Restriction map for
pFN21AB3647
(Circular) (Six-base) MAPSORT of: pFN21AB3647.seq Check: 6851 from: 1 to: 6499
pFN21AB3647 6499 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2559 2559
Size: 6499
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3435 4309 278
Size: 53 83 186 936 1899 874 2468
Fragments arranged by size:
2468 1899 936 874 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3051 3842 1937
Size: 1114 791 4594
Fragments arranged by size:
4594 1114 791
Acc65I G'GTAC_C
Cuts at: 3348 3820 3348
Size: 472 6027
AclI AA'CG_TT
Cuts at: 1563 4626 4999 1563
Size: 3063 373 3063
Fragments arranged by size:
3063 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2216 2343 2576 2621 4562 5946 6214 1632
Size: 584 127 233 45 1941 1384 268 1917
Fragments arranged by size:
1941 1917 1384 584 268 233 127 45
AfeI AGC'GCT
Cuts at: 3656 3656
Size: 6499
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6302
AflIII A'CryG_T
Cuts at: 1203 5398 6375 1203
Size: 4195 977 1327
Fragments arranged by size:
4195 1327 977
AgeI A'CCGG_T
Cuts at: 5330 5330
Size: 6499
AhdI GACnn_n'nnGTC
Cuts at: 5228 5228
Size: 6499
AleI CACnn'nnGTG
Cuts at: 1166 3540 1166
Size: 2374 4125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3558 1184
Size: 2374 4125
AlwNI CAG_nnn'CTG
Cuts at: 3619 4242 5814 3619
Size: 623 1572 4304
Fragments arranged by size:
4304 1572 623
ApaLI G'TGCA_C
Cuts at: 2519 4556 5712 2519
Size: 2037 1156 3306
Fragments arranged by size:
3306 2037 1156
ApoI r'AATT_y
Cuts at: 1493 2385 2753 3337 3808 4034 4186 6295 1493
Size: 892 368 584 471 226 152 2109 1697
Fragments arranged by size:
2109 1697 892 584 471 368 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5053 160
Size: 872 4021 1606
Fragments arranged by size:
4021 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
AvaI C'yCGr_G
Cuts at: 1870 1943 2639 3824 1870
Size: 73 696 1185 4545
Fragments arranged by size:
4545 1185 696 73
BamHI G'GATC_C
Cuts at: 3034 3829 4196 3034
Size: 795 367 5337
Fragments arranged by size:
5337 795 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3348 3542 3820 5275 618
Size: 325 217 627 1561 194 278 1455 1842
Fragments arranged by size:
1842 1561 1455 627 325 278 217 194
BanII G_rGCy'C
Cuts at: 1391 2265 2439 3818 1391
Size: 874 174 1379 4072
Fragments arranged by size:
4072 1379 874 174
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3020 928
Size: 971 1121 4407
Fragments arranged by size:
4407 1121 971
BbvCI CC'TCA_GC
Cuts at: 2156 3210 2156
Size: 1054 5445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4690 4724 1903
Size: 34 2753 34 3678
Fragments arranged by size:
3678 2753 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3489 4391 5601 3489
Size: 902 1210 4387
Fragments arranged by size:
4387 1210 902
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6499
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 3735 5110
Size: 107 122 71 631 605 475 1588 1375
Cuts at: 5110 136
Size: 1525
Fragments arranged by size:
1588 1525 1375 631 605 475 122 107
71
BglII A'GATC_T
Cuts at: 6494 6494
Size: 6499
BlpI GC'TnA_GC
Cuts at: 2772 3916 2772
Size: 1144 5355
Bme1580I G_kGCm'C
Cuts at: 1790 2523 3547 3698 4560 5716 1790
Size: 733 1024 151 862 1156 2573
Fragments arranged by size:
2573 1156 1024 862 733 151
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6499
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3083 4230 5188
Size: 416 53 83 399 24 1666 1147 958
Cuts at: 5188 442
Size: 1753
Fragments arranged by size:
1753 1666 1147 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6499
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3213 3213
Size: 6499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 3287 3476 3731 5159 1239
Size: 995 1053 189 255 1428 2579
Fragments arranged by size:
2579 1428 1053 995 255 189
Bpu10I CC'TnA_GC
Cuts at: 2156 2337 3210 2156
Size: 181 873 5445
Fragments arranged by size:
5445 873 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2423 4624 5489 5787 2423
Size: 2201 865 298 3135
Fragments arranged by size:
3135 2201 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2940 5162 882
Size: 617 1441 2222 2219
Fragments arranged by size:
2222 2219 1441 617
BsaAI yAC'GTr
Cuts at: 493 1204 2749 4290 493
Size: 711 1545 1541 2702
Fragments arranged by size:
2702 1545 1541 711
BsaBI GATnn'nnATC
Cuts at: 2468 6493 2468
Size: 4025 2474
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 2940 3432 4306
Size: 53 83 186 936 582 825 492 874
Cuts at: 4306 4688 5323 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 874 825 635 582 492 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2407 2802 4932 5330 5604 5751 6162 2407
Size: 395 2130 398 274 147 411 2744
Fragments arranged by size:
2744 2130 411 398 395 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2426 2456 2888 2918 1178
Size: 30 165 30 1023 30 432 30 4759
Fragments arranged by size:
4759 1023 432 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2609 2630 3072 3554 1186
Size: 1423 21 442 482 4131
Fragments arranged by size:
4131 1423 482 442 21
BseYI C'CCAG_C
Cuts at: 2766 2892 2971 3492 3612 5702 6198 2766
Size: 126 79 521 120 2090 496 3067
Fragments arranged by size:
3067 2090 521 496 126 120 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2879 3518 3745 1163
Size: 573 1143 639 227 3917
Fragments arranged by size:
3917 1143 639 573 227
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2135 3949 4710 5738 1540
Size: 275 186 134 1814 761 1028 2301
Fragments arranged by size:
2301 1814 1028 761 275 186 134
BsiHKAI G_wGCw'C
Cuts at: 2073 2439 2523 4560 4645 5716 2073
Size: 366 84 2037 85 1071 2856
Fragments arranged by size:
2856 2037 1071 366 85 84
BsmI GAATG_Cn'
Cuts at: 3199 4010 4103 3199
Size: 811 93 5595
Fragments arranged by size:
5595 811 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2106 2106
Size: 6499
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2265 2439 2523 3547 3698 3818
Size: 399 283 192 174 84 1024 151 120
Cuts at: 3818 4560 4645 5716 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1024 742 399 283 192 174
151 120 85 84
BspEI T'CCGG_A
Cuts at: 2407 6162 2407
Size: 3755 2744
BspHI T'CATG_A
Cuts at: 3684 4388 6118 3684
Size: 704 1730 4065
Fragments arranged by size:
4065 1730 704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2463 2559 2713 3267 3854 844
Size: 1619 96 154 554 587 3489
Fragments arranged by size:
3489 1619 587 554 154 96
BsrBI CCG'CTC
Cuts at: 1394 1823 3145 4386 1394
Size: 429 1322 1241 3507
Fragments arranged by size:
3507 1322 1241 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4994 5168 65
Size: 1183 3746 174 1396
Fragments arranged by size:
3746 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2088 2229 2776 5143 5330 1700
Size: 110 250 28 141 547 2367 187 2869
Fragments arranged by size:
2869 2367 547 250 187 141 110 28
BsrGI T'GTAC_A
Cuts at: 96 2910 3243 3648 96
Size: 2814 333 405 2947
Fragments arranged by size:
2947 2814 405 333
BssHII G'CGCG_C
Cuts at: 1925 2137 2160 1925
Size: 212 23 6264
Fragments arranged by size:
6264 212 23
BssSI C'ACGA_G
Cuts at: 2123 4559 5571 2123
Size: 2436 1012 3051
Fragments arranged by size:
3051 2436 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6499
BstBI TT'CG_AA
Cuts at: 5303 5303
Size: 6499
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6499
BstXI CCAn_nnnn'nTGG
Cuts at: 2931 2931
Size: 6499
BstYI r'GATC_y
Cuts at: 1972 2127 3034 3829 4196 4581 4598 6050 6136
Size: 155 907 795 367 385 17 1452 86
Cuts at: 6136 6148 6494 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 907 795 385 367 346 155
86 17 12
BstZ17I GTA'TAC
Cuts at: 3052 3052
Size: 6499
BtgI C'CryG_G
Cuts at: 513 1065 2004 2881 3487 513
Size: 552 939 877 606 3525
Fragments arranged by size:
3525 939 877 606 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2080 487
Size: 683 56 854 4906
Fragments arranged by size:
4906 854 683 56
BtsI GCAGTG_nn'
Cuts at: 4015 4809 4829 4015
Size: 794 20 5685
Fragments arranged by size:
5685 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6499
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6464
DraI TTT'AAA
Cuts at: 3327 3803 3327
Size: 476 6023
DraIII CAC_nnn'GTG
Cuts at: 2427 2427
Size: 6499
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5506 809
Size: 507 4190 1802
Fragments arranged by size:
4190 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2132 2148
Size: 54 1425 50 275 18 249 53 16
Cuts at: 2148 3946 4835 6464 8
Size: 1798 889 1629 43
Fragments arranged by size:
1798 1629 1425 889 275 249 54 53
50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2132 3946 1537
Size: 275 320 1814 4090
Fragments arranged by size:
4090 1814 320 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2014 2078 2586 2663 3204 3301 4429 1353
Size: 661 64 508 77 541 97 1128 3423
Fragments arranged by size:
3423 1128 661 541 508 97 77 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5086 5460 5606 387
Size: 1064 138 3497 374 146 1280
Fragments arranged by size:
3497 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2437 2437
Size: 6499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2234 2343 2576 2621 3287 3476
Size: 393 584 18 109 233 45 666 189
Cuts at: 3476 3731 4562 5159 5946 6214 1239
Size: 255 831 597 787 268 1524
Fragments arranged by size:
1524 831 787 666 597 584 393 268
255 233 189 109 45 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2555 2555
Size: 6499
EcoO109I rG'GnC_Cy
Cuts at: 2296 2342 2452 2494 2605 2967 3112 3727 2296
Size: 46 110 42 111 362 145 615 5068
Fragments arranged by size:
5068 615 362 145 111 110 46 42
EcoRI G'AATT_C
Cuts at: 2753 3808 4186 2753
Size: 1055 378 5066
Fragments arranged by size:
5066 1055 378
EcoRV GAT'ATC
Cuts at: 2466 2466
Size: 6499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2315 3544 2315
Size: 1229 5270
FspI TGC'GCA
Cuts at: 2105 5005 2105
Size: 2900 3599
HaeII r_GCGC'y
Cuts at: 1678 2172 2203 3658 5646 1678
Size: 494 31 1455 1988 2531
Fragments arranged by size:
2531 1988 1455 494 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 2674 2706 3218
Size: 32 135 32 911 32 159 32 512
Cuts at: 3218 3250 5140 5172 5214 5246 1373
Size: 32 1890 32 42 32 2626
Fragments arranged by size:
2626 1890 911 512 159 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3001 3843 4089 669
Size: 988 281 1063 842 246 3079
Fragments arranged by size:
3079 1063 988 842 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6499
HpaI GTT'AAC
Cuts at: 4089 4089
Size: 6499
KpnI G_GTAC'C
Cuts at: 3352 3824 3352
Size: 472 6027
MfeI C'AATT_G
Cuts at: 4098 4098
Size: 6499
MluI A'CGCG_T
Cuts at: 6375 6375
Size: 6499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5613 5797 1305
Size: 4308 184 2007
Fragments arranged by size:
4308 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 2150 10
Size: 54 1425 343 249 69 4359
Fragments arranged by size:
4359 1425 343 249 69 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2483 2570 3540 4457 4816 4975
Size: 648 933 384 87 970 917 359 159
Cuts at: 4975 518
Size: 2042
Fragments arranged by size:
2042 970 933 917 648 384 359 159
87
MspA1I CmG'CkG
Cuts at: 1606 2109 2236 2762 2863 2892 3265 3454 3579
Size: 503 127 526 101 29 373 189 125
Cuts at: 3579 3759 3915 4592 5740 5985 1606
Size: 180 156 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 526 503 373 245 189
180 156 127 125 101 29
NaeI GCC'GGC
Cuts at: 2062 2090 2062
Size: 28 6471
NcoI C'CATG_G
Cuts at: 513 1065 2004 3487 513
Size: 552 939 1483 3525
Fragments arranged by size:
3525 1483 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6499
NgoMIV G'CCGG_C
Cuts at: 2060 2088 2060
Size: 28 6471
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6499
NotI GC'GGCC_GC
Cuts at: 3946 3946
Size: 6499
NspI r_CATG'y
Cuts at: 1132 1229 2911 3857 5402 1132
Size: 97 1682 946 1545 2229
Fragments arranged by size:
2229 1682 1545 946 97
PasI CC'CwG_GG
Cuts at: 1348 3547 1348
Size: 2199 4300
PciI A'CATG_T
Cuts at: 5398 5398
Size: 6499
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3619 1108
Size: 2511 3988
PfoI T'CCnGG_A
Cuts at: 2181 2793 3274 3618 2181
Size: 612 481 344 5062
Fragments arranged by size:
5062 612 481 344
PmeI GTTT'AAAC
Cuts at: 3803 3803
Size: 6499
PmlI CAC'GTG
Cuts at: 2749 2749
Size: 6499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4568 4600 6081 6113 4568
Size: 32 1481 32 4954
Fragments arranged by size:
4954 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2342 2452 2605 2967 3112 2342
Size: 110 153 362 145 5729
Fragments arranged by size:
5729 362 153 145 110
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6499
PsiI TTA'TAA
Cuts at: 4069 4069
Size: 6499
PstI C_TGCA'G
Cuts at: 830 3851 830
Size: 3021 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6499
PvuII CAG'CTG
Cuts at: 2236 2892 3759 2236
Size: 656 867 4976
Fragments arranged by size:
4976 867 656
RsrII CG'GwC_CG
Cuts at: 2514 2514
Size: 6499
SacI G_AGCT'C
Cuts at: 2439 2439
Size: 6499
SalI G'TCGA_C
Cuts at: 1936 3841 1936
Size: 1905 4594
SanDI GG'GwC_CC
Cuts at: 2342 2342
Size: 6499
SapI GCTCTTCn'nnn_
Cuts at: 2078 3204 3301 2078
Size: 1126 97 5276
Fragments arranged by size:
5276 1126 97
SbfI CC_TGCA'GG
Cuts at: 3851 3851
Size: 6499
ScaI AGT'ACT
Cuts at: 2444 4747 6459 2444
Size: 2303 1712 2484
Fragments arranged by size:
2484 2303 1712
SfcI C'TryA_G
Cuts at: 826 1045 3847 4982 5663 5854 826
Size: 219 2802 1135 681 191 1471
Fragments arranged by size:
2802 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6499
SmaI CCC'GGG
Cuts at: 1872 2641 3826 1872
Size: 769 1185 4545
Fragments arranged by size:
4545 1185 769
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2438 4603 5504 5766 820
Size: 197 926 495 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 495 262 197
SnaBI TAC'GTA
Cuts at: 493 4290 493
Size: 3797 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6499
SphI G_CATG'C
Cuts at: 1132 3857 1132
Size: 2725 3774
SspI AAT'ATT
Cuts at: 5 52 3067 3474 4423 5
Size: 47 3015 407 949 2081
Fragments arranged by size:
3015 2081 949 407 47
StuI AGG'CCT
Cuts at: 1517 2679 1517
Size: 1162 5337
StyI C'CwwG_G
Cuts at: 513 1065 2004 2257 2448 3487 3519 3938 513
Size: 552 939 253 191 1039 32 419 3074
Fragments arranged by size:
3074 1039 939 552 419 253 191 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3530 4526 4543 4696 4881 5326 325
Size: 1255 1950 996 17 153 185 445 1498
Fragments arranged by size:
1950 1498 1255 996 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2442 2910 3243
Size: 274 80 33 51 1443 465 468 333
Cuts at: 3243 3648 4745 6457 96
Size: 405 1097 1712 138
Fragments arranged by size:
1712 1443 1097 468 465 405 333 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2888 2920 6459 6491 2888
Size: 32 3539 32 2896
Fragments arranged by size:
3539 2896 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3632 1316
Size: 2316 4183
XbaI T'CTAG_A
Cuts at: 3835 3835
Size: 6499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2354 2931 2354
Size: 577 5922
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6499
XmaI C'CCGG_G
Cuts at: 1870 2639 3824 1870
Size: 769 1185 4545
Fragments arranged by size:
4545 1185 769
XmnI GAAnn'nnTTC
Cuts at: 2565 3011 4628 2565
Size: 446 1617 4436
Fragments arranged by size:
4436 1617 446
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3433 4307 276
Size: 53 83 186 936 1899 874 2468
Fragments arranged by size:
2468 1899 936 874 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
Bsu36I FseI FspAI KasI NarI NruI NsiI PacI
PspOMI PspXI PsrI SacII SexAI SfiI SfoI SrfI
SwaI