4449 4469 2479
Size: 92 1084 794 20 4149
Fragments arranged by size:
4149 1084 794 92 20
ClaI AT'CG_AT
Cuts at: 1554 2622 1554
Size: 1068 5071
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DraIII CAC_nnn'GTG
Cuts at: 3028 3028
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2803 3058 3099
Size: 54 1425 50 275 18 973 255 41
Cuts at: 3099 3166 3188 3430 3586 4475 6104 8
Size: 67 22 242 156 889 1629 43
Fragments arranged by size:
1629 1425 973 889 275 255 242 156
67 54 50 43 41 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 3586 1537
Size: 275 1774 4090
Fragments arranged by size:
4090 1774 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2358 2672 2969 4069 1353
Size: 1005 314 297 1100 3423
Fragments arranged by size:
3423 1100 1005 314 297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4726 5100 5246 387
Size: 1064 138 3137 374 146 1280
Fragments arranged by size:
3137 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2131 2131
Size: 6139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 3205 3345 4202 4799 5586 5854
Size: 393 662 911 140 857 597 787 268
Cuts at: 5854 1239
Size: 1524
Fragments arranged by size:
1524 911 857 787 662 597 393 268
140
EcoNI CCTnn'n_nnAGG
Cuts at: 2669 2669
Size: 6139
EcoO109I rG'GnC_Cy
Cuts at: 2287 2288 2368 2614 2615 2287
Size: 1 80 246 1 5811
Fragments arranged by size:
5811 246 80 1 1
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 2391 2692 5286 1678
Size: 713 301 2594 2531
Fragments arranged by size:
2594 2531 713 301
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2010 2042 2054 2086 2416
Size: 32 135 32 438 32 12 32 330
Cuts at: 2416 2448 4780 4812 4854 4886 1373
Size: 32 2332 32 42 32 2626
Fragments arranged by size:
2626 2332 438 330 135 42 32 32
32 32 32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3483 3729 669
Size: 988 281 1545 246 3079
Fragments arranged by size:
3079 1545 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6139
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KasI G'GCGC_C
Cuts at: 2387 2387
Size: 6139
KpnI G_GTAC'C
Cuts at: 2581 2991 3464 2581
Size: 410 473 5256
Fragments arranged by size:
5256 473 410
MfeI C'AATT_G
Cuts at: 3738 3738
Size: 6139
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5253 5437 1305
Size: 3948 184 2007
Fragments arranged by size:
3948 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3101 3432 10
Size: 54 1425 343 1269 331 2717
Fragments arranged by size:
2717 1425 1269 343 331 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3162 4097 4456 4615 518
Size: 648 1996 935 359 159 2042
Fragments arranged by size:
2042 1996 935 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2932 2940 3555 4232 5380 5625 1606
Size: 479 847 8 615 677 1148 245 2120
Fragments arranged by size:
2120 1148 847 677 615 479 245 8
NaeI GCC'GGC
Cuts at: 3188 3188
Size: 6139
NarI GG'CG_CC
Cuts at: 2388 2388
Size: 6139
NcoI C'CATG_G
Cuts at: 513 1065 2004 3051 513
Size: 552 939 1047 3601
Fragments arranged by size:
3601 1047 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NgoMIV G'CCGG_C
Cuts at: 3186 3186
Size: 6139
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 3497 5042 1132
Size: 97 2268 1545 2229
Fragments arranged by size:
2268 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 5038 5038
Size: 6139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3428 1108
Size: 2320 3819
PfoI T'CCnGG_A
Cuts at: 2706 2754 2706
Size: 48 6091
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PmlI CAC'GTG
Cuts at: 3204 3204
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2837 2869 4208 4240 5721 5753 2837
Size: 32 1339 32 1481 32 3223
Fragments arranged by size:
3223 1481 1339 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PspOMI G'GGCC_C
Cuts at: 2287 2614 2287
Size: 327 5812
PstI C_TGCA'G
Cuts at: 830 3491 830
Size: 2661 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
PvuII CAG'CTG
Cuts at: 2940 2940
Size: 6139
SacI G_AGCT'C
Cuts at: 2133 2133
Size: 6139
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 2540 2684 2840 3335 3407 4387 6099 2540
Size: 144 156 495 72 980 1712 2580
Fragments arranged by size:
2580 1712 980 495 156 144 72
SfcI C'TryA_G
Cuts at: 826 1045 2797 3262 3487 4622 5303 5494 826
Size: 219 1752 465 225 1135 681 191 1471
Fragments arranged by size:
1752 1471 1135 681 465 225 219 191
SfoI GGC'GCC
Cuts at: 2389 2389
Size: 6139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 3466 1872
Size: 1594 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2413 2504 4243 5144 5406 820
Size: 197 926 470 91 1739 901 262 1553
Fragments arranged by size:
1739 1553 926 901 470 262 197 91
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 2419 2572 1517
Size: 902 153 5084
Fragments arranged by size:
5084 902 153
StyI C'CwwG_G
Cuts at: 513 1065 2004 2775 3051 3096 3578 513
Size: 552 939 771 276 45 482 3074
Fragments arranged by size:
3074 939 771 552 482 276 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4166 4183 4336 4521 4966 325
Size: 1255 2586 17 153 185 445 1498
Fragments arranged by size:
2586 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2538 2682
Size: 274 80 33 51 1443 249 312 144
Cuts at: 2682 2838 3333 3405 4385 6097 96
Size: 156 495 72 980 1712 138
Fragments arranged by size:
1712 1443 980 495 312 274 249 156
144 138 80 72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2099 2131 2453 2485 6099 6131 2099
Size: 32 322 32 3614 32 2107
Fragments arranged by size:
3614 2107 322 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6139
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2798 2798
Size: 6139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 3464 1870
Size: 1594 4545
XmnI GAAnn'nnTTC
Cuts at: 2483 4268 2483
Size: 1785 4354
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI