Restriction map for
pFN21AB3598
(Circular) (Six-base) MAPSORT of: pFN21AB3598.seq Check: 7636 from: 1 to: 9202
pFN21AB3598 9202 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 7012 278
Size: 53 83 186 936 2155 3321 2468
Fragments arranged by size:
3321 2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6545 1937
Size: 4608 4594
Acc65I G'GTAC_C
Cuts at: 3317 5171 6523 3317
Size: 1854 1352 5996
Fragments arranged by size:
5996 1854 1352
AclI AA'CG_TT
Cuts at: 1563 7329 7702 1563
Size: 5766 373 3063
Fragments arranged by size:
5766 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2918 3216 3639 3774 4386 5438 5538 5781
Size: 1286 298 423 135 612 1052 100 243
Cuts at: 5781 5840 7265 8649 8917 1632
Size: 59 1425 1384 268 1917
Fragments arranged by size:
1917 1425 1384 1286 1052 612 423 298
268 243 135 100 59
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9005
AflIII A'CryG_T
Cuts at: 1203 5888 6008 8101 9078 1203
Size: 4685 120 2093 977 1327
Fragments arranged by size:
4685 2093 1327 977 120
AgeI A'CCGG_T
Cuts at: 8033 8033
Size: 9202
AhdI GACnn_n'nnGTC
Cuts at: 7931 7931
Size: 9202
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2715 1184
Size: 1531 7671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3112 3144 3526 3558 4696 4728 3112
Size: 32 382 32 1138 32 7586
Fragments arranged by size:
7586 1138 382 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3599 4769 4787 5633 6945 8517 3599
Size: 1170 18 846 1312 1572 4284
Fragments arranged by size:
4284 1572 1312 1170 846 18
ApaI G_GGCC'C
Cuts at: 2780 3231 3977 4418 5112 5642 2780
Size: 451 746 441 694 530 6340
Fragments arranged by size:
6340 746 694 530 451 441
ApaLI G'TGCA_C
Cuts at: 3075 7259 8415 3075
Size: 4184 1156 3862
Fragments arranged by size:
4184 3862 1156
ApoI r'AATT_y
Cuts at: 1493 2320 3390 6467 6511 6737 6889 8998 1493
Size: 827 1070 3077 44 226 152 2109 1697
Fragments arranged by size:
3077 2109 1697 1070 827 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 7756 160
Size: 872 6724 1606
Fragments arranged by size:
6724 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9202
AvaI C'yCGr_G
Cuts at: 1870 1943 3717 3836 4464 4491 4509 4808 5319
Size: 73 1774 119 628 27 18 299 511
Cuts at: 5319 6527 1870
Size: 1208 4545
Fragments arranged by size:
4545 1774 1208 628 511 299 119 73
27 18
AvrII C'CTAG_G
Cuts at: 4798 4798
Size: 9202
BamHI G'GATC_C
Cuts at: 3679 4649 6532 6899 3679
Size: 970 1883 367 5982
Fragments arranged by size:
5982 1883 970 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2993 3290 3317 5171 5252
Size: 325 217 627 1206 297 27 1854 81
Cuts at: 5252 6523 7978 618
Size: 1271 1455 1842
Fragments arranged by size:
1854 1842 1455 1271 1206 627 325 297
217 81 27
BanII G_rGCy'C
Cuts at: 1391 2780 2863 2881 3231 3311 3934 3977 4418
Size: 1389 83 18 350 80 623 43 441
Cuts at: 4418 4780 5112 5642 6521 1391
Size: 362 332 530 879 4072
Fragments arranged by size:
4072 1389 879 623 530 441 362 350
332 83 80 43 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 2461 2879 3248 4274 4391 4823
Size: 971 198 364 418 369 1026 117 432
Cuts at: 4823 5444 5453 928
Size: 621 9 4677
Fragments arranged by size:
4677 1026 971 621 432 418 369 364
198 117 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3004 3038 7393 7427 1903
Size: 34 1067 34 4355 34 3678
Fragments arranged by size:
4355 3678 1067 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3114 6227 7094 8304 3114
Size: 3113 867 1210 4012
Fragments arranged by size:
4012 3113 1210 867
BclI T'GATC_A
Cuts at: 1548 4084 5398 1548
Size: 2536 1314 5352
Fragments arranged by size:
5352 2536 1314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3306 3855
Size: 107 122 71 631 605 337 1297 549
Cuts at: 3855 4233 4485 5034 5439 5584 5664 5862 7813
Size: 378 252 549 405 145 80 198 1951
Cuts at: 7813 136
Size: 1525
Fragments arranged by size:
1951 1525 1297 631 605 549 549 405
378 337 252 198 145 122 107 80
71
BglII A'GATC_T
Cuts at: 2184 6430 9197 2184
Size: 4246 2767 2189
Fragments arranged by size:
4246 2767 2189
BlpI GC'TnA_GC
Cuts at: 3337 6619 3337
Size: 3282 5920
Bme1580I G_kGCm'C
Cuts at: 1790 2780 3079 3231 3977 4418 5112 5257 5642
Size: 990 299 152 746 441 694 145 385
Cuts at: 5642 5721 7263 8419 1790
Size: 79 1542 1156 2573
Fragments arranged by size:
2573 1542 1156 990 746 694 441 385
299 152 145 79
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2026 3234 5537
Size: 416 53 83 399 24 609 1208 2303
Cuts at: 5537 6933 7891 442
Size: 1396 958 1753
Fragments arranged by size:
2303 1753 1396 1208 958 609 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9202
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2228 3948 2228
Size: 1720 7482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2325 2397 2505 3234 3342 3531 3792 3912
Size: 1086 72 108 729 108 189 261 120
Cuts at: 3912 3930 3999 4035 4083 4119 4227 4281 4404
Size: 18 69 36 48 36 108 54 123
Cuts at: 4404 4461 4551 4596 4614 4746 4764 4794 4809
Size: 57 90 45 18 132 18 30 15
Cuts at: 4809 5268 5520 5583 7862 1239
Size: 459 252 63 2279 2579
Fragments arranged by size:
2579 2279 1086 729 459 261 252 189
132 123 120 108 108 108 90 72
69 63 57 54 48 45 36 36
30 18 18 18 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2584 3612 7327 8192 8490 2584
Size: 1028 3715 865 298 3296
Fragments arranged by size:
3715 3296 1028 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5211 5400 6117 6123 7865 882
Size: 617 3712 189 717 6 1742 2219
Fragments arranged by size:
3712 2219 1742 717 617 189 6
BsaAI yAC'GTr
Cuts at: 493 1204 2387 2765 5889 6041 6993 493
Size: 711 1183 378 3124 152 952 2702
Fragments arranged by size:
3124 2702 1183 952 711 378 152
BsaBI GATnn'nnATC
Cuts at: 9196 9196
Size: 9202
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3688 7009 7391 8026
Size: 53 83 186 936 2155 3321 382 635
Cuts at: 8026 275
Size: 1451
Fragments arranged by size:
3321 2155 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6327 7635 8033 8307 8454 8865 6327
Size: 1308 398 274 147 411 6664
Fragments arranged by size:
6664 1308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2701 2731 3295 3325 1178
Size: 30 165 30 1298 30 564 30 7055
Fragments arranged by size:
7055 1298 564 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2448 4014 4149 4176 5319 1186
Size: 841 421 1566 135 27 1143 5069
Fragments arranged by size:
5069 1566 1143 841 421 135 27
BseYI C'CCAG_C
Cuts at: 2591 5038 5348 5878 8405 8901 2591
Size: 2447 310 530 2527 496 2892
Fragments arranged by size:
2892 2527 2447 530 496 310
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2110 2721 1163
Size: 573 374 611 7644
Fragments arranged by size:
7644 611 573 374
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4971 6652 7413 8441 1540
Size: 275 186 2970 1681 761 1028 2301
Fragments arranged by size:
2970 2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2521 2863 2953 3061 3079 3574 3628 3718 3934
Size: 342 90 108 18 495 54 90 216
Cuts at: 3934 4780 5284 7263 7348 8419 2521
Size: 846 504 1979 85 1071 3304
Fragments arranged by size:
3304 1979 1071 846 504 495 342 216
108 90 90 85 54 18
BsmI GAATG_Cn'
Cuts at: 2234 2282 3027 6713 6806 2234
Size: 48 745 3686 93 4630
Fragments arranged by size:
4630 3686 745 93 48
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2521 2780 2863 2881 2953 3061 3079
Size: 399 731 259 83 18 72 108 18
Cuts at: 3079 3231 3311 3574 3628 3718 3934 3977 4418
Size: 152 80 263 54 90 216 43 441
Cuts at: 4418 4780 5112 5257 5284 5642 5721 6521 7263
Size: 362 332 145 27 358 79 800 742
Cuts at: 7263 7348 8419 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 800 742 731 441 399 362
358 332 263 259 216 152 145 108
90 85 83 80 79 72 54 43
27 18 18
BspEI T'CCGG_A
Cuts at: 8865 8865
Size: 9202
BspHI T'CATG_A
Cuts at: 7091 8821 7091
Size: 1730 7472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2179 3161 4421 4795 5222 6557 844
Size: 1335 982 1260 374 427 1335 3489
Fragments arranged by size:
3489 1335 1335 1260 982 427 374
BsrBI CCG'CTC
Cuts at: 1394 1823 7089 1394
Size: 429 5266 3507
Fragments arranged by size:
5266 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5170 7697 7871 65
Size: 1183 3922 2527 174 1396
Fragments arranged by size:
3922 2527 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2401 7846 8033 1700
Size: 110 591 5445 187 2869
Fragments arranged by size:
5445 2869 591 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9202
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9202
BssSI C'ACGA_G
Cuts at: 2466 2862 5382 7262 8274 2466
Size: 396 2520 1880 1012 3394
Fragments arranged by size:
3394 2520 1880 1012 396
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3905 794
Size: 3111 6091
BstBI TT'CG_AA
Cuts at: 8006 8006
Size: 9202
BstEII G'GTnAC_C
Cuts at: 1184 2903 3524 3875 1184
Size: 1719 621 351 6511
Fragments arranged by size:
6511 1719 621 351
BstYI r'GATC_y
Cuts at: 1972 2184 2849 3353 3679 4102 4649 5308 5740
Size: 212 665 504 326 423 547 659 432
Cuts at: 5740 5957 6430 6532 6899 7284 7301 8753 8839
Size: 217 473 102 367 385 17 1452 86
Cuts at: 8839 8851 9197 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 665 659 547 504 473 432
423 385 367 346 326 217 212 102
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2475 3204 3366 3384 5634 2475
Size: 729 162 18 2250 6043
Fragments arranged by size:
6043 2250 729 162 18
BtgI C'CryG_G
Cuts at: 513 1065 2997 513
Size: 552 1932 6718
Fragments arranged by size:
6718 1932 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8463
Fragments arranged by size:
8463 683 56
BtsI GCAGTG_nn'
Cuts at: 3877 5620 6718 7512 7532 3877
Size: 1743 1098 794 20 5547
Fragments arranged by size:
5547 1743 1098 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9167
DraI TTT'AAA
Cuts at: 6506 6506
Size: 9202
DraIII CAC_nnn'GTG
Cuts at: 3056 3497 3056
Size: 441 8761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2619 2748 2919 3108 4557 4827 5151
Size: 507 1303 129 171 189 1449 270 324
Cuts at: 5151 5340 8209 809
Size: 189 2869 1802
Fragments arranged by size:
2869 1802 1449 1303 507 324 270 189
189 171 129
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 3793 5683
Size: 54 1425 50 275 18 307 1656 1890
Cuts at: 5683 6649 7538 9167 8
Size: 966 889 1629 43
Fragments arranged by size:
1890 1656 1629 1425 966 889 307 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6649 1537
Size: 275 4837 4090
Fragments arranged by size:
4837 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 7132 1353
Size: 5779 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2036 4704 7789 8163 8309 387
Size: 1064 138 447 2668 3085 374 146 1280
Fragments arranged by size:
3085 2668 1280 1064 447 374 146 138
EcoICRI GAG'CTC
Cuts at: 2861 3932 4778 2861
Size: 1071 846 7285
Fragments arranged by size:
7285 1071 846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2325 2397 2505 2918 3216 3234 3342
Size: 393 693 72 108 413 298 18 108
Cuts at: 3342 3531 3639 3774 3792 3912 3930 3999 4035
Size: 189 108 135 18 120 18 69 36
Cuts at: 4035 4083 4119 4227 4281 4386 4404 4461 4551
Size: 48 36 108 54 105 18 57 90
Cuts at: 4551 4596 4614 4746 4764 4794 4809 5268 5438
Size: 45 18 132 18 30 15 459 170
Cuts at: 5438 5520 5538 5583 5781 5840 7265 7862 8649
Size: 82 18 45 198 59 1425 597 787
Cuts at: 8649 8917 1239
Size: 268 1524
Fragments arranged by size:
1524 1425 787 693 597 459 413 393
298 268 198 189 170 135 132 120
108 108 108 108 105 90 82 72
69 59 57 54 48 45 45 36
36 30 18 18 18 18 18 18
18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 4065 5952 4065
Size: 1887 7315
EcoO109I rG'GnC_Cy
Cuts at: 2408 2525 2552 2651 2705 2714 2776 2777 2930
Size: 117 27 99 54 9 62 1 153
Cuts at: 2930 3002 3110 3173 3191 3200 3228 3344 3362
Size: 72 108 63 18 9 28 116 18
Cuts at: 3362 3515 3641 3659 3749 3803 3857 4010 4019
Size: 153 126 18 90 54 54 153 9
Cuts at: 4019 4055 4127 4254 4406 4415 4433 4559 4766
Size: 36 72 127 152 9 18 126 207
Cuts at: 4766 4784 4793 4820 4840 4912 5108 5109 5324
Size: 18 9 27 20 72 196 1 215
Cuts at: 5324 5378 5531 5630 5638 5639 5675 2408
Size: 54 153 99 8 1 36 5935
Fragments arranged by size:
5935 215 207 196 153 153 153 153
152 127 126 126 117 116 108 99
99 90 72 72 72 63 62 54
54 54 54 36 36 28 27 27
20 18 18 18 18 18 9 9
9 9 9 8 1 1 1
EcoRI G'AATT_C
Cuts at: 3390 6467 6511 6889 3390
Size: 3077 44 378 5703
Fragments arranged by size:
5703 3077 378 44
EcoRV GAT'ATC
Cuts at: 5710 6301 5710
Size: 591 8611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3779 6403 3779
Size: 2624 6578
FspI TGC'GCA
Cuts at: 5852 7708 5852
Size: 1856 7346
HaeII r_GCGC'y
Cuts at: 1678 8349 1678
Size: 6671 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2591 2623 3718
Size: 32 135 32 450 32 537 32 1095
Cuts at: 3718 3750 4832 4864 6122 6154 6338 6370 7843
Size: 32 1082 32 1258 32 184 32 1473
Cuts at: 7843 7875 7917 7949 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1473 1258 1095 1082 537 450 184
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2510 6546 6792 669
Size: 988 281 572 4036 246 3079
Fragments arranged by size:
4036 3079 988 572 281 246
HindIII A'AGCT_T
Cuts at: 748 5903 748
Size: 5155 4047
HpaI GTT'AAC
Cuts at: 6792 6792
Size: 9202
KpnI G_GTAC'C
Cuts at: 3321 5175 6527 3321
Size: 1854 1352 5996
Fragments arranged by size:
5996 1854 1352
MfeI C'AATT_G
Cuts at: 5589 6801 5589
Size: 1212 7990
MluI A'CGCG_T
Cuts at: 9078 9078
Size: 9202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2577 2926 4735 5176 8316 8500 1305
Size: 1272 349 1809 441 3140 184 2007
Fragments arranged by size:
3140 2007 1809 1272 441 349 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2139 5685 10
Size: 54 1425 343 307 3546 3527
Fragments arranged by size:
3546 3527 1425 343 307 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4857 5724 7160 7519 7678 518
Size: 648 3691 867 1436 359 159 2042
Fragments arranged by size:
3691 2042 1436 867 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4376 4880 4961 5042 5159 5696 6618 7295
Size: 2770 504 81 81 117 537 922 677
Cuts at: 7295 8443 8688 1606
Size: 1148 245 2120
Fragments arranged by size:
2770 2120 1148 922 677 537 504 245
117 81 81
NcoI C'CATG_G
Cuts at: 513 1065 2997 513
Size: 552 1932 6718
Fragments arranged by size:
6718 1932 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9202
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9202
NotI GC'GGCC_GC
Cuts at: 6649 6649
Size: 9202
NspI r_CATG'y
Cuts at: 1132 1229 4996 5023 5737 6012 6560 8105 1132
Size: 97 3767 27 714 275 548 1545 2229
Fragments arranged by size:
3767 2229 1545 714 548 275 97 27
PasI CC'CwG_GG
Cuts at: 1348 2270 3881 4555 5222 5573 5801 1348
Size: 922 1611 674 667 351 228 4749
Fragments arranged by size:
4749 1611 922 674 667 351 228
PciI A'CATG_T
Cuts at: 6008 8101 6008
Size: 2093 7109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9202
PfoI T'CCnGG_A
Cuts at: 2395 2413 2521 2530 2737 2854 3061 3142 3151
Size: 18 108 9 207 117 207 81 9
Cuts at: 3151 3349 3358 3412 3682 3934 4060 4096 4105
Size: 198 9 54 270 252 126 36 9
Cuts at: 4105 4627 4681 4780 5284 2395
Size: 522 54 99 504 6313
Fragments arranged by size:
6313 522 504 270 252 207 207 198
126 117 108 99 81 54 54 36
18 9 9 9 9
PmeI GTTT'AAAC
Cuts at: 6506 6506
Size: 9202
PmlI CAC'GTG
Cuts at: 2765 5889 2765
Size: 3124 6078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7271 7303 8784 8816 7271
Size: 32 1481 32 7657
Fragments arranged by size:
7657 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2408 2525 2552 2651 2705 2714 3002 3110 3173
Size: 117 27 99 54 9 288 108 63
Cuts at: 3173 3200 3362 3515 3641 3659 3749 3803 4010
Size: 27 162 153 126 18 90 54 207
Cuts at: 4010 4127 4254 4406 4433 4559 4784 4793 4820
Size: 117 127 152 27 126 225 9 27
Cuts at: 4820 4840 4912 5324 5378 5531 5630 5675 2408
Size: 20 72 412 54 153 99 45 5935
Fragments arranged by size:
5935 412 288 225 207 162 153 153
152 127 126 126 117 117 108 99
99 90 72 63 54 54 54 45
27 27 27 27 20 18 9 9
PshAI GACnn'nnGTC
Cuts at: 1613 2539 5421 1613
Size: 926 2882 5394
Fragments arranged by size:
5394 2882 926
PsiI TTA'TAA
Cuts at: 6772 6772
Size: 9202
PspOMI G'GGCC_C
Cuts at: 2776 3227 3973 4414 5108 5638 2776
Size: 451 746 441 694 530 6340
Fragments arranged by size:
6340 746 694 530 451 441
PspXI vC'TCGA_Gb
Cuts at: 4464 4464
Size: 9202
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3265 3297 6194 6226 3265
Size: 32 2897 32 6241
Fragments arranged by size:
6241 2897 32 32
PstI C_TGCA'G
Cuts at: 830 5233 6554 830
Size: 4403 1321 3478
Fragments arranged by size:
4403 3478 1321
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9202
PvuII CAG'CTG
Cuts at: 4376 4880 4961 5159 5696 4376
Size: 504 81 198 537 7882
Fragments arranged by size:
7882 537 504 198 81
SacI G_AGCT'C
Cuts at: 2863 3934 4780 2863
Size: 1071 846 7285
Fragments arranged by size:
7285 1071 846
SalI G'TCGA_C
Cuts at: 1936 6544 1936
Size: 4608 4594
SanDI GG'GwC_CC
Cuts at: 4254 4793 4840 4912 5675 4254
Size: 539 47 72 763 7781
Fragments arranged by size:
7781 763 539 72 47
SbfI CC_TGCA'GG
Cuts at: 5233 6554 5233
Size: 1321 7881
ScaI AGT'ACT
Cuts at: 7450 9162 7450
Size: 1712 7490
SexAI A'CCwGG_T
Cuts at: 2494 2512 2728 2953 3124 4078 4276 4456 4933
Size: 18 216 225 171 954 198 180 477
Cuts at: 4933 5338 6047 2494
Size: 405 709 5649
Fragments arranged by size:
5649 954 709 477 405 225 216 198
180 171 18
SfcI C'TryA_G
Cuts at: 826 1045 2709 3177 3645 4725 5130 5229 5328
Size: 219 1664 468 468 1080 405 99 99
Cuts at: 5328 6550 7685 8366 8557 826
Size: 1222 1135 681 191 1471
Fragments arranged by size:
1664 1471 1222 1135 1080 681 468 468
405 219 191 99 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 3855 5034 5584 5664 3855
Size: 1179 550 80 7393
Fragments arranged by size:
7393 1179 550 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9202
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9202
SmaI CCC'GGG
Cuts at: 1872 3838 4493 4810 6529 1872
Size: 1966 655 317 1719 4545
Fragments arranged by size:
4545 1966 1719 655 317
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2563 3627 4464 4509 7306 8207
Size: 197 926 620 1064 837 45 2797 901
Cuts at: 8207 8469 820
Size: 262 1553
Fragments arranged by size:
2797 1553 1064 926 901 837 620 262
197 45
SnaBI TAC'GTA
Cuts at: 493 6993 493
Size: 6500 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9202
SphI G_CATG'C
Cuts at: 1132 4996 5023 5737 6560 1132
Size: 3864 27 714 823 3774
Fragments arranged by size:
3864 3774 823 714 27
SspI AAT'ATT
Cuts at: 5 52 6319 7126 5
Size: 47 6267 807 2081
Fragments arranged by size:
6267 2081 807 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9202
StyI C'CwwG_G
Cuts at: 513 1065 2663 2961 2997 4329 4708 4798 5374
Size: 552 1598 298 36 1332 379 90 576
Cuts at: 5374 5953 6641 513
Size: 579 688 3074
Fragments arranged by size:
3074 1598 1332 688 579 576 552 379
298 90 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2562 2981 3764 5267 5424 5892 7229
Size: 1255 982 419 783 1503 157 468 1337
Cuts at: 7229 7246 7399 7584 8029 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1503 1498 1337 1255 982 783 468 445
419 185 157 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 7448 9160
Size: 274 80 33 51 1443 168 5303 1712
Cuts at: 9160 96
Size: 138
Fragments arranged by size:
5303 1712 1443 274 168 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9162 9194 9162
Size: 32 9170
Tth111I GACn'n_nGTC
Cuts at: 1316 4215 1316
Size: 2899 6303
XbaI T'CTAG_A
Cuts at: 6538 6538
Size: 9202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2986 3004 4715 5381 6045 2986
Size: 18 1711 666 664 6143
Fragments arranged by size:
6143 1711 666 664 18
XhoI C'TCGA_G
Cuts at: 1943 4464 4509 1943
Size: 2521 45 6636
Fragments arranged by size:
6636 2521 45
XmaI C'CCGG_G
Cuts at: 1870 3836 4491 4808 6527 1870
Size: 1966 655 317 1719 4545
Fragments arranged by size:
4545 1966 1719 655 317
XmnI GAAnn'nnTTC
Cuts at: 7331 7331
Size: 9202
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 7010 276
Size: 53 83 186 936 2155 3321 2468
Fragments arranged by size:
3321 2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI RsrII SacII SapI
SfoI SrfI SwaI