Restriction map for
pFN21AB3552
(Circular) (Six-base) MAPSORT of: pFN21AB3552.seq Check: 9639 from: 1 to: 5824
pFN21AB3552 5824 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2099 2228 2099
Size: 129 5695
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3167 1937
Size: 1230 4594
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 3887 5271 5539 1632
Size: 422 1833 1384 268 1917
Fragments arranged by size:
1917 1833 1384 422 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 2194 1166
Size: 1028 4796
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2483 2514 1184
Size: 1299 31 4494
Fragments arranged by size:
4494 1299 31
AlwNI CAG_nnn'CTG
Cuts at: 2185 2632 2737 2842 3080 3121 3567 5139 2185
Size: 447 105 105 238 41 446 1572 2870
Fragments arranged by size:
2870 1572 447 446 238 105 105 41
ApaI G_GGCC'C
Cuts at: 2271 2694 2800 2942 2960 2271
Size: 423 106 142 18 5135
Fragments arranged by size:
5135 423 142 106 18
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2393 2556 3033 3149 1870
Size: 73 450 163 477 116 4545
Fragments arranged by size:
4545 477 450 163 116 73
AvrII C'CTAG_G
Cuts at: 3026 3026
Size: 5824
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 3017 3145 4600 618
Size: 325 217 627 420 810 128 1455 1842
Fragments arranged by size:
1842 1455 810 627 420 325 217 128
BanII G_rGCy'C
Cuts at: 1391 2271 2376 2484 2694 2800 2865 2942 2960
Size: 880 105 108 210 106 65 77 18
Cuts at: 2960 3143 1391
Size: 183 4072
Fragments arranged by size:
4072 880 210 183 108 106 105 77
65 18
BbeI G_GCGC'C
Cuts at: 2211 2211
Size: 5824
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4853
BbvCI CC'TCA_GC
Cuts at: 2502 2502
Size: 5824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2144 2178 4015 4049 1903
Size: 34 207 34 1837 34 3678
Fragments arranged by size:
3678 1837 207 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2136 2657 2804
Size: 107 122 71 631 605 464 521 147
Cuts at: 2804 2825 2926 2946 4435 136
Size: 21 101 20 1489 1525
Fragments arranged by size:
1525 1489 631 605 521 464 147 122
107 101 71 21 20
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 2607 2661 3241 2607
Size: 54 580 5190
Fragments arranged by size:
5190 580 54
Bme1580I G_kGCm'C
Cuts at: 1790 2271 2653 2694 2800 2942 2960 3022 3885
Size: 481 382 41 106 142 18 62 863
Cuts at: 3885 5041 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 863 481 382 142 106 62
41 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3555 4513 442
Size: 416 53 83 399 24 2138 958 1753
Fragments arranged by size:
2138 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2498 2675 2822 2864 2952 3083 4484 1239
Size: 1259 177 147 42 88 131 1401 2579
Fragments arranged by size:
2579 1401 1259 177 147 131 88 42
Bpu10I CC'TnA_GC
Cuts at: 2483 2502 2640 2829 2835 2483
Size: 19 138 189 6 5472
Fragments arranged by size:
5472 189 138 19 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3949 4814 5112 3949
Size: 865 298 4661
Fragments arranged by size:
4661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2080 4487 882
Size: 617 581 2407 2219
Fragments arranged by size:
2407 2219 617 581
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2208 3631 4013 4648
Size: 53 83 186 936 675 1423 382 635
Cuts at: 4648 275
Size: 1451
Fragments arranged by size:
1451 1423 936 675 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 1178
Size: 30 165 30 762 30 4807
Fragments arranged by size:
4807 762 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2281 2519 2861 2993 1186
Size: 1095 238 342 132 4017
Fragments arranged by size:
4017 1095 342 238 132
BseYI C'CCAG_C
Cuts at: 2270 2279 2362 2405 2509 3021 3108 5027 5523
Size: 9 83 43 104 512 87 1919 496
Cuts at: 5523 2270
Size: 2571
Fragments arranged by size:
2571 1919 512 496 104 87 83 43
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2443 2510 2711 2789 1163
Size: 573 438 269 67 201 78 4198
Fragments arranged by size:
4198 573 438 269 201 78 67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2484 2865 3885 3970 5041 2484
Size: 381 1020 85 1071 3267
Fragments arranged by size:
3267 1071 1020 381 85
BsmI GAATG_Cn'
Cuts at: 2503 3335 3428 2503
Size: 832 93 4899
Fragments arranged by size:
4899 832 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2271 2376 2484 2653 2694 2800 2865
Size: 399 481 105 108 169 41 106 65
Cuts at: 2865 2942 2960 3022 3143 3885 3970 5041 1391
Size: 77 18 62 121 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 481 399 169 121 108
106 105 85 77 65 62 41 18
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 2228 2475 2886 3179 844
Size: 1255 129 247 411 293 3489
Fragments arranged by size:
3489 1255 411 293 247 129
BsrBI CCG'CTC
Cuts at: 1394 1823 2176 2752 3711 1394
Size: 429 353 576 959 3507
Fragments arranged by size:
3507 959 576 429 353
BsrDI GCAATG_nn'
Cuts at: 65 1248 4319 4493 65
Size: 1183 3071 174 1396
Fragments arranged by size:
3071 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4468 4655 1700
Size: 110 2658 187 2869
Fragments arranged by size:
2869 2658 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2185 2539 2594 3080 794
Size: 1391 354 55 486 3538
Fragments arranged by size:
3538 1391 486 354 55
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819
Size: 1182 367 385 17 1452 86 12 346
Cuts at: 5819 1972
Size: 1977
Fragments arranged by size:
1977 1452 1182 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2255 2255
Size: 5824
BtgI C'CryG_G
Cuts at: 513 1065 2025 2351 2924 513
Size: 552 960 326 573 3413
Fragments arranged by size:
3413 960 573 552 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2092 2253 487
Size: 683 56 866 161 4058
Fragments arranged by size:
4058 866 683 161 56
BtsI GCAGTG_nn'
Cuts at: 2601 3340 4134 4154 2601
Size: 739 794 20 4271
Fragments arranged by size:
4271 794 739 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DraIII CAC_nnn'GTG
Cuts at: 2594 2594
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2137 2598
Size: 54 1425 50 275 18 192 115 461
Cuts at: 2598 3271 4160 5789 8
Size: 673 889 1629 43
Fragments arranged by size:
1629 1425 889 673 461 275 192 115
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3754 1353
Size: 2401 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2482 2863 2482
Size: 381 5443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2498 2675 2822 2864 2952 3083
Size: 393 422 444 177 147 42 88 131
Cuts at: 3083 3887 4484 5271 5539 1239
Size: 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 597 444 422 393 268
177 147 131 88 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2729 2729
Size: 5824
EcoO109I rG'GnC_Cy
Cuts at: 2267 2286 2473 2629 2683 2691 2734 2797 2938
Size: 19 187 156 54 8 43 63 141
Cuts at: 2938 2939 2956 2957 3030 2267
Size: 1 17 1 73 5061
Fragments arranged by size:
5061 187 156 141 73 63 54 43
19 17 8 1 1
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2211 2725 2997 4971 1678
Size: 533 514 272 1974 2531
Fragments arranged by size:
2531 1974 533 514 272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4465 4497 4539 4571 1373
Size: 32 135 32 2893 32 42 32 2626
Fragments arranged by size:
2893 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KasI G'GCGC_C
Cuts at: 2207 2207
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4002
Fragments arranged by size:
4002 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2194 3015 3782 4141 4300 518
Size: 648 1028 821 767 359 159 2042
Fragments arranged by size:
2042 1028 821 767 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2301 2671 2678 3240 3917 5065 5310
Size: 552 143 370 7 562 677 1148 245
Cuts at: 5310 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 562 552 370 245 143
7
NarI GG'CG_CC
Cuts at: 2208 2208
Size: 5824
NcoI C'CATG_G
Cuts at: 513 1065 2025 513
Size: 552 960 4312
Fragments arranged by size:
4312 960 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2615 3182 4727 1132
Size: 97 1386 567 1545 2229
Fragments arranged by size:
2229 1545 1386 567 97
PasI CC'CwG_GG
Cuts at: 1348 2420 2687 1348
Size: 1072 267 4485
Fragments arranged by size:
4485 1072 267
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2062 2101 2737 3121 1108
Size: 954 39 636 384 3811
Fragments arranged by size:
3811 954 636 384 39
PfoI T'CCnGG_A
Cuts at: 3060 3060
Size: 5824
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2734 2734
Size: 5824
PshAI GACnn'nnGTC
Cuts at: 1613 2301 1613
Size: 688 5136
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2267 2690 2796 2938 2956 2267
Size: 423 106 142 18 5135
Fragments arranged by size:
5135 423 142 106 18
PstI C_TGCA'G
Cuts at: 830 2586 2628 2670 2883 3176 830
Size: 1756 42 42 213 293 3478
Fragments arranged by size:
3478 1756 293 213 42 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2301 2671 2301
Size: 370 5454
SacI G_AGCT'C
Cuts at: 2484 2865 2484
Size: 381 5443
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SbfI CC_TGCA'GG
Cuts at: 2586 3176 2586
Size: 590 5234
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 2582 2624 2666 2879 3172 4307 4988
Size: 219 1537 42 42 213 293 1135 681
Cuts at: 4988 5179 826
Size: 191 1471
Fragments arranged by size:
1537 1471 1135 681 293 219 213 191
42 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 2136 2136
Size: 5824
SfoI GGC'GCC
Cuts at: 2209 2209
Size: 5824
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 2558 3151 1872
Size: 686 593 4545
Fragments arranged by size:
4545 686 593
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3928 4829 5091 820
Size: 197 926 1985 901 262 1553
Fragments arranged by size:
1985 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 2615 3182 1132
Size: 1483 567 3774
Fragments arranged by size:
3774 1483 567
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 2522 1517
Size: 1005 4819
StyI C'CwwG_G
Cuts at: 513 1065 2025 3026 3263 513
Size: 552 960 1001 237 3074
Fragments arranged by size:
3074 1001 960 552 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2032 2458 3851 3868 4021 4206 4651
Size: 1255 452 426 1393 17 153 185 445
Cuts at: 4651 325
Size: 1498
Fragments arranged by size:
1498 1393 1255 452 445 426 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4070 5782 96
Size: 274 80 33 51 1443 2093 1712 138
Fragments arranged by size:
2093 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5784 5816 5784
Size: 32 5792
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 2556 3149 1870
Size: 686 593 4545
Fragments arranged by size:
4545 686 593
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SapI SexAI SrfI SwaI XcmI