Restriction map for
pFN21AB3587
(Circular) (Six-base) MAPSORT of: pFN21AB3587.seq Check: 6817 from: 1 to: 6169
pFN21AB3587 6169 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2519 3183 2519
Size: 664 5505
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3979 278
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3512 1937
Size: 1575 4594
Acc65I G'GTAC_C
Cuts at: 2439 3490 2439
Size: 1051 5118
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2329 4232 5616 5884 1632
Size: 697 1903 1384 268 1917
Fragments arranged by size:
1917 1903 1384 697 268
AflII C'TTAA_G
Cuts at: 820 1017 2738 820
Size: 197 1721 4251
Fragments arranged by size:
4251 1721 197
AflIII A'CryG_T
Cuts at: 1203 3069 5068 6045 1203
Size: 1866 1999 977 1327
Fragments arranged by size:
1999 1866 1327 977
AgeI A'CCGG_T
Cuts at: 5000 5000
Size: 6169
AhdI GACnn_n'nnGTC
Cuts at: 4898 4898
Size: 6169
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6169
AlwNI CAG_nnn'CTG
Cuts at: 2297 3319 3912 5484 2297
Size: 1022 593 1572 2982
Fragments arranged by size:
2982 1572 1022 593
ApaI G_GGCC'C
Cuts at: 2028 2958 3227 2028
Size: 930 269 4970
Fragments arranged by size:
4970 930 269
ApaLI G'TGCA_C
Cuts at: 4226 5382 4226
Size: 1156 5013
ApoI r'AATT_y
Cuts at: 1493 3104 3478 3704 3856 5965 1493
Size: 1611 374 226 152 2109 1697
Fragments arranged by size:
2109 1697 1611 374 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4723 160
Size: 872 3691 1606
Fragments arranged by size:
3691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 2134 3179 3494 1870
Size: 73 191 1045 315 4545
Fragments arranged by size:
4545 1045 315 191 73
BamHI G'GATC_C
Cuts at: 2867 3499 3866 2867
Size: 632 367 5170
Fragments arranged by size:
5170 632 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2423 2439 3334 3490 4945
Size: 325 217 627 636 16 895 156 1455
Cuts at: 4945 618
Size: 1842
Fragments arranged by size:
1842 1455 895 636 627 325 217 156
16
BanII G_rGCy'C
Cuts at: 1391 2028 2958 3150 3227 3488 1391
Size: 637 930 192 77 261 4072
Fragments arranged by size:
4072 930 637 261 192 77
BbeI G_GCGC'C
Cuts at: 3338 3338
Size: 6169
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2185 928
Size: 971 286 4912
Fragments arranged by size:
4912 971 286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3088 3122 4360 4394 1903
Size: 34 50 34 1067 34 1238 34 3678
Fragments arranged by size:
3678 1238 1067 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4061 5271 4061
Size: 1210 4959
BclI T'GATC_A
Cuts at: 1548 2578 2829 1548
Size: 1030 251 4888
Fragments arranged by size:
4888 1030 251
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3349 4780 136
Size: 107 122 71 631 605 1677 1431 1525
Fragments arranged by size:
1677 1525 1431 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6164 6164
Size: 6169
BlpI GC'TnA_GC
Cuts at: 3458 3586 3458
Size: 128 6041
Bme1580I G_kGCm'C
Cuts at: 1790 2028 2958 3200 3227 4230 5386 1790
Size: 238 930 242 27 1003 1156 2573
Fragments arranged by size:
2573 1156 1003 930 242 238 27
BmgBI CAC'GTC
Cuts at: 1318 2698 3280 1318
Size: 1380 582 4207
Fragments arranged by size:
4207 1380 582
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3230 3900 4858
Size: 416 53 83 399 24 1813 670 958
Cuts at: 4858 442
Size: 1753
Fragments arranged by size:
1813 1753 958 670 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3355 3355
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2783 4829 1239
Size: 1544 2046 2579
Fragments arranged by size:
2579 2046 1544
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4294 5159 5457 4294
Size: 865 298 5006
Fragments arranged by size:
5006 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4832 882
Size: 617 3333 2219
Fragments arranged by size:
3333 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3397 3960 493
Size: 711 2193 563 2702
Fragments arranged by size:
2702 2193 711 563
BsaBI GATnn'nnATC
Cuts at: 6163 6163
Size: 6169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2190 3323 3335 3976
Size: 53 83 186 936 657 1133 12 641
Cuts at: 3976 4358 4993 275
Size: 382 635 1451
Fragments arranged by size:
1451 1133 936 657 641 635 382 186
83 53 12
BsaWI w'CCGG_w
Cuts at: 4602 5000 5274 5421 5832 4602
Size: 398 274 147 411 4939
Fragments arranged by size:
4939 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2054 2084 1178
Size: 30 165 30 651 30 5263
Fragments arranged by size:
5263 651 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 1186
Size: 873 5296
BseYI C'CCAG_C
Cuts at: 3149 3400 5372 5868 3149
Size: 251 1972 496 3450
Fragments arranged by size:
3450 1972 496 251
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3155 1163
Size: 573 1419 4177
Fragments arranged by size:
4177 1419 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3619 4380 5408 1540
Size: 275 186 1618 761 1028 2301
Fragments arranged by size:
2301 1618 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3101 3303 4230 4315 5386 3101
Size: 202 927 85 1071 3884
Fragments arranged by size:
3884 1071 927 202 85
BsmI GAATG_Cn'
Cuts at: 2681 3090 3680 3773 2681
Size: 409 590 93 5077
Fragments arranged by size:
5077 590 409 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2958 3101 3150 3200 3227 3303
Size: 399 238 930 143 49 50 27 76
Cuts at: 3303 3488 4230 4315 5386 1391
Size: 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 930 742 399 238 185 143
85 76 50 49 27
BspEI T'CCGG_A
Cuts at: 5832 5832
Size: 6169
BspHI T'CATG_A
Cuts at: 2169 4058 5788 2169
Size: 1889 1730 2550
Fragments arranged by size:
2550 1889 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2519 3183 3524 844
Size: 1675 664 341 3489
Fragments arranged by size:
3489 1675 664 341
BsrBI CCG'CTC
Cuts at: 1394 1823 4056 1394
Size: 429 2233 3507
Fragments arranged by size:
3507 2233 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2542 4664 4838 65
Size: 1183 1294 2122 174 1396
Fragments arranged by size:
2122 1396 1294 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4813 5000 1700
Size: 110 3003 187 2869
Fragments arranged by size:
3003 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6169
BssSI C'ACGA_G
Cuts at: 2367 4229 5241 2367
Size: 1862 1012 3295
Fragments arranged by size:
3295 1862 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3204 794
Size: 2410 3759
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6169
BstXI CCAn_nnnn'nTGG
Cuts at: 3355 3355
Size: 6169
BstYI r'GATC_y
Cuts at: 1972 2867 3499 3866 4251 4268 5720 5806 5818
Size: 895 632 367 385 17 1452 86 12
Cuts at: 5818 6164 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 895 632 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2076 3465 513
Size: 552 1011 1389 3217
Fragments arranged by size:
3217 1389 1011 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5430
Fragments arranged by size:
5430 683 56
BtsI GCAGTG_nn'
Cuts at: 3685 4479 4499 3685
Size: 794 20 5355
Fragments arranged by size:
5355 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6134
DraI TTT'AAA
Cuts at: 3473 3473
Size: 6169
DraIII CAC_nnn'GTG
Cuts at: 3065 3065
Size: 6169
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2357 5176 809
Size: 507 1041 2819 1802
Fragments arranged by size:
2819 1802 1041 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2479 3408 3616
Size: 54 1425 50 275 18 649 929 208
Cuts at: 3616 4505 6134 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 929 889 649 275 208 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3616 1537
Size: 275 1804 4090
Fragments arranged by size:
4090 1804 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 2977 4099 1353
Size: 887 737 1122 3423
Fragments arranged by size:
3423 1122 887 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4756 5130 5276 387
Size: 1064 138 3167 374 146 1280
Fragments arranged by size:
3167 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2329 2783 4232 4829 5616 5884 1239
Size: 393 697 454 1449 597 787 268 1524
Fragments arranged by size:
1524 1449 787 697 597 454 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3260 3428 3260
Size: 168 6001
EcoO109I rG'GnC_Cy
Cuts at: 2325 2325
Size: 6169
EcoRI G'AATT_C
Cuts at: 3478 3856 3478
Size: 378 5791
EcoRV GAT'ATC
Cuts at: 2413 2413
Size: 6169
FspI TGC'GCA
Cuts at: 4675 4675
Size: 6169
HaeII r_GCGC'y
Cuts at: 1678 3338 5316 1678
Size: 1660 1978 2531
Fragments arranged by size:
2531 1978 1660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2976 3008 4810 4842 4884
Size: 32 135 32 1404 32 1802 32 42
Cuts at: 4884 4916 1373
Size: 32 2626
Fragments arranged by size:
2626 1802 1404 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3513 3759 669
Size: 988 281 1575 246 3079
Fragments arranged by size:
3079 1575 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KasI G'GCGC_C
Cuts at: 3334 3334
Size: 6169
KpnI G_GTAC'C
Cuts at: 2443 3494 2443
Size: 1051 5118
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2862 5283 5467 1305
Size: 1557 2421 184 2007
Fragments arranged by size:
2421 2007 1557 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2481 3410 10
Size: 54 1425 343 649 929 2769
Fragments arranged by size:
2769 1425 929 649 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3038 3395 4127 4486 4645 518
Size: 648 1872 357 732 359 159 2042
Fragments arranged by size:
2042 1872 732 648 359 357 159
MspA1I CmG'CkG
Cuts at: 1606 3585 4262 5410 5655 1606
Size: 1979 677 1148 245 2120
Fragments arranged by size:
2120 1979 1148 677 245
NarI GG'CG_CC
Cuts at: 3335 3335
Size: 6169
NcoI C'CATG_G
Cuts at: 513 1065 2076 513
Size: 552 1011 4606
Fragments arranged by size:
4606 1011 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 3073 3527 5072 1132
Size: 97 1844 454 1545 2229
Fragments arranged by size:
2229 1844 1545 454 97
PasI CC'CwG_GG
Cuts at: 1348 2592 1348
Size: 1244 4925
PciI A'CATG_T
Cuts at: 3069 5068 3069
Size: 1999 4170
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6169
PfoI T'CCnGG_A
Cuts at: 2760 2760
Size: 6169
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PmlI CAC'GTG
Cuts at: 3397 3397
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 5751 5783 4238
Size: 32 1481 32 4624
Fragments arranged by size:
4624 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2325 2325
Size: 6169
PshAI GACnn'nnGTC
Cuts at: 1613 2189 2207 1613
Size: 576 18 5575
Fragments arranged by size:
5575 576 18
PsiI TTA'TAA
Cuts at: 3739 3739
Size: 6169
PspOMI G'GGCC_C
Cuts at: 2024 2954 3223 2024
Size: 930 269 4970
Fragments arranged by size:
4970 930 269
PstI C_TGCA'G
Cuts at: 830 2293 3124 3141 3521 830
Size: 1463 831 17 380 3478
Fragments arranged by size:
3478 1463 831 380 17
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SanDI GG'GwC_CC
Cuts at: 2325 2325
Size: 6169
SbfI CC_TGCA'GG
Cuts at: 3521 3521
Size: 6169
ScaI AGT'ACT
Cuts at: 4417 6129 4417
Size: 1712 4457
SfcI C'TryA_G
Cuts at: 826 1045 2289 2623 2908 3120 3137 3517 4652
Size: 219 1244 334 285 212 17 380 1135
Cuts at: 4652 5333 5524 826
Size: 681 191 1471
Fragments arranged by size:
1471 1244 1135 681 380 334 285 219
212 191 17
SfoI GGC'GCC
Cuts at: 3336 3336
Size: 6169
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6169
SmaI CCC'GGG
Cuts at: 1872 2136 3181 3496 1872
Size: 264 1045 315 4545
Fragments arranged by size:
4545 1045 315 264
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2738 4273 5174 5436 820
Size: 197 926 795 1535 901 262 1553
Fragments arranged by size:
1553 1535 926 901 795 262 197
SnaBI TAC'GTA
Cuts at: 493 3960 493
Size: 3467 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 3527 1132
Size: 2395 3774
SspI AAT'ATT
Cuts at: 5 52 4093 5
Size: 47 4041 2081
Fragments arranged by size:
4041 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6169
StyI C'CwwG_G
Cuts at: 513 1065 2076 3608 513
Size: 552 1011 1532 3074
Fragments arranged by size:
3074 1532 1011 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2075 2708 4196 4213 4366 4551 4996
Size: 1255 495 633 1488 17 153 185 445
Cuts at: 4996 325
Size: 1498
Fragments arranged by size:
1498 1488 1255 633 495 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2043 4415 6127
Size: 274 80 33 51 1443 66 2372 1712
Cuts at: 6127 96
Size: 138
Fragments arranged by size:
2372 1712 1443 274 138 80 66 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2054 2086 6129 6161 2054
Size: 32 4043 32 2062
Fragments arranged by size:
4043 2062 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6169
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3217 3397 3217
Size: 180 5989
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 2134 3179 3494 1870
Size: 264 1045 315 4545
Fragments arranged by size:
4545 1045 315 264
XmnI GAAnn'nnTTC
Cuts at: 4298 4298
Size: 6169
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3977 276
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SrfI SwaI