Restriction map for
pFN21AB3567
(Circular) (Six-base) MAPSORT of: pFN21AB3567.seq Check: 122 from: 1 to: 6847
pFN21AB3567 6847 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2836 2836
Size: 6847
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4657 278
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3339 4014 4190 1937
Size: 1402 675 176 4594
Fragments arranged by size:
4594 1402 675 176
Acc65I G'GTAC_C
Cuts at: 2194 4168 2194
Size: 1974 4873
AclI AA'CG_TT
Cuts at: 1563 4974 5347 1563
Size: 3411 373 3063
Fragments arranged by size:
3411 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3640 3742 4910 6294 6562 1632
Size: 2008 102 1168 1384 268 1917
Fragments arranged by size:
2008 1917 1384 1168 268 102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6650
AflIII A'CryG_T
Cuts at: 1203 5746 6723 1203
Size: 4543 977 1327
Fragments arranged by size:
4543 1327 977
AgeI A'CCGG_T
Cuts at: 5678 5678
Size: 6847
AhdI GACnn_n'nnGTC
Cuts at: 3244 5576 3244
Size: 2332 4515
AleI CACnn'nnGTG
Cuts at: 1166 2027 2042 1166
Size: 861 15 5971
Fragments arranged by size:
5971 861 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3278 1184
Size: 2094 4753
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 6815
AlwNI CAG_nnn'CTG
Cuts at: 2206 4590 6162 2206
Size: 2384 1572 2891
Fragments arranged by size:
2891 2384 1572
ApaLI G'TGCA_C
Cuts at: 2537 4904 6060 2537
Size: 2367 1156 3324
Fragments arranged by size:
3324 2367 1156
ApoI r'AATT_y
Cuts at: 1493 4156 4382 4534 6643 1493
Size: 2663 226 152 2109 1697
Fragments arranged by size:
2663 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5401 160
Size: 872 4369 1606
Fragments arranged by size:
4369 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
AvaI C'yCGr_G
Cuts at: 1870 1943 3414 3476 4172 1870
Size: 73 1471 62 696 4545
Fragments arranged by size:
4545 1471 696 73 62
BamHI G'GATC_C
Cuts at: 4177 4544 4177
Size: 367 6480
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2194 3122 3493 4168 5623
Size: 325 217 627 407 928 371 675 1455
Cuts at: 5623 618
Size: 1842
Fragments arranged by size:
1842 1455 928 675 627 407 371 325
217
BanII G_rGCy'C
Cuts at: 1391 2741 3513 3978 4002 4166 1391
Size: 1350 772 465 24 164 4072
Fragments arranged by size:
4072 1350 772 465 164 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5876
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5038 5072 1903
Size: 34 3101 34 3678
Fragments arranged by size:
3678 3101 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4739 5949 4739
Size: 1210 5637
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6847
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3306 5458 136
Size: 107 122 71 631 605 1634 2152 1525
Fragments arranged by size:
2152 1634 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3051 4087 6842 3051
Size: 1036 2755 3056
Fragments arranged by size:
3056 2755 1036
BlpI GC'TnA_GC
Cuts at: 4264 4264
Size: 6847
Bme1580I G_kGCm'C
Cuts at: 1790 2541 3498 4908 6064 1790
Size: 751 957 1410 1156 2573
Fragments arranged by size:
2573 1410 1156 957 751
BmgBI CAC'GTC
Cuts at: 1318 2488 1318
Size: 1170 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2049 2284 2348
Size: 416 53 83 399 24 632 235 64
Cuts at: 2348 2691 4578 5536 442
Size: 343 1887 958 1753
Fragments arranged by size:
1887 1753 958 632 416 399 343 235
83 64 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6847
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 6847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2584 2750 2752 2944 3068 5507 1239
Size: 1345 166 2 192 124 2439 2579
Fragments arranged by size:
2579 2439 1345 192 166 124 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2597 2705 3062 4972 5837 6135 2597
Size: 108 357 1910 865 298 3309
Fragments arranged by size:
3309 1910 865 357 298 108
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4046 5510 882
Size: 617 2547 1464 2219
Fragments arranged by size:
2547 2219 1464 617
BsaAI yAC'GTr
Cuts at: 493 1204 4638 493
Size: 711 3434 2702
Fragments arranged by size:
3434 2702 711
BsaBI GATnn'nnATC
Cuts at: 6841 6841
Size: 6847
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3072 4654 5036 5671
Size: 53 83 186 936 1539 1582 382 635
Cuts at: 5671 275
Size: 1451
Fragments arranged by size:
1582 1539 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2880 3352 3855 3876 5280 5678 5952 6099 6510
Size: 472 503 21 1404 398 274 147 411
Cuts at: 6510 2880
Size: 3217
Fragments arranged by size:
3217 1404 503 472 411 398 274 147
21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6622
Fragments arranged by size:
6622 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2926 3641 1186
Size: 841 899 715 4392
Fragments arranged by size:
4392 899 841 715
BseYI C'CCAG_C
Cuts at: 2080 2746 3166 3560 3817 6050 6546 2080
Size: 666 420 394 257 2233 496 2381
Fragments arranged by size:
2381 2233 666 496 420 394 257
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2020 2078 2381 1163
Size: 573 284 58 303 5629
Fragments arranged by size:
5629 573 303 284 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 3375 4014 4297 5058 6086
Size: 275 186 1309 65 639 283 761 1028
Cuts at: 6086 1540
Size: 2301
Fragments arranged by size:
2301 1309 1028 761 639 283 275 186
65
BsiHKAI G_wGCw'C
Cuts at: 2338 2541 3143 3267 3618 3978 4908 4993 6064
Size: 203 602 124 351 360 930 85 1071
Cuts at: 6064 2338
Size: 3121
Fragments arranged by size:
3121 1071 930 602 360 351 203 124
85
BsmI GAATG_Cn'
Cuts at: 2107 4358 4451 2107
Size: 2251 93 4503
Fragments arranged by size:
4503 2251 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3206 3417 3206
Size: 211 6636
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2338 2541 2741 3143 3267 3498 3513
Size: 399 548 203 200 402 124 231 15
Cuts at: 3513 3618 3978 4002 4166 4908 4993 6064 1391
Size: 105 360 24 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 548 402 399 360 231
203 200 164 124 105 85 24 15
BspEI T'CCGG_A
Cuts at: 2880 3352 3855 3876 6510 2880
Size: 472 503 21 2634 3217
Fragments arranged by size:
3217 2634 503 472 21
BspHI T'CATG_A
Cuts at: 4736 6466 4736
Size: 1730 5117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2511 2836 3450 3750 4202 844
Size: 1667 325 614 300 452 3489
Fragments arranged by size:
3489 1667 614 452 325 300
BsrBI CCG'CTC
Cuts at: 1394 1823 2734 4734 1394
Size: 429 911 2000 3507
Fragments arranged by size:
3507 2000 911 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2364 3814 5342 5516 65
Size: 1183 1116 1450 1528 174 1396
Fragments arranged by size:
1528 1450 1396 1183 1116 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2026 2441 3370 5491 5678 1700
Size: 110 216 415 929 2121 187 2869
Fragments arranged by size:
2869 2121 929 415 216 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6847
BssHII G'CGCG_C
Cuts at: 1925 3419 1925
Size: 1494 5353
BssSI C'ACGA_G
Cuts at: 4907 5919 4907
Size: 1012 5835
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6847
BstBI TT'CG_AA
Cuts at: 5651 5651
Size: 6847
BstEII G'GTnAC_C
Cuts at: 1184 2665 1184
Size: 1481 5366
BstXI CCAn_nnnn'nTGG
Cuts at: 2080 2080
Size: 6847
BstYI r'GATC_y
Cuts at: 1972 3051 3198 3585 4087 4177 4544 4929 4946
Size: 1079 147 387 502 90 367 385 17
Cuts at: 4946 6398 6484 6496 6842 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1079 502 387 385 367 346
147 90 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2268 3144 513
Size: 552 1203 876 4216
Fragments arranged by size:
4216 1203 876 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2161 487
Size: 683 56 935 5173
Fragments arranged by size:
5173 935 683 56
BtsI GCAGTG_nn'
Cuts at: 4363 5157 5177 4363
Size: 794 20 6033
Fragments arranged by size:
6033 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6847
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6812
DraI TTT'AAA
Cuts at: 4151 4151
Size: 6847
DraIII CAC_nnn'GTG
Cuts at: 2404 2404
Size: 6847
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4010 5854 809
Size: 507 2694 1844 1802
Fragments arranged by size:
2694 1844 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2416 3307
Size: 54 1425 50 275 18 531 55 891
Cuts at: 3307 3372 3402 3930 4294 5183 6812 8
Size: 65 30 528 364 889 1629 43
Fragments arranged by size:
1629 1425 891 889 531 528 364 275
65 55 54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 3372 4294 1537
Size: 275 1495 65 922 4090
Fragments arranged by size:
4090 1495 922 275 65
EarI CTCTTCn'nnn_
Cuts at: 1353 2174 3548 3618 3659 4777 1353
Size: 821 1374 70 41 1118 3423
Fragments arranged by size:
3423 1374 1118 821 70 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3455 5434 5808 5954 387
Size: 1064 138 1866 1979 374 146 1280
Fragments arranged by size:
1979 1866 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3976 3976
Size: 6847
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2584 2750 2752 2944 3068 3640 3742
Size: 393 952 166 2 192 124 572 102
Cuts at: 3742 4910 5507 6294 6562 1239
Size: 1168 597 787 268 1524
Fragments arranged by size:
1524 1168 952 787 597 572 393 268
192 166 124 102 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 3446 2120
Size: 1326 5521
EcoO109I rG'GnC_Cy
Cuts at: 2163 2218 2225 2614 3346 3649 2163
Size: 55 7 389 732 303 5361
Fragments arranged by size:
5361 732 389 303 55 7
EcoRI G'AATT_C
Cuts at: 4156 4534 4156
Size: 378 6469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3409 3670 3409
Size: 261 6586
FspI TGC'GCA
Cuts at: 2687 5353 2687
Size: 2666 4181
HaeII r_GCGC'y
Cuts at: 1678 2571 2729 3301 5994 1678
Size: 893 158 572 2693 2531
Fragments arranged by size:
2693 2531 893 572 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2603 2635 2921 2953 3230
Size: 32 135 32 1031 32 286 32 277
Cuts at: 3230 3262 5488 5520 5562 5594 1373
Size: 32 2226 32 42 32 2626
Fragments arranged by size:
2626 2226 1031 286 277 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4015 4191 4437 669
Size: 988 281 2077 176 246 3079
Fragments arranged by size:
3079 2077 988 281 246 176
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6847
HpaI GTT'AAC
Cuts at: 4437 4437
Size: 6847
KpnI G_GTAC'C
Cuts at: 2198 4172 2198
Size: 1974 4873
MfeI C'AATT_G
Cuts at: 4446 4446
Size: 6847
MluI A'CGCG_T
Cuts at: 6723 6723
Size: 6847
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 5961 6145 1305
Size: 684 3972 184 2007
Fragments arranged by size:
3972 2007 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3404 3932 10
Size: 54 1425 343 1572 528 2925
Fragments arranged by size:
2925 1572 1425 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2042 2100 4805 5164 5323 518
Size: 648 861 15 58 2705 359 159 2042
Fragments arranged by size:
2705 2042 861 648 359 159 58 15
MspA1I CmG'CkG
Cuts at: 1606 2068 2137 2461 3146 3664 4132 4263 4940
Size: 462 69 324 685 518 468 131 677
Cuts at: 4940 6088 6333 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 685 677 518 468 462 324
245 131 69
NaeI GCC'GGC
Cuts at: 2443 3372 2443
Size: 929 5918
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6295
NdeI CA'TA_TG
Cuts at: 387 2962 387
Size: 2575 4272
NgoMIV G'CCGG_C
Cuts at: 2441 3370 2441
Size: 929 5918
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6847
NotI GC'GGCC_GC
Cuts at: 4294 4294
Size: 6847
NspI r_CATG'y
Cuts at: 1132 1229 4205 5750 1132
Size: 97 2976 1545 2229
Fragments arranged by size:
2976 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2895 1348
Size: 1547 5300
PciI A'CATG_T
Cuts at: 5746 5746
Size: 6847
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2206 3177 1108
Size: 1098 971 4778
Fragments arranged by size:
4778 1098 971
PmeI GTTT'AAAC
Cuts at: 4151 4151
Size: 6847
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 4916 4948 6429 6461 4043
Size: 32 841 32 1481 32 4429
Fragments arranged by size:
4429 1481 841 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2163 2218 2225 3346 2163
Size: 55 7 1121 5664
Fragments arranged by size:
5664 1121 55 7
PshAI GACnn'nnGTC
Cuts at: 1613 2223 1613
Size: 610 6237
PsiI TTA'TAA
Cuts at: 4417 4417
Size: 6847
PstI C_TGCA'G
Cuts at: 830 2157 3257 4199 830
Size: 1327 1100 942 3478
Fragments arranged by size:
3478 1327 1100 942
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6847
PvuII CAG'CTG
Cuts at: 2137 2461 3664 4132 2137
Size: 324 1203 468 4852
Fragments arranged by size:
4852 1203 468 324
SacI G_AGCT'C
Cuts at: 3978 3978
Size: 6847
SacII CC_GC'GG
Cuts at: 3147 3147
Size: 6847
SalI G'TCGA_C
Cuts at: 1936 4013 4189 1936
Size: 2077 176 4594
Fragments arranged by size:
4594 2077 176
SanDI GG'GwC_CC
Cuts at: 2163 2225 2163
Size: 62 6785
SbfI CC_TGCA'GG
Cuts at: 4199 4199
Size: 6847
ScaI AGT'ACT
Cuts at: 5095 6807 5095
Size: 1712 5135
SfcI C'TryA_G
Cuts at: 826 1045 2153 3253 4195 5330 6011 6202 826
Size: 219 1108 1100 942 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 1100 942 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
SgrAI Cr'CCGG_yG
Cuts at: 1700 2026 1700
Size: 326 6521
SmaI CCC'GGG
Cuts at: 1872 3478 4174 1872
Size: 1606 696 4545
Fragments arranged by size:
4545 1606 696
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2576 2720 3077 4951 5852 6114
Size: 197 926 633 144 357 1874 901 262
Cuts at: 6114 820
Size: 1553
Fragments arranged by size:
1874 1553 926 901 633 357 262 197
144
SnaBI TAC'GTA
Cuts at: 493 4638 493
Size: 4145 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6847
SphI G_CATG'C
Cuts at: 1132 4205 1132
Size: 3073 3774
SspI AAT'ATT
Cuts at: 5 52 4771 5
Size: 47 4719 2081
Fragments arranged by size:
4719 2081 47
StuI AGG'CCT
Cuts at: 1517 2183 2585 1517
Size: 666 402 5779
Fragments arranged by size:
5779 666 402
StyI C'CwwG_G
Cuts at: 513 1065 2592 3750 4286 513
Size: 552 1527 1158 536 3074
Fragments arranged by size:
3074 1527 1158 552 536
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2470 3169 4874 4891 5044 5229 5674
Size: 1255 890 699 1705 17 153 185 445
Cuts at: 5674 325
Size: 1498
Fragments arranged by size:
1705 1498 1255 890 699 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 5093 6805
Size: 274 80 33 51 1443 54 3062 1712
Cuts at: 6805 96
Size: 138
Fragments arranged by size:
3062 1712 1443 274 138 80 54 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6807 6839 6807
Size: 32 6815
Tth111I GACn'n_nGTC
Cuts at: 1316 2489 1316
Size: 1173 5674
XbaI T'CTAG_A
Cuts at: 2776 4183 2776
Size: 1407 5440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 3174 2113
Size: 1061 5786
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6847
XmaI C'CCGG_G
Cuts at: 1870 3476 4172 1870
Size: 1606 696 4545
Fragments arranged by size:
4545 1606 696
XmnI GAAnn'nnTTC
Cuts at: 4976 4976
Size: 6847
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4655 276
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI