Restriction map for
pFN21AB3649
(Circular) (Six-base) MAPSORT of: pFN21AB3649.seq Check: 609 from: 1 to: 6481
pFN21AB3649 6481 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3824 1937
Size: 1887 4594
Acc65I G'GTAC_C
Cuts at: 3802 3802
Size: 6481
AclI AA'CG_TT
Cuts at: 1563 4608 4981 1563
Size: 3045 373 3063
Fragments arranged by size:
3063 3045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2702 3395 3456 3693 4544 5928 6196 1632
Size: 1070 693 61 237 851 1384 268 1917
Fragments arranged by size:
1917 1384 1070 851 693 268 237 61
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6284
AflIII A'CryG_T
Cuts at: 1203 5380 6357 1203
Size: 4177 977 1327
Fragments arranged by size:
4177 1327 977
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 5210 5210
Size: 6481
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6481
AlwNI CAG_nnn'CTG
Cuts at: 2897 3095 4224 5796 2897
Size: 198 1129 1572 3582
Fragments arranged by size:
3582 1572 1129 198
ApaLI G'TGCA_C
Cuts at: 4538 5694 4538
Size: 1156 5325
ApoI r'AATT_y
Cuts at: 1493 3738 3790 4016 4168 6277 1493
Size: 2245 52 226 152 2109 1697
Fragments arranged by size:
2245 2109 1697 226 152 52
AseI AT'TA_AT
Cuts at: 160 1032 5035 160
Size: 872 4003 1606
Fragments arranged by size:
4003 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 2376 2855 3806 1870
Size: 73 433 479 951 4545
Fragments arranged by size:
4545 951 479 433 73
BamHI G'GATC_C
Cuts at: 3811 4178 3811
Size: 367 6114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2489 3802 5257 618
Size: 325 217 627 702 1313 1455 1842
Fragments arranged by size:
1842 1455 1313 702 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3800 1391
Size: 2409 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 2690 2850 3042 3714 928
Size: 971 240 551 160 192 672 3695
Fragments arranged by size:
3695 971 672 551 240 192 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4672 4706 1903
Size: 34 2735 34 3678
Fragments arranged by size:
3678 2735 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4373 5583 4373
Size: 1210 5271
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6481
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5092 136
Size: 107 122 71 631 605 3420 1525
Fragments arranged by size:
3420 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2677 3232 6476 2677
Size: 555 3244 2682
Fragments arranged by size:
3244 2682 555
BlpI GC'TnA_GC
Cuts at: 3399 3898 3399
Size: 499 5982
Bme1580I G_kGCm'C
Cuts at: 1790 4542 5698 1790
Size: 2752 1156 2573
Fragments arranged by size:
2752 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6481
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2923 3171 4212
Size: 416 53 83 399 24 1506 248 1041
Cuts at: 4212 5170 442
Size: 958 1753
Fragments arranged by size:
1753 1506 1041 958 416 399 248 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2175 5141 1239
Size: 936 2966 2579
Fragments arranged by size:
2966 2579 936
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4606 5471 5769 4606
Size: 865 298 5318
Fragments arranged by size:
5318 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5144 882
Size: 617 3645 2219
Fragments arranged by size:
3645 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2825 2995 4272 493
Size: 711 1621 170 1277 2702
Fragments arranged by size:
2702 1621 1277 711 170
BsaBI GATnn'nnATC
Cuts at: 2676 6475 2676
Size: 3799 2682
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4288 4670 5305 275
Size: 53 83 186 936 2755 382 635 1451
Fragments arranged by size:
2755 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2847 3568 4914 5312 5586 5733 6144 2847
Size: 721 1346 398 274 147 411 3184
Fragments arranged by size:
3184 1346 721 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2140 2143 2170 2173 3269
Size: 30 165 30 737 3 27 3 1096
Cuts at: 3269 3299 1178
Size: 30 4360
Fragments arranged by size:
4360 1096 737 165 30 30 30 27
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2148 2796 3051 3638 3641 3716 3743 1186
Size: 962 648 255 587 3 75 27 3924
Fragments arranged by size:
3924 962 648 587 255 75 27 3
BseYI C'CCAG_C
Cuts at: 5684 6180 5684
Size: 496 5985
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2588 1163
Size: 573 852 5056
Fragments arranged by size:
5056 852 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3931 4692 5720 1540
Size: 275 186 1930 761 1028 2301
Fragments arranged by size:
2301 1930 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4542 4627 5698 4542
Size: 85 1071 5325
Fragments arranged by size:
5325 1071 85
BsmI GAATG_Cn'
Cuts at: 3992 4085 3992
Size: 93 6388
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3800 4542 4627 5698 1391
Size: 399 2010 742 85 1071 2174
Fragments arranged by size:
2174 2010 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6144 6144
Size: 6481
BspHI T'CATG_A
Cuts at: 2065 4370 6100 2065
Size: 2305 1730 2446
Fragments arranged by size:
2446 2305 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3836 844
Size: 2992 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4368 1394
Size: 429 2545 3507
Fragments arranged by size:
3507 2545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4976 5150 65
Size: 1183 3728 174 1396
Fragments arranged by size:
3728 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5125 5312 1700
Size: 110 3315 187 2869
Fragments arranged by size:
3315 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6481
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 4541 5553 4541
Size: 1012 5469
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6481
BstBI TT'CG_AA
Cuts at: 5285 5285
Size: 6481
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6481
BstXI CCAn_nnnn'nTGG
Cuts at: 2913 3538 2913
Size: 625 5856
BstYI r'GATC_y
Cuts at: 1972 2677 2887 3232 3811 4178 4563 4580 6032
Size: 705 210 345 579 367 385 17 1452
Cuts at: 6032 6118 6130 6476 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 705 579 385 367 346 345
210 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3021 3021
Size: 6481
BtgI C'CryG_G
Cuts at: 513 1065 2004 3446 513
Size: 552 939 1442 3548
Fragments arranged by size:
3548 1442 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5742
Fragments arranged by size:
5742 683 56
BtsI GCAGTG_nn'
Cuts at: 2151 3997 4791 4811 2151
Size: 1846 794 20 3821
Fragments arranged by size:
3821 1846 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6481
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6446
DraI TTT'AAA
Cuts at: 3785 3785
Size: 6481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5488 809
Size: 507 4172 1802
Fragments arranged by size:
4172 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3928 4817 6446
Size: 54 1425 50 275 18 2098 889 1629
Cuts at: 6446 8
Size: 43
Fragments arranged by size:
2098 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3928 1537
Size: 275 2116 4090
Fragments arranged by size:
4090 2116 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2943 3432 3509 3576 3585 3594 3633
Size: 765 825 489 77 67 9 9 39
Cuts at: 3633 3645 3769 4411 1353
Size: 12 124 642 3423
Fragments arranged by size:
3423 825 765 642 489 124 77 67
39 12 9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5068 5442 5588 387
Size: 1064 138 3479 374 146 1280
Fragments arranged by size:
3479 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2175 2702 3395 3456 3693 4544 5141
Size: 393 543 527 693 61 237 851 597
Cuts at: 5141 5928 6196 1239
Size: 787 268 1524
Fragments arranged by size:
1524 851 787 693 597 543 527 393
268 237 61
EcoO109I rG'GnC_Cy
Cuts at: 2836 3370 2836
Size: 534 5947
EcoRI G'AATT_C
Cuts at: 3790 4168 3790
Size: 378 6103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3662 3662
Size: 6481
FspI TGC'GCA
Cuts at: 4987 4987
Size: 6481
HaeII r_GCGC'y
Cuts at: 1678 3445 5628 1678
Size: 1767 2183 2531
Fragments arranged by size:
2531 2183 1767
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2865 2897 3090 3122 3157
Size: 32 135 32 1293 32 193 32 35
Cuts at: 3157 3189 3269 3301 5122 5154 5196 5228 1373
Size: 32 80 32 1821 32 42 32 2626
Fragments arranged by size:
2626 1821 1293 193 135 80 42 35
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2761 3825 4071 669
Size: 988 281 823 1064 246 3079
Fragments arranged by size:
3079 1064 988 823 281 246
HindIII A'AGCT_T
Cuts at: 748 2342 748
Size: 1594 4887
HpaI GTT'AAC
Cuts at: 4071 4071
Size: 6481
KpnI G_GTAC'C
Cuts at: 3806 3806
Size: 6481
MfeI C'AATT_G
Cuts at: 4080 4080
Size: 6481
MluI A'CGCG_T
Cuts at: 6357 6357
Size: 6481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2222 2641 5595 5779 1305
Size: 917 419 2954 184 2007
Fragments arranged by size:
2954 2007 917 419 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4659
Fragments arranged by size:
4659 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 4439 4798 4957 518
Size: 648 913 2360 359 159 2042
Fragments arranged by size:
2360 2042 913 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3434 3897 4574 5722 5967 1606
Size: 1828 463 677 1148 245 2120
Fragments arranged by size:
2120 1828 1148 677 463 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4990
Fragments arranged by size:
4990 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 3928 3928
Size: 6481
NspI r_CATG'y
Cuts at: 1132 1229 2660 3839 5384 1132
Size: 97 1431 1179 1545 2229
Fragments arranged by size:
2229 1545 1431 1179 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6481
PciI A'CATG_T
Cuts at: 5380 5380
Size: 6481
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2770 1108
Size: 1662 4819
PfoI T'CCnGG_A
Cuts at: 3094 3094
Size: 6481
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PmlI CAC'GTG
Cuts at: 2825 2825
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4550 4582 6063 6095 4550
Size: 32 1481 32 4936
Fragments arranged by size:
4936 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2836 2836
Size: 6481
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6481
PsiI TTA'TAA
Cuts at: 3141 4051 3141
Size: 910 5571
PstI C_TGCA'G
Cuts at: 830 3833 830
Size: 3003 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6481
PvuII CAG'CTG
Cuts at: 3434 3434
Size: 6481
SalI G'TCGA_C
Cuts at: 1936 3823 1936
Size: 1887 4594
SapI GCTCTTCn'nnn_
Cuts at: 3432 3432
Size: 6481
SbfI CC_TGCA'GG
Cuts at: 3833 3833
Size: 6481
ScaI AGT'ACT
Cuts at: 4729 6441 4729
Size: 1712 4769
SfcI C'TryA_G
Cuts at: 826 1045 3474 3829 4964 5645 5836 826
Size: 219 2429 355 1135 681 191 1471
Fragments arranged by size:
2429 1471 1135 681 355 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6481
SmaI CCC'GGG
Cuts at: 1872 3808 1872
Size: 1936 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4585 5486 5748 820
Size: 197 926 2642 901 262 1553
Fragments arranged by size:
2642 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2995 4272 493
Size: 2502 1277 2702
Fragments arranged by size:
2702 2502 1277
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6481
SphI G_CATG'C
Cuts at: 1132 3839 1132
Size: 2707 3774
SspI AAT'ATT
Cuts at: 5 52 4405 5
Size: 47 4353 2081
Fragments arranged by size:
4353 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6481
StyI C'CwwG_G
Cuts at: 513 1065 2004 2172 3920 513
Size: 552 939 168 1748 3074
Fragments arranged by size:
3074 1748 939 552 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4508 4525 4678 4863 5308 325
Size: 1255 2928 17 153 185 445 1498
Fragments arranged by size:
2928 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4727 6439 96
Size: 274 80 33 51 1443 2750 1712 138
Fragments arranged by size:
2750 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2143 2175 6441 6473 2000
Size: 32 111 32 4266 32 2008
Fragments arranged by size:
4266 2008 111 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2114 1316
Size: 798 5683
XbaI T'CTAG_A
Cuts at: 3817 3817
Size: 6481
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6481
XmaI C'CCGG_G
Cuts at: 1870 3806 1870
Size: 1936 4545
XmnI GAAnn'nnTTC
Cuts at: 4610 4610
Size: 6481
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI