Restriction map for
pFN21AB3596
(Circular) (Six-base) MAPSORT of: pFN21AB3596.seq Check: 3150 from: 1 to: 6214
pFN21AB3596 6214 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3557 1937
Size: 1620 4594
Acc65I G'GTAC_C
Cuts at: 2969 3535 2969
Size: 566 5648
AclI AA'CG_TT
Cuts at: 1563 2722 4341 4714 1563
Size: 1159 1619 373 3063
Fragments arranged by size:
3063 1619 1159 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3168 4277 5661 5929 1632
Size: 1536 1109 1384 268 1917
Fragments arranged by size:
1917 1536 1384 1109 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 5113 6090 1203
Size: 3910 977 1327
Fragments arranged by size:
3910 1327 977
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 4943 4943
Size: 6214
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6214
AlwNI CAG_nnn'CTG
Cuts at: 2570 2897 3957 5529 2570
Size: 327 1060 1572 3255
Fragments arranged by size:
3255 1572 1060 327
ApaLI G'TGCA_C
Cuts at: 2262 4271 5427 2262
Size: 2009 1156 3049
Fragments arranged by size:
3049 2009 1156
ApoI r'AATT_y
Cuts at: 1493 3261 3523 3749 3901 6010 1493
Size: 1768 262 226 152 2109 1697
Fragments arranged by size:
2109 1768 1697 262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2241 3539 1870
Size: 73 146 152 1298 4545
Fragments arranged by size:
4545 1298 152 146 73
BamHI G'GATC_C
Cuts at: 2861 3544 3911 2861
Size: 683 367 5164
Fragments arranged by size:
5164 683 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2969 3535 4990 618
Size: 325 217 627 1182 566 1455 1842
Fragments arranged by size:
1842 1455 1182 627 566 325 217
BanII G_rGCy'C
Cuts at: 1391 2163 3533 1391
Size: 772 1370 4072
Fragments arranged by size:
4072 1370 772
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4405 4439 1903
Size: 34 2468 34 3678
Fragments arranged by size:
3678 2468 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4825 136
Size: 107 122 71 631 605 337 2816 1525
Fragments arranged by size:
2816 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6209 6209
Size: 6214
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 2266 4275 5431 1790
Size: 476 2009 1156 2573
Fragments arranged by size:
2573 2009 1156 476
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2289 3945 4903
Size: 416 53 83 399 24 872 1656 958
Cuts at: 4903 442
Size: 1753
Fragments arranged by size:
1753 1656 958 872 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4874 1239
Size: 3635 2579
Bpu10I CC'TnA_GC
Cuts at: 2575 2575
Size: 6214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3415 4339 5204 5502 3415
Size: 924 865 298 4127
Fragments arranged by size:
4127 924 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2177 4877 882
Size: 617 678 2700 2219
Fragments arranged by size:
2700 2219 678 617
BsaAI yAC'GTr
Cuts at: 493 1204 4005 493
Size: 711 2801 2702
Fragments arranged by size:
2801 2702 711
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4021 4403 5038 275
Size: 53 83 186 936 2488 382 635 1451
Fragments arranged by size:
2488 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4647 5045 5319 5466 5877 4647
Size: 398 274 147 411 4984
Fragments arranged by size:
4984 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2673 2703 1178
Size: 30 165 30 1270 30 4689
Fragments arranged by size:
4689 1270 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2036 2698 1186
Size: 847 3 662 4702
Fragments arranged by size:
4702 847 662 3
BseYI C'CCAG_C
Cuts at: 5417 5913 5417
Size: 496 5718
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5641
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 3664 4425 5453 1540
Size: 275 186 18 1645 761 1028 2301
Fragments arranged by size:
2301 1645 1028 761 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2266 4275 4360 5431 2266
Size: 2009 85 1071 3049
Fragments arranged by size:
3049 2009 1071 85
BsmI GAATG_Cn'
Cuts at: 3725 3818 3725
Size: 93 6121
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2266 3533 4275 4360 5431 1391
Size: 399 373 103 1267 742 85 1071 2174
Fragments arranged by size:
2174 1267 1071 742 399 373 103 85
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 2770 2851 3265 4103 5833 2770
Size: 81 414 838 1730 3151
Fragments arranged by size:
3151 1730 838 414 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3569 844
Size: 2725 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2937 4101 1394
Size: 429 1114 1164 3507
Fragments arranged by size:
3507 1164 1114 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2317 2391 2585 2988 3219 3458 4709
Size: 1183 1069 74 194 403 231 239 1251
Cuts at: 4709 4883 65
Size: 174 1396
Fragments arranged by size:
1396 1251 1183 1069 403 239 231 194
174 74
BsrFI r'CCGG_y
Cuts at: 1700 1810 4858 5045 1700
Size: 110 3048 187 2869
Fragments arranged by size:
3048 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2126 96
Size: 2030 4184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6214
BssSI C'ACGA_G
Cuts at: 2048 4274 5286 2048
Size: 2226 1012 2976
Fragments arranged by size:
2976 2226 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6214
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6214
BstXI CCAn_nnnn'nTGG
Cuts at: 3375 3375
Size: 6214
BstYI r'GATC_y
Cuts at: 1972 2861 3544 3911 4296 4313 5765 5851 5863
Size: 889 683 367 385 17 1452 86 12
Cuts at: 5863 6209 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 889 683 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2928 513
Size: 552 939 924 3799
Fragments arranged by size:
3799 939 924 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5475
Fragments arranged by size:
5475 683 56
BtsI GCAGTG_nn'
Cuts at: 3730 4524 4544 3730
Size: 794 20 5400
Fragments arranged by size:
5400 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DraIII CAC_nnn'GTG
Cuts at: 3121 3121
Size: 6214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5221 809
Size: 507 3905 1802
Fragments arranged by size:
3905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3661 4550 6179
Size: 54 1425 50 275 18 1831 889 1629
Cuts at: 6179 8
Size: 43
Fragments arranged by size:
1831 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2275 2481 4144 1353
Size: 922 206 1663 3423
Fragments arranged by size:
3423 1663 922 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4801 5175 5321 387
Size: 1064 138 3212 374 146 1280
Fragments arranged by size:
3212 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3168 4277 4874 5661 5929 1239
Size: 393 1536 1109 597 787 268 1524
Fragments arranged by size:
1536 1524 1109 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2491 2491
Size: 6214
EcoRI G'AATT_C
Cuts at: 3261 3523 3901 3261
Size: 262 378 5574
Fragments arranged by size:
5574 378 262
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3037 3037
Size: 6214
FspI TGC'GCA
Cuts at: 4720 4720
Size: 6214
HaeII r_GCGC'y
Cuts at: 1678 2084 5361 1678
Size: 406 3277 2531
Fragments arranged by size:
3277 2531 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2846 2878 2993 3025 4855
Size: 32 135 32 1274 32 115 32 1830
Cuts at: 4855 4887 4929 4961 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1830 1274 135 115 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3494 3558 3804 669
Size: 988 281 1556 64 246 3079
Fragments arranged by size:
3079 1556 988 281 246 64
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6214
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KpnI G_GTAC'C
Cuts at: 2973 3539 2973
Size: 566 5648
MfeI C'AATT_G
Cuts at: 2604 3813 2604
Size: 1209 5005
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2363 5328 5512 1305
Size: 1058 2965 184 2007
Fragments arranged by size:
2965 2007 1058 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4392
Fragments arranged by size:
4392 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 2856 3170 3509 4172 4531 4690
Size: 648 1266 424 314 339 663 359 159
Cuts at: 4690 518
Size: 2042
Fragments arranged by size:
2042 1266 663 648 424 359 339 314
159
MspA1I CmG'CkG
Cuts at: 1606 2387 3630 4307 5455 5700 1606
Size: 781 1243 677 1148 245 2120
Fragments arranged by size:
2120 1243 1148 781 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4723
Fragments arranged by size:
4723 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 2215 3572 5117 1132
Size: 97 986 1357 1545 2229
Fragments arranged by size:
2229 1545 1357 986 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6214
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3121 1108
Size: 2013 4201
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 5796 5828 4283
Size: 32 1481 32 4669
Fragments arranged by size:
4669 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2491 2491
Size: 6214
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6214
PsiI TTA'TAA
Cuts at: 3784 3784
Size: 6214
PstI C_TGCA'G
Cuts at: 830 3566 830
Size: 2736 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
PvuII CAG'CTG
Cuts at: 2387 2387
Size: 6214
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SbfI CC_TGCA'GG
Cuts at: 3566 3566
Size: 6214
ScaI AGT'ACT
Cuts at: 4462 6174 4462
Size: 1712 4502
SfcI C'TryA_G
Cuts at: 826 1045 3562 4697 5378 5569 826
Size: 219 2517 1135 681 191 1471
Fragments arranged by size:
2517 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 2091 3541 1872
Size: 219 1450 4545
Fragments arranged by size:
4545 1450 219
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2241 3394 4318 5219 5481 820
Size: 197 926 298 1153 924 901 262 1553
Fragments arranged by size:
1553 1153 926 924 901 298 262 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3572 1132
Size: 2440 3774
SspI AAT'ATT
Cuts at: 5 52 4138 5
Size: 47 4086 2081
Fragments arranged by size:
4086 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6214
StyI C'CwwG_G
Cuts at: 513 1065 2004 2365 2974 3653 513
Size: 552 939 361 609 679 3074
Fragments arranged by size:
3074 939 679 609 552 361
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4241 4258 4411 4596 5041 325
Size: 1255 2661 17 153 185 445 1498
Fragments arranged by size:
2661 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2126 4460 6172
Size: 274 80 33 51 1443 149 2334 1712
Cuts at: 6172 96
Size: 138
Fragments arranged by size:
2334 1712 1443 274 149 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6174 6206 6174
Size: 32 6182
Tth111I GACn'n_nGTC
Cuts at: 1316 2560 1316
Size: 1244 4970
XbaI T'CTAG_A
Cuts at: 2711 3550 2711
Size: 839 5375
XhoI C'TCGA_G
Cuts at: 1943 2241 1943
Size: 298 5916
XmaI C'CCGG_G
Cuts at: 1870 2089 3539 1870
Size: 219 1450 4545
Fragments arranged by size:
4545 1450 219
XmnI GAAnn'nnTTC
Cuts at: 4343 4343
Size: 6214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI