Restriction map for
pFN21AB3573
(Circular) (Six-base) MAPSORT of: pFN21AB3573.seq Check: 822 from: 1 to: 6313
pFN21AB3573 6313 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4123 278
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3315 3333 3656 1937
Size: 1378 18 323 4594
Fragments arranged by size:
4594 1378 323 18
Acc65I G'GTAC_C
Cuts at: 2225 2482 3057 3634 2225
Size: 257 575 577 4904
Fragments arranged by size:
4904 577 575 257
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 2215 2472 2545 3373 4376 5760 6028
Size: 449 134 257 73 828 1003 1384 268
Cuts at: 6028 1632
Size: 1917
Fragments arranged by size:
1917 1384 1003 828 449 268 257 134
73
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 3348 5212 6189 1203
Size: 2145 1864 977 1327
Fragments arranged by size:
2145 1864 1327 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 5042 5042
Size: 6313
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2330 1184
Size: 1146 5167
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2328 2360 2328
Size: 32 6281
AlwNI CAG_nnn'CTG
Cuts at: 2384 2655 2866 4056 5628 2384
Size: 271 211 1190 1572 3069
Fragments arranged by size:
3069 1572 1190 271 211
ApaI G_GGCC'C
Cuts at: 2286 2286
Size: 6313
ApaLI G'TGCA_C
Cuts at: 4370 5526 4370
Size: 1156 5157
ApoI r'AATT_y
Cuts at: 1493 2425 3622 3848 4000 6109 1493
Size: 932 1197 226 152 2109 1697
Fragments arranged by size:
2109 1697 1197 932 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4867 160
Size: 872 3835 1606
Fragments arranged by size:
3835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 3638 1870
Size: 73 1695 4545
Fragments arranged by size:
4545 1695 73
AvrII C'CTAG_G
Cuts at: 2911 3091 2911
Size: 180 6133
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3041 3074 3041
Size: 33 6280
BamHI G'GATC_C
Cuts at: 3541 3643 4010 3541
Size: 102 367 5844
Fragments arranged by size:
5844 367 102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2225 2482 3057 3634
Size: 325 217 627 333 105 257 575 577
Cuts at: 3634 5089 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 577 575 333 325 257
217 105
BanII G_rGCy'C
Cuts at: 1391 2286 2614 3155 3413 3632 1391
Size: 895 328 541 258 219 4072
Fragments arranged by size:
4072 895 541 328 258 219
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3467 3501 4504 4538 1903
Size: 34 1530 34 1003 34 3678
Fragments arranged by size:
3678 1530 1003 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2843 4205 5415 2843
Size: 1362 1210 3741
Fragments arranged by size:
3741 1362 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6313
BfrBI ATG'CAT
Cuts at: 3472 3472
Size: 6313
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3140 4924 136
Size: 107 122 71 631 605 1468 1784 1525
Fragments arranged by size:
1784 1525 1468 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6308 6308
Size: 6313
BlpI GC'TnA_GC
Cuts at: 2784 3730 2784
Size: 946 5367
Bme1580I G_kGCm'C
Cuts at: 1790 2125 2286 2309 4374 5530 1790
Size: 335 161 23 2065 1156 2573
Fragments arranged by size:
2573 2065 1156 335 161 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3001 4044 5002
Size: 416 53 83 399 24 1584 1043 958
Cuts at: 5002 442
Size: 1753
Fragments arranged by size:
1753 1584 1043 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3586 3586
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3060 3581 4973 1239
Size: 1821 521 1392 2579
Fragments arranged by size:
2579 1821 1392 521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2573 2660 2696 4438 5303 5601 2573
Size: 87 36 1742 865 298 3285
Fragments arranged by size:
3285 1742 865 298 87 36
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4976 882
Size: 617 3477 2219
Fragments arranged by size:
3477 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4104 493
Size: 711 2900 2702
Fragments arranged by size:
2900 2702 711
BsaBI GATnn'nnATC
Cuts at: 6307 6307
Size: 6313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3604 4120 4502 5137
Size: 53 83 186 936 2071 516 382 635
Cuts at: 5137 275
Size: 1451
Fragments arranged by size:
2071 1451 936 635 516 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4746 5144 5418 5565 5976 4746
Size: 398 274 147 411 5083
Fragments arranged by size:
5083 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2177 2207 1178
Size: 30 165 30 774 30 5284
Fragments arranged by size:
5284 774 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3502 1186
Size: 2316 3997
BseYI C'CCAG_C
Cuts at: 2625 2834 5516 6012 2625
Size: 209 2682 496 2926
Fragments arranged by size:
2926 2682 496 209
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5740
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4524 5552 1540
Size: 275 186 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2790 4374 4459 5530 2790
Size: 1584 85 1071 3573
Fragments arranged by size:
3573 1584 1071 85
BsmI GAATG_Cn'
Cuts at: 3824 3917 3824
Size: 93 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2125 2286 2309 2614 2790 3155 3413
Size: 399 335 161 23 305 176 365 258
Cuts at: 3413 3632 4374 4459 5530 1391
Size: 219 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 365 335 305 258
219 176 161 85 23
BspEI T'CCGG_A
Cuts at: 5976 5976
Size: 6313
BspHI T'CATG_A
Cuts at: 4202 5932 4202
Size: 1730 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3668 844
Size: 2824 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2690 3510 4200 1394
Size: 429 867 820 690 3507
Fragments arranged by size:
3507 867 820 690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2182 2898 3042 4808 4982 65
Size: 1183 934 716 144 1766 174 1396
Fragments arranged by size:
1766 1396 1183 934 716 174 144
BsrFI r'CCGG_y
Cuts at: 1700 1810 4957 5144 1700
Size: 110 3147 187 2869
Fragments arranged by size:
3147 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3049 96
Size: 2953 3360
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 4373 5385 4373
Size: 1012 5301
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2120 3175 794
Size: 1326 1055 3932
Fragments arranged by size:
3932 1326 1055
BstBI TT'CG_AA
Cuts at: 5117 5117
Size: 6313
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstYI r'GATC_y
Cuts at: 1972 3541 3643 4010 4395 4412 5864 5950 5962
Size: 1569 102 367 385 17 1452 86 12
Cuts at: 5962 6308 1972
Size: 346 1977
Fragments arranged by size:
1977 1569 1452 385 367 346 102 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3405 3405
Size: 6313
BtgI C'CryG_G
Cuts at: 513 1065 2004 2487 513
Size: 552 939 483 4339
Fragments arranged by size:
4339 939 552 483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5574
Fragments arranged by size:
5574 683 56
BtsI GCAGTG_nn'
Cuts at: 2109 2260 2814 3465 3829 4623 4643 2109
Size: 151 554 651 364 794 20 3779
Fragments arranged by size:
3779 794 651 554 364 151 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6278
DraI TTT'AAA
Cuts at: 3617 3617
Size: 6313
DraIII CAC_nnn'GTG
Cuts at: 2255 2255
Size: 6313
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5320 809
Size: 507 4004 1802
Fragments arranged by size:
4004 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2490 3382 3760
Size: 54 1425 50 275 18 660 892 378
Cuts at: 3760 4649 6278 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 892 889 660 378 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 1537
Size: 275 1948 4090
Fragments arranged by size:
4090 1948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4243 1353
Size: 2890 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2939 4900 5274 5420 387
Size: 1064 138 1350 1961 374 146 1280
Fragments arranged by size:
1961 1350 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2215 2472 2545 3060 3373 3581
Size: 393 449 134 257 73 515 313 208
Cuts at: 3581 4376 4973 5760 6028 1239
Size: 795 597 787 268 1524
Fragments arranged by size:
1524 795 787 597 515 449 393 313
268 257 208 134 73
EcoNI CCTnn'n_nnAGG
Cuts at: 2570 2570
Size: 6313
EcoO109I rG'GnC_Cy
Cuts at: 2282 2915 3290 2282
Size: 633 375 5305
Fragments arranged by size:
5305 633 375
EcoRI G'AATT_C
Cuts at: 3622 4000 3622
Size: 378 5935
FspI TGC'GCA
Cuts at: 4819 4819
Size: 6313
HaeII r_GCGC'y
Cuts at: 1678 5460 1678
Size: 3782 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2457 2489 4954 4986 5028
Size: 32 135 32 885 32 2465 32 42
Cuts at: 5028 5060 1373
Size: 32 2626
Fragments arranged by size:
2626 2465 885 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3316 3657 3903 669
Size: 988 281 1378 341 246 3079
Fragments arranged by size:
3079 1378 988 341 281 246
HindIII A'AGCT_T
Cuts at: 748 2143 748
Size: 1395 4918
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KpnI G_GTAC'C
Cuts at: 2229 2486 3061 3638 2229
Size: 257 575 577 4904
Fragments arranged by size:
4904 577 575 257
MfeI C'AATT_G
Cuts at: 3295 3912 3295
Size: 617 5696
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5427 5611 1305
Size: 4122 184 2007
Fragments arranged by size:
4122 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 3384 10
Size: 54 1425 343 660 892 2939
Fragments arranged by size:
2939 1425 892 660 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2943 3477 4271 4630 4789 518
Size: 648 1777 534 794 359 159 2042
Fragments arranged by size:
2042 1777 794 648 534 359 159
MspA1I CmG'CkG
Cuts at: 1606 2496 2816 3729 4406 5554 5799 1606
Size: 890 320 913 677 1148 245 2120
Fragments arranged by size:
2120 1148 913 890 677 320 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2487 513
Size: 552 939 483 4339
Fragments arranged by size:
4339 939 552 483
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6313
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NsiI A_TGCA'T
Cuts at: 3474 3474
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 3671 5216 1132
Size: 97 2442 1545 2229
Fragments arranged by size:
2442 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6313
PciI A'CATG_T
Cuts at: 5212 5212
Size: 6313
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2655 2866 3162 3421 1108
Size: 1547 211 296 259 4000
Fragments arranged by size:
4000 1547 296 259 211
PfoI T'CCnGG_A
Cuts at: 2769 3558 2769
Size: 789 5524
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2915 3290 2915
Size: 375 5938
PshAI GACnn'nnGTC
Cuts at: 1613 2913 1613
Size: 1300 5013
PsiI TTA'TAA
Cuts at: 3883 3883
Size: 6313
PspOMI G'GGCC_C
Cuts at: 2282 2282
Size: 6313
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 6281
PstI C_TGCA'G
Cuts at: 830 2331 2619 3665 830
Size: 1501 288 1046 3478
Fragments arranged by size:
3478 1501 1046 288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
PvuII CAG'CTG
Cuts at: 2496 2816 2496
Size: 320 5993
SalI G'TCGA_C
Cuts at: 1936 3314 3655 1936
Size: 1378 341 4594
Fragments arranged by size:
4594 1378 341
SbfI CC_TGCA'GG
Cuts at: 3665 3665
Size: 6313
ScaI AGT'ACT
Cuts at: 2935 4561 6273 2935
Size: 1626 1712 2975
Fragments arranged by size:
2975 1712 1626
SfcI C'TryA_G
Cuts at: 826 1045 2152 2327 2615 3661 4796 5477 5668
Size: 219 1107 175 288 1046 1135 681 191
Cuts at: 5668 826
Size: 1471
Fragments arranged by size:
1471 1135 1107 1046 681 288 219 191
175
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 3640 1872
Size: 1768 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2588 2675 2711 4417 5318 5580
Size: 197 926 645 87 36 1706 901 262
Cuts at: 5580 820
Size: 1553
Fragments arranged by size:
1706 1553 926 901 645 262 197 87
36
SnaBI TAC'GTA
Cuts at: 493 4104 493
Size: 3611 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6313
SphI G_CATG'C
Cuts at: 1132 3671 1132
Size: 2539 3774
SspI AAT'ATT
Cuts at: 5 52 4237 5
Size: 47 4185 2081
Fragments arranged by size:
4185 2081 47
StuI AGG'CCT
Cuts at: 1517 2313 3143 1517
Size: 796 830 4687
Fragments arranged by size:
4687 830 796
StyI C'CwwG_G
Cuts at: 513 1065 2004 2221 2487 2911 3091 3342 3379
Size: 552 939 217 266 424 180 251 37
Cuts at: 3379 3752 513
Size: 373 3074
Fragments arranged by size:
3074 939 552 424 373 266 251 217
180 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2680 3272 3289 4340 4357 4510 4695
Size: 1255 1100 592 17 1051 17 153 185
Cuts at: 4695 5140 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1100 1051 592 445 185 153
17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2246 2933 3049
Size: 274 80 33 51 1443 269 687 116
Cuts at: 3049 3207 4559 6271 96
Size: 158 1352 1712 138
Fragments arranged by size:
1712 1443 1352 687 274 269 158 138
116 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6273 6305 6273
Size: 32 6281
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6313
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6313
XmaI C'CCGG_G
Cuts at: 1870 3638 1870
Size: 1768 4545
XmnI GAAnn'nnTTC
Cuts at: 4442 4442
Size: 6313
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4121 276
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BbeI BbvCI Bpu10I BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI