Restriction map for
pFN21AB3574
(Circular) (Six-base) MAPSORT of: pFN21AB3574.seq Check: 946 from: 1 to: 6001
pFN21AB3574 6001 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2792 2792
Size: 6001
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2673 3344 1937
Size: 736 671 4594
Fragments arranged by size:
4594 736 671
Acc65I G'GTAC_C
Cuts at: 3322 3322
Size: 6001
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4064 5448 5716 1632
Size: 2432 1384 268 1917
Fragments arranged by size:
2432 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 4900 5877 1203
Size: 3697 977 1327
Fragments arranged by size:
3697 1327 977
AgeI A'CCGG_T
Cuts at: 4832 4832
Size: 6001
AhdI GACnn_n'nnGTC
Cuts at: 4730 4730
Size: 6001
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2060 3306 1184
Size: 876 1246 3879
Fragments arranged by size:
3879 1246 876
AlwNI CAG_nnn'CTG
Cuts at: 2540 2908 3744 5316 2540
Size: 368 836 1572 3225
Fragments arranged by size:
3225 1572 836 368
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 2056 2213 2260 3310 3536 3688 5797 1493
Size: 563 157 47 1050 226 152 2109 1697
Fragments arranged by size:
2109 1697 1050 563 226 157 152 47
AseI AT'TA_AT
Cuts at: 160 1032 2438 4555 160
Size: 872 1406 2117 1606
Fragments arranged by size:
2117 1606 1406 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 3326 1870
Size: 73 1383 4545
Fragments arranged by size:
4545 1383 73
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3322 4777 618
Size: 325 217 627 1535 1455 1842
Fragments arranged by size:
1842 1535 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2652 2769 3320 1391
Size: 1261 117 551 4072
Fragments arranged by size:
4072 1261 551 117
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2067 2576 928
Size: 971 168 509 4353
Fragments arranged by size:
4353 971 509 168
BbvCI CC'TCA_GC
Cuts at: 2496 2496
Size: 6001
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4192 4226 1903
Size: 34 2255 34 3678
Fragments arranged by size:
3678 2255 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2955 3893 5103 2955
Size: 938 1210 3853
Fragments arranged by size:
3853 1210 938
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BfrBI ATG'CAT
Cuts at: 2402 3125 2402
Size: 723 5278
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4612 136
Size: 107 122 71 631 605 2940 1525
Fragments arranged by size:
2940 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5996 5996
Size: 6001
BlpI GC'TnA_GC
Cuts at: 2637 3418 2637
Size: 781 5220
Bme1580I G_kGCm'C
Cuts at: 1790 4062 5218 1790
Size: 2272 1156 2573
Fragments arranged by size:
2573 2272 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2107 3732 4690
Size: 416 53 83 399 24 690 1625 958
Cuts at: 4690 442
Size: 1753
Fragments arranged by size:
1753 1625 958 690 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2562 4661 1239
Size: 1323 2099 2579
Fragments arranged by size:
2579 2099 1323
Bpu10I CC'TnA_GC
Cuts at: 2220 2496 2220
Size: 276 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4126 4991 5289 4126
Size: 865 298 4838
Fragments arranged by size:
4838 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4664 882
Size: 617 3165 2219
Fragments arranged by size:
3165 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3019 3792 493
Size: 711 1815 773 2702
Fragments arranged by size:
2702 1815 773 711
BsaBI GATnn'nnATC
Cuts at: 2624 5995 2624
Size: 3371 2630
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3808 4190 4825 275
Size: 53 83 186 936 2275 382 635 1451
Fragments arranged by size:
2275 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4434 4832 5106 5253 5664 4434
Size: 398 274 147 411 4771
Fragments arranged by size:
4771 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5776
Fragments arranged by size:
5776 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2082 1186
Size: 896 5105
BseYI C'CCAG_C
Cuts at: 2908 3027 5204 5700 2908
Size: 119 2177 496 3209
Fragments arranged by size:
3209 2177 496 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2883 1163
Size: 573 1147 4281
Fragments arranged by size:
4281 1147 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3451 4212 5240 1540
Size: 275 186 1450 761 1028 2301
Fragments arranged by size:
2301 1450 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2373 4062 4147 5218 2373
Size: 1689 85 1071 3156
Fragments arranged by size:
3156 1689 1071 85
BsmI GAATG_Cn'
Cuts at: 3512 3605 3512
Size: 93 5908
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2373 2652 2769 3320 4062 4147 5218
Size: 399 583 279 117 551 742 85 1071
Cuts at: 5218 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 583 551 399 279 117
85
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 3890 5620 3890
Size: 1730 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2537 2792 3356 844
Size: 1693 255 564 3489
Fragments arranged by size:
3489 1693 564 255
BsrBI CCG'CTC
Cuts at: 1394 1823 3888 1394
Size: 429 2065 3507
Fragments arranged by size:
3507 2065 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3273 4496 4670 65
Size: 1183 2025 1223 174 1396
Fragments arranged by size:
2025 1396 1223 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4645 4832 1700
Size: 110 2835 187 2869
Fragments arranged by size:
2869 2835 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6001
BssSI C'ACGA_G
Cuts at: 2372 4061 5073 2372
Size: 1689 1012 3300
Fragments arranged by size:
3300 1689 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6001
BstBI TT'CG_AA
Cuts at: 2264 2799 4805 2264
Size: 535 2006 3460
Fragments arranged by size:
3460 2006 535
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6001
BstXI CCAn_nnnn'nTGG
Cuts at: 2830 3031 2830
Size: 201 5800
BstYI r'GATC_y
Cuts at: 1972 2424 3331 3698 4083 4100 5552 5638 5650
Size: 452 907 367 385 17 1452 86 12
Cuts at: 5650 5996 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 907 452 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2250 513
Size: 552 939 246 4264
Fragments arranged by size:
4264 939 552 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5262
Fragments arranged by size:
5262 683 56
BtsI GCAGTG_nn'
Cuts at: 2025 2638 3517 4311 4331 2025
Size: 613 879 794 20 3695
Fragments arranged by size:
3695 879 794 613 20
ClaI AT'CG_AT
Cuts at: 1554 3288 1554
Size: 1734 4267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DraIII CAC_nnn'GTG
Cuts at: 3010 3010
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2071 5008 809
Size: 507 755 2937 1802
Fragments arranged by size:
2937 1802 755 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3021 3111 3448
Size: 54 1425 50 275 18 1191 90 337
Cuts at: 3448 4337 5966 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1191 889 337 275 90 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3448 1537
Size: 275 1636 4090
Fragments arranged by size:
4090 1636 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3931 1353
Size: 2578 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4588 4962 5108 387
Size: 1064 138 2999 374 146 1280
Fragments arranged by size:
2999 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2562 4064 4661 5448 5716 1239
Size: 393 930 1502 597 787 268 1524
Fragments arranged by size:
1524 1502 930 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2534 2534
Size: 6001
EcoO109I rG'GnC_Cy
Cuts at: 2011 2995 2011
Size: 984 5017
EcoRI G'AATT_C
Cuts at: 3310 3688 3310
Size: 378 5623
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 5148 1678
Size: 3470 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2077 2109 4642 4674 4716
Size: 32 135 32 505 32 2533 32 42
Cuts at: 4716 4748 1373
Size: 32 2626
Fragments arranged by size:
2626 2533 505 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2326 2793 3345 3591 669
Size: 988 281 388 467 552 246 3079
Fragments arranged by size:
3079 988 552 467 388 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6001
HpaI GTT'AAC
Cuts at: 2793 3591 2793
Size: 798 5203
KpnI G_GTAC'C
Cuts at: 3326 3326
Size: 6001
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3025 3279 5115 5299 1305
Size: 1720 254 1836 184 2007
Fragments arranged by size:
2007 1836 1720 254 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3023 3113 10
Size: 54 1425 343 1191 90 2898
Fragments arranged by size:
2898 1425 1191 343 90 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3288 3959 4318 4477 518
Size: 648 2122 671 359 159 2042
Fragments arranged by size:
2122 2042 671 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3031 3417 4094 5242 5487 1606
Size: 1425 386 677 1148 245 2120
Fragments arranged by size:
2120 1425 1148 677 386 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2250 513
Size: 552 939 246 4264
Fragments arranged by size:
4264 939 552 246
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NsiI A_TGCA'T
Cuts at: 2404 3127 2404
Size: 723 5278
NspI r_CATG'y
Cuts at: 1132 1229 2645 3359 4904 1132
Size: 97 1416 714 1545 2229
Fragments arranged by size:
2229 1545 1416 714 97
PacI TTA_AT'TAA
Cuts at: 2442 2442
Size: 6001
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6001
PciI A'CATG_T
Cuts at: 4900 4900
Size: 6001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2336 1108
Size: 1228 4773
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PmlI CAC'GTG
Cuts at: 3019 3019
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 5583 5615 4070
Size: 32 1481 32 4456
Fragments arranged by size:
4456 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PstI C_TGCA'G
Cuts at: 830 3353 830
Size: 2523 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 3031 3031
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 4249 5961 4249
Size: 1712 4289
SfcI C'TryA_G
Cuts at: 826 1045 2505 3349 4484 5165 5356 826
Size: 219 1460 844 1135 681 191 1471
Fragments arranged by size:
1471 1460 1135 844 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 3328 1872
Size: 1456 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4105 5006 5268 820
Size: 197 926 2162 901 262 1553
Fragments arranged by size:
2162 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 2645 3359 1132
Size: 1513 714 3774
Fragments arranged by size:
3774 1513 714
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6001
StyI C'CwwG_G
Cuts at: 513 1065 2004 2250 3440 513
Size: 552 939 246 1190 3074
Fragments arranged by size:
3074 1190 939 552 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3041 4028 4045 4198 4383 4828 325
Size: 1255 1461 987 17 153 185 445 1498
Fragments arranged by size:
1498 1461 1255 987 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4247 5959 96
Size: 274 80 33 51 1443 2270 1712 138
Fragments arranged by size:
2270 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2617 2649 5961 5993 2617
Size: 32 3312 32 2625
Fragments arranged by size:
3312 2625 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 3326 1870
Size: 1456 4545
XmnI GAAnn'nnTTC
Cuts at: 3212 4130 3212
Size: 918 5083
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI