Restriction map for
pFN21AB3618
(Circular) (Six-base) MAPSORT of: pFN21AB3618.seq Check: 9342 from: 1 to: 5590
pFN21AB3618 5590 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2933 1937
Size: 996 4594
Acc65I G'GTAC_C
Cuts at: 2911 2911
Size: 5590
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 2639 2693 3653 5037 5305 1632
Size: 482 525 54 960 1384 268 1917
Fragments arranged by size:
1917 1384 960 525 482 268 54
AfeI AGC'GCT
Cuts at: 2726 2726
Size: 5590
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 4489 5466 1203
Size: 3286 977 1327
Fragments arranged by size:
3286 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AlwNI CAG_nnn'CTG
Cuts at: 3333 4905 3333
Size: 1572 4018
ApaI G_GGCC'C
Cuts at: 2886 2886
Size: 5590
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2899 3125 3277 5386 1493
Size: 1406 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2915 1870
Size: 73 972 4545
Fragments arranged by size:
4545 972 73
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2911 4366 618
Size: 325 217 627 1124 1455 1842
Fragments arranged by size:
1842 1455 1124 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2444 2886 2909 1391
Size: 1053 442 23 4072
Fragments arranged by size:
4072 1053 442 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2121 2647 928
Size: 971 222 526 3871
Fragments arranged by size:
3871 971 526 222
BbvCI CC'TCA_GC
Cuts at: 2381 2381
Size: 5590
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2416 2450 3781 3815 1903
Size: 34 479 34 1331 34 3678
Fragments arranged by size:
3678 1331 479 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 2634 1548
Size: 1086 4504
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2771 4201 136
Size: 107 122 71 631 605 1099 1430 1525
Fragments arranged by size:
1525 1430 1099 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2545 5585 2545
Size: 3040 2550
BlpI GC'TnA_GC
Cuts at: 3007 3007
Size: 5590
Bme1580I G_kGCm'C
Cuts at: 1790 2886 3651 4807 1790
Size: 1096 765 1156 2573
Fragments arranged by size:
2573 1156 1096 765
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5590
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 2513 2698
Size: 416 53 83 399 24 762 334 185
Cuts at: 2698 3321 4279 442
Size: 623 958 1753
Fragments arranged by size:
1753 958 762 623 416 399 334 185
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2288 2804 4250 1239
Size: 863 186 516 1446 2579
Fragments arranged by size:
2579 1446 863 516 186
Bpu10I CC'TnA_GC
Cuts at: 2069 2381 2069
Size: 312 5278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2845 3715 4580 4878 2845
Size: 870 865 298 3557
Fragments arranged by size:
3557 870 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4253 882
Size: 617 2754 2219
Fragments arranged by size:
2754 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3381 493
Size: 711 2177 2702
Fragments arranged by size:
2702 2177 711
BsaBI GATnn'nnATC
Cuts at: 2765 5584 2765
Size: 2819 2771
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2736 3397 3779 4414
Size: 53 83 186 936 1203 661 382 635
Cuts at: 4414 275
Size: 1451
Fragments arranged by size:
1451 1203 936 661 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4023 4421 4695 4842 5253 4023
Size: 398 274 147 411 4360
Fragments arranged by size:
4360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2264 2294 1178
Size: 30 165 30 597 30 234 30 4474
Fragments arranged by size:
4474 597 234 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 1186
Size: 1099 4491
BseYI C'CCAG_C
Cuts at: 2300 2539 2836 4793 5289 2300
Size: 239 297 1957 496 2601
Fragments arranged by size:
2601 1957 496 297 239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2382 1163
Size: 573 646 4371
Fragments arranged by size:
4371 646 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2024 3040 3801 4829 1540
Size: 275 186 23 1016 761 1028 2301
Fragments arranged by size:
2301 1028 1016 761 275 186 23
BsiHKAI G_wGCw'C
Cuts at: 2070 3651 3736 4807 2070
Size: 1581 85 1071 2853
Fragments arranged by size:
2853 1581 1071 85
BsmI GAATG_Cn'
Cuts at: 2192 2292 3101 3194 2192
Size: 100 809 93 4588
Fragments arranged by size:
4588 809 100 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2444 2886 2909 3651 3736 4807
Size: 399 280 374 442 23 742 85 1071
Cuts at: 4807 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 442 399 374 280 85
23
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 3479 5209 3479
Size: 1730 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2945 844
Size: 2101 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2026 3477 1394
Size: 429 203 1451 3507
Fragments arranged by size:
3507 1451 429 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 4085 4259 65
Size: 1183 2837 174 1396
Fragments arranged by size:
2837 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4234 4421 1700
Size: 110 2424 187 2869
Fragments arranged by size:
2869 2424 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 3650 4662 3650
Size: 1012 4578
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2549 794
Size: 1755 3835
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5590
BstYI r'GATC_y
Cuts at: 1972 2545 2920 3287 3672 3689 5141 5227 5239
Size: 573 375 367 385 17 1452 86 12
Cuts at: 5239 5585 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 573 385 375 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4099
Fragments arranged by size:
4099 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4851
Fragments arranged by size:
4851 683 56
BtsI GCAGTG_nn'
Cuts at: 3106 3900 3920 3106
Size: 794 20 4776
Fragments arranged by size:
4776 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5555
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2569 3037 3926
Size: 54 1425 50 275 18 739 468 889
Cuts at: 3926 5555 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 739 468 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 1537
Size: 275 1225 4090
Fragments arranged by size:
4090 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3520 1353
Size: 2167 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2795 4177 4551 4697 387
Size: 1064 138 1206 1382 374 146 1280
Fragments arranged by size:
1382 1280 1206 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2114 2288 2639 2693 2804 3653
Size: 393 470 12 174 351 54 111 849
Cuts at: 3653 4250 5037 5305 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 849 787 597 470 393 351 268
174 111 54 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2786 2807 2786
Size: 21 5569
EcoO109I rG'GnC_Cy
Cuts at: 2073 2201 2515 2883 2073
Size: 128 314 368 4780
Fragments arranged by size:
4780 368 314 128
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 2728 4737 1678
Size: 1050 2009 2531
Fragments arranged by size:
2531 2009 1050
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2264 2296 4231
Size: 32 135 32 428 32 232 32 1935
Cuts at: 4231 4263 4305 4337 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1935 428 232 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3180 669
Size: 988 281 996 246 3079
Fragments arranged by size:
3079 996 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KpnI G_GTAC'C
Cuts at: 2915 2915
Size: 5590
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 4704 4888 1305
Size: 927 2472 184 2007
Fragments arranged by size:
2472 2007 927 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2571 10
Size: 54 1425 343 739 3029
Fragments arranged by size:
3029 1425 739 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3548 3907 4066 518
Size: 648 2382 359 159 2042
Fragments arranged by size:
2382 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2092 2461 3006 3683 4831 5076 1606
Size: 486 369 545 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 545 486 369 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4099
Fragments arranged by size:
4099 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2948 4493 1132
Size: 97 1719 1545 2229
Fragments arranged by size:
2229 1719 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5590
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2815 1108
Size: 1707 3883
PfoI T'CCnGG_A
Cuts at: 2781 2781
Size: 5590
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2073 2515 2073
Size: 442 5148
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PspOMI G'GGCC_C
Cuts at: 2882 2882
Size: 5590
PstI C_TGCA'G
Cuts at: 830 2346 2942 830
Size: 1516 596 3478
Fragments arranged by size:
3478 1516 596
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
PvuII CAG'CTG
Cuts at: 2461 2461
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 2687 3838 5550 2687
Size: 1151 1712 2727
Fragments arranged by size:
2727 1712 1151
SexAI A'CCwGG_T
Cuts at: 2452 2452
Size: 5590
SfcI C'TryA_G
Cuts at: 826 1045 2342 2938 4073 4754 4945 826
Size: 219 1297 596 1135 681 191 1471
Fragments arranged by size:
1471 1297 1135 681 596 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2824 3694 4595 4857 820
Size: 197 926 881 870 901 262 1553
Fragments arranged by size:
1553 926 901 881 870 262 197
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2948 1132
Size: 1816 3774
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 2004 3029 513
Size: 552 939 1025 3074
Fragments arranged by size:
3074 1025 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2359 3617 3634 3787 3972 4417 325
Size: 1255 779 1258 17 153 185 445 1498
Fragments arranged by size:
1498 1258 1255 779 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2685 3836 5548
Size: 274 80 33 51 1443 708 1151 1712
Cuts at: 5548 96
Size: 138
Fragments arranged by size:
1712 1443 1151 708 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5550 5582 5550
Size: 32 5558
Tth111I GACn'n_nGTC
Cuts at: 1316 2454 1316
Size: 1138 4452
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI