Restriction map for
pFN21AB3557
(Circular) (Six-base) MAPSORT of: pFN21AB3557.seq Check: 1368 from: 1 to: 5605
pFN21AB3557 5605 bp
With 182 enzymes: SgfI *
October 20, 2009 10:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2948 1937
Size: 1011 4594
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2445 3668 5052 5320 1632
Size: 416 397 1223 1384 268 1917
Fragments arranged by size:
1917 1384 1223 416 397 268
AflII C'TTAA_G
Cuts at: 820 1017 2875 820
Size: 197 1858 3550
Fragments arranged by size:
3550 1858 197
AflIII A'CryG_T
Cuts at: 1203 2286 4504 5481 1203
Size: 1083 2218 977 1327
Fragments arranged by size:
2218 1327 1083 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 2690 1166
Size: 1524 4081
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2708 1184
Size: 1524 4081
AlwNI CAG_nnn'CTG
Cuts at: 3348 4920 3348
Size: 1572 4033
ApaLI G'TGCA_C
Cuts at: 3662 4818 3662
Size: 1156 4449
ApoI r'AATT_y
Cuts at: 1493 2034 2914 3140 3292 5401 1493
Size: 541 880 226 152 2109 1697
Fragments arranged by size:
2109 1697 880 541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2930 1870
Size: 73 987 4545
Fragments arranged by size:
4545 987 73
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2926 4381 618
Size: 325 217 627 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2550 2924 1391
Size: 1159 374 4072
Fragments arranged by size:
4072 1159 374
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2018 2211 928
Size: 971 119 193 4322
Fragments arranged by size:
4322 971 193 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 2323 1548
Size: 775 4830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2623 5600 2623
Size: 2977 2628
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 3666 4822 1790
Size: 1876 1156 2573
Fragments arranged by size:
2573 1876 1156
BmgBI CAC'GTC
Cuts at: 1318 2225 1318
Size: 907 4698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3336 4294 442
Size: 416 53 83 399 24 1919 958 1753
Fragments arranged by size:
1919 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2419 4265 1239
Size: 1180 1846 2579
Fragments arranged by size:
2579 1846 1180
Bpu10I CC'TnA_GC
Cuts at: 2850 2850
Size: 5605
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2001 2777 3730 4595 4893 2001
Size: 776 953 865 298 2713
Fragments arranged by size:
2713 953 865 776 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2492 4268 882
Size: 617 993 1776 2219
Fragments arranged by size:
2219 1776 993 617
BsaAI yAC'GTr
Cuts at: 493 1204 2287 2837 3396 493
Size: 711 1083 550 559 2702
Fragments arranged by size:
2702 1083 711 559 550
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3412 3794 4429 275
Size: 53 83 186 936 1879 382 635 1451
Fragments arranged by size:
1879 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5380
Fragments arranged by size:
5380 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5605
BseYI C'CCAG_C
Cuts at: 4808 5304 4808
Size: 496 5109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2714 1163
Size: 573 978 4054
Fragments arranged by size:
4054 978 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3666 3751 4822 3666
Size: 85 1071 4449
Fragments arranged by size:
4449 1071 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2550 2924 3666 3751 4822 1391
Size: 399 760 374 742 85 1071 2174
Fragments arranged by size:
2174 1071 760 742 399 374 85
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2960 844
Size: 2116 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4100 4274 65
Size: 1183 2852 174 1396
Fragments arranged by size:
2852 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4249 4436 1700
Size: 110 2439 187 2869
Fragments arranged by size:
2869 2439 187 110
BsrGI T'GTAC_A
Cuts at: 96 2746 96
Size: 2650 2955
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5605
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2623 2935 3302 3687 3704 5156 5242 5254
Size: 651 312 367 385 17 1452 86 12
Cuts at: 5254 5600 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 651 385 367 346 312 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 2343 3121 3915 3935 2343
Size: 778 794 20 4013
Fragments arranged by size:
4013 794 778 20
ClaI AT'CG_AT
Cuts at: 1554 2193 1554
Size: 639 4966
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2523 2909 2523
Size: 386 5219
DraIII CAC_nnn'GTG
Cuts at: 2840 2840
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3052 3941 5570
Size: 54 1425 50 275 18 1222 889 1629
Cuts at: 5570 8
Size: 43
Fragments arranged by size:
1629 1425 1222 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3535 1353
Size: 2182 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2419 2445 3668 4265 5052 5320
Size: 393 416 371 26 1223 597 787 268
Cuts at: 5320 1239
Size: 1524
Fragments arranged by size:
1524 1223 787 597 416 393 371 268
26
EcoRI G'AATT_C
Cuts at: 2034 2914 3292 2034
Size: 880 378 4347
Fragments arranged by size:
4347 880 378
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 4752 1678
Size: 3074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4246 4278 4320 4352 1373
Size: 32 135 32 2674 32 42 32 2626
Fragments arranged by size:
2674 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2342 748
Size: 1594 4011
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2245 4719 4903 1305
Size: 940 2474 184 2007
Fragments arranged by size:
2474 2007 940 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3783
Fragments arranged by size:
3783 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2331 2690 3563 3922 4081 518
Size: 648 1165 359 873 359 159 2042
Fragments arranged by size:
2042 1165 873 648 359 359 159
MspA1I CmG'CkG
Cuts at: 1606 3021 3698 4846 5091 1606
Size: 1415 677 1148 245 2120
Fragments arranged by size:
2120 1415 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4114
Fragments arranged by size:
4114 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2963 4508 1132
Size: 97 1734 1545 2229
Fragments arranged by size:
2229 1734 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2362 1108
Size: 1254 4351
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PmlI CAC'GTG
Cuts at: 2837 2837
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2624 2656 3674 3706 5187 5219 2624
Size: 32 1018 32 1481 32 3010
Fragments arranged by size:
3010 1481 1018 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2454 2957 830
Size: 1624 503 3478
Fragments arranged by size:
3478 1624 503
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SexAI A'CCwGG_T
Cuts at: 2071 2071
Size: 5605
SfcI C'TryA_G
Cuts at: 826 1045 2141 2450 2953 4088 4769 4960 826
Size: 219 1096 309 503 1135 681 191 1471
Fragments arranged by size:
1471 1135 1096 681 503 309 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2932 1872
Size: 1060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2016 2756 2875 3709 4610 4872
Size: 197 926 73 740 119 834 901 262
Cuts at: 4872 820
Size: 1553
Fragments arranged by size:
1553 926 901 834 740 262 197 119
73
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 2385 2677 1517
Size: 868 292 4445
Fragments arranged by size:
4445 868 292
StyI C'CwwG_G
Cuts at: 513 1065 2004 2296 3044 513
Size: 552 939 292 748 3074
Fragments arranged by size:
3074 939 748 552 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2343 2702 3632 3649 3802 3987 4432
Size: 1255 763 359 930 17 153 185 445
Cuts at: 4432 325
Size: 1498
Fragments arranged by size:
1498 1255 930 763 445 359 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2746 3851 5563
Size: 274 80 33 51 1443 769 1105 1712
Cuts at: 5563 96
Size: 138
Fragments arranged by size:
1712 1443 1105 769 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2041 2941 2041
Size: 900 4705
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2249 2249
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2930 1870
Size: 1060 4545
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI