Restriction map for
pFN21AB3604
(Circular) (Six-base) MAPSORT of: pFN21AB3604.seq Check: 9027 from: 1 to: 6205
pFN21AB3604 6205 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2109 3548 1937
Size: 172 1439 4594
Fragments arranged by size:
4594 1439 172
Acc65I G'GTAC_C
Cuts at: 2538 3279 3526 2538
Size: 741 247 5217
Fragments arranged by size:
5217 741 247
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3052 3427 4268 5652 5920 1632
Size: 1420 375 841 1384 268 1917
Fragments arranged by size:
1917 1420 1384 841 375 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 5104 6081 1203
Size: 3901 977 1327
Fragments arranged by size:
3901 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2297 1184
Size: 1113 5092
AlwNI CAG_nnn'CTG
Cuts at: 2032 2213 3948 5520 2032
Size: 181 1735 1572 2717
Fragments arranged by size:
2717 1735 1572 181
ApaI G_GGCC'C
Cuts at: 3025 3025
Size: 6205
ApaLI G'TGCA_C
Cuts at: 4262 5418 4262
Size: 1156 5049
ApoI r'AATT_y
Cuts at: 1493 2117 3514 3740 3892 6001 1493
Size: 624 1397 226 152 2109 1697
Fragments arranged by size:
2109 1697 1397 624 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 3530 1870
Size: 73 1587 4545
Fragments arranged by size:
4545 1587 73
BamHI G'GATC_C
Cuts at: 2075 3535 3902 2075
Size: 1460 367 4378
Fragments arranged by size:
4378 1460 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 2538 3279 3314 3526
Size: 325 217 627 281 470 741 35 212
Cuts at: 3526 4981 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 741 627 470 325 281 217
212 35
BanII G_rGCy'C
Cuts at: 1391 2886 3025 3524 1391
Size: 1495 139 499 4072
Fragments arranged by size:
4072 1495 499 139
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2150 2864 3433 928
Size: 971 251 714 569 3700
Fragments arranged by size:
3700 971 714 569 251
BbvCI CC'TCA_GC
Cuts at: 3183 3183
Size: 6205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4396 4430 1903
Size: 34 2459 34 3678
Fragments arranged by size:
3678 2459 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4097 5307 4097
Size: 1210 4995
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3242 4816 136
Size: 107 122 71 631 605 1570 1574 1525
Fragments arranged by size:
1574 1570 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2165 2301 6200 2165
Size: 136 3899 2170
Fragments arranged by size:
3899 2170 136
BlpI GC'TnA_GC
Cuts at: 2677 3622 2677
Size: 945 5260
Bme1580I G_kGCm'C
Cuts at: 1790 3025 4266 5422 1790
Size: 1235 1241 1156 2573
Fragments arranged by size:
2573 1241 1235 1156
BmgBI CAC'GTC
Cuts at: 1318 2592 3058 1318
Size: 1274 466 4465
Fragments arranged by size:
4465 1274 466
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3043 3096 3280
Size: 416 53 83 399 24 1626 53 184
Cuts at: 3280 3936 4894 442
Size: 656 958 1753
Fragments arranged by size:
1753 1626 958 656 416 399 184 83
53 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2606 3254 4865 1239
Size: 1367 648 1611 2579
Fragments arranged by size:
2579 1611 1367 648
Bpu10I CC'TnA_GC
Cuts at: 3183 3183
Size: 6205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4330 5195 5493 4330
Size: 865 298 5042
Fragments arranged by size:
5042 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4868 882
Size: 617 3369 2219
Fragments arranged by size:
3369 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3996 493
Size: 711 2792 2702
Fragments arranged by size:
2792 2702 711
BsaBI GATnn'nnATC
Cuts at: 2714 6199 2714
Size: 3485 2720
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4012 4394 5029 275
Size: 53 83 186 936 2479 382 635 1451
Fragments arranged by size:
2479 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2410 2541 2993 3132 4638 5036 5310 5457 5868
Size: 131 452 139 1506 398 274 147 411
Cuts at: 5868 2410
Size: 2747
Fragments arranged by size:
2747 1506 452 411 398 274 147 139
131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5980
Fragments arranged by size:
5980 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 2594 2813 3012 3122 1186
Size: 1110 298 219 199 110 4269
Fragments arranged by size:
4269 1110 298 219 199 110
BseYI C'CCAG_C
Cuts at: 2025 2472 3049 3151 5408 5904 2025
Size: 447 577 102 2257 496 2326
Fragments arranged by size:
2326 2257 577 496 447 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2989 1163
Size: 573 1253 4379
Fragments arranged by size:
4379 1253 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3655 4416 5444 1540
Size: 275 186 1654 761 1028 2301
Fragments arranged by size:
2301 1654 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3033 4266 4351 5422 3033
Size: 1233 85 1071 3816
Fragments arranged by size:
3816 1233 1071 85
BsmI GAATG_Cn'
Cuts at: 2209 2286 3716 3809 2209
Size: 77 1430 93 4605
Fragments arranged by size:
4605 1430 93 77
BsmBI CGTCTCn'nnnn_
Cuts at: 2582 2582
Size: 6205
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2886 3025 3033 3524 4266 4351 5422
Size: 399 1096 139 8 491 742 85 1071
Cuts at: 5422 1391
Size: 2174
Fragments arranged by size:
2174 1096 1071 742 491 399 139 85
8
BspEI T'CCGG_A
Cuts at: 2410 5868 2410
Size: 3458 2747
BspHI T'CATG_A
Cuts at: 4094 5824 4094
Size: 1730 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3222 3560 844
Size: 2378 338 3489
Fragments arranged by size:
3489 2378 338
BsrBI CCG'CTC
Cuts at: 1394 1823 4092 1394
Size: 429 2269 3507
Fragments arranged by size:
3507 2269 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4700 4874 65
Size: 1183 3452 174 1396
Fragments arranged by size:
3452 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4849 5036 1700
Size: 110 3039 187 2869
Fragments arranged by size:
3039 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6205
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 4265 5277 4265
Size: 1012 5193
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2443 3233 3321 794
Size: 1649 790 88 3678
Fragments arranged by size:
3678 1649 790 88
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstXI CCAn_nnnn'nTGG
Cuts at: 2612 2612
Size: 6205
BstYI r'GATC_y
Cuts at: 1972 2075 2165 2287 2301 2827 3135 3535 3902
Size: 103 90 122 14 526 308 400 367
Cuts at: 3902 4287 4304 5756 5842 5854 6200 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 526 400 385 367 346 308
122 103 90 86 17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2484 2656 2667 2750 513
Size: 552 939 480 172 11 83 3968
Fragments arranged by size:
3968 939 552 480 172 83 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2722 2932 487
Size: 683 56 1496 210 3760
Fragments arranged by size:
3760 1496 683 210 56
BtsI GCAGTG_nn'
Cuts at: 3721 4515 4535 3721
Size: 794 20 5391
Fragments arranged by size:
5391 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2088 5212 809
Size: 507 772 3124 1802
Fragments arranged by size:
3124 1802 772 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2865 3652 4541
Size: 54 1425 50 275 18 1035 787 889
Cuts at: 4541 6170 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1035 889 787 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3652 1537
Size: 275 1840 4090
Fragments arranged by size:
4090 1840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2232 2766 2811 4135 1353
Size: 879 534 45 1324 3423
Fragments arranged by size:
3423 1324 879 534 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4792 5166 5312 387
Size: 1064 138 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2606 3052 3254 3427 4268 4865 5652
Size: 393 974 446 202 173 841 597 787
Cuts at: 5652 5920 1239
Size: 268 1524
Fragments arranged by size:
1524 974 841 787 597 446 393 268
202 173
EcoO109I rG'GnC_Cy
Cuts at: 2159 2608 3037 3157 3211 3244 2159
Size: 449 429 120 54 33 5120
Fragments arranged by size:
5120 449 429 120 54 33
EcoRI G'AATT_C
Cuts at: 3514 3892 3514
Size: 378 5827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 6205
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 2032 5352 1678
Size: 354 3320 2531
Fragments arranged by size:
3320 2531 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2379 2411 4846 4878 4920
Size: 32 135 32 807 32 2435 32 42
Cuts at: 4920 4952 1373
Size: 32 2626
Fragments arranged by size:
2626 2435 807 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2110 2383 3549 3795 669
Size: 988 281 172 273 1166 246 3079
Fragments arranged by size:
3079 1166 988 281 273 246 172
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6205
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KpnI G_GTAC'C
Cuts at: 2542 3283 3530 2542
Size: 741 247 5217
Fragments arranged by size:
5217 741 247
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2377 5319 5503 1305
Size: 1072 2942 184 2007
Fragments arranged by size:
2942 2007 1072 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4383
Fragments arranged by size:
4383 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2483 2531 4163 4522 4681 518
Size: 648 1317 48 1632 359 159 2042
Fragments arranged by size:
2042 1632 1317 648 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 2188 2808 3621 4298 5446 5691 1606
Size: 582 620 813 677 1148 245 2120
Fragments arranged by size:
2120 1148 813 677 620 582 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2484 2656 2667 513
Size: 552 939 480 172 11 4051
Fragments arranged by size:
4051 939 552 480 172 11
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6205
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 2377 3563 5108 1132
Size: 97 1148 1186 1545 2229
Fragments arranged by size:
2229 1545 1186 1148 97
PasI CC'CwG_GG
Cuts at: 1348 2156 1348
Size: 808 5397
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2032 1108
Size: 924 5281
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3157 3211 3157
Size: 54 6151
PshAI GACnn'nnGTC
Cuts at: 1613 2150 1613
Size: 537 5668
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PspOMI G'GGCC_C
Cuts at: 3021 3021
Size: 6205
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 6173
PstI C_TGCA'G
Cuts at: 830 2042 2101 3557 830
Size: 1212 59 1456 3478
Fragments arranged by size:
3478 1456 1212 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
PvuII CAG'CTG
Cuts at: 2188 2188
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 2108 3547 1936
Size: 172 1439 4594
Fragments arranged by size:
4594 1439 172
SanDI GG'GwC_CC
Cuts at: 3157 3157
Size: 6205
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SexAI A'CCwGG_T
Cuts at: 2256 2256
Size: 6205
SfcI C'TryA_G
Cuts at: 826 1045 2038 2097 2626 3553 4688 5369 5560
Size: 219 993 59 529 927 1135 681 191
Cuts at: 5560 826
Size: 1471
Fragments arranged by size:
1471 1135 993 927 681 529 219 191
59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 3532 1872
Size: 1660 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4309 5210 5472 820
Size: 197 926 2366 901 262 1553
Fragments arranged by size:
2366 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3563 1132
Size: 2431 3774
SspI AAT'ATT
Cuts at: 5 52 3475 4129 5
Size: 47 3423 654 2081
Fragments arranged by size:
3423 2081 654 47
StuI AGG'CCT
Cuts at: 1517 3118 1517
Size: 1601 4604
StyI C'CwwG_G
Cuts at: 513 1065 2004 2484 2656 2667 3644 513
Size: 552 939 480 172 11 977 3074
Fragments arranged by size:
3074 977 939 552 480 172 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3386 4232 4249 4402 4587 5032 325
Size: 1255 1806 846 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 846 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4451 6163 96
Size: 274 80 33 51 1443 2474 1712 138
Fragments arranged by size:
2474 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6165 6197 6165
Size: 32 6173
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6205
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2897 2897
Size: 6205
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 3530 1870
Size: 1660 4545
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI RsrII SacI SacII SapI
SfiI SfoI SrfI SwaI