Restriction map for
pFN21AB3632
(Circular) (Six-base) MAPSORT of: pFN21AB3632.seq Check: 7907 from: 1 to: 5806
pFN21AB3632 5806 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3149 1937
Size: 1212 4594
Acc65I G'GTAC_C
Cuts at: 2305 3127 2305
Size: 822 4984
AclI AA'CG_TT
Cuts at: 1563 3105 3933 4306 1563
Size: 1542 828 373 3063
Fragments arranged by size:
3063 1542 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2066 3869 5253 5521 1632
Size: 434 1803 1384 268 1917
Fragments arranged by size:
1917 1803 1384 434 268
AfeI AGC'GCT
Cuts at: 2540 2540
Size: 5806
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 2177 2446 3049 4705 5682 1203
Size: 974 269 603 1656 977 1327
Fragments arranged by size:
1656 1327 977 974 603 269
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 4535 4535
Size: 5806
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5806
AlwNI CAG_nnn'CTG
Cuts at: 2221 2284 2977 3549 5121 2221
Size: 63 693 572 1572 2906
Fragments arranged by size:
2906 1572 693 572 63
ApaLI G'TGCA_C
Cuts at: 3863 5019 3863
Size: 1156 4650
ApoI r'AATT_y
Cuts at: 1493 3115 3341 3493 5602 1493
Size: 1622 226 152 2109 1697
Fragments arranged by size:
2109 1697 1622 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 2151 2877 3131 1870
Size: 73 208 726 254 4545
Fragments arranged by size:
4545 726 254 208 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2289 2322 2289
Size: 33 5773
BamHI G'GATC_C
Cuts at: 2371 3136 3503 2371
Size: 765 367 4674
Fragments arranged by size:
4674 765 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 2471 2633 2764 3056
Size: 325 217 627 518 166 162 131 292
Cuts at: 3056 3127 4582 618
Size: 71 1455 1842
Fragments arranged by size:
1842 1455 627 518 325 292 217 166
162 131 71
BanII G_rGCy'C
Cuts at: 1391 3125 1391
Size: 1734 4072
BbeI G_GCGC'C
Cuts at: 3060 3060
Size: 5806
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2900 928
Size: 971 1001 3834
Fragments arranged by size:
3834 1001 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3698 4908 3698
Size: 1210 4596
BclI T'GATC_A
Cuts at: 1548 2778 1548
Size: 1230 4576
BfrBI ATG'CAT
Cuts at: 2939 2939
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2121 4417 136
Size: 107 122 71 631 605 449 2296 1525
Fragments arranged by size:
2296 1525 631 605 449 122 107 71
BglII A'GATC_T
Cuts at: 5801 5801
Size: 5806
BlpI GC'TnA_GC
Cuts at: 3223 3223
Size: 5806
Bme1580I G_kGCm'C
Cuts at: 1790 2476 2638 2775 3867 5023 1790
Size: 686 162 137 1092 1156 2573
Fragments arranged by size:
2573 1156 1092 686 162 137
BmgBI CAC'GTC
Cuts at: 1318 2447 3089 1318
Size: 1129 642 4035
Fragments arranged by size:
4035 1129 642
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2294 3537 4495
Size: 416 53 83 399 24 877 1243 958
Cuts at: 4495 442
Size: 1753
Fragments arranged by size:
1753 1243 958 877 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2831 4466 1239
Size: 1592 1635 2579
Fragments arranged by size:
2579 1635 1592
Bpu10I CC'TnA_GC
Cuts at: 2529 2790 2529
Size: 261 5545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2500 3931 4796 5094 2500
Size: 1431 865 298 3212
Fragments arranged by size:
3212 1431 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2548 4469 882
Size: 617 1049 1921 2219
Fragments arranged by size:
2219 1921 1049 617
BsaAI yAC'GTr
Cuts at: 493 1204 2178 3597 493
Size: 711 974 1419 2702
Fragments arranged by size:
2702 1419 974 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3057 3613 3995 4630
Size: 53 83 186 936 1524 556 382 635
Cuts at: 4630 275
Size: 1451
Fragments arranged by size:
1524 1451 936 635 556 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2038 4239 4637 4911 5058 5469 2038
Size: 2201 398 274 147 411 2375
Fragments arranged by size:
2375 2201 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5581
Fragments arranged by size:
5581 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2828 2960 2963 1186
Size: 1642 132 3 4029
Fragments arranged by size:
4029 1642 132 3
BseYI C'CCAG_C
Cuts at: 2321 5009 5505 2321
Size: 2688 496 2622
Fragments arranged by size:
2688 2622 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5233
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3256 4017 5045 1540
Size: 275 186 1255 761 1028 2301
Fragments arranged by size:
2301 1255 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3867 3952 5023 3867
Size: 85 1071 4650
Fragments arranged by size:
4650 1071 85
BsmI GAATG_Cn'
Cuts at: 3317 3410 3317
Size: 93 5713
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2476 2638 2775 3125 3867 3952 5023
Size: 399 686 162 137 350 742 85 1071
Cuts at: 5023 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 686 399 350 162 137
85
BspEI T'CCGG_A
Cuts at: 2038 5469 2038
Size: 3431 2375
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3161 844
Size: 2317 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3693 1394
Size: 429 1870 3507
Fragments arranged by size:
3507 1870 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2642 2903 3003 4301 4475 65
Size: 1183 1394 261 100 1298 174 1396
Fragments arranged by size:
1396 1394 1298 1183 261 174 100
BsrFI r'CCGG_y
Cuts at: 1700 1810 4450 4637 1700
Size: 110 2640 187 2869
Fragments arranged by size:
2869 2640 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5806
BssSI C'ACGA_G
Cuts at: 3866 4878 3866
Size: 1012 4794
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2221 794
Size: 1427 4379
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 2296 1184
Size: 1112 4694
BstYI r'GATC_y
Cuts at: 1972 2371 3136 3503 3888 3905 5357 5443 5455
Size: 399 765 367 385 17 1452 86 12
Cuts at: 5455 5801 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 765 399 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2028 2190 2559 2028
Size: 162 369 5275
Fragments arranged by size:
5275 369 162
BtgI C'CryG_G
Cuts at: 513 1065 2004 2301 2477 2523 3019 513
Size: 552 939 297 176 46 496 3300
Fragments arranged by size:
3300 939 552 496 297 176 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2981 487
Size: 683 56 1755 3312
Fragments arranged by size:
3312 1755 683 56
BtsI GCAGTG_nn'
Cuts at: 2219 2702 3322 4116 4136 2219
Size: 483 620 794 20 3889
Fragments arranged by size:
3889 794 620 483 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2724 3253 4142
Size: 54 1425 50 275 18 894 529 889
Cuts at: 4142 5771 8
Size: 1629 43
Fragments arranged by size:
1629 1425 894 889 529 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3253 1537
Size: 275 1441 4090
Fragments arranged by size:
4090 1441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2340 2693 3736 1353
Size: 987 353 1043 3423
Fragments arranged by size:
3423 1043 987 353
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4393 4767 4913 387
Size: 1064 138 2804 374 146 1280
Fragments arranged by size:
2804 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2831 3869 4466 5253 5521 1239
Size: 393 434 765 1038 597 787 268 1524
Fragments arranged by size:
1524 1038 787 765 597 434 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2313 2313
Size: 5806
EcoO109I rG'GnC_Cy
Cuts at: 2024 2318 2353 2024
Size: 294 35 5477
Fragments arranged by size:
5477 294 35
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FspI TGC'GCA
Cuts at: 4312 4312
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 2542 3060 4953 1678
Size: 864 518 1893 2531
Fragments arranged by size:
2531 1893 864 518
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4447 4479 4521 4553 1373
Size: 32 135 32 2875 32 42 32 2626
Fragments arranged by size:
2875 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 3396 669
Size: 988 281 1212 246 3079
Fragments arranged by size:
3079 1212 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KasI G'GCGC_C
Cuts at: 3056 3056
Size: 5806
KpnI G_GTAC'C
Cuts at: 2309 3131 2309
Size: 822 4984
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2283 4920 5104 1305
Size: 978 2637 184 2007
Fragments arranged by size:
2637 2007 978 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2726 10
Size: 54 1425 343 894 3090
Fragments arranged by size:
3090 1425 894 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 3764 4123 4282 518
Size: 648 1128 1470 359 159 2042
Fragments arranged by size:
2042 1470 1128 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2455 2575 2650 3222 3899 5047 5292 1606
Size: 849 120 75 572 677 1148 245 2120
Fragments arranged by size:
2120 1148 849 677 572 245 120 75
NarI GG'CG_CC
Cuts at: 3057 3057
Size: 5806
NcoI C'CATG_G
Cuts at: 513 1065 2004 2301 2477 2523 3019 513
Size: 552 939 297 176 46 496 3300
Fragments arranged by size:
3300 939 552 496 297 176 46
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 3253 3253
Size: 5806
NsiI A_TGCA'T
Cuts at: 2941 2941
Size: 5806
NspI r_CATG'y
Cuts at: 1132 1229 2113 3053 3164 4709 1132
Size: 97 884 940 111 1545 2229
Fragments arranged by size:
2229 1545 940 884 111 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 3049 4705 3049
Size: 1656 4150
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2284 2541 1108
Size: 1176 257 4373
Fragments arranged by size:
4373 1176 257
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PmlI CAC'GTG
Cuts at: 2178 2178
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2024 2318 2353 2024
Size: 294 35 5477
Fragments arranged by size:
5477 294 35
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PstI C_TGCA'G
Cuts at: 830 2217 3158 830
Size: 1387 941 3478
Fragments arranged by size:
3478 1387 941
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
PvuII CAG'CTG
Cuts at: 2575 2650 2575
Size: 75 5731
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SapI GCTCTTCn'nnn_
Cuts at: 2340 2693 2340
Size: 353 5453
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 4054 5766 4054
Size: 1712 4094
SfcI C'TryA_G
Cuts at: 826 1045 2213 3154 4289 4970 5161 826
Size: 219 1168 941 1135 681 191 1471
Fragments arranged by size:
1471 1168 1135 941 681 219 191
SfoI GGC'GCC
Cuts at: 3058 3058
Size: 5806
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 2153 2879 3133 1872
Size: 281 726 254 4545
Fragments arranged by size:
4545 726 281 254
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2515 3910 4811 5073 820
Size: 197 926 572 1395 901 262 1553
Fragments arranged by size:
1553 1395 926 901 572 262 197
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 2113 3164 1132
Size: 981 1051 3774
Fragments arranged by size:
3774 1051 981
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 2195 2760 1517
Size: 678 565 4563
Fragments arranged by size:
4563 678 565
StyI C'CwwG_G
Cuts at: 513 1065 2004 2301 2477 2523 3019 3245 513
Size: 552 939 297 176 46 496 226 3074
Fragments arranged by size:
3074 939 552 496 297 226 176 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3833 3850 4003 4188 4633 325
Size: 1255 2253 17 153 185 445 1498
Fragments arranged by size:
2253 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4052 5764 96
Size: 274 80 33 51 1443 2075 1712 138
Fragments arranged by size:
2075 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2610 2642 5766 5798 2610
Size: 32 3124 32 2618
Fragments arranged by size:
3124 2618 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 2151 2877 3131 1870
Size: 281 726 254 4545
Fragments arranged by size:
4545 726 281 254
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbvCI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI PacI PfoI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI XcmI